Planarian EST Database


Dr_sW_011_E11

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_011_E11
         (900 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q13435|SF3B2_HUMAN  Splicing factor 3B subunit 2 (Spliceo...   314   2e-85
sp|Q02554|CUS1_YEAST  Protein CUS1                                119   2e-26
sp|Q5R789|ZCHC8_PONPY  Zinc finger CCHC domain containing pr...    41   0.005
sp|Q9CYA6|ZCHC8_MOUSE  Zinc finger CCHC domain containing pr...    41   0.005
sp|Q6NZY4|ZCHC8_HUMAN  Zinc finger CCHC domain containing pr...    41   0.005
sp|Q5F3D1|ZCHC8_CHICK  Zinc finger CCHC domain containing pr...    38   0.044
sp|P30415|NKTR_MOUSE  NK-tumor recognition protein (Natural-...    33   1.1  
sp|Q9NRM1|ENAM_HUMAN  Enamelin precursor                           32   1.9  
sp|Q9FFK1|SY31_ARATH  Syntaxin 31 (AtSYP31) (AtSED5)               32   2.4  
sp|Q9GKL2|STF1_HORSE  Steroidogenic factor 1 (STF-1) (SF-1)        32   2.4  
>sp|Q13435|SF3B2_HUMAN Splicing factor 3B subunit 2 (Spliceosome associated protein 145)
            (SAP 145) (SF3b150) (Pre-mRNA splicing factor SF3b 145
            kDa subunit)
          Length = 872

 Score =  314 bits (804), Expect = 2e-85
 Identities = 142/220 (64%), Positives = 172/220 (78%)
 Frame = +3

Query: 9    RNTVPVPRHWCCTGKYLQGKRGFVKPPFKLPDFIAKTGIMEMRQTADDKESEKSLKNKMK 188
            RN+VPVPRHWC   KYLQGKRG  KPPF+LPDFI +TGI EMR+   +KE +K++K+KM+
Sbjct: 472  RNSVPVPRHWCFKRKYLQGKRGIEKPPFELPDFIKRTGIQEMREALQEKEEQKTMKSKMR 531

Query: 189  DKIRPKVGLVDISYEKLYNAFFVEQTKPELTKFGDIYYQGKELEAKRKENRPGLLSDELR 368
            +K+RPK+G +DI Y+KL++AFF  QTKP+LT  GD+YY+GKE E + KE +PG LSDELR
Sbjct: 532  EKVRPKMGKIDIDYQKLHDAFFKWQTKPKLTIHGDLYYEGKEFETRLKEKKPGDLSDELR 591

Query: 369  VALGMTTGPNSDKSPPPWLIAQQRYGPPPSYPNLKIPGLNKGIPPGCSFGFHAGGWGKPP 548
            ++LGM  GPN+ K PPPWLIA QRYGPPPSYPNLKIPGLN  IP  CSFG+HAGGWGKPP
Sbjct: 592  ISLGMPVGPNAHKVPPPWLIAMQRYGPPPSYPNLKIPGLNSPIPESCSFGYHAGGWGKPP 651

Query: 549  VDDDGKPLYGDVFANAFNTAPVLIDLDADEDDEKYWGEVE 668
            VD+ GKPLYGDVF    N A      + +E D   WGE+E
Sbjct: 652  VDETGKPLYGDVFGT--NAAEFQTKTEEEEIDRTPWGELE 689

 Score = 30.8 bits (68), Expect = 5.4
 Identities = 23/68 (33%), Positives = 29/68 (42%), Gaps = 7/68 (10%)
 Frame = +3

Query: 390 GPNSDKSPPPWLIAQ----QRYGPPPSYPNLKIPGLNKGIPPGCSFGF---HAGGWGKPP 548
           G + DK+ PP + AQ        PP   P L+ P      PPG   GF   H    G PP
Sbjct: 49  GEDGDKAAPPPMSAQLPGIPMPPPPLGLPPLQPPPPPPPPPPGLGLGFPMAHPPNLGPPP 108

Query: 549 VDDDGKPL 572
               G+P+
Sbjct: 109 PLRVGEPV 116
>sp|Q02554|CUS1_YEAST Protein CUS1
          Length = 436

 Score =  119 bits (297), Expect = 2e-26
 Identities = 67/173 (38%), Positives = 95/173 (54%), Gaps = 13/173 (7%)
 Frame = +3

Query: 9   RNTVPVPRHWCCTGKYLQGKRGFVKPPFKLPDFIAKTGIMEMRQTAD----DKESEKSLK 176
           +N +PVP HW    +YL G+    K PF+LPD I KT I +MR T      D + EKSLK
Sbjct: 167 KNVIPVPSHWQSKKEYLSGRSLLGKRPFELPDIIKKTNIEQMRSTLPQSGLDGQDEKSLK 226

Query: 177 NKMKDKIRPKVGLVDISYEKLYNAFFV--EQTKPE-LTKFGDIYYQGKELEAKRK----- 332
              + +++PK+G +D+ Y+KL++ FF      KP+ L  FGD+YY+ + L  +       
Sbjct: 227 EASRARVQPKMGALDLDYKKLHDVFFKIGANWKPDHLLCFGDVYYENRNLFEETNWKRMV 286

Query: 333 -ENRPGLLSDELRVALGMTTGPNSDKSPPPWLIAQQRYGPPPSYPNLKIPGLN 488
              RPG +S ELR  + +  G       PPW +  +  G P  YP+LKI GLN
Sbjct: 287 DHKRPGRISQELRAIMNLPEG-----QLPPWCMKMKDIGLPTGYPDLKIAGLN 334
>sp|Q5R789|ZCHC8_PONPY Zinc finger CCHC domain containing protein 8
          Length = 704

 Score = 40.8 bits (94), Expect = 0.005
 Identities = 20/54 (37%), Positives = 34/54 (62%)
 Frame = +3

Query: 300 YQGKELEAKRKENRPGLLSDELRVALGMTTGPNSDKSPPPWLIAQQRYGPPPSY 461
           Y  +E+E +    +PG++S+EL+ ALG+T     DKS PP++   ++ G PP +
Sbjct: 272 YHAEEVEERFGRFKPGVISEELQDALGVT-----DKSLPPFIYRMRQLGYPPGW 320
>sp|Q9CYA6|ZCHC8_MOUSE Zinc finger CCHC domain containing protein 8
          Length = 709

 Score = 40.8 bits (94), Expect = 0.005
 Identities = 20/54 (37%), Positives = 34/54 (62%)
 Frame = +3

Query: 300 YQGKELEAKRKENRPGLLSDELRVALGMTTGPNSDKSPPPWLIAQQRYGPPPSY 461
           Y  +E+E +    +PG++S+EL+ ALG+T     DKS PP++   ++ G PP +
Sbjct: 277 YHAEEVEERFGRFKPGVISEELQDALGVT-----DKSLPPFIYRMRQLGYPPGW 325
>sp|Q6NZY4|ZCHC8_HUMAN Zinc finger CCHC domain containing protein 8
          Length = 707

 Score = 40.8 bits (94), Expect = 0.005
 Identities = 20/54 (37%), Positives = 34/54 (62%)
 Frame = +3

Query: 300 YQGKELEAKRKENRPGLLSDELRVALGMTTGPNSDKSPPPWLIAQQRYGPPPSY 461
           Y  +E+E +    +PG++S+EL+ ALG+T     DKS PP++   ++ G PP +
Sbjct: 274 YHAEEVEERFGRFKPGVISEELQDALGVT-----DKSLPPFIYRMRQLGYPPGW 322
>sp|Q5F3D1|ZCHC8_CHICK Zinc finger CCHC domain containing protein 8
          Length = 613

 Score = 37.7 bits (86), Expect = 0.044
 Identities = 19/54 (35%), Positives = 33/54 (61%)
 Frame = +3

Query: 300 YQGKELEAKRKENRPGLLSDELRVALGMTTGPNSDKSPPPWLIAQQRYGPPPSY 461
           Y  +E+E +  + +PG++S EL+ ALG+T      KS PP++   ++ G PP +
Sbjct: 173 YHAEEVEERFGKFKPGVISGELQDALGVTA-----KSLPPFIYRMRQLGYPPGW 221
>sp|P30415|NKTR_MOUSE NK-tumor recognition protein (Natural-killer cells
            cyclophilin-related protein) (NK-TR protein)
          Length = 1453

 Score = 33.1 bits (74), Expect = 1.1
 Identities = 24/101 (23%), Positives = 46/101 (45%), Gaps = 1/101 (0%)
 Frame = +3

Query: 111  AKTGIMEMRQTADDKESEKSLKNKMKDKIRPKVGLVDISYEKLYNAFFVEQTKPELTKFG 290
            + T +    +T+  KE  +  +  + DK +P  G+ ++S      +  ++     L+   
Sbjct: 1189 SSTSVTSPVETSGKKEGAEKSQMNLTDKWKPLQGVGNLSVSTATTSSALDVKA--LSTVP 1246

Query: 291  DIYYQGKELEAKRKEN-RPGLLSDELRVALGMTTGPNSDKS 410
            ++  QG  +E K K   RPG L DE+R    +   P + +S
Sbjct: 1247 EVKPQGLRIEIKSKNKVRPGSLFDEVRKTARLNRRPRNQES 1287
>sp|Q9NRM1|ENAM_HUMAN Enamelin precursor
          Length = 1142

 Score = 32.3 bits (72), Expect = 1.9
 Identities = 19/45 (42%), Positives = 22/45 (48%)
 Frame = +3

Query: 414 PPWLIAQQRYGPPPSYPNLKIPGLNKGIPPGCSFGFHAGGWGKPP 548
           PPW I Q+   PPP Y    I     G P    FG+H  G G+PP
Sbjct: 172 PPWQIPQRL--PPPGYGRPPISNEEGGNPYFGYFGYHGFG-GRPP 213
>sp|Q9FFK1|SY31_ARATH Syntaxin 31 (AtSYP31) (AtSED5)
          Length = 336

 Score = 32.0 bits (71), Expect = 2.4
 Identities = 20/61 (32%), Positives = 25/61 (40%)
 Frame = +3

Query: 324 KRKENRPGLLSDELRVALGMTTGPNSDKSPPPWLIAQQRYGPPPSYPNLKIPGLNKGIPP 503
           K  ENR  L S +  V         S   PPPW  +   +G   +     +P LN G PP
Sbjct: 157 KAHENRKQLFSTKNAVDSPPQNNAKSVPEPPPWSSSSNPFG---NLQQPLLPPLNTGAPP 213

Query: 504 G 506
           G
Sbjct: 214 G 214
>sp|Q9GKL2|STF1_HORSE Steroidogenic factor 1 (STF-1) (SF-1)
          Length = 461

 Score = 32.0 bits (71), Expect = 2.4
 Identities = 29/97 (29%), Positives = 42/97 (43%), Gaps = 1/97 (1%)
 Frame = +3

Query: 282 KFGDIYYQGKELEAKRKENRPGLLSDELRV-ALGMTTGPNSDKSPPPWLIAQQRYGPPPS 458
           KFG +Y + + L+ ++K         ++R     + TGP     PPP         PPP 
Sbjct: 94  KFGPMYKRDRALKQQKKA--------QIRANGFKLETGPPMGVPPPP--------PPPPD 137

Query: 459 YPNLKIPGLNKGIPPGCSFGFHAGGWGKPPVDDDGKP 569
           Y  +  PGL+   P G + G  AG     P+ D G P
Sbjct: 138 Y--MLPPGLHVPEPKGLASGPPAG-----PLGDFGAP 167
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88,521,062
Number of Sequences: 369166
Number of extensions: 1795709
Number of successful extensions: 5661
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5203
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5569
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9078450570
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)