Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_G23
(606 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q99JY0|ECHB_MOUSE Trifunctional enzyme beta subunit, mit... 247 1e-65
sp|O46629|ECHB_BOVIN Trifunctional enzyme beta subunit, mit... 247 1e-65
sp|Q60587|ECHB_RAT Trifunctional enzyme beta subunit, mitoc... 246 3e-65
sp|Q5R1W7|ECHB_PANTR Trifunctional enzyme beta subunit, mit... 246 4e-65
sp|Q8HXX4|ECHB_MACFA Trifunctional enzyme beta subunit, mit... 246 4e-65
sp|P34255|YKA3_CAEEL Hypothetical protein B0303.3 in chromo... 239 4e-63
sp|P76503|FADI_ECOLI 3-ketoacyl-CoA thiolase (Fatty acid ox... 144 2e-34
sp|P17764|THIL_RAT Acetyl-CoA acetyltransferase, mitochondr... 97 4e-20
sp|Q8QZT1|THIL_MOUSE Acetyl-CoA acetyltransferase, mitochon... 96 7e-20
sp|Q12598|THIA_CANTR Acetyl-CoA acetyltransferase IA (Perox... 96 9e-20
>sp|Q99JY0|ECHB_MOUSE Trifunctional enzyme beta subunit, mitochondrial precursor
(TP-beta) [Includes: 3-ketoacyl-CoA thiolase (Acetyl-CoA
acyltransferase) (Beta-ketothiolase)]
Length = 475
Score = 247 bits (631), Expect = 1e-65
Identities = 118/164 (71%), Positives = 138/164 (84%)
Frame = +3
Query: 3 FLTDGASACLLTTEEKAKQMGWKPKAYLRDFIYVSQDPKDQLLLGPAYATPKILDRNKLT 182
FLTDGASA L+ +E++A MG+KPKAYLRDFIYVSQDPKDQLLLGP YATPK+L++ LT
Sbjct: 311 FLTDGASAMLIMSEDRALAMGYKPKAYLRDFIYVSQDPKDQLLLGPTYATPKVLEKAGLT 370
Query: 183 LKDITAFEFHEAFAGQILANLKAMDCDFFCKEYMKRPAKYGSLPLDRLNTWGGSLSIGHP 362
+ DI AFEFHEAF+GQILAN KAMD D+F + YM R K GS PL++ N WGGSLS+GHP
Sbjct: 371 MNDIDAFEFHEAFSGQILANFKAMDSDWFAQNYMGRKTKVGSPPLEKFNIWGGSLSLGHP 430
Query: 363 FGATGVRLVTTAANRLIKEKGQFALVAACAAGGQGHAILIENYP 494
FGATG RLV AANRL K+ GQ+ALVAACAAGGQGHA+++E YP
Sbjct: 431 FGATGCRLVMAAANRLRKDGGQYALVAACAAGGQGHAMIVEAYP 474
>sp|O46629|ECHB_BOVIN Trifunctional enzyme beta subunit, mitochondrial precursor
(TP-beta) [Includes: 3-ketoacyl-CoA thiolase (Acetyl-CoA
acyltransferase) (Beta-ketothiolase)]
Length = 475
Score = 247 bits (631), Expect = 1e-65
Identities = 119/164 (72%), Positives = 136/164 (82%)
Frame = +3
Query: 3 FLTDGASACLLTTEEKAKQMGWKPKAYLRDFIYVSQDPKDQLLLGPAYATPKILDRNKLT 182
FLTDGASA L+ EEKA MG+KPKAYLRDF+YVSQDPKDQLLLGP YATPK+L++ LT
Sbjct: 311 FLTDGASAVLIMAEEKALAMGYKPKAYLRDFMYVSQDPKDQLLLGPTYATPKVLEKAGLT 370
Query: 183 LKDITAFEFHEAFAGQILANLKAMDCDFFCKEYMKRPAKYGSLPLDRLNTWGGSLSIGHP 362
+ DI FEFHEAF+GQILANLKAMD D+F + YM R AK G PL++ N WGGSLS+GHP
Sbjct: 371 MNDIDVFEFHEAFSGQILANLKAMDSDWFAQNYMGRKAKVGLPPLEKFNNWGGSLSLGHP 430
Query: 363 FGATGVRLVTTAANRLIKEKGQFALVAACAAGGQGHAILIENYP 494
FGATG RLV AANRL KE GQ+ LVAACAAGGQGHA+++E YP
Sbjct: 431 FGATGCRLVMAAANRLRKEGGQYGLVAACAAGGQGHAMIVEAYP 474
>sp|Q60587|ECHB_RAT Trifunctional enzyme beta subunit, mitochondrial precursor
(TP-beta) [Includes: 3-ketoacyl-CoA thiolase (Acetyl-CoA
acyltransferase) (Beta-ketothiolase)]
Length = 475
Score = 246 bits (628), Expect = 3e-65
Identities = 117/164 (71%), Positives = 138/164 (84%)
Frame = +3
Query: 3 FLTDGASACLLTTEEKAKQMGWKPKAYLRDFIYVSQDPKDQLLLGPAYATPKILDRNKLT 182
FLTDGASA L+ +E++A MG+KPKAYLRDFIYVSQDPKDQLLLGP YATPK+L++ LT
Sbjct: 311 FLTDGASAMLIMSEDRALAMGYKPKAYLRDFIYVSQDPKDQLLLGPTYATPKVLEKAGLT 370
Query: 183 LKDITAFEFHEAFAGQILANLKAMDCDFFCKEYMKRPAKYGSLPLDRLNTWGGSLSIGHP 362
+ DI AFEFHEAF+GQILAN KAMD D+F + YM R K G+ PL++ N WGGSLS+GHP
Sbjct: 371 MNDIDAFEFHEAFSGQILANFKAMDSDWFAQNYMGRKTKVGAPPLEKFNIWGGSLSLGHP 430
Query: 363 FGATGVRLVTTAANRLIKEKGQFALVAACAAGGQGHAILIENYP 494
FGATG RLV AANRL K+ GQ+ALVAACAAGGQGHA+++E YP
Sbjct: 431 FGATGCRLVMAAANRLRKDGGQYALVAACAAGGQGHAMIVEAYP 474
>sp|Q5R1W7|ECHB_PANTR Trifunctional enzyme beta subunit, mitochondrial precursor
(TP-beta) [Includes: 3-ketoacyl-CoA thiolase (Acetyl-CoA
acyltransferase) (Beta-ketothiolase)]
sp|P55084|ECHB_HUMAN Trifunctional enzyme beta subunit, mitochondrial precursor
(TP-beta) [Includes: 3-ketoacyl-CoA thiolase (Acetyl-CoA
acyltransferase) (Beta-ketothiolase)]
Length = 475
Score = 246 bits (627), Expect = 4e-65
Identities = 118/164 (71%), Positives = 135/164 (82%)
Frame = +3
Query: 3 FLTDGASACLLTTEEKAKQMGWKPKAYLRDFIYVSQDPKDQLLLGPAYATPKILDRNKLT 182
FLTDGASA L+ EEKA MG+KPKAYLRDF+YVSQDPKDQLLLGP YATPK+L++ LT
Sbjct: 311 FLTDGASAMLIMAEEKALAMGYKPKAYLRDFMYVSQDPKDQLLLGPTYATPKVLEKAGLT 370
Query: 183 LKDITAFEFHEAFAGQILANLKAMDCDFFCKEYMKRPAKYGSLPLDRLNTWGGSLSIGHP 362
+ DI AFEFHEAF+GQILAN KAMD D+F + YM R K G PL++ N WGGSLS+GHP
Sbjct: 371 MNDIDAFEFHEAFSGQILANFKAMDSDWFAENYMGRKTKVGLPPLEKFNNWGGSLSLGHP 430
Query: 363 FGATGVRLVTTAANRLIKEKGQFALVAACAAGGQGHAILIENYP 494
FGATG RLV AANRL KE GQ+ LVAACAAGGQGHA+++E YP
Sbjct: 431 FGATGCRLVMAAANRLRKEGGQYGLVAACAAGGQGHAMIVEAYP 474
>sp|Q8HXX4|ECHB_MACFA Trifunctional enzyme beta subunit, mitochondrial precursor
(TP-beta) [Includes: 3-ketoacyl-CoA thiolase (Acetyl-CoA
acyltransferase) (Beta-ketothiolase)]
Length = 475
Score = 246 bits (627), Expect = 4e-65
Identities = 118/164 (71%), Positives = 135/164 (82%)
Frame = +3
Query: 3 FLTDGASACLLTTEEKAKQMGWKPKAYLRDFIYVSQDPKDQLLLGPAYATPKILDRNKLT 182
FLTDGASA L+ EEKA MG+KPKAYLRDF+YVSQDPKDQLLLGP YATPK+L++ LT
Sbjct: 311 FLTDGASAMLIMAEEKALAMGYKPKAYLRDFMYVSQDPKDQLLLGPTYATPKVLEKAGLT 370
Query: 183 LKDITAFEFHEAFAGQILANLKAMDCDFFCKEYMKRPAKYGSLPLDRLNTWGGSLSIGHP 362
+ DI AFEFHEAF+GQILAN KAMD D+F + YM R K G PL++ N WGGSLS+GHP
Sbjct: 371 MNDIDAFEFHEAFSGQILANFKAMDSDWFAENYMGRKTKVGLPPLEKFNNWGGSLSLGHP 430
Query: 363 FGATGVRLVTTAANRLIKEKGQFALVAACAAGGQGHAILIENYP 494
FGATG RLV AANRL KE GQ+ LVAACAAGGQGHA+++E YP
Sbjct: 431 FGATGCRLVMAAANRLRKEGGQYGLVAACAAGGQGHAMIVEAYP 474
>sp|P34255|YKA3_CAEEL Hypothetical protein B0303.3 in chromosome III
Length = 448
Score = 239 bits (610), Expect = 4e-63
Identities = 113/163 (69%), Positives = 135/163 (82%)
Frame = +3
Query: 3 FLTDGASACLLTTEEKAKQMGWKPKAYLRDFIYVSQDPKDQLLLGPAYATPKILDRNKLT 182
+LTDGASA L+ TEE A G+KPKAYLRD++YV+QDPKDQLLL PAY PK+LD+ LT
Sbjct: 284 YLTDGASAALIMTEEYALANGYKPKAYLRDYLYVAQDPKDQLLLSPAYVIPKLLDKAGLT 343
Query: 183 LKDITAFEFHEAFAGQILANLKAMDCDFFCKEYMKRPAKYGSLPLDRLNTWGGSLSIGHP 362
LKD+ FE HEAFAGQ+LANL AMD D+FCKE MKR K+G +P+D+LN WGGSLSIGHP
Sbjct: 344 LKDVDVFEIHEAFAGQVLANLNAMDSDYFCKEQMKRSGKFGRVPMDKLNLWGGSLSIGHP 403
Query: 363 FGATGVRLVTTAANRLIKEKGQFALVAACAAGGQGHAILIENY 491
FGATGVRL T +A+RL +EKGQ+A++AACAAGG G +LIE Y
Sbjct: 404 FGATGVRLATHSAHRLKEEKGQYAVIAACAAGGHGVGMLIEAY 446
>sp|P76503|FADI_ECOLI 3-ketoacyl-CoA thiolase (Fatty acid oxidation complex beta subunit)
(Beta-ketothiolase) (Acetyl-CoA acyltransferase)
(Acyl-CoA ligase) (ACSs)
Length = 436
Score = 144 bits (362), Expect = 2e-34
Identities = 70/160 (43%), Positives = 102/160 (63%)
Frame = +3
Query: 6 LTDGASACLLTTEEKAKQMGWKPKAYLRDFIYVSQDPKDQLLLGPAYATPKILDRNKLTL 185
LTDGA+A +L TE +AK++G P YLR + + + D +LLGPA++TP L+R LT+
Sbjct: 275 LTDGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM 334
Query: 186 KDITAFEFHEAFAGQILANLKAMDCDFFCKEYMKRPAKYGSLPLDRLNTWGGSLSIGHPF 365
D+T + HEAFA Q LAN++ + + F +E + R G + + N GGS++ GHPF
Sbjct: 335 SDLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGEVDDSKFNVLGGSIAYGHPF 394
Query: 366 GATGVRLVTTAANRLIKEKGQFALVAACAAGGQGHAILIE 485
ATG R++T + L + G F LV ACAAGG G A+++E
Sbjct: 395 AATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLE 434
>sp|P17764|THIL_RAT Acetyl-CoA acetyltransferase, mitochondrial precursor
(Acetoacetyl-CoA thiolase)
Length = 424
Score = 96.7 bits (239), Expect = 4e-20
Identities = 61/160 (38%), Positives = 89/160 (55%)
Frame = +3
Query: 6 LTDGASACLLTTEEKAKQMGWKPKAYLRDFIYVSQDPKDQLLLGPAYATPKILDRNKLTL 185
L DGA+A +L T E A+++ KP A + F + DP D L PAYA PK+L L
Sbjct: 283 LNDGAAAVVLMTAEAAQRLKVKPLARIAAFADAAVDPID-FPLAPAYAVPKVLKYAGLKK 341
Query: 186 KDITAFEFHEAFAGQILANLKAMDCDFFCKEYMKRPAKYGSLPLDRLNTWGGSLSIGHPF 365
+DI +E +EAF+ +LAN+K ++ D P K +N GG++S+GHP
Sbjct: 342 EDIAMWEVNEAFSVVVLANIKMLEID---------PQK--------VNVHGGAVSLGHPI 384
Query: 366 GATGVRLVTTAANRLIKEKGQFALVAACAAGGQGHAILIE 485
G +G R+V A+ L ++G+F L + C GG A+LIE
Sbjct: 385 GMSGARIVVHLAHAL--KQGEFGLASICNGGGGASAVLIE 422
>sp|Q8QZT1|THIL_MOUSE Acetyl-CoA acetyltransferase, mitochondrial precursor
(Acetoacetyl-CoA thiolase)
Length = 424
Score = 95.9 bits (237), Expect = 7e-20
Identities = 61/160 (38%), Positives = 88/160 (55%)
Frame = +3
Query: 6 LTDGASACLLTTEEKAKQMGWKPKAYLRDFIYVSQDPKDQLLLGPAYATPKILDRNKLTL 185
L DGA+A +L T E A+++ KP A + F + DP D L PAYA PK+L L
Sbjct: 283 LNDGAAALVLMTAEAAQRLNVKPLARIAAFADAAVDPID-FPLAPAYAVPKVLKYAGLKK 341
Query: 186 KDITAFEFHEAFAGQILANLKAMDCDFFCKEYMKRPAKYGSLPLDRLNTWGGSLSIGHPF 365
+DI +E +EAF+ +LAN+K ++ D P K +N GG++S+GHP
Sbjct: 342 EDIAMWEVNEAFSVVVLANIKMLEID---------PQK--------VNIHGGAVSLGHPI 384
Query: 366 GATGVRLVTTAANRLIKEKGQFALVAACAAGGQGHAILIE 485
G +G R+V A+ L + G+F L + C GG A+LIE
Sbjct: 385 GMSGARIVVHMAHAL--KPGEFGLASICNGGGGASALLIE 422
>sp|Q12598|THIA_CANTR Acetyl-CoA acetyltransferase IA (Peroxisomal acetoacetyl-CoA
thiolase) (Thiolase IA)
Length = 403
Score = 95.5 bits (236), Expect = 9e-20
Identities = 54/160 (33%), Positives = 87/160 (54%)
Frame = +3
Query: 6 LTDGASACLLTTEEKAKQMGWKPKAYLRDFIYVSQDPKDQLLLGPAYATPKILDRNKLTL 185
L DG +A +L +E K KQ+G KP A + + ++ P D + PA A PK + LT+
Sbjct: 254 LNDGGAALVLVSEAKLKQLGLKPLAKISGWGEAARTPFD-FTIAPALAVPKAVKHAGLTV 312
Query: 186 KDITAFEFHEAFAGQILANLKAMDCDFFCKEYMKRPAKYGSLPLDRLNTWGGSLSIGHPF 365
+ FE +EAF+ LAN A+ ++PL++LN +GG++++GHP
Sbjct: 313 DRVDFFELNEAFSVVGLAN-----------------AELVNIPLEKLNVYGGAVAMGHPL 355
Query: 366 GATGVRLVTTAANRLIKEKGQFALVAACAAGGQGHAILIE 485
G +G R++ T + L +E G+F + C GG A++IE
Sbjct: 356 GCSGARIIVTLLSVLTQEGGRFGVAGVCNGGGGASAVVIE 395
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 65,472,347
Number of Sequences: 369166
Number of extensions: 1209492
Number of successful extensions: 2862
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2738
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2768
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4699949280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)