Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_D17-2
(340 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P24485|CD53_RAT Leukocyte surface antigen CD53 (Cell sur... 53 2e-07
sp|P19397|CD53_HUMAN Leukocyte surface antigen CD53 (Cell s... 52 6e-07
sp|Q61451|CD53_MOUSE Leukocyte surface antigen CD53 (Cell s... 51 7e-07
sp|P19075|TSN8_HUMAN Tetraspanin-8 (Tspan-8) (Transmembrane... 47 1e-05
sp|Q80WR1|TSN18_MOUSE Tetraspanin-18 (Tspan-18) 45 7e-05
sp|O35566|C151_MOUSE Platelet-endothelial tetraspan antigen... 45 7e-05
sp|Q9QZA6|CD151_RAT Platelet endothelial tetraspan antigen ... 45 7e-05
sp|P61171|CD151_MACMU Platelet-endothelial tetraspan antige... 43 3e-04
sp|O60635|TSN1_HUMAN Tetraspanin-1 (Tspan-1) (Tetraspan NET... 42 3e-04
sp|Q58CY8|TSN18_BOVIN Tetraspanin-18 (Tspan-18) 42 3e-04
>sp|P24485|CD53_RAT Leukocyte surface antigen CD53 (Cell surface glycoprotein CD53)
(Leukocyte antigen MRC OX-44)
Length = 219
Score = 53.1 bits (126), Expect = 2e-07
Identities = 36/108 (33%), Positives = 56/108 (51%), Gaps = 3/108 (2%)
Frame = +2
Query: 17 MCSLKSIN--IFSYSFLVLLSILCG-GFGGWLISQEHWTILLFGTQTIQSMGWVLVAAGI 187
M SLK + +F ++FL + C GFG L+ Q + IL F ++G VLV G
Sbjct: 3 MSSLKLLKYVLFFFNFLFWVCGCCILGFGIHLLVQNTYGIL-FRNLPFLTLGNVLVIVGS 61
Query: 188 LIFLTAIFGCIGTIKESRCFIIMFXXXXXXXXXXXXXSGTLLFIYKKQ 331
+I + A GC+G+IKE++C ++ F LLF+Y+K+
Sbjct: 62 IIMVVAFLGCMGSIKENKCLLMSFFVLLLLILLAEVTLAILLFVYEKK 109
>sp|P19397|CD53_HUMAN Leukocyte surface antigen CD53 (Cell surface glycoprotein CD53)
(Tetraspanin-25) (Tspan-25)
Length = 219
Score = 51.6 bits (122), Expect = 6e-07
Identities = 32/109 (29%), Positives = 56/109 (51%), Gaps = 4/109 (3%)
Frame = +2
Query: 17 MCSLKSINIFSYSFLVLLSILCG----GFGGWLISQEHWTILLFGTQTIQSMGWVLVAAG 184
M SLK + + F LL +CG GFG +L+ ++ +L ++ ++G V V G
Sbjct: 3 MSSLKLLKYVLF-FFNLLFWICGCCILGFGIYLLIHNNFGVLFHNLPSL-TLGNVFVIVG 60
Query: 185 ILIFLTAIFGCIGTIKESRCFIIMFXXXXXXXXXXXXXSGTLLFIYKKQ 331
+I + A GC+G+IKE++C ++ F LLF+Y+++
Sbjct: 61 SIIMVVAFLGCMGSIKENKCLLMSFFILLLIILLAEVTLAILLFVYEQK 109
>sp|Q61451|CD53_MOUSE Leukocyte surface antigen CD53 (Cell surface glycoprotein CD53)
Length = 219
Score = 51.2 bits (121), Expect = 7e-07
Identities = 32/109 (29%), Positives = 55/109 (50%), Gaps = 4/109 (3%)
Frame = +2
Query: 17 MCSLKSINIFSYSFLVLLSILCG----GFGGWLISQEHWTILLFGTQTIQSMGWVLVAAG 184
M SLK + + F +L + CG GFG + + Q + +L F ++G +LV G
Sbjct: 3 MSSLKLLKYVLFIFNLLFWV-CGCCILGFGIYFLVQNTYGVL-FRNLPFLTLGNILVIVG 60
Query: 185 ILIFLTAIFGCIGTIKESRCFIIMFXXXXXXXXXXXXXSGTLLFIYKKQ 331
+I + A GC+G+IKE++C ++ F LLF+Y+++
Sbjct: 61 SIIMVVAFLGCMGSIKENKCLLMSFFVLLLIILLAEVTIAILLFVYEQK 109
>sp|P19075|TSN8_HUMAN Tetraspanin-8 (Tspan-8) (Transmembrane 4 superfamily member 3)
(Tumor-associated antigen CO-029)
Length = 237
Score = 47.4 bits (111), Expect = 1e-05
Identities = 30/106 (28%), Positives = 54/106 (50%), Gaps = 6/106 (5%)
Frame = +2
Query: 38 NIFSYSFLV-LLSILCGGFGGWL-ISQEHWTILLFGTQTIQSMGWV----LVAAGILIFL 199
++F+++FL L IL W+ +S + I FG++ + S +V L+A G +I +
Sbjct: 11 SMFTFNFLFWLCGILILALAIWVRVSNDSQAI--FGSEDVGSSSYVAVDILIAVGAIIMI 68
Query: 200 TAIFGCIGTIKESRCFIIMFXXXXXXXXXXXXXSGTLLFIYKKQAE 337
GC G IKESRC +++F +G L ++K +++
Sbjct: 69 LGFLGCCGAIKESRCMLLLFFIGLLLILLLQVATGILGAVFKSKSD 114
>sp|Q80WR1|TSN18_MOUSE Tetraspanin-18 (Tspan-18)
Length = 248
Score = 44.7 bits (104), Expect = 7e-05
Identities = 25/98 (25%), Positives = 47/98 (47%), Gaps = 2/98 (2%)
Frame = +2
Query: 41 IFSYSFLVLLSILCG-GFGGWLISQEHWTILLFGTQTIQSMG-WVLVAAGILIFLTAIFG 214
+F ++F V L C G G W++ + T + + G ++++A G L+FL G
Sbjct: 13 MFVFNFFVFLGGACLLGVGIWVLVDPTGFREIVATNPLLTTGAYIVLAMGGLLFLLGFLG 72
Query: 215 CIGTIKESRCFIIMFXXXXXXXXXXXXXSGTLLFIYKK 328
C G ++E+RC ++ F + L FI+++
Sbjct: 73 CCGAVRENRCLLLFFFLFILIIFLVELSAAILAFIFRE 110
>sp|O35566|C151_MOUSE Platelet-endothelial tetraspan antigen 3 (PETA-3) (GP27) (Membrane
glycoprotein SFA-1) (CD151 antigen)
Length = 253
Score = 44.7 bits (104), Expect = 7e-05
Identities = 24/100 (24%), Positives = 50/100 (50%), Gaps = 3/100 (3%)
Frame = +2
Query: 41 IFSYS---FLVLLSILCGGFGGWLISQEHWTILLFGTQTIQSMGWVLVAAGILIFLTAIF 211
+F+Y+ +L L+++ G W ++ + I L + T + ++LV AG+++ +T +
Sbjct: 20 LFTYNCCFWLAGLAVMAVGI--WTLALKSDYISLLASSTYLATAYILVVAGVVVMVTGVL 77
Query: 212 GCIGTIKESRCFIIMFXXXXXXXXXXXXXSGTLLFIYKKQ 331
GC T KE R + ++ +G L ++Y +Q
Sbjct: 78 GCCATFKERRNLLRLYFILLLIIFLLEIIAGILAYVYYQQ 117
>sp|Q9QZA6|CD151_RAT Platelet endothelial tetraspan antigen 3 (CD151 antigen)
Length = 253
Score = 44.7 bits (104), Expect = 7e-05
Identities = 24/100 (24%), Positives = 50/100 (50%), Gaps = 3/100 (3%)
Frame = +2
Query: 41 IFSYS---FLVLLSILCGGFGGWLISQEHWTILLFGTQTIQSMGWVLVAAGILIFLTAIF 211
+F+Y+ +L L+++ G W ++ + I L + T + ++LV AG+++ +T +
Sbjct: 20 LFTYNCCFWLAGLAVMAVGI--WTLALKSDYISLLASSTYLATAYILVVAGVVVMVTGVL 77
Query: 212 GCIGTIKESRCFIIMFXXXXXXXXXXXXXSGTLLFIYKKQ 331
GC T KE R + ++ +G L ++Y +Q
Sbjct: 78 GCCATFKERRNLLRLYFILLLIIFLLEIIAGILAYVYYQQ 117
>sp|P61171|CD151_MACMU Platelet-endothelial tetraspan antigen 3 (PETA-3) (CD151 antigen)
sp|P61170|CD151_CERAE Platelet-endothelial tetraspan antigen 3 (PETA-3) (CD151 antigen)
Length = 253
Score = 42.7 bits (99), Expect = 3e-04
Identities = 24/100 (24%), Positives = 49/100 (49%), Gaps = 3/100 (3%)
Frame = +2
Query: 41 IFSYS---FLVLLSILCGGFGGWLISQEHWTILLFGTQTIQSMGWVLVAAGILIFLTAIF 211
+F+Y+ +L L+++ G W ++ + I L + T + ++LV AG ++ +T +
Sbjct: 20 LFTYNCCFWLAGLAVMAVGI--WTLALKSDYISLLASGTYLATAYILVVAGAVVMVTGVL 77
Query: 212 GCIGTIKESRCFIIMFXXXXXXXXXXXXXSGTLLFIYKKQ 331
GC T KE R + ++ +G L ++Y +Q
Sbjct: 78 GCCATFKERRNLLRLYFILLLIIFLLEIIAGVLAYVYYQQ 117
>sp|O60635|TSN1_HUMAN Tetraspanin-1 (Tspan-1) (Tetraspan NET-1) (Tetraspanin TM4-C)
Length = 241
Score = 42.4 bits (98), Expect = 3e-04
Identities = 26/100 (26%), Positives = 42/100 (42%), Gaps = 9/100 (9%)
Frame = +2
Query: 65 LLSILCGG----FGGWLISQEHWTILLFGTQTIQSM-----GWVLVAAGILIFLTAIFGC 217
LL LCG G W+ + +FG + +M G+ L+AAG+++F GC
Sbjct: 16 LLIFLCGAALLAVGIWVSIDGASFLKIFGPLSSSAMQFVNVGYFLIAAGVVVFALGFLGC 75
Query: 218 IGTIKESRCFIIMFXXXXXXXXXXXXXSGTLLFIYKKQAE 337
G ES+C ++ F + + +Y AE
Sbjct: 76 YGAKTESKCALVTFFFILLLIFIAEVAAAVVALVYTTMAE 115
>sp|Q58CY8|TSN18_BOVIN Tetraspanin-18 (Tspan-18)
Length = 249
Score = 42.4 bits (98), Expect = 3e-04
Identities = 22/97 (22%), Positives = 46/97 (47%), Gaps = 2/97 (2%)
Frame = +2
Query: 41 IFSYSFLVLLSILCG-GFGGW-LISQEHWTILLFGTQTIQSMGWVLVAAGILIFLTAIFG 214
+F ++F + L C G G W ++ + ++ + + ++L+A G L+FL G
Sbjct: 13 MFVFNFFIFLGGACLLGIGIWVMVDPTGFREIVAANPLLITGAYILLAMGGLLFLLGFLG 72
Query: 215 CIGTIKESRCFIIMFXXXXXXXXXXXXXSGTLLFIYK 325
C G ++E++C ++ F + L FI++
Sbjct: 73 CCGAVRENKCLLLFFFLFILIIFLAELSAAILAFIFR 109
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,635,544
Number of Sequences: 369166
Number of extensions: 635838
Number of successful extensions: 1829
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1804
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1828
length of database: 68,354,980
effective HSP length: 80
effective length of database: 53,576,180
effective search space used: 1714437760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)