Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_C20
(883 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9PPP0|SYE_UREPA Glutamyl-tRNA synthetase (Glutamate--tR... 39 0.025
sp|P28741|KIF3A_MOUSE Kinesin-like protein KIF3A (Microtubu... 34 0.62
sp|Q9Y496|KIF3A_HUMAN Kinesin-like protein KIF3A (Microtubu... 34 0.62
sp|P81412|DP2S_PYRFU DNA polymerase II small subunit (Pol I... 33 0.80
sp|P14732|LAM2_CHICK Lamin B2 32 1.8
sp|O51604|ERA_BORBU GTP-binding protein era homolog 32 1.8
sp|P25357|SNT1_YEAST Probable DNA-binding protein SNT1 32 2.3
sp|Q8K985|MDLA_BUCAP Multidrug resistance-like ATP-binding ... 32 2.3
sp|Q8N3U4|STAG2_HUMAN Cohesin subunit SA-2 (Stromal antigen... 32 3.1
sp|Q58969|Y1574_METJA Putative ATP-dependent helicase MJ1574 31 4.0
>sp|Q9PPP0|SYE_UREPA Glutamyl-tRNA synthetase (Glutamate--tRNA ligase) (GluRS)
Length = 482
Score = 38.5 bits (88), Expect = 0.025
Identities = 37/122 (30%), Positives = 59/122 (48%), Gaps = 3/122 (2%)
Frame = +3
Query: 240 KHDMLKLVPLPSHDYPIHNKEILFKITKERLNLKLDFFGIQFLYDEEVEAEDIENDKKEQ 419
KH + K + L +DY NK +LFK L +D QF D++V A D++ KK Q
Sbjct: 343 KHSLTKELVL--NDYTFINKCLLFKSHIINLKQLIDLVIEQFNCDKKVLASDVDYIKKNQ 400
Query: 420 KRNSARLYIE-IYDNDGKMKQFYKSPIIQKKDLDPLAQKSVVGNKG--IYSNRKITSSSN 590
R++ E + ND ++F K II+K + + NKG +Y +I ++ +
Sbjct: 401 LITVVRVFYEQLIINDEFNEEFIKE-IIKKVQI-------ITNNKGANLYMPIRIATTFS 452
Query: 591 MH 596
H
Sbjct: 453 SH 454
>sp|P28741|KIF3A_MOUSE Kinesin-like protein KIF3A (Microtubule plus end-directed kinesin
motor 3A)
Length = 701
Score = 33.9 bits (76), Expect = 0.62
Identities = 25/85 (29%), Positives = 42/85 (49%), Gaps = 4/85 (4%)
Frame = +3
Query: 357 IQFLYDEEVEAEDIENDKKEQKRNSARLYIEIYDNDGKMKQFYKSPIIQKKDLDPLAQKS 536
+Q DEE +A + + D +E++RN AR +E + D Q +++K L L +K
Sbjct: 424 MQAKIDEERKALETKLDMEEEERNKARAELERREKDLLKAQQEHQSLLEK--LSALEKKV 481
Query: 537 VVGNKGIYS----NRKITSSSNMHL 599
+VG + + K+ SNM L
Sbjct: 482 IVGGVDLLAKAEEQEKLLEESNMEL 506
>sp|Q9Y496|KIF3A_HUMAN Kinesin-like protein KIF3A (Microtubule plus end-directed kinesin
motor 3A)
Length = 702
Score = 33.9 bits (76), Expect = 0.62
Identities = 25/85 (29%), Positives = 42/85 (49%), Gaps = 4/85 (4%)
Frame = +3
Query: 357 IQFLYDEEVEAEDIENDKKEQKRNSARLYIEIYDNDGKMKQFYKSPIIQKKDLDPLAQKS 536
+Q DEE +A + + D +E++RN AR +E + D Q +++K L L +K
Sbjct: 425 MQAKIDEERKALETKLDMEEEERNKARAELEKREKDLLKAQQEHQSLLEK--LSALEKKV 482
Query: 537 VVGNKGIYS----NRKITSSSNMHL 599
+VG + + K+ SNM L
Sbjct: 483 IVGGVDLLAKAEEQEKLLEESNMEL 507
>sp|P81412|DP2S_PYRFU DNA polymerase II small subunit (Pol II) (DP1)
Length = 613
Score = 33.5 bits (75), Expect = 0.80
Identities = 23/76 (30%), Positives = 35/76 (46%)
Frame = +3
Query: 372 DEEVEAEDIENDKKEQKRNSARLYIEIYDNDGKMKQFYKSPIIQKKDLDPLAQKSVVGNK 551
DE V + N K+E + L EI D GK+K F ++ + +VV K
Sbjct: 258 DETVTIIGLVNSKREVNKG---LIFEIEDLTGKVKVFLPKDSEDYREAFKVLPDAVVAFK 314
Query: 552 GIYSNRKITSSSNMHL 599
G+YS R I ++ +L
Sbjct: 315 GVYSKRGILYANKFYL 330
>sp|P14732|LAM2_CHICK Lamin B2
Length = 600
Score = 32.3 bits (72), Expect = 1.8
Identities = 24/77 (31%), Positives = 39/77 (50%)
Frame = +3
Query: 288 IHNKEILFKITKERLNLKLDFFGIQFLYDEEVEAEDIENDKKEQKRNSARLYIEIYDNDG 467
+ N +L KI+++ + GI+ LY+ E+ D E + ARL IEI
Sbjct: 48 LENDRLLVKISEKEEVTTREVSGIKNLYESELA--DARRVLDETAKERARLQIEIGKLRA 105
Query: 468 KMKQFYKSPIIQKKDLD 518
++++F KS +KKD D
Sbjct: 106 ELEEFNKS--YKKKDAD 120
>sp|O51604|ERA_BORBU GTP-binding protein era homolog
Length = 290
Score = 32.3 bits (72), Expect = 1.8
Identities = 42/209 (20%), Positives = 95/209 (45%), Gaps = 16/209 (7%)
Frame = +3
Query: 210 ISVIGPDGDDKHDMLKLVPLPSHDYPIHNKEILFKITKERLNLKLDFFG--IQFLYDEEV 383
I + G++++ ML++ I N +I F + +++LK QFL ++ +
Sbjct: 90 IDIQDKPGEEENKMLEI---------IKNSKIKFLVILNKIDLKNTKIKEITQFLKEKGI 140
Query: 384 EAEDIENDKKEQKRNSARLYIEIYDN--DGKM---KQFYKSPIIQKKDLDPLAQKSVVGN 548
E +I E+K N+ L +IY+N +G + +++Y I + + + +K++
Sbjct: 141 EDSNIIKISAEKKINTEELKNKIYENFSEGPLYYPQEYYTDQEINFRISEIIREKAIENL 200
Query: 549 K-----GIYSNRKITSSSNMHLFFRSDVWI--KSYSGFCLVINHAKLQCYQD--IHDIRK 701
K +Y + + LF R+++++ +S G + N +++ + I K
Sbjct: 201 KEELPYSLYVDIDTLENKKGSLFIRANIFVANESQKGIIVGKNGKEIKSIGERARKTIAK 260
Query: 702 LFSGKLHYIFQRFRFTTTQNLTDTISQKL 788
+F K + +F + + N D + ++L
Sbjct: 261 IFETKCN-LFLQVKLKKNWNKEDKLIKRL 288
>sp|P25357|SNT1_YEAST Probable DNA-binding protein SNT1
Length = 1226
Score = 32.0 bits (71), Expect = 2.3
Identities = 20/55 (36%), Positives = 31/55 (56%), Gaps = 14/55 (25%)
Frame = +3
Query: 300 EILFKITKE----RLNLKLDFFGIQFLYDEEVE----------AEDIENDKKEQK 422
+I+ KI KE ++NLK D+F +Q Y++E E E+IEN +KE +
Sbjct: 517 KIISKIKKEEHLKKINLKHDYFDLQKKYEKECEILTKLSENLRKEEIENKRKEHE 571
>sp|Q8K985|MDLA_BUCAP Multidrug resistance-like ATP-binding protein mdlA
Length = 581
Score = 32.0 bits (71), Expect = 2.3
Identities = 20/58 (34%), Positives = 31/58 (53%), Gaps = 2/58 (3%)
Frame = +3
Query: 186 IYLEHNA--SISVIGPDGDDKHDMLKLVPLPSHDYPIHNKEILFKITKERLNLKLDFF 353
IYL N ++ + GP G K +LKL+ + IH EIL+ + L LK+D++
Sbjct: 355 IYLSLNPGKTLGICGPTGAGKSTLLKLI---QRQFKIHKGEILYN-SSSLLELKIDYW 408
>sp|Q8N3U4|STAG2_HUMAN Cohesin subunit SA-2 (Stromal antigen 2) (SCC3 homolog 2)
Length = 1231
Score = 31.6 bits (70), Expect = 3.1
Identities = 27/117 (23%), Positives = 53/117 (45%), Gaps = 1/117 (0%)
Frame = +3
Query: 18 MEPSVSSENSMEDSNNKIETNLNQN-QPVFFRRYYEDSKLNLSICSDENPIWVEFPEIYL 194
+E + ++++ ++IE +N + VF RY + +IC +E IW++
Sbjct: 264 LELLLQKRKELQENQDEIENMMNAIFKGVFVHRYRDAIAEIRAICIEEIGIWMKMYSDAF 323
Query: 195 EHNASISVIGPDGDDKHDMLKLVPLPSHDYPIHNKEILFKITKERLNLKLDFFGIQF 365
+++ + +G DK ++L L + +NKE LN KL+ F +F
Sbjct: 324 LNDSYLKYVGWTMHDKQGEVRLKCLTALQGLYYNKE---------LNSKLELFTSRF 371
>sp|Q58969|Y1574_METJA Putative ATP-dependent helicase MJ1574
Length = 684
Score = 31.2 bits (69), Expect = 4.0
Identities = 39/195 (20%), Positives = 77/195 (39%), Gaps = 31/195 (15%)
Frame = +3
Query: 246 DMLKLVPLPSHDYPIHNKEILFKITKERLNLKLDFFGIQFLYDEEVEAEDIENDKKEQ-- 419
+++K+ +P N FK + +N +D G + LY +V+A +KK+
Sbjct: 11 EIIKIYKIPERKGRFSN----FKFKNKEINELIDALGFK-LYLHQVKALKYLYNKKDVVV 65
Query: 420 -------KRNSARL-------------YIEIYDNDGKMKQFYKSPIIQKKDLDPLAQKSV 539
K RL Y+ IY + Y+ ++ + + K V
Sbjct: 66 TTSTASGKSEIFRLAIFDNFLSNPDDRYLLIYPTRALINNQYEKFSMENELFYKITNKRV 125
Query: 540 -----VGNKGIYSNRKITSSSNMHLFFRSDVW----IKSYSGFCLVINHAKLQCYQDIHD 692
G+ G+ R+I LF D+ +K+++ + ++ + KL ++H
Sbjct: 126 KAEILTGDVGLEKRREILKDKPNVLFTTPDMLHYQILKNHNNYLWLLKNLKLLVVDELHV 185
Query: 693 IRKLFSGKLHYIFQR 737
R +F + Y+F+R
Sbjct: 186 YRGVFGTNMVYVFKR 200
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 103,247,281
Number of Sequences: 369166
Number of extensions: 2204931
Number of successful extensions: 6364
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6094
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6354
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8790245790
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)