Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_G01
(849 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P92177|1433E_DROME 14-3-3 protein epsilon (Suppressor of... 301 2e-81
sp|P93214|14339_LYCES 14-3-3 protein 9 298 2e-80
sp|Q9C5W6|143C_ARATH 14-3-3-like protein GF14 iota (General... 297 2e-80
sp|P48347|14310_ARATH 14-3-3-like protein GF14 epsilon (Gen... 297 2e-80
sp|Q96453|1433D_SOYBN 14-3-3-like protein D (SGF14D) 297 2e-80
sp|P93212|1437_LYCES 14-3-3 protein 7 297 3e-80
sp|P42648|14331_ENTHI 14-3-3 protein 1 (14-3-3-1) 296 6e-80
sp|P34730|BMH2_YEAST Protein BMH2 296 6e-80
sp|P42649|14332_ENTHI 14-3-3 protein 2 (14-3-3-2) 296 6e-80
sp|P93208|14332_LYCES 14-3-3 protein 2 295 8e-80
>sp|P92177|1433E_DROME 14-3-3 protein epsilon (Suppressor of Ras1 3-9)
Length = 262
Score = 301 bits (770), Expect = 2e-81
Identities = 156/252 (61%), Positives = 199/252 (78%), Gaps = 1/252 (0%)
Frame = +2
Query: 17 MADREENVYRAKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASW 196
M +RE NVY+AKLAEQAERYDEMV++MK VA+ D EL+VEERNLLSVA+KNVIG+RRASW
Sbjct: 1 MTERENNVYKAKLAEQAERYDEMVEAMKKVASMDVELTVEERNLLSVAYKNVIGARRASW 60
Query: 197 RILSTIEQREEAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQV 376
RI+++IEQ+EE KG+ + ++E+ K YR QVEKEL ++C +++ VL+ L+P A++ ES+V
Sbjct: 61 RIITSIEQKEENKGAEE-KLEMIKTYRGQVEKELRDICSDILNVLEKHLIPCATSGESKV 119
Query: 377 FYLKMKGDYYRYRAEFAIEKEREEVAQKSLGAYESAMTEA-ESLSATHPIRLGLALNFSV 553
FY KMKGDY+RY AEFA +R++ A+ SL AY++A A L THPIRLGLALNFSV
Sbjct: 120 FYYKMKGDYHRYLAEFATGSDRKDAAENSLIAYKAASDIAMNDLPPTHPIRLGLALNFSV 179
Query: 554 FYFEIMGNPEKACSLAKTAFDQAIGELDNLTEEENYKDSTLIMQLLRDNLTLWNSGEEDD 733
FY+EI+ +P++AC LAK AFD AI ELD L+ EE+YKDSTLIMQLLRDNLTLW S D
Sbjct: 180 FYYEILNSPDRACRLAKAAFDDAIAELDTLS-EESYKDSTLIMQLLRDNLTLWTS----D 234
Query: 734 NKQNETNAEGGD 769
+ E + GD
Sbjct: 235 MQAEEVDPNAGD 246
>sp|P93214|14339_LYCES 14-3-3 protein 9
Length = 261
Score = 298 bits (762), Expect = 2e-80
Identities = 158/255 (61%), Positives = 197/255 (77%), Gaps = 5/255 (1%)
Frame = +2
Query: 23 DREENVYRAKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASWRI 202
+RE VY AKLAEQAERYDEMV++MK VA D EL+VEERNLLSV +KNV+GSRRASWRI
Sbjct: 6 ERENFVYVAKLAEQAERYDEMVEAMKNVANMDVELTVEERNLLSVGYKNVVGSRRASWRI 65
Query: 203 LSTIEQREEAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVFY 382
LS+IEQ+EE++G+ Q ++ K Y ++VE EL +C++++ V+D L+P S ES VFY
Sbjct: 66 LSSIEQKEESRGNEQ-NVKRIKEYLQKVESELTNICNDIMVVIDQHLIPSCSAGESTVFY 124
Query: 383 LKMKGDYYRYRAEFAIEKEREEVAQKSLGAYESAMTEAES-LSATHPIRLGLALNFSVFY 559
KMKGDYYRY AEF +++EVA+ SL AY++A T AE+ L+ THPIRLGLALNFSVFY
Sbjct: 125 HKMKGDYYRYLAEFKAGNDKKEVAELSLKAYQAATTAAEAELAPTHPIRLGLALNFSVFY 184
Query: 560 FEIMGNPEKACSLAKTAFDQAIGELDNLTEEENYKDSTLIMQLLRDNLTLWNS----GEE 727
+EIM +PE+AC LAK AFD+AI ELD+L E++YKDSTLIMQLLRDNLTLW S E
Sbjct: 185 YEIMNSPERACHLAKQAFDEAISELDSL-NEDSYKDSTLIMQLLRDNLTLWTSDLPEDAE 243
Query: 728 DDNKQNETNAEGGDQ 772
D K + TN GG +
Sbjct: 244 DAQKGDATNKAGGGE 258
>sp|Q9C5W6|143C_ARATH 14-3-3-like protein GF14 iota (General regulatory factor 12)
Length = 268
Score = 297 bits (761), Expect = 2e-80
Identities = 155/252 (61%), Positives = 199/252 (78%), Gaps = 6/252 (2%)
Frame = +2
Query: 23 DREENVYRAKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASWRI 202
+RE VY AKL+EQAERYDEMV++MK VA ++EL+VEERNLLSV +KNVIG+RRASWRI
Sbjct: 9 ERETFVYMAKLSEQAERYDEMVETMKKVARVNSELTVEERNLLSVGYKNVIGARRASWRI 68
Query: 203 LSTIEQREEAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVFY 382
+S+IEQ+EE+KG+ + ++ K YR++VE ELA +C +++ ++D L+P A++ E+ VFY
Sbjct: 69 MSSIEQKEESKGN-ESNVKQIKGYRQKVEDELANICQDILTIIDQHLIPHATSGEATVFY 127
Query: 383 LKMKGDYYRYRAEFAIEKEREEVAQKSLGAYESAMTEAES-LSATHPIRLGLALNFSVFY 559
KMKGDYYRY AEF E+ER+E A++SL YE+A A + L +THPIRLGLALNFSVFY
Sbjct: 128 YKMKGDYYRYLAEFKTEQERKEAAEQSLKGYEAATQAASTELPSTHPIRLGLALNFSVFY 187
Query: 560 FEIMGNPEKACSLAKTAFDQAIGELDNLTEEENYKDSTLIMQLLRDNLTLWNS-----GE 724
+EIM +PE+AC LAK AFD+AI ELD L+EE +YKDSTLIMQLLRDNLTLW S G
Sbjct: 188 YEIMNSPERACHLAKQAFDEAIAELDTLSEE-SYKDSTLIMQLLRDNLTLWTSDLPEDGG 246
Query: 725 EDDNKQNETNAE 760
ED+ K E+ E
Sbjct: 247 EDNIKTEESKQE 258
>sp|P48347|14310_ARATH 14-3-3-like protein GF14 epsilon (General regulatory factor 10)
Length = 254
Score = 297 bits (761), Expect = 2e-80
Identities = 153/251 (60%), Positives = 199/251 (79%), Gaps = 1/251 (0%)
Frame = +2
Query: 23 DREENVYRAKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASWRI 202
+RE+ VY AKL+EQ ERYDEMV++MK VA D EL+VEERNL+SV +KNVIG+RRASWRI
Sbjct: 4 EREKQVYLAKLSEQTERYDEMVEAMKKVAQLDVELTVEERNLVSVGYKNVIGARRASWRI 63
Query: 203 LSTIEQREEAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVFY 382
LS+IEQ+EE+KG+ + ++ K YRK+VE ELA+VC++++ V+D L+P ++ ES VF+
Sbjct: 64 LSSIEQKEESKGNDE-NVKRLKNYRKRVEDELAKVCNDILSVIDKHLIPSSNAVESTVFF 122
Query: 383 LKMKGDYYRYRAEFAIEKEREEVAQKSLGAYESAMTEAES-LSATHPIRLGLALNFSVFY 559
KMKGDYYRY AEF+ ER+E A +SL AY++A+ AE+ L+ THP+RLGLALNFSVFY
Sbjct: 123 YKMKGDYYRYLAEFSSGAERKEAADQSLEAYKAAVAAAENGLAPTHPVRLGLALNFSVFY 182
Query: 560 FEIMGNPEKACSLAKTAFDQAIGELDNLTEEENYKDSTLIMQLLRDNLTLWNSGEEDDNK 739
+EI+ +PE AC LAK AFD AI ELD+L EE+YKDSTLIMQLLRDNLTLW S D N+
Sbjct: 183 YEILNSPESACQLAKQAFDDAIAELDSL-NEESYKDSTLIMQLLRDNLTLWTS---DLNE 238
Query: 740 QNETNAEGGDQ 772
+ + +G D+
Sbjct: 239 EGDERTKGADE 249
>sp|Q96453|1433D_SOYBN 14-3-3-like protein D (SGF14D)
Length = 261
Score = 297 bits (761), Expect = 2e-80
Identities = 161/255 (63%), Positives = 195/255 (76%), Gaps = 5/255 (1%)
Frame = +2
Query: 23 DREENVYRAKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASWRI 202
DRE VY AKLAEQAERY+EMV+SMK VA D EL+VEERNLLSV +KNVIG+RRASWRI
Sbjct: 6 DRENFVYIAKLAEQAERYEEMVESMKNVANLDVELTVEERNLLSVGYKNVIGARRASWRI 65
Query: 203 LSTIEQREEAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVFY 382
LS+IEQ+EE KG +++ + K YR++VE EL+ +C++V++V+D L+P A+ ES VFY
Sbjct: 66 LSSIEQKEETKG-NELNAKRIKEYRQKVELELSNICNDVMRVIDEHLIPSAAAGESTVFY 124
Query: 383 LKMKGDYYRYRAEFAIEKEREEVAQKSLGAYESAMTEAES-LSATHPIRLGLALNFSVFY 559
KMKGDYYRY AEF E++E A +S+ AYESA AE+ L THPIRLGLALNFSVFY
Sbjct: 125 YKMKGDYYRYLAEFKSGNEKKEAADQSMKAYESATAAAEADLPPTHPIRLGLALNFSVFY 184
Query: 560 FEIMGNPEKACSLAKTAFDQAIGELDNLTEEENYKDSTLIMQLLRDNLTLWNS----GEE 727
+EI+ +PE+AC LAK AFD+AI ELD L EE+YKDSTLIMQLLRDNLTLW S E
Sbjct: 185 YEILNSPERACHLAKQAFDEAISELDTL-NEESYKDSTLIMQLLRDNLTLWTSDIPEDGE 243
Query: 728 DDNKQNETNAEGGDQ 772
D K N T GG +
Sbjct: 244 DAQKVNGTAKLGGGE 258
>sp|P93212|1437_LYCES 14-3-3 protein 7
Length = 252
Score = 297 bits (760), Expect = 3e-80
Identities = 150/250 (60%), Positives = 197/250 (78%), Gaps = 1/250 (0%)
Frame = +2
Query: 23 DREENVYRAKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASWRI 202
+RE+ VY A+LAEQAERYDEMV++MK +A D EL+VEERNL+SV +KNVIG+RRASWRI
Sbjct: 4 EREKQVYLARLAEQAERYDEMVEAMKAIAKMDVELTVEERNLVSVGYKNVIGARRASWRI 63
Query: 203 LSTIEQREEAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVFY 382
LS+IEQ+EE+KG Q ++ K YR++VE EL ++C +++ V+D L+P ++ ES VFY
Sbjct: 64 LSSIEQKEESKGHEQ-NVKRIKTYRQRVEDELTKICSDILSVIDEHLVPSSTTGESTVFY 122
Query: 383 LKMKGDYYRYRAEFAIEKEREEVAQKSLGAYESAMTEAES-LSATHPIRLGLALNFSVFY 559
KMKGDYYRY AEF +R+E +++SL AYE+A A S L+ THPIRLGLALNFSVFY
Sbjct: 123 YKMKGDYYRYLAEFKAGDDRKEASEQSLKAYEAATATASSDLAPTHPIRLGLALNFSVFY 182
Query: 560 FEIMGNPEKACSLAKTAFDQAIGELDNLTEEENYKDSTLIMQLLRDNLTLWNSGEEDDNK 739
+EI+ +PE+AC LAK AFD+AI ELD+L+ EE+YKDSTLIMQLLRDNLTLW S E+ +
Sbjct: 183 YEILNSPERACHLAKQAFDEAIAELDSLS-EESYKDSTLIMQLLRDNLTLWTSDLEEGGE 241
Query: 740 QNETNAEGGD 769
++ + G+
Sbjct: 242 HSKGDERQGE 251
>sp|P42648|14331_ENTHI 14-3-3 protein 1 (14-3-3-1)
Length = 239
Score = 296 bits (757), Expect = 6e-80
Identities = 154/241 (63%), Positives = 194/241 (80%), Gaps = 2/241 (0%)
Frame = +2
Query: 17 MADREENVYRAKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASW 196
MA RE+ VY AKLAEQ+ERYDEMV+ MK VA + ELS+EERNLLSVA+KNVIG++RASW
Sbjct: 1 MASREDCVYTAKLAEQSERYDEMVQCMKQVAEMEAELSIEERNLLSVAYKNVIGAKRASW 60
Query: 197 RILSTIEQREEAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQV 376
RI+S++EQ+E+AKG+ + +E+ K YR ++EKEL+ CD+V+KV+ LLPKAS +ES+V
Sbjct: 61 RIISSLEQKEQAKGNDK-HVEIIKGYRAKIEKELSTCCDDVLKVIQENLLPKASTSESKV 119
Query: 377 FYLKMKGDYYRYRAEFAIEKEREEVAQKSLGAYESA--MTEAESLSATHPIRLGLALNFS 550
F+ KM+GDYYRY AEF ++++R+EVA KSL AY A ++ AE L+ THPIRLGLALNFS
Sbjct: 120 FFKKMEGDYYRYFAEFTVDEKRKEVADKSLAAYTEATEISNAE-LAPTHPIRLGLALNFS 178
Query: 551 VFYFEIMGNPEKACSLAKTAFDQAIGELDNLTEEENYKDSTLIMQLLRDNLTLWNSGEED 730
VFYFEIM + +KAC LAK AFD AI +LD + E YKDSTLIMQLLRDNLTLW S D
Sbjct: 179 VFYFEIMNDADKACQLAKQAFDDAIAKLDEV-PENMYKDSTLIMQLLRDNLTLWTSDACD 237
Query: 731 D 733
+
Sbjct: 238 E 238
>sp|P34730|BMH2_YEAST Protein BMH2
Length = 273
Score = 296 bits (757), Expect = 6e-80
Identities = 154/255 (60%), Positives = 197/255 (77%), Gaps = 6/255 (2%)
Frame = +2
Query: 26 REENVYRAKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASWRIL 205
RE++VY AKLAEQAERY+EMV++MK VA+S ELSVEERNLLSVA+KNVIG+RRASWRI+
Sbjct: 5 REDSVYLAKLAEQAERYEEMVENMKAVASSGQELSVEERNLLSVAYKNVIGARRASWRIV 64
Query: 206 STIEQREEAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVFYL 385
S+IEQ+EE+K S+ ++EL ++YR ++E EL ++ D+++ VLD+ L+P A+ ES+VFY
Sbjct: 65 SSIEQKEESKEKSEHQVELIRSYRSKIETELTKISDDILSVLDSHLIPSATTGESKVFYY 124
Query: 386 KMKGDYYRYRAEFAIEKEREEVAQKSLGAYESAMTEAES-LSATHPIRLGLALNFSVFYF 562
KMKGDY+RY AEF+ RE+ SL AY++A A + L THPIRLGLALNFSVFY+
Sbjct: 125 KMKGDYHRYLAEFSSGDAREKATNSSLEAYKTASEIATTELPPTHPIRLGLALNFSVFYY 184
Query: 563 EIMGNPEKACSLAKTAFDQAIGELDNLTEEENYKDSTLIMQLLRDNLTLW-----NSGEE 727
EI +P+KAC LAK AFD AI ELD L+ EE+YKDSTLIMQLLRDNLTLW SG+E
Sbjct: 185 EIQNSPDKACHLAKQAFDDAIAELDTLS-EESYKDSTLIMQLLRDNLTLWTSDISESGQE 243
Query: 728 DDNKQNETNAEGGDQ 772
D +Q + + Q
Sbjct: 244 DQQQQQQQQQQQQQQ 258
>sp|P42649|14332_ENTHI 14-3-3 protein 2 (14-3-3-2)
Length = 238
Score = 296 bits (757), Expect = 6e-80
Identities = 149/239 (62%), Positives = 195/239 (81%)
Frame = +2
Query: 17 MADREENVYRAKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASW 196
M RE+ VY +KLAEQ+ERY+EMV+ MK VA TELSVEERNL+SVA+KNV+GSRRASW
Sbjct: 1 MTSREDLVYLSKLAEQSERYEEMVQYMKQVAEMGTELSVEERNLISVAYKNVVGSRRASW 60
Query: 197 RILSTIEQREEAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQV 376
RI+S++EQ+E+AKG++Q R+EL K YR ++E+EL++ CD+V+K++ LL +++ ES+V
Sbjct: 61 RIISSLEQKEQAKGNTQ-RVELIKTYRAKIEQELSQKCDDVLKIITEFLLKNSTSIESKV 119
Query: 377 FYLKMKGDYYRYRAEFAIEKEREEVAQKSLGAYESAMTEAESLSATHPIRLGLALNFSVF 556
F+ KM+GDYYRY AEF ++++R+EVA KSL AY+ A A SL THPIRLGLALNFSVF
Sbjct: 120 FFKKMEGDYYRYYAEFTVDEKRKEVADKSLAAYQEATDTAASLVPTHPIRLGLALNFSVF 179
Query: 557 YFEIMGNPEKACSLAKTAFDQAIGELDNLTEEENYKDSTLIMQLLRDNLTLWNSGEEDD 733
Y++IM + +KAC LAK AFD+AI +LD + EE+YK+STLIMQLLRDNLTLW S DD
Sbjct: 180 YYQIMNDADKACQLAKEAFDEAIQKLDEV-PEESYKESTLIMQLLRDNLTLWTSDMGDD 237
>sp|P93208|14332_LYCES 14-3-3 protein 2
Length = 254
Score = 295 bits (756), Expect = 8e-80
Identities = 151/239 (63%), Positives = 196/239 (82%), Gaps = 3/239 (1%)
Frame = +2
Query: 26 REENVYRAKLAEQAERYDEMVKSMKCVAAS--DTELSVEERNLLSVAFKNVIGSRRASWR 199
REENVY AKLAEQAERY+EMV+ M+ V+ S EL+VEERNLLSVA+KNVIG+RRASWR
Sbjct: 3 REENVYMAKLAEQAERYEEMVQFMEKVSTSLGSEELTVEERNLLSVAYKNVIGARRASWR 62
Query: 200 ILSTIEQREEAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVF 379
I+S+IEQ+EE++G+ + ++ K YR ++E EL+++CD ++K+LD+ L+P ASN +S+VF
Sbjct: 63 IISSIEQKEESRGNEE-HVKCIKEYRSKIESELSDICDGILKLLDSNLIPSASNGDSKVF 121
Query: 380 YLKMKGDYYRYRAEFAIEKEREEVAQKSLGAYESAMTEAES-LSATHPIRLGLALNFSVF 556
YLKMKGDY+RY AEF ER+E A+ +L AY++A A + L+ THPIRLGLALNFSVF
Sbjct: 122 YLKMKGDYHRYLAEFKTGAERKEAAESTLSAYKAAQDIANTELAPTHPIRLGLALNFSVF 181
Query: 557 YFEIMGNPEKACSLAKTAFDQAIGELDNLTEEENYKDSTLIMQLLRDNLTLWNSGEEDD 733
Y+EI+ +P++AC+LAK AFD+AI ELD L EE+YKDSTLIMQLLRDNLTLW S +DD
Sbjct: 182 YYEILNSPDRACNLAKQAFDEAIAELDTL-GEESYKDSTLIMQLLRDNLTLWTSDMQDD 239
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,184,884
Number of Sequences: 369166
Number of extensions: 1524543
Number of successful extensions: 5413
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4905
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5059
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8341863645
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)