Planarian EST Database


Dr_sW_011_D01-1

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_011_D01-1
         (443 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9VRV7|PM14_DROME  Pre-mRNA branch site p14-like protein       168   6e-42
sp|Q9Y3B4|PM14_HUMAN  Pre-mRNA branch site protein p14 (SF3B...   155   3e-38
sp|Q8ITY4|PM14_CAEEL  Pre-mRNA branch site p14-like protein       149   4e-36
sp|Q9FMP4|PM14_ARATH  Pre-mRNA branch site p14-like protein       138   5e-33
sp|O97018|SXL_CHRRU  Sex-lethal protein homolog                    50   2e-06
sp|O17310|SXL_MUSDO  Sex-lethal protein homolog                    49   4e-06
sp|Q24562|U2AF2_DROME  Splicing factor U2AF 50 kDa subunit (...    49   5e-06
sp|O22315|SFRS1_ARATH  Pre-mRNA splicing factor SF2 (SR1 pro...    48   8e-06
sp|O61374|SXL_CERCA  Sex-lethal protein homolog (CCSXL)            48   8e-06
sp|P19339|SXL_DROME  Sex-lethal protein                            47   1e-05
>sp|Q9VRV7|PM14_DROME Pre-mRNA branch site p14-like protein
          Length = 121

 Score =  168 bits (425), Expect = 6e-42
 Identities = 74/111 (66%), Positives = 92/111 (82%)
 Frame = +1

Query: 31  KLPPNVSRILMVSNLPYELRDDEMYDIFGKYGTIRQIRVGNTTENKGKAFVVYEDIYDAQ 210
           +LPP V+R+L V NLPY++  DEMYDIFGK+G IRQIRVGNT E +G AFVVYEDI+DA+
Sbjct: 8   RLPPEVNRLLYVRNLPYKITSDEMYDIFGKFGAIRQIRVGNTPETRGTAFVVYEDIFDAK 67

Query: 211 NACEHLTGFNVCNRYICISYYQPNKVFKKVDYTAKNEELNNLKSKFGIGTP 363
           NAC+HL+GFNVCNRY+ + YYQ NK FK+VD   K EELNN+K+K+ + TP
Sbjct: 68  NACDHLSGFNVCNRYLVVLYYQSNKAFKRVDMDKKQEELNNIKAKYNLKTP 118
>sp|Q9Y3B4|PM14_HUMAN Pre-mRNA branch site protein p14 (SF3B 14 kDa subunit)
 sp|P59708|PM14_MOUSE Pre-mRNA branch site protein p14 (SF3B 14 kDa subunit)
          Length = 125

 Score =  155 bits (393), Expect = 3e-38
 Identities = 69/110 (62%), Positives = 88/110 (80%)
 Frame = +1

Query: 31  KLPPNVSRILMVSNLPYELRDDEMYDIFGKYGTIRQIRVGNTTENKGKAFVVYEDIYDAQ 210
           +LPP V+RIL + NLPY++  +EMYDIFGKYG IRQIRVGNT E +G A+VVYEDI+DA+
Sbjct: 12  RLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDAK 71

Query: 211 NACEHLTGFNVCNRYICISYYQPNKVFKKVDYTAKNEELNNLKSKFGIGT 360
           NAC+HL+GFNVCNRY+ + YY  N+ F+K+D   K E+L  LK K+GI T
Sbjct: 72  NACDHLSGFNVCNRYLVVLYYNANRAFQKMDTKKKEEQLKLLKEKYGINT 121
>sp|Q8ITY4|PM14_CAEEL Pre-mRNA branch site p14-like protein
          Length = 138

 Score =  149 bits (375), Expect = 4e-36
 Identities = 68/131 (51%), Positives = 95/131 (72%)
 Frame = +1

Query: 4   MAFSNKRISKLPPNVSRILMVSNLPYELRDDEMYDIFGKYGTIRQIRVGNTTENKGKAFV 183
           MA    R +KLPP V+RIL + NLPY++  +EMY+IFGK+G +RQIRVGNT E +G AFV
Sbjct: 3   MANRQNRGAKLPPEVNRILYIKNLPYKITTEEMYEIFGKFGAVRQIRVGNTAETRGTAFV 62

Query: 184 VYEDIYDAQNACEHLTGFNVCNRYICISYYQPNKVFKKVDYTAKNEELNNLKSKFGIGTP 363
           VYEDI+DA+ ACEHL+G+NV NRY+ + YYQ  K +K++D      +L ++K ++GIG  
Sbjct: 63  VYEDIFDAKTACEHLSGYNVSNRYLVVLYYQATKAWKRMDTEKARTKLEDIKERYGIGGD 122

Query: 364 RNNVK*KVRVF 396
            +  K K+ +F
Sbjct: 123 IDKEKEKLGLF 133
>sp|Q9FMP4|PM14_ARATH Pre-mRNA branch site p14-like protein
          Length = 124

 Score =  138 bits (348), Expect = 5e-33
 Identities = 63/114 (55%), Positives = 82/114 (71%)
 Frame = +1

Query: 19  KRISKLPPNVSRILMVSNLPYELRDDEMYDIFGKYGTIRQIRVGNTTENKGKAFVVYEDI 198
           K  ++LPP V+R+L V NLP+ +  +EMYDIFGKYG IRQIR+G     KG AFVVYEDI
Sbjct: 8   KSNTRLPPEVNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGCDKATKGTAFVVYEDI 67

Query: 199 YDAQNACEHLTGFNVCNRYICISYYQPNKVFKKVDYTAKNEELNNLKSKFGIGT 360
           YDA+NA +HL+GFNV NRY+ + YYQ  K+ KK D     +E+  L+ K+G+ T
Sbjct: 68  YDAKNAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKSEDEITKLQEKYGVST 121
>sp|O97018|SXL_CHRRU Sex-lethal protein homolog
          Length = 307

 Score = 50.4 bits (119), Expect = 2e-06
 Identities = 36/121 (29%), Positives = 59/121 (48%), Gaps = 11/121 (9%)
 Frame = +1

Query: 58  LMVSNLPYELRDDEMYDIFGKYGTIRQIRVGN---TTENKGKAFVVYEDIYDAQNACEHL 228
           L+V+ LP ++ D E+Y +F   G I   R+     T  + G AFV +    DAQNA + L
Sbjct: 87  LIVNYLPQDMTDRELYALFRTCGPINTCRIMKDYKTGYSFGYAFVDFASEIDAQNAIKSL 146

Query: 229 TGFNVCNRYICISYYQP--------NKVFKKVDYTAKNEELNNLKSKFGIGTPRNNVK*K 384
            G  V N+ + +SY +P        N     +  T  ++EL  +  K+G    +N ++ K
Sbjct: 147 NGVTVRNKRLKVSYARPGGESIKDTNLYVTNLPRTITDDELEKIFGKYGNIVQKNILRDK 206

Query: 385 V 387
           +
Sbjct: 207 L 207

 Score = 41.6 bits (96), Expect = 8e-04
 Identities = 26/60 (43%), Positives = 34/60 (56%), Gaps = 3/60 (5%)
 Frame = +1

Query: 58  LMVSNLPYELRDDEMYDIFGKYGTIRQ---IRVGNTTENKGKAFVVYEDIYDAQNACEHL 228
           L V+NLP  + DDE+  IFGKYG I Q   +R   T + +G AFV +    +AQ A   L
Sbjct: 173 LYVTNLPRTITDDELEKIFGKYGNIVQKNILRDKLTGKPRGVAFVRFNKREEAQEAISAL 232
>sp|O17310|SXL_MUSDO Sex-lethal protein homolog
          Length = 324

 Score = 49.3 bits (116), Expect = 4e-06
 Identities = 35/121 (28%), Positives = 59/121 (48%), Gaps = 11/121 (9%)
 Frame = +1

Query: 58  LMVSNLPYELRDDEMYDIFGKYGTIRQIRVGN---TTENKGKAFVVYEDIYDAQNACEHL 228
           L+V+ LP ++ D E+Y +F   G I   R+     T  + G AFV +    DAQNA + +
Sbjct: 104 LIVNYLPQDMTDRELYALFRTCGPINTCRIMKDYKTGYSFGYAFVDFASEIDAQNAIKTV 163

Query: 229 TGFNVCNRYICISYYQP--------NKVFKKVDYTAKNEELNNLKSKFGIGTPRNNVK*K 384
            G  V N+ + +SY +P        N     +  T  ++EL  +  K+G    +N ++ K
Sbjct: 164 NGITVRNKRLKVSYARPGGESIKDTNLYVTNLPRTITDDELEKIFGKYGNIVQKNILRDK 223

Query: 385 V 387
           +
Sbjct: 224 L 224

 Score = 41.2 bits (95), Expect = 0.001
 Identities = 26/60 (43%), Positives = 33/60 (55%), Gaps = 3/60 (5%)
 Frame = +1

Query: 58  LMVSNLPYELRDDEMYDIFGKYGTIRQ---IRVGNTTENKGKAFVVYEDIYDAQNACEHL 228
           L V+NLP  + DDE+  IFGKYG I Q   +R   T   +G AFV +    +AQ A   L
Sbjct: 190 LYVTNLPRTITDDELEKIFGKYGNIVQKNILRDKLTGRPRGVAFVRFNKREEAQEAISAL 249
>sp|Q24562|U2AF2_DROME Splicing factor U2AF 50 kDa subunit (U2 auxiliary factor 50 kDa
           subunit) (U2 snRNP auxiliary factor large subunit)
          Length = 416

 Score = 48.9 bits (115), Expect = 5e-06
 Identities = 30/100 (30%), Positives = 45/100 (45%), Gaps = 13/100 (13%)
 Frame = +1

Query: 37  PPNVSRILMVSNLPYELRDDEMYDIF--------GKYGTIRQIRVGNTTENK-----GKA 177
           PP     L+    P ELRD+E Y+           KYG +R + +    E       GK 
Sbjct: 315 PPTEVLCLLNMVTPDELRDEEEYEDILEDIKEECTKYGVVRSVEIPRPIEGVEVPGCGKV 374

Query: 178 FVVYEDIYDAQNACEHLTGFNVCNRYICISYYQPNKVFKK 297
           FV +  + D Q A + LTG    +R +  SY+ P+K  ++
Sbjct: 375 FVEFNSVLDCQKAQQALTGRKFSDRVVVTSYFDPDKYHRR 414

 Score = 33.1 bits (74), Expect = 0.28
 Identities = 20/83 (24%), Positives = 38/83 (45%), Gaps = 3/83 (3%)
 Frame = +1

Query: 25  ISKLPPNVSRILMVSNLPYELRDDEMYDI---FGKYGTIRQIRVGNTTENKGKAFVVYED 195
           IS + P+    + +  LP  L DD++ ++   FGK      ++   T  +KG AF  Y D
Sbjct: 198 ISTVVPDSPHKIFIGGLPNYLNDDQVKELLLSFGKLRAFNLVKDAATGLSKGYAFCEYVD 257

Query: 196 IYDAQNACEHLTGFNVCNRYICI 264
           +     +   L G  + ++ + +
Sbjct: 258 LSITDQSIAGLNGMQLGDKKLIV 280
>sp|O22315|SFRS1_ARATH Pre-mRNA splicing factor SF2 (SR1 protein)
          Length = 303

 Score = 48.1 bits (113), Expect = 8e-06
 Identities = 24/64 (37%), Positives = 38/64 (59%)
 Frame = +1

Query: 49  SRILMVSNLPYELRDDEMYDIFGKYGTIRQIRVGNTTENKGKAFVVYEDIYDAQNACEHL 228
           SR + V NLP ++R+ E+ D+F KYG + QI +       G AFV ++D  DA++A    
Sbjct: 6   SRTVYVGNLPGDIREREVEDLFSKYGPVVQIDLKVPPRPPGYAFVEFDDARDAEDAIHGR 65

Query: 229 TGFN 240
            G++
Sbjct: 66  DGYD 69
>sp|O61374|SXL_CERCA Sex-lethal protein homolog (CCSXL)
          Length = 348

 Score = 48.1 bits (113), Expect = 8e-06
 Identities = 34/121 (28%), Positives = 61/121 (50%), Gaps = 11/121 (9%)
 Frame = +1

Query: 58  LMVSNLPYELRDDEMYDIF---GKYGTIRQIRVGNTTENKGKAFVVYEDIYDAQNACEHL 228
           L+V+ LP ++ D E+Y +F   G   T R +R   T  + G AFV +    D+Q A + L
Sbjct: 112 LIVNYLPQDMTDRELYALFRTIGPINTCRIMRDYKTGYSFGYAFVDFAAETDSQRAIKSL 171

Query: 229 TGFNVCNRYICISYYQP--------NKVFKKVDYTAKNEELNNLKSKFGIGTPRNNVK*K 384
            G  V N+ + +SY +P        N     +  T  +++L+ +  K+G+   +N ++ K
Sbjct: 172 NGITVRNKRLKVSYARPGGESIKDTNLYVTNLPRTITDDQLDTIFGKYGMIVQKNILRDK 231

Query: 385 V 387
           +
Sbjct: 232 L 232

 Score = 39.7 bits (91), Expect = 0.003
 Identities = 25/60 (41%), Positives = 34/60 (56%), Gaps = 3/60 (5%)
 Frame = +1

Query: 58  LMVSNLPYELRDDEMYDIFGKYGTIRQ---IRVGNTTENKGKAFVVYEDIYDAQNACEHL 228
           L V+NLP  + DD++  IFGKYG I Q   +R   T + +G AFV +    +AQ A   L
Sbjct: 198 LYVTNLPRTITDDQLDTIFGKYGMIVQKNILRDKLTGKPRGVAFVRFNKREEAQEAISAL 257
>sp|P19339|SXL_DROME Sex-lethal protein
          Length = 354

 Score = 47.4 bits (111), Expect = 1e-05
 Identities = 34/121 (28%), Positives = 60/121 (49%), Gaps = 11/121 (9%)
 Frame = +1

Query: 58  LMVSNLPYELRDDEMYDIF---GKYGTIRQIRVGNTTENKGKAFVVYEDIYDAQNACEHL 228
           L+V+ LP ++ D E+Y +F   G   T R +R   T  + G AFV +    D+Q A + L
Sbjct: 127 LIVNYLPQDMTDRELYALFRAIGPINTCRIMRDYKTGYSFGYAFVDFTSEMDSQRAIKVL 186

Query: 229 TGFNVCNRYICISYYQP--------NKVFKKVDYTAKNEELNNLKSKFGIGTPRNNVK*K 384
            G  V N+ + +SY +P        N     +  T  +++L+ +  K+G    +N ++ K
Sbjct: 187 NGITVRNKRLKVSYARPGGESIKDTNLYVTNLPRTITDDQLDTIFGKYGSIVQKNILRDK 246

Query: 385 V 387
           +
Sbjct: 247 L 247

 Score = 41.6 bits (96), Expect = 8e-04
 Identities = 26/60 (43%), Positives = 34/60 (56%), Gaps = 3/60 (5%)
 Frame = +1

Query: 58  LMVSNLPYELRDDEMYDIFGKYGTIRQ---IRVGNTTENKGKAFVVYEDIYDAQNACEHL 228
           L V+NLP  + DD++  IFGKYG+I Q   +R   T   +G AFV Y    +AQ A   L
Sbjct: 213 LYVTNLPRTITDDQLDTIFGKYGSIVQKNILRDKLTGRPRGVAFVRYNKREEAQEAISAL 272
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 50,294,724
Number of Sequences: 369166
Number of extensions: 978234
Number of successful extensions: 3223
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3070
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3200
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2344429560
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)