Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_F18
(867 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 134 2e-31
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 133 7e-31
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 130 6e-30
sp|P27636|CDC15_YEAST Cell division control protein 15 129 1e-29
sp|P41892|CDC7_SCHPO Cell division control protein 7 124 3e-28
sp|Q01389|BCK1_YEAST Serine/threonine-protein kinase BCK1/S... 123 6e-28
sp|Q9Y6R4|M3K4_HUMAN Mitogen-activated protein kinase kinas... 122 1e-27
sp|O08648|M3K4_MOUSE Mitogen-activated protein kinase kinas... 121 2e-27
sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinas... 120 6e-27
sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinas... 119 1e-26
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 134 bits (338), Expect = 2e-31
Identities = 89/258 (34%), Positives = 137/258 (53%), Gaps = 7/258 (2%)
Frame = +3
Query: 6 FGSVYRCSKFGSKDMVVLKKY---KNPNDKDE---ELKKLMVEFSIYEKLGDHPRILKCN 167
FG+VY S +++ +K+ N K++ +++L E + + L HP I++
Sbjct: 79 FGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHIQELEEEVKLLKNLS-HPNIVRYL 137
Query: 168 GFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDL 347
G R + I L+++ GS+ S L K GA E + ++ Q+L L++LH+ I+HRD+
Sbjct: 138 GTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRTYTNQLLLGLEYLHNHAIMHRDI 197
Query: 348 KGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSS 527
KG IL+DN IKL F ++K + ++ + A+S GT W APEVI G+ S
Sbjct: 198 KGANILVDNQGCIKLADFGASKQVAELATIS----GAKSMKGTPYWMAPEVILQTGHSFS 253
Query: 528 ADIWSFGAVMVELITGKAPLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLD 704
ADIWS G ++E++TGKAP AI+ +GT K P S F+ CL
Sbjct: 254 ADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKS-HPPIPDNISSDANDFLLKCLQ 312
Query: 705 PEPLNRPTVKDLMKHEFL 758
EP RPT +L+KH F+
Sbjct: 313 QEPNLRPTASELLKHPFV 330
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 133 bits (334), Expect = 7e-31
Identities = 88/258 (34%), Positives = 135/258 (52%), Gaps = 7/258 (2%)
Frame = +3
Query: 6 FGSVYRCSKFGSKDMVVLKK------YKNPNDKDEELKKLMVEFSIYEKLGDHPRILKCN 167
FG+VY S +++ +K+ + + +++L E + + L HP I++
Sbjct: 80 FGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQELEEEVKLLKNLS-HPNIVRYL 138
Query: 168 GFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDL 347
G R I L+++ GS+ S L K G E + +++Q+L L++LH+ I+HRD+
Sbjct: 139 GTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYTRQLLLGLEYLHNHAIMHRDI 198
Query: 348 KGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSS 527
KG IL+DN IKL F ++K + ++ T A+S GT W APEVI G+ S
Sbjct: 199 KGANILVDNKGCIKLADFGASKQVAELATMT----GAKSMKGTPYWMAPEVILQTGHSFS 254
Query: 528 ADIWSFGAVMVELITGKAPLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLD 704
ADIWS G ++E++TGKAP AI+ +GT K SDA K F+ CL
Sbjct: 255 ADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPDTLSSDA-KDFLLKCLQ 313
Query: 705 PEPLNRPTVKDLMKHEFL 758
P RPT +L+KH F+
Sbjct: 314 EVPNLRPTASELLKHPFV 331
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 130 bits (326), Expect = 6e-30
Identities = 86/258 (33%), Positives = 137/258 (53%), Gaps = 7/258 (2%)
Frame = +3
Query: 6 FGSVYRCSKFGSKDMVVLKKYK-NPNDKDEE-----LKKLMVEFSIYEKLGDHPRILKCN 167
FG VY S +++ +K+ P+ +E +++L E + + L HP I++
Sbjct: 79 FGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIRELEEEVQLLKNLS-HPNIVRYL 137
Query: 168 GFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDL 347
G R + I ++++ GS+ S L K G+ EP II ++KQ+L L++LH+ I+HRD+
Sbjct: 138 GTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYTKQLLLGLEYLHNNGIMHRDI 197
Query: 348 KGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSS 527
KG IL+DN I+L F ++K + ++ + A+S GT W APEVI G+ S
Sbjct: 198 KGANILVDNKGCIRLADFGASKKVVELATV----NGAKSMKGTPYWMAPEVILQTGHSFS 253
Query: 528 ADIWSFGAVMVELITGKAPLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLD 704
ADIWS G ++E+ TGK P F A+ +G + K P S K F+ CL
Sbjct: 254 ADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIG-RTKAHPPIPEDLSPEAKDFLMKCLH 312
Query: 705 PEPLNRPTVKDLMKHEFL 758
EP R + +L++H F+
Sbjct: 313 KEPSLRLSATELLQHPFV 330
>sp|P27636|CDC15_YEAST Cell division control protein 15
Length = 974
Score = 129 bits (324), Expect = 1e-29
Identities = 83/254 (32%), Positives = 123/254 (48%), Gaps = 1/254 (0%)
Frame = +3
Query: 6 FGSVYRCSKFGSKDMVVLKKYKNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSV 185
+G VY+ + +V +K+ ND EEL +M E S+ + L +H I+K +GF R
Sbjct: 36 YGVVYKAINKHTDQVVAIKEVVYEND--EELNDIMAEISLLKNL-NHNNIVKYHGFIRKS 92
Query: 186 KASYIKLDYMANGSLRSWLHKNGAQ-KEPSIIRFSKQILEALDFLHSQNILHRDLKGPKI 362
YI L+Y ANGSLR + ++ E + Q L L +LH + ++HRD+K I
Sbjct: 93 YELYILLEYCANGSLRRLISRSSTGLSENESKTYVTQTLLGLKYLHGEGVIHRDIKAANI 152
Query: 363 LLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWS 542
LL + +KL F T N A + GT NW APE++ G + +DIWS
Sbjct: 153 LLSADNTVKLADF---------GVSTIVNSSALTLAGTLNWMAPEILGNRGASTLSDIWS 203
Query: 543 FGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNR 722
GA +VE++T P L Y + + + P S+ LK F+ C R
Sbjct: 204 LGATVVEMLTKNPPYHNLTDANIYYAV---ENDTYYPPSSFSEPLKDFLSKCFVKNMYKR 260
Query: 723 PTVKDLMKHEFLNS 764
PT L+KH ++NS
Sbjct: 261 PTADQLLKHVWINS 274
>sp|P41892|CDC7_SCHPO Cell division control protein 7
Length = 1062
Score = 124 bits (311), Expect = 3e-28
Identities = 82/251 (32%), Positives = 126/251 (50%)
Frame = +3
Query: 6 FGSVYRCSKFGSKDMVVLKKYKNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSV 185
FG+VYR + + V +KK K +L + +E + + L DHP I+K G +++
Sbjct: 20 FGAVYRGLNIKNGETVAVKKVKLSKMLKSDLSVIKMEIDLLKNL-DHPNIVKYRGSYQTN 78
Query: 186 KASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKIL 365
+ I L+Y NGSLRS G E + ++ Q+L+ L +LH+Q ++HRD+KG IL
Sbjct: 79 DSLCIILEYCENGSLRSICKNFGKIPENLVALYTFQVLQGLLYLHNQGVIHRDIKGANIL 138
Query: 366 LDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSF 545
+ IKL F A +H S G+ W APEVI G +++DIWS
Sbjct: 139 TTKDGTIKLADFGVA-----TKINALEDH---SVVGSPYWMAPEVIELVGATTASDIWSV 190
Query: 546 GAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRP 725
G ++EL+ G P + L A++R+ K P S A KSF+ C +P R
Sbjct: 191 GCTVIELLDGNPPYYDLDPTSALFRM--VKDEHPPLPSNISSAAKSFLMQCFQKDPNLRI 248
Query: 726 TVKDLMKHEFL 758
+ L+KH ++
Sbjct: 249 KTRKLLKHPWV 259
>sp|Q01389|BCK1_YEAST Serine/threonine-protein kinase BCK1/SLK1/SSP31
Length = 1478
Score = 123 bits (309), Expect = 6e-28
Identities = 81/261 (31%), Positives = 131/261 (50%), Gaps = 11/261 (4%)
Frame = +3
Query: 6 FGSVYRCSKFGSKDMVVLKKYKNPNDKDEE------LKKLMVEFSIYEKLGDHPRILKCN 167
FG+VY C + +M+ +K+ + P + ++ L E S + L DH I++
Sbjct: 1186 FGAVYLCLNVTTGEMMAVKQVEVPKYSSQNEAILSTVEALRSEVSTLKDL-DHLNIVQYL 1244
Query: 168 GFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDL 347
GF + L+Y+A GS+ S + G EP I + Q+L+ L +LHS+ ILHRD+
Sbjct: 1245 GFENKNNIYSLFLEYVAGGSVGSLIRMYGRFDEPLIKHLTTQVLKGLAYLHSKGILHRDM 1304
Query: 348 KGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVIN-GEGYGS 524
K +LLD + K+ F ++ D S + + GT W APE+++ +GY +
Sbjct: 1305 KADNLLLDQDGICKISDFGISRKSKDIYSNS-----DMTMRGTVFWMAPEMVDTKQGYSA 1359
Query: 525 SADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKI----EFAYPLKCSDALKSFVD 692
DIWS G +++E+ GK P L A++++G K E PL S ++F+D
Sbjct: 1360 KVDIWSLGCIVLEMFAGKRPWSNLEVVAAMFKIGKSKSAPPIPEDTLPL-ISQIGRNFLD 1418
Query: 693 PCLDPEPLNRPTVKDLMKHEF 755
C + P RPT +L+ H F
Sbjct: 1419 ACFEINPEKRPTANELLSHPF 1439
>sp|Q9Y6R4|M3K4_HUMAN Mitogen-activated protein kinase kinase kinase 4 (MAPK/ERK kinase
kinase 4) (MEK kinase 4) (MEKK 4) (MAP three kinase 1)
Length = 1607
Score = 122 bits (307), Expect = 1e-27
Identities = 90/258 (34%), Positives = 142/258 (55%), Gaps = 7/258 (2%)
Frame = +3
Query: 6 FGSVYRCSKFGSKDMVVLKKYK-NPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRS 182
+G VY C + +++ +K+ + PND + +K+ E I+E + HP +++ G
Sbjct: 1353 YGKVYTCISVDTGELMAMKEIRFQPNDH-KTIKETADELKIFEGI-KHPNLVRYFGVELH 1410
Query: 183 VKASYIKLDYMANGSLRSWLHKNGAQKEPSIIR-FSKQILEALDFLHSQNILHRDLKGPK 359
+ YI ++Y G+L + + G Q+ +IR +SKQI A++ LH I+HRD+KG
Sbjct: 1411 REEMYIFMEYCDEGTLEE-VSRLGLQEH--VIRLYSKQITIAINVLHEHGIVHRDIKGAN 1467
Query: 360 ILLDNNLDIKLCGF-MSAKMFPDASSETFRNHVAESNHGTANWTAPEVIN---GEGYGSS 527
I L ++ IKL F S K+ +A +T V S GTA + APEVI GEG+G +
Sbjct: 1468 IFLTSSGLIKLGDFGCSVKLKNNA--QTMPGEV-NSTLGTAAYMAPEVITRAKGEGHGRA 1524
Query: 528 ADIWSFGAVMVELITGKAPLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLD 704
ADIWS G V++E++TGK P + +FQ +Y++G K P + S K F+ CL+
Sbjct: 1525 ADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPI--PERLSPEGKDFLSHCLE 1582
Query: 705 PEPLNRPTVKDLMKHEFL 758
+P R T L+ H F+
Sbjct: 1583 SDPKMRWTASQLLDHSFV 1600
>sp|O08648|M3K4_MOUSE Mitogen-activated protein kinase kinase kinase 4 (MAPK/ERK kinase
kinase 4) (MEK kinase 4) (MEKK 4)
Length = 1597
Score = 121 bits (304), Expect = 2e-27
Identities = 89/258 (34%), Positives = 143/258 (55%), Gaps = 7/258 (2%)
Frame = +3
Query: 6 FGSVYRCSKFGSKDMVVLKKYK-NPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRS 182
+G VY C + +++ +K+ + PND + +K+ E I+E + HP +++ G
Sbjct: 1343 YGKVYTCISVDTGELMAMKEIRFQPNDH-KTIKETADELKIFEGI-KHPNLVRYFGVELH 1400
Query: 183 VKASYIKLDYMANGSLRSWLHKNGAQKEPSIIR-FSKQILEALDFLHSQNILHRDLKGPK 359
+ YI ++Y G+L + + G Q+ +IR ++KQI A++ LH I+HRD+KG
Sbjct: 1401 REEMYIFMEYCDEGTLEE-VSRLGLQEH--VIRLYTKQITVAINVLHEHGIVHRDIKGAN 1457
Query: 360 ILLDNNLDIKLCGF-MSAKMFPDASSETFRNHVAESNHGTANWTAPEVIN---GEGYGSS 527
I L ++ IKL F S K+ +A +T V S GTA + APEVI GEG+G +
Sbjct: 1458 IFLTSSGLIKLGDFGCSVKLKNNA--QTMPGEV-NSTLGTAAYMAPEVITRAKGEGHGRA 1514
Query: 528 ADIWSFGAVMVELITGKAPLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLD 704
ADIWS G V++E++TGK P + +FQ +Y++G K P + S K+F+ CL+
Sbjct: 1515 ADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPI--PERLSPEGKAFLSHCLE 1572
Query: 705 PEPLNRPTVKDLMKHEFL 758
+P R T L+ H F+
Sbjct: 1573 SDPKIRWTASQLLDHAFV 1590
>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 120 bits (300), Expect = 6e-27
Identities = 85/256 (33%), Positives = 130/256 (50%), Gaps = 6/256 (2%)
Frame = +3
Query: 6 FGSVYRCSKFGSKDMVVLKKYKNPNDKDE---ELKKLMVEFSIYEKLGDHPRILKCNGFF 176
FG VY C + + K+ + D E E+ L E + + L H RI++ G
Sbjct: 373 FGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLKNL-QHERIVQYYGCL 431
Query: 177 R--SVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLK 350
R + K I ++YM GS++ L GA E ++++QILE + +LHS I+HRD+K
Sbjct: 432 RDRAEKTLTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIK 491
Query: 351 GPKILLDNNLDIKLCGFMSAKMFPD-ASSETFRNHVAESNHGTANWTAPEVINGEGYGSS 527
G IL D+ ++KL F ++K S T V GT W +PEVI+GEGYG
Sbjct: 492 GANILRDSAGNVKLGDFGASKRLQTICMSGTGMRSVT----GTPYWMSPEVISGEGYGRK 547
Query: 528 ADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDP 707
AD+WS G +VE++T K P + AI+++ TQ P S+ + F+ +
Sbjct: 548 ADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQ-PTNPQLPSHISEHGRDFLRR-IFV 605
Query: 708 EPLNRPTVKDLMKHEF 755
E RP+ ++L+ H F
Sbjct: 606 EARQRPSAEELLTHHF 621
>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 119 bits (298), Expect = 1e-26
Identities = 83/255 (32%), Positives = 129/255 (50%), Gaps = 5/255 (1%)
Frame = +3
Query: 6 FGSVYRCSKFGSKDMVVLKKYKNPNDKDE---ELKKLMVEFSIYEKLGDHPRILKCNGFF 176
FG VY C + + K+ + D E E+ L E + + L H RI++ G
Sbjct: 373 FGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLKNL-QHERIVQYYGCL 431
Query: 177 R--SVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLK 350
R + K I ++YM GS++ L GA E ++++QILE + +LHS I+HRD+K
Sbjct: 432 RDRAEKILTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIK 491
Query: 351 GPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSA 530
G IL D+ ++KL F ++K + S GT W +PEVI+GEGYG A
Sbjct: 492 GANILRDSAGNVKLGDFGASKRL---QTICMSGTGIRSVTGTPYWMSPEVISGEGYGRKA 548
Query: 531 DIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPE 710
D+WS G +VE++T K P + AI+++ TQ P S+ + F+ + E
Sbjct: 549 DVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQ-PTNPQLPSHISEHGRDFLRR-IFVE 606
Query: 711 PLNRPTVKDLMKHEF 755
RP+ ++L+ H F
Sbjct: 607 ARQRPSAEELLTHHF 621
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 104,968,084
Number of Sequences: 369166
Number of extensions: 2220738
Number of successful extensions: 9029
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6940
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7381
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8550075140
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)