Planarian EST Database


Dr_sW_011_E17

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_011_E17
         (894 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P43394|K502_ACTCH  Fruit protein PKIWI502                       32   1.8  
sp|P16065|GCY_STRPU  Speract receptor precursor (Guanylate c...    31   5.3  
sp|Q6KIH8|GRPE_MYCMO  Protein grpE (HSP-70 cofactor)               31   5.3  
sp|P17276|PH4H_DROME  Protein henna [Includes: Phenylalanine...    30   7.0  
sp|O52354|LDH_MYCGA  L-lactate dehydrogenase (L-LDH)               30   7.0  
>sp|P43394|K502_ACTCH Fruit protein PKIWI502
          Length = 317

 Score = 32.3 bits (72), Expect = 1.8
 Identities = 20/63 (31%), Positives = 31/63 (49%)
 Frame = +2

Query: 38  LSQEAVI*LHRHLQAAPMHYRPQTVLEIPQIYHHQHR*IWCILSILNLSDFLIAIYESKI 217
           LS+   + LH  L  AP H+RP   L  P + +H H  +  + S + L D   AI +   
Sbjct: 15  LSRHPSLTLHSSLSHAPPHHRPVAFLRHPTLRYHHHGRLLSVASAI-LQD--TAIRQDTY 71

Query: 218 MWS 226
           +W+
Sbjct: 72  IWT 74
>sp|P16065|GCY_STRPU Speract receptor precursor (Guanylate cyclase)
          Length = 1125

 Score = 30.8 bits (68), Expect = 5.3
 Identities = 18/70 (25%), Positives = 32/70 (45%)
 Frame = +1

Query: 397 MRGMADFSSHYVYNLNTQVFIEKQSDNKFLNTVNIRHVANSVRTHGWGIINTTINNAYVF 576
           +R   DF+S Y+++   Q+          L  ++  H A      G  +++T +N+ Y  
Sbjct: 365 LRAEIDFASVYMFDATMQL----------LEALDRTHAAGGDIYDGEEVVSTLLNSTYRS 414

Query: 577 LKDKFFQFSE 606
             D F+QF E
Sbjct: 415 KTDTFYQFDE 424
>sp|Q6KIH8|GRPE_MYCMO Protein grpE (HSP-70 cofactor)
          Length = 243

 Score = 30.8 bits (68), Expect = 5.3
 Identities = 11/38 (28%), Positives = 26/38 (68%)
 Frame = +1

Query: 592 FQFSEFLYDERIKGKLIKELHEFKERSATTDKSFSYYN 705
           ++FS+F +D+++ GK ++   + ++R A++DK+    N
Sbjct: 5   YKFSDFYFDKKLSGKEVEFSIQIQKREASSDKNLQSKN 42
>sp|P17276|PH4H_DROME Protein henna [Includes: Phenylalanine-4-hydroxylase (PAH)
           (Phe-4-monooxygenase); Tryptophan 5-monooxygenase (TRH)
           (Tryptophan 5-hydroxylase)]
          Length = 452

 Score = 30.4 bits (67), Expect = 7.0
 Identities = 32/137 (23%), Positives = 57/137 (41%), Gaps = 6/137 (4%)
 Frame = +1

Query: 214 DYVEHHLEN---VFYNLTAIYLSDWRNYAEMRRLAQNQYN--ICLTTPDMHLPSQSLEQG 378
           DY +  +E    +F NLT +Y +           A  +YN    L   +      ++ Q 
Sbjct: 177 DYTKEEIETWGIIFRNLTKLYKTH----------ACREYNHVFPLLVDNCGFREDNIPQL 226

Query: 379 RDVLNIMRGMADFSSHYVYNL-NTQVFIEKQSDNKFLNTVNIRHVANSVRTHGWGIINTT 555
            DV N +R    F+   V  L +++ F+   +   F +T  IRH +  + T    + +  
Sbjct: 227 EDVSNFLRDCTGFTLRPVAGLLSSRDFLAGLAFRVFHSTQYIRHPSKPMYTPEPDVCHEL 286

Query: 556 INNAYVFLKDKFFQFSE 606
           + +  +F    F QFS+
Sbjct: 287 MGHVPLFADPAFAQFSQ 303
>sp|O52354|LDH_MYCGA L-lactate dehydrogenase (L-LDH)
          Length = 323

 Score = 30.4 bits (67), Expect = 7.0
 Identities = 16/48 (33%), Positives = 25/48 (52%)
 Frame = +1

Query: 394 IMRGMADFSSHYVYNLNTQVFIEKQSDNKFLNTVNIRHVANSVRTHGW 537
           I   M+  +  Y+YNLNT + +  Q D+K++ +     V   V  HGW
Sbjct: 238 IAESMSLITRAYIYNLNTVLGVGVQLDDKYVASGIYFTVPAVVGKHGW 285
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 101,729,546
Number of Sequences: 369166
Number of extensions: 1997729
Number of successful extensions: 5311
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 5052
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5301
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8982382310
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)