Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_I18
(875 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O57590|SURF4_FUGRU Surfeit locus protein 4 163 7e-40
sp|Q64310|SURF4_MOUSE Surfeit locus protein 4 158 2e-38
sp|O15260|SURF4_HUMAN Surfeit locus protein 4 157 3e-38
sp|Q18864|SURF4_CAEEL Surfeit locus protein 4 homolog 154 2e-37
sp|O18405|SURF4_DROME Surfeit locus protein 4 homolog 140 5e-33
sp|O74559|SURF4_SCHPO Surfeit locus protein 4 homolog 94 6e-19
sp|P53337|YG5Y_YEAST Hypothetical 35.0 kDa protein in BGL2-... 91 3e-18
sp|O45731|YFC7_CAEEL Hypothetical protein T02E1.7 in chromo... 83 1e-15
sp|P0AD48|YPHA_SHIFL Inner membrane protein yphA >gi|825925... 39 0.025
sp|P04372|COX2_TRYBB Cytochrome c oxidase subunit 2 (Cytoch... 33 1.0
>sp|O57590|SURF4_FUGRU Surfeit locus protein 4
Length = 269
Score = 163 bits (412), Expect = 7e-40
Identities = 78/136 (57%), Positives = 103/136 (75%)
Frame = +3
Query: 3 SNEKVKSYLAGLPNTGINQPKQYMLLGGRLLVVLIFITLIHWDGGFFYMIQTLANLVLIL 182
S + KS AG+P+ G + PKQYM LGGR+L+VL+F+TL+H+D FF ++Q L LI+
Sbjct: 134 SRSEGKSMFAGVPSMGESSPKQYMQLGGRVLLVLMFMTLLHFDFNFFSILQNLVGTALII 193
Query: 183 MVAIGYKTKLCAIILVLWLTTMNFWYNNFWYYIDRELLWDFLKYDFFQTWSVIGGLLLIV 362
+VAIG+KTKL A+ LVLWL +N ++N FW + + DFLKYDFFQT SVIGGLLL+V
Sbjct: 194 LVAIGFKTKLAALTLVLWLLVINVYFNAFWTIPAYKPMHDFLKYDFFQTTSVIGGLLLVV 253
Query: 363 AYGPGGVSIDDYKKDW 410
A GPGGVS+D+ KK+W
Sbjct: 254 ALGPGGVSMDEKKKEW 269
>sp|Q64310|SURF4_MOUSE Surfeit locus protein 4
Length = 269
Score = 158 bits (399), Expect = 2e-38
Identities = 75/136 (55%), Positives = 101/136 (74%)
Frame = +3
Query: 3 SNEKVKSYLAGLPNTGINQPKQYMLLGGRLLVVLIFITLIHWDGGFFYMIQTLANLVLIL 182
S + KS AG+P + PKQYM LGGR+L+VL+F+TL+H+D FF +IQ + L++
Sbjct: 134 SRSEGKSMFAGVPTMRESSPKQYMQLGGRVLLVLMFMTLLHFDASFFSIIQNIVGTALMI 193
Query: 183 MVAIGYKTKLCAIILVLWLTTMNFWYNNFWYYIDRELLWDFLKYDFFQTWSVIGGLLLIV 362
+VAIG+KTKL A+ LV+WL +N ++N FW + + DFLKYDFFQT SVIGGLLL+V
Sbjct: 194 LVAIGFKTKLAALTLVVWLFAINVYFNAFWTIPVYKPMHDFLKYDFFQTMSVIGGLLLVV 253
Query: 363 AYGPGGVSIDDYKKDW 410
A GPGGVS+D+ KK+W
Sbjct: 254 ALGPGGVSMDEKKKEW 269
>sp|O15260|SURF4_HUMAN Surfeit locus protein 4
Length = 269
Score = 157 bits (398), Expect = 3e-38
Identities = 74/136 (54%), Positives = 101/136 (74%)
Frame = +3
Query: 3 SNEKVKSYLAGLPNTGINQPKQYMLLGGRLLVVLIFITLIHWDGGFFYMIQTLANLVLIL 182
S + KS AG+P + PKQYM LGGR+L+VL+F+TL+H+D FF ++Q + L++
Sbjct: 134 SRSEGKSMFAGVPTMRESSPKQYMQLGGRVLLVLMFMTLLHFDASFFSIVQNIVGTALMI 193
Query: 183 MVAIGYKTKLCAIILVLWLTTMNFWYNNFWYYIDRELLWDFLKYDFFQTWSVIGGLLLIV 362
+VAIG+KTKL A+ LV+WL +N ++N FW + + DFLKYDFFQT SVIGGLLL+V
Sbjct: 194 LVAIGFKTKLAALTLVVWLFAINVYFNAFWTIPVYKPMHDFLKYDFFQTMSVIGGLLLVV 253
Query: 363 AYGPGGVSIDDYKKDW 410
A GPGGVS+D+ KK+W
Sbjct: 254 ALGPGGVSMDEKKKEW 269
>sp|Q18864|SURF4_CAEEL Surfeit locus protein 4 homolog
Length = 277
Score = 154 bits (390), Expect = 2e-37
Identities = 71/137 (51%), Positives = 98/137 (71%), Gaps = 1/137 (0%)
Frame = +3
Query: 3 SNEKVKSYLAGLPNTG-INQPKQYMLLGGRLLVVLIFITLIHWDGGFFYMIQTLANLVLI 179
+ E+ S AG+P G N+PK YMLL GR+L++ +F++L+H++ F +++ + LI
Sbjct: 141 TQEEKASLFAGVPTMGDSNKPKSYMLLAGRVLLIFMFMSLMHFEMSFMQVLEIVVGFALI 200
Query: 180 LMVAIGYKTKLCAIILVLWLTTMNFWYNNFWYYIDRELLWDFLKYDFFQTWSVIGGLLLI 359
+V+IGYKTKL AI+LV+WL +N W N +W DF+KYDFFQT SVIGGLLL+
Sbjct: 201 TLVSIGYKTKLSAIVLVIWLFGLNLWLNAWWTIPSDRFYRDFMKYDFFQTMSVIGGLLLV 260
Query: 360 VAYGPGGVSIDDYKKDW 410
+AYGPGGVS+DDYKK W
Sbjct: 261 IAYGPGGVSVDDYKKRW 277
>sp|O18405|SURF4_DROME Surfeit locus protein 4 homolog
Length = 270
Score = 140 bits (353), Expect = 5e-33
Identities = 67/131 (51%), Positives = 96/131 (73%)
Frame = +3
Query: 18 KSYLAGLPNTGINQPKQYMLLGGRLLVVLIFITLIHWDGGFFYMIQTLANLVLILMVAIG 197
+S AG+P+ G N+PK +M L GR+L+ +FITLI ++ + +IQ + +L+++V +G
Sbjct: 140 RSLFAGVPSMGENKPKNFMQLAGRILLAFMFITLIRFELSVWQVIQDIIGSILMVLVVLG 199
Query: 198 YKTKLCAIILVLWLTTMNFWYNNFWYYIDRELLWDFLKYDFFQTWSVIGGLLLIVAYGPG 377
YKTKL A+ILV LT +N ++N +W + L DFLKYDFFQT SVIGGLL+IV+ GPG
Sbjct: 200 YKTKLSALILVALLTILNLYHNAWWTIPSYKPLRDFLKYDFFQTLSVIGGLLMIVSLGPG 259
Query: 378 GVSIDDYKKDW 410
GVS+D++KK W
Sbjct: 260 GVSMDEHKKKW 270
>sp|O74559|SURF4_SCHPO Surfeit locus protein 4 homolog
Length = 302
Score = 93.6 bits (231), Expect = 6e-19
Identities = 50/128 (39%), Positives = 74/128 (57%), Gaps = 3/128 (2%)
Frame = +3
Query: 30 AGLPNTGINQPKQYMLLGGRLLVVLIFITLIHWDGGFFYMIQTLANLVLIL---MVAIGY 200
AGLP + + Y L GR+L++ +F+ L+ +G + L +++ + MV IG+
Sbjct: 173 AGLPAVSEHNKRTYFQLAGRVLLIFMFLGLLAKEGSGISWTRILVHILSVTACAMVVIGF 232
Query: 201 KTKLCAIILVLWLTTMNFWYNNFWYYIDRELLWDFLKYDFFQTWSVIGGLLLIVAYGPGG 380
K K A +LVL L+ NF N+FW DF +YDFFQT S++GGLL +V GPG
Sbjct: 233 KAKFFAAVLVLILSVANFIINSFWSVpreSPYRDFYRYDFFQTLSIVGGLLYLVNTGPGK 292
Query: 381 VSIDDYKK 404
S+D+ KK
Sbjct: 293 FSVDEKKK 300
>sp|P53337|YG5Y_YEAST Hypothetical 35.0 kDa protein in BGL2-ZUO1 intergenic region
Length = 310
Score = 91.3 bits (225), Expect = 3e-18
Identities = 50/121 (41%), Positives = 76/121 (62%)
Frame = +3
Query: 42 NTGINQPKQYMLLGGRLLVVLIFITLIHWDGGFFYMIQTLANLVLILMVAIGYKTKLCAI 221
N+ ++ K Y+L GR+L+VL+FI + +F ++ T+ + AIGYKTK +I
Sbjct: 194 NSKNDKAKGYLLFAGRILIVLMFIAFT-FSKSWFTVVLTIIGTICF---AIGYKTKFASI 249
Query: 222 ILVLWLTTMNFWYNNFWYYIDRELLWDFLKYDFFQTWSVIGGLLLIVAYGPGGVSIDDYK 401
+L L LT N NN+W+Y + + DFLKY+F+Q S+IGGLLL+ G G +S+D+ K
Sbjct: 250 MLGLILTFYNITLNNYWFYNNTKR--DFLKYEFYQNLSIIGGLLLVTNTGAGELSVDEKK 307
Query: 402 K 404
K
Sbjct: 308 K 308
>sp|O45731|YFC7_CAEEL Hypothetical protein T02E1.7 in chromosome I
Length = 269
Score = 82.8 bits (203), Expect = 1e-15
Identities = 41/113 (36%), Positives = 69/113 (61%)
Frame = +3
Query: 72 MLLGGRLLVVLIFITLIHWDGGFFYMIQTLANLVLILMVAIGYKTKLCAIILVLWLTTMN 251
+L R+ + L+ I+++H+D + ++ + + +++ V +G+KT++ + +L WL N
Sbjct: 160 LLAACRVCLNLMLISMVHFDMSYTRILLCIISYGMMIFVWLGFKTRMMSFMLATWLFAYN 219
Query: 252 FWYNNFWYYIDRELLWDFLKYDFFQTWSVIGGLLLIVAYGPGGVSIDDYKKDW 410
N+FW D EL ++YDFFQT S IGGLLL++ GPG S D+ KK W
Sbjct: 220 IVLNDFWNK-DAEL--HIIRYDFFQTLSAIGGLLLLIHTGPGEFSFDELKKKW 269
>sp|P0AD48|YPHA_SHIFL Inner membrane protein yphA
sp|P0AD47|YPHA_ECOLI Inner membrane protein yphA
Length = 140
Score = 38.5 bits (88), Expect = 0.025
Identities = 31/128 (24%), Positives = 60/128 (46%), Gaps = 21/128 (16%)
Frame = +3
Query: 72 MLLGGRLLVVLIFIT-----LIHWDGGFFYM------IQTLANLVLILM-------VAIG 197
+LL R+ VVLIFI ++ +DG YM + LA ++ ++M + +G
Sbjct: 16 LLLIARIAVVLIFIIFGFPKMMGFDGTVQYMASLGAPMPMLAAIIAVVMEVPAAILIVLG 75
Query: 198 YKTKLCAIILVLWLTTMNFWYNNFWYYIDRELLWDFLKYDFFQTW---SVIGGLLLIVAY 368
+ T+ A++ + + +++W ++ D + + W S+ G LL+
Sbjct: 76 FFTRPLAVLFIFYTLGTAVIGHHYW-----DMTGDAVGPNMINFWKNVSIAGAFLLLAIT 130
Query: 369 GPGGVSID 392
GPG +S+D
Sbjct: 131 GPGAISLD 138
>sp|P04372|COX2_TRYBB Cytochrome c oxidase subunit 2 (Cytochrome c oxidase polypeptide
II)
Length = 210
Score = 33.1 bits (74), Expect = 1.0
Identities = 19/74 (25%), Positives = 37/74 (50%), Gaps = 1/74 (1%)
Frame = +3
Query: 141 FYMIQTLANLVLILMVAIGYKTKLCAIILVLWLTTMNFWYNNFWYYIDRELLWDFLKYDF 320
F+MI + ++++++ +I +CA+I+ LT NN + WDF+ F
Sbjct: 7 FWMIFLMDSIIVLISFSIFLSVWICALIIATVLTVTKI--NNIY------CTWDFISSKF 58
Query: 321 FQT-WSVIGGLLLI 359
T W V+G + ++
Sbjct: 59 IDTYWFVLGMMFIL 72
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 89,432,188
Number of Sequences: 369166
Number of extensions: 1681271
Number of successful extensions: 3934
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3825
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3929
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8694177530
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)