Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Dr_sW_011_D01-1 (443 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|Q9VRV7|PM14_DROME Pre-mRNA branch site p14-like protein 168 6e-42 sp|Q9Y3B4|PM14_HUMAN Pre-mRNA branch site protein p14 (SF3B... 155 3e-38 sp|Q8ITY4|PM14_CAEEL Pre-mRNA branch site p14-like protein 149 4e-36 sp|Q9FMP4|PM14_ARATH Pre-mRNA branch site p14-like protein 138 5e-33 sp|O97018|SXL_CHRRU Sex-lethal protein homolog 50 2e-06 sp|O17310|SXL_MUSDO Sex-lethal protein homolog 49 4e-06 sp|Q24562|U2AF2_DROME Splicing factor U2AF 50 kDa subunit (... 49 5e-06 sp|O22315|SFRS1_ARATH Pre-mRNA splicing factor SF2 (SR1 pro... 48 8e-06 sp|O61374|SXL_CERCA Sex-lethal protein homolog (CCSXL) 48 8e-06 sp|P19339|SXL_DROME Sex-lethal protein 47 1e-05
>sp|Q9VRV7|PM14_DROME Pre-mRNA branch site p14-like protein Length = 121 Score = 168 bits (425), Expect = 6e-42 Identities = 74/111 (66%), Positives = 92/111 (82%) Frame = +1 Query: 31 KLPPNVSRILMVSNLPYELRDDEMYDIFGKYGTIRQIRVGNTTENKGKAFVVYEDIYDAQ 210 +LPP V+R+L V NLPY++ DEMYDIFGK+G IRQIRVGNT E +G AFVVYEDI+DA+ Sbjct: 8 RLPPEVNRLLYVRNLPYKITSDEMYDIFGKFGAIRQIRVGNTPETRGTAFVVYEDIFDAK 67 Query: 211 NACEHLTGFNVCNRYICISYYQPNKVFKKVDYTAKNEELNNLKSKFGIGTP 363 NAC+HL+GFNVCNRY+ + YYQ NK FK+VD K EELNN+K+K+ + TP Sbjct: 68 NACDHLSGFNVCNRYLVVLYYQSNKAFKRVDMDKKQEELNNIKAKYNLKTP 118
>sp|Q9Y3B4|PM14_HUMAN Pre-mRNA branch site protein p14 (SF3B 14 kDa subunit) sp|P59708|PM14_MOUSE Pre-mRNA branch site protein p14 (SF3B 14 kDa subunit) Length = 125 Score = 155 bits (393), Expect = 3e-38 Identities = 69/110 (62%), Positives = 88/110 (80%) Frame = +1 Query: 31 KLPPNVSRILMVSNLPYELRDDEMYDIFGKYGTIRQIRVGNTTENKGKAFVVYEDIYDAQ 210 +LPP V+RIL + NLPY++ +EMYDIFGKYG IRQIRVGNT E +G A+VVYEDI+DA+ Sbjct: 12 RLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDAK 71 Query: 211 NACEHLTGFNVCNRYICISYYQPNKVFKKVDYTAKNEELNNLKSKFGIGT 360 NAC+HL+GFNVCNRY+ + YY N+ F+K+D K E+L LK K+GI T Sbjct: 72 NACDHLSGFNVCNRYLVVLYYNANRAFQKMDTKKKEEQLKLLKEKYGINT 121
>sp|Q8ITY4|PM14_CAEEL Pre-mRNA branch site p14-like protein Length = 138 Score = 149 bits (375), Expect = 4e-36 Identities = 68/131 (51%), Positives = 95/131 (72%) Frame = +1 Query: 4 MAFSNKRISKLPPNVSRILMVSNLPYELRDDEMYDIFGKYGTIRQIRVGNTTENKGKAFV 183 MA R +KLPP V+RIL + NLPY++ +EMY+IFGK+G +RQIRVGNT E +G AFV Sbjct: 3 MANRQNRGAKLPPEVNRILYIKNLPYKITTEEMYEIFGKFGAVRQIRVGNTAETRGTAFV 62 Query: 184 VYEDIYDAQNACEHLTGFNVCNRYICISYYQPNKVFKKVDYTAKNEELNNLKSKFGIGTP 363 VYEDI+DA+ ACEHL+G+NV NRY+ + YYQ K +K++D +L ++K ++GIG Sbjct: 63 VYEDIFDAKTACEHLSGYNVSNRYLVVLYYQATKAWKRMDTEKARTKLEDIKERYGIGGD 122 Query: 364 RNNVK*KVRVF 396 + K K+ +F Sbjct: 123 IDKEKEKLGLF 133
>sp|Q9FMP4|PM14_ARATH Pre-mRNA branch site p14-like protein Length = 124 Score = 138 bits (348), Expect = 5e-33 Identities = 63/114 (55%), Positives = 82/114 (71%) Frame = +1 Query: 19 KRISKLPPNVSRILMVSNLPYELRDDEMYDIFGKYGTIRQIRVGNTTENKGKAFVVYEDI 198 K ++LPP V+R+L V NLP+ + +EMYDIFGKYG IRQIR+G KG AFVVYEDI Sbjct: 8 KSNTRLPPEVNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGCDKATKGTAFVVYEDI 67 Query: 199 YDAQNACEHLTGFNVCNRYICISYYQPNKVFKKVDYTAKNEELNNLKSKFGIGT 360 YDA+NA +HL+GFNV NRY+ + YYQ K+ KK D +E+ L+ K+G+ T Sbjct: 68 YDAKNAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKSEDEITKLQEKYGVST 121
>sp|O97018|SXL_CHRRU Sex-lethal protein homolog Length = 307 Score = 50.4 bits (119), Expect = 2e-06 Identities = 36/121 (29%), Positives = 59/121 (48%), Gaps = 11/121 (9%) Frame = +1 Query: 58 LMVSNLPYELRDDEMYDIFGKYGTIRQIRVGN---TTENKGKAFVVYEDIYDAQNACEHL 228 L+V+ LP ++ D E+Y +F G I R+ T + G AFV + DAQNA + L Sbjct: 87 LIVNYLPQDMTDRELYALFRTCGPINTCRIMKDYKTGYSFGYAFVDFASEIDAQNAIKSL 146 Query: 229 TGFNVCNRYICISYYQP--------NKVFKKVDYTAKNEELNNLKSKFGIGTPRNNVK*K 384 G V N+ + +SY +P N + T ++EL + K+G +N ++ K Sbjct: 147 NGVTVRNKRLKVSYARPGGESIKDTNLYVTNLPRTITDDELEKIFGKYGNIVQKNILRDK 206 Query: 385 V 387 + Sbjct: 207 L 207
Score = 41.6 bits (96), Expect = 8e-04 Identities = 26/60 (43%), Positives = 34/60 (56%), Gaps = 3/60 (5%) Frame = +1 Query: 58 LMVSNLPYELRDDEMYDIFGKYGTIRQ---IRVGNTTENKGKAFVVYEDIYDAQNACEHL 228 L V+NLP + DDE+ IFGKYG I Q +R T + +G AFV + +AQ A L Sbjct: 173 LYVTNLPRTITDDELEKIFGKYGNIVQKNILRDKLTGKPRGVAFVRFNKREEAQEAISAL 232
>sp|O17310|SXL_MUSDO Sex-lethal protein homolog Length = 324 Score = 49.3 bits (116), Expect = 4e-06 Identities = 35/121 (28%), Positives = 59/121 (48%), Gaps = 11/121 (9%) Frame = +1 Query: 58 LMVSNLPYELRDDEMYDIFGKYGTIRQIRVGN---TTENKGKAFVVYEDIYDAQNACEHL 228 L+V+ LP ++ D E+Y +F G I R+ T + G AFV + DAQNA + + Sbjct: 104 LIVNYLPQDMTDRELYALFRTCGPINTCRIMKDYKTGYSFGYAFVDFASEIDAQNAIKTV 163 Query: 229 TGFNVCNRYICISYYQP--------NKVFKKVDYTAKNEELNNLKSKFGIGTPRNNVK*K 384 G V N+ + +SY +P N + T ++EL + K+G +N ++ K Sbjct: 164 NGITVRNKRLKVSYARPGGESIKDTNLYVTNLPRTITDDELEKIFGKYGNIVQKNILRDK 223 Query: 385 V 387 + Sbjct: 224 L 224
Score = 41.2 bits (95), Expect = 0.001 Identities = 26/60 (43%), Positives = 33/60 (55%), Gaps = 3/60 (5%) Frame = +1 Query: 58 LMVSNLPYELRDDEMYDIFGKYGTIRQ---IRVGNTTENKGKAFVVYEDIYDAQNACEHL 228 L V+NLP + DDE+ IFGKYG I Q +R T +G AFV + +AQ A L Sbjct: 190 LYVTNLPRTITDDELEKIFGKYGNIVQKNILRDKLTGRPRGVAFVRFNKREEAQEAISAL 249
>sp|Q24562|U2AF2_DROME Splicing factor U2AF 50 kDa subunit (U2 auxiliary factor 50 kDa subunit) (U2 snRNP auxiliary factor large subunit) Length = 416 Score = 48.9 bits (115), Expect = 5e-06 Identities = 30/100 (30%), Positives = 45/100 (45%), Gaps = 13/100 (13%) Frame = +1 Query: 37 PPNVSRILMVSNLPYELRDDEMYDIF--------GKYGTIRQIRVGNTTENK-----GKA 177 PP L+ P ELRD+E Y+ KYG +R + + E GK Sbjct: 315 PPTEVLCLLNMVTPDELRDEEEYEDILEDIKEECTKYGVVRSVEIPRPIEGVEVPGCGKV 374 Query: 178 FVVYEDIYDAQNACEHLTGFNVCNRYICISYYQPNKVFKK 297 FV + + D Q A + LTG +R + SY+ P+K ++ Sbjct: 375 FVEFNSVLDCQKAQQALTGRKFSDRVVVTSYFDPDKYHRR 414
Score = 33.1 bits (74), Expect = 0.28 Identities = 20/83 (24%), Positives = 38/83 (45%), Gaps = 3/83 (3%) Frame = +1 Query: 25 ISKLPPNVSRILMVSNLPYELRDDEMYDI---FGKYGTIRQIRVGNTTENKGKAFVVYED 195 IS + P+ + + LP L DD++ ++ FGK ++ T +KG AF Y D Sbjct: 198 ISTVVPDSPHKIFIGGLPNYLNDDQVKELLLSFGKLRAFNLVKDAATGLSKGYAFCEYVD 257 Query: 196 IYDAQNACEHLTGFNVCNRYICI 264 + + L G + ++ + + Sbjct: 258 LSITDQSIAGLNGMQLGDKKLIV 280
>sp|O22315|SFRS1_ARATH Pre-mRNA splicing factor SF2 (SR1 protein) Length = 303 Score = 48.1 bits (113), Expect = 8e-06 Identities = 24/64 (37%), Positives = 38/64 (59%) Frame = +1 Query: 49 SRILMVSNLPYELRDDEMYDIFGKYGTIRQIRVGNTTENKGKAFVVYEDIYDAQNACEHL 228 SR + V NLP ++R+ E+ D+F KYG + QI + G AFV ++D DA++A Sbjct: 6 SRTVYVGNLPGDIREREVEDLFSKYGPVVQIDLKVPPRPPGYAFVEFDDARDAEDAIHGR 65 Query: 229 TGFN 240 G++ Sbjct: 66 DGYD 69
>sp|O61374|SXL_CERCA Sex-lethal protein homolog (CCSXL) Length = 348 Score = 48.1 bits (113), Expect = 8e-06 Identities = 34/121 (28%), Positives = 61/121 (50%), Gaps = 11/121 (9%) Frame = +1 Query: 58 LMVSNLPYELRDDEMYDIF---GKYGTIRQIRVGNTTENKGKAFVVYEDIYDAQNACEHL 228 L+V+ LP ++ D E+Y +F G T R +R T + G AFV + D+Q A + L Sbjct: 112 LIVNYLPQDMTDRELYALFRTIGPINTCRIMRDYKTGYSFGYAFVDFAAETDSQRAIKSL 171 Query: 229 TGFNVCNRYICISYYQP--------NKVFKKVDYTAKNEELNNLKSKFGIGTPRNNVK*K 384 G V N+ + +SY +P N + T +++L+ + K+G+ +N ++ K Sbjct: 172 NGITVRNKRLKVSYARPGGESIKDTNLYVTNLPRTITDDQLDTIFGKYGMIVQKNILRDK 231 Query: 385 V 387 + Sbjct: 232 L 232
Score = 39.7 bits (91), Expect = 0.003 Identities = 25/60 (41%), Positives = 34/60 (56%), Gaps = 3/60 (5%) Frame = +1 Query: 58 LMVSNLPYELRDDEMYDIFGKYGTIRQ---IRVGNTTENKGKAFVVYEDIYDAQNACEHL 228 L V+NLP + DD++ IFGKYG I Q +R T + +G AFV + +AQ A L Sbjct: 198 LYVTNLPRTITDDQLDTIFGKYGMIVQKNILRDKLTGKPRGVAFVRFNKREEAQEAISAL 257
>sp|P19339|SXL_DROME Sex-lethal protein Length = 354 Score = 47.4 bits (111), Expect = 1e-05 Identities = 34/121 (28%), Positives = 60/121 (49%), Gaps = 11/121 (9%) Frame = +1 Query: 58 LMVSNLPYELRDDEMYDIF---GKYGTIRQIRVGNTTENKGKAFVVYEDIYDAQNACEHL 228 L+V+ LP ++ D E+Y +F G T R +R T + G AFV + D+Q A + L Sbjct: 127 LIVNYLPQDMTDRELYALFRAIGPINTCRIMRDYKTGYSFGYAFVDFTSEMDSQRAIKVL 186 Query: 229 TGFNVCNRYICISYYQP--------NKVFKKVDYTAKNEELNNLKSKFGIGTPRNNVK*K 384 G V N+ + +SY +P N + T +++L+ + K+G +N ++ K Sbjct: 187 NGITVRNKRLKVSYARPGGESIKDTNLYVTNLPRTITDDQLDTIFGKYGSIVQKNILRDK 246 Query: 385 V 387 + Sbjct: 247 L 247
Score = 41.6 bits (96), Expect = 8e-04 Identities = 26/60 (43%), Positives = 34/60 (56%), Gaps = 3/60 (5%) Frame = +1 Query: 58 LMVSNLPYELRDDEMYDIFGKYGTIRQ---IRVGNTTENKGKAFVVYEDIYDAQNACEHL 228 L V+NLP + DD++ IFGKYG+I Q +R T +G AFV Y +AQ A L Sbjct: 213 LYVTNLPRTITDDQLDTIFGKYGSIVQKNILRDKLTGRPRGVAFVRYNKREEAQEAISAL 272
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 50,294,724 Number of Sequences: 369166 Number of extensions: 978234 Number of successful extensions: 3223 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 3070 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 3200 length of database: 68,354,980 effective HSP length: 100 effective length of database: 49,881,480 effective search space used: 2344429560 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)