Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_C03
(775 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinas... 108 2e-23
sp|Q05397|FAK1_HUMAN Focal adhesion kinase 1 (FADK 1) (pp12... 102 2e-21
sp|O35346|FAK1_RAT Focal adhesion kinase 1 (FADK 1) (pp125FAK) 102 2e-21
sp|P34152|FAK1_MOUSE Focal adhesion kinase 1 (FADK 1) (pp12... 102 2e-21
sp|Q9SZ67|WRK19_ARATH Probable WRKY transcription factor 19... 101 3e-21
sp|Q91738|FAK1_XENLA Focal adhesion kinase 1 (FADK 1) (pp12... 100 3e-21
sp|Q00944|FAK1_CHICK Focal adhesion kinase 1 (FADK 1) (PP12... 99 1e-20
sp|Q12236|KOK0_YEAST Probable serine/threonine-protein kina... 99 2e-20
sp|P35465|PAK1_RAT Serine/threonine-protein kinase PAK 1 (p... 99 2e-20
sp|P42687|SPK1_DUGTI Tyrosine-protein kinase SPK-1 98 2e-20
>sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinase kinase 5 (MAPK/ERK kinase
kinase 5) (MEK kinase 5) (MEKK 5) (Apoptosis
signal-regulating kinase 1) (ASK-1)
Length = 1374
Score = 108 bits (269), Expect = 2e-23
Identities = 68/220 (30%), Positives = 113/220 (51%), Gaps = 7/220 (3%)
Frame = +3
Query: 78 LKELITFQMTKSHRRFIQLLGVYIDDAILYVVMENMDRGTLKTFLQENYKNLDKNK--IY 251
L E I H+ +Q LG + ++ + + ME + G+L L+ + L N+ I
Sbjct: 722 LHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIG 781
Query: 252 KWSAQILEGLKFLHENKVIHRDLNPSSILVDGNED-LKLSSFNLSKYL-DISKAPASIMG 425
++ QILEGLK+LH+N+++HRD+ ++L++ LK+S F SK L I+ + G
Sbjct: 782 FYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTG 841
Query: 426 KHDKKKELPLRYMAPEILNSD--NYGEACDVWSFGAVLIRITTNKKPYSNLNKSEALDHI 599
L+YMAPEI++ YG+A D+WS G +I + T K P+ L + +A
Sbjct: 842 --------TLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFK 893
Query: 600 SKLKMIEYKLPNKYS-EFKSIINLCLQQDPSWRGKVKELI 716
+ + ++P S E K+ I C + DP R +L+
Sbjct: 894 VGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLL 933
>sp|Q05397|FAK1_HUMAN Focal adhesion kinase 1 (FADK 1) (pp125FAK) (Protein-tyrosine kinase
2)
Length = 1052
Score = 102 bits (253), Expect = 2e-21
Identities = 63/225 (28%), Positives = 116/225 (51%), Gaps = 1/225 (0%)
Frame = +3
Query: 69 EFMLKELITFQMTKSHRRFIQLLGVYIDDAILYVVMENMDRGTLKTFLQENYKNLDKNKI 248
E L+E +T + H ++L+GV ++ + +++ME G L++FLQ +LD +
Sbjct: 466 EKFLQEALTMRQF-DHPHIVKLIGVITENPV-WIIMELCTLGELRSFLQVRKYSLDLASL 523
Query: 249 YKWSAQILEGLKFLHENKVIHRDLNPSSILVDGNEDLKLSSFNLSKYLDISKAPASIMGK 428
++ Q+ L +L + +HRD+ ++LV N+ +KL F LS+Y++ S + GK
Sbjct: 524 ILYAYQLSTALAYLESKRFVHRDIAARNVLVSSNDCVKLGDFGLSRYMEDSTYYKASKGK 583
Query: 429 HDKKKELPLRYMAPEILNSDNYGEACDVWSFGAVLIRITTN-KKPYSNLNKSEALDHISK 605
LP+++MAPE +N + A DVW FG + I + KP+ + ++ + I
Sbjct: 584 ------LPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIEN 637
Query: 606 LKMIEYKLPNKYSEFKSIINLCLQQDPSWRGKVKELIEKINNVLK 740
+ + PN S++ C DPS R + EL +++ +L+
Sbjct: 638 GERLPMP-PNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILE 681
>sp|O35346|FAK1_RAT Focal adhesion kinase 1 (FADK 1) (pp125FAK)
Length = 1055
Score = 102 bits (253), Expect = 2e-21
Identities = 63/225 (28%), Positives = 116/225 (51%), Gaps = 1/225 (0%)
Frame = +3
Query: 69 EFMLKELITFQMTKSHRRFIQLLGVYIDDAILYVVMENMDRGTLKTFLQENYKNLDKNKI 248
E L+E +T + H ++L+GV ++ + +++ME G L++FLQ +LD +
Sbjct: 466 EKFLQEALTMRQF-DHPHIVKLIGVITENPV-WIIMELCTLGELRSFLQVRKYSLDLASL 523
Query: 249 YKWSAQILEGLKFLHENKVIHRDLNPSSILVDGNEDLKLSSFNLSKYLDISKAPASIMGK 428
++ Q+ L +L + +HRD+ ++LV N+ +KL F LS+Y++ S + GK
Sbjct: 524 ILYAYQLSTALAYLESKRFVHRDIAARNVLVSSNDCVKLGDFGLSRYMEDSTYYKASKGK 583
Query: 429 HDKKKELPLRYMAPEILNSDNYGEACDVWSFGAVLIRITTN-KKPYSNLNKSEALDHISK 605
LP+++MAPE +N + A DVW FG + I + KP+ + ++ + I
Sbjct: 584 ------LPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIEN 637
Query: 606 LKMIEYKLPNKYSEFKSIINLCLQQDPSWRGKVKELIEKINNVLK 740
+ + PN S++ C DPS R + EL +++ +L+
Sbjct: 638 GERLPMP-PNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILE 681
>sp|P34152|FAK1_MOUSE Focal adhesion kinase 1 (FADK 1) (pp125FAK)
Length = 1090
Score = 102 bits (253), Expect = 2e-21
Identities = 63/225 (28%), Positives = 116/225 (51%), Gaps = 1/225 (0%)
Frame = +3
Query: 69 EFMLKELITFQMTKSHRRFIQLLGVYIDDAILYVVMENMDRGTLKTFLQENYKNLDKNKI 248
E L+E +T + H ++L+GV ++ + +++ME G L++FLQ +LD +
Sbjct: 504 EKFLQEALTMRQF-DHPHIVKLIGVITENPV-WIIMELCTLGELRSFLQVRKYSLDLASL 561
Query: 249 YKWSAQILEGLKFLHENKVIHRDLNPSSILVDGNEDLKLSSFNLSKYLDISKAPASIMGK 428
++ Q+ L +L + +HRD+ ++LV N+ +KL F LS+Y++ S + GK
Sbjct: 562 ILYAYQLSTALAYLESKRFVHRDIAARNVLVSSNDCVKLGDFGLSRYMEDSTYYKASKGK 621
Query: 429 HDKKKELPLRYMAPEILNSDNYGEACDVWSFGAVLIRITTN-KKPYSNLNKSEALDHISK 605
LP+++MAPE +N + A DVW FG + I + KP+ + ++ + I
Sbjct: 622 ------LPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIEN 675
Query: 606 LKMIEYKLPNKYSEFKSIINLCLQQDPSWRGKVKELIEKINNVLK 740
+ + PN S++ C DPS R + EL +++ +L+
Sbjct: 676 GERLPMP-PNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILE 719
>sp|Q9SZ67|WRK19_ARATH Probable WRKY transcription factor 19 (WRKY DNA-binding protein 19)
Length = 1895
Score = 101 bits (251), Expect = 3e-21
Identities = 62/206 (30%), Positives = 110/206 (53%), Gaps = 5/206 (2%)
Frame = +3
Query: 114 HRRFIQLLGVYIDDAILYVVMENMDRGTLKTFLQENYKNLDKNKIYKWSAQILEGLKFLH 293
H+ ++ G D++ LY+ +E + +G+L+ Q N L + + ++ QIL+GLK+LH
Sbjct: 1684 HQNIVRYRGTTKDESNLYIFLELVTQGSLRKLYQRN--QLGDSVVSLYTRQILDGLKYLH 1741
Query: 294 ENKVIHRDLNPSSILVDGNEDLKLSSFNLSKYLDISKAPASIMGKHDKKKELPLRYMAPE 473
+ IHR++ +++LVD N +KL+ F L+K + + + P +MAPE
Sbjct: 1742 DKGFIHRNIKCANVLVDANGTVKLADFGLAKVMSLWRTPY-------------WNWMAPE 1788
Query: 474 IL----NSDNYGEACDVWSFGAVLIRITTNKKPYSNLNKSEALDHISKLKMIEYKLPNKY 641
++ + D YG D+WS G ++ + T + PYS+L AL +I K+ K+P+
Sbjct: 1789 VILNPKDYDGYGTPADIWSLGCTVLEMLTGQIPYSDLEIGTALYNIGTGKL--PKIPDIL 1846
Query: 642 S-EFKSIINLCLQQDPSWRGKVKELI 716
S + + I CL+ +P R EL+
Sbjct: 1847 SLDARDFILTCLKVNPEERPTAAELL 1872
>sp|Q91738|FAK1_XENLA Focal adhesion kinase 1 (FADK 1) (pp125FAK)
Length = 1068
Score = 100 bits (250), Expect = 3e-21
Identities = 63/225 (28%), Positives = 115/225 (51%), Gaps = 1/225 (0%)
Frame = +3
Query: 69 EFMLKELITFQMTKSHRRFIQLLGVYIDDAILYVVMENMDRGTLKTFLQENYKNLDKNKI 248
E L+E +T + H ++L+GV ++ + +++ME G L++FLQ +LD +
Sbjct: 479 EKFLQEALTMRQF-DHPHIVKLIGVITENPV-WIIMELCTLGELRSFLQVRKYSLDLASL 536
Query: 249 YKWSAQILEGLKFLHENKVIHRDLNPSSILVDGNEDLKLSSFNLSKYLDISKAPASIMGK 428
+S Q+ L +L + +HRD+ ++LV ++ +KL F LS+Y++ S + GK
Sbjct: 537 ILYSYQLSTALAYLESKRFVHRDIAARNVLVSSSDCVKLGDFGLSRYMEDSTYYKASKGK 596
Query: 429 HDKKKELPLRYMAPEILNSDNYGEACDVWSFGAVLIRITT-NKKPYSNLNKSEALDHISK 605
LP+++MAPE +N + A DVW FG + I KP+ + ++ + I
Sbjct: 597 ------LPIKWMAPESINFRRFTSASDVWMFGVCMWEILMYGVKPFQGVKNNDVIGRIEN 650
Query: 606 LKMIEYKLPNKYSEFKSIINLCLQQDPSWRGKVKELIEKINNVLK 740
+ + PN S++ C DPS R + EL +++ +L+
Sbjct: 651 GERLPMP-PNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILE 694
>sp|Q00944|FAK1_CHICK Focal adhesion kinase 1 (FADK 1) (PP125FAK)
Length = 1053
Score = 99.4 bits (246), Expect = 1e-20
Identities = 62/225 (27%), Positives = 115/225 (51%), Gaps = 1/225 (0%)
Frame = +3
Query: 69 EFMLKELITFQMTKSHRRFIQLLGVYIDDAILYVVMENMDRGTLKTFLQENYKNLDKNKI 248
E L+E +T + H ++L+GV ++ + +++ME G L++FLQ +LD +
Sbjct: 466 EKFLQEALTMRQF-DHPHIVKLIGVITENPV-WIIMELCTLGELRSFLQVRKFSLDLASL 523
Query: 249 YKWSAQILEGLKFLHENKVIHRDLNPSSILVDGNEDLKLSSFNLSKYLDISKAPASIMGK 428
++ Q+ L +L + +HRD+ ++LV + +KL F LS+Y++ S + GK
Sbjct: 524 ILYAYQLSTALAYLESKRFVHRDIAARNVLVSATDCVKLGDFGLSRYMEDSTYYKASKGK 583
Query: 429 HDKKKELPLRYMAPEILNSDNYGEACDVWSFGAVLIRITTN-KKPYSNLNKSEALDHISK 605
LP+++MAPE +N + A DVW FG + I + KP+ + ++ + I
Sbjct: 584 ------LPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIEN 637
Query: 606 LKMIEYKLPNKYSEFKSIINLCLQQDPSWRGKVKELIEKINNVLK 740
+ + PN S++ C DPS R + EL +++ +L+
Sbjct: 638 GERLPMP-PNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILE 681
>sp|Q12236|KOK0_YEAST Probable serine/threonine-protein kinase YOL100W
Length = 1081
Score = 98.6 bits (244), Expect = 2e-20
Identities = 64/244 (26%), Positives = 116/244 (47%), Gaps = 5/244 (2%)
Frame = +3
Query: 9 NTEILIRKYEVKSSKTWKDEEFMLKELITFQMTKSHRRFIQLLGVYIDDAILYVVMENMD 188
N E LIR+ +VK ++ E Q + ++L + D++ LY ++E
Sbjct: 211 NKEYLIRQKKVK---------YVSIEKTALQKLNNSPSVVRLFSTFQDESSLYFLLEYAP 261
Query: 189 RGTLKTFLQENYKNLDKNKIYKWSAQILEGLKFLHENKVIHRDLNPSSILVDGNEDLKLS 368
G + L + Y +LD+ ++AQI++ + +LH N +IHRD+ P +IL+DG +KL+
Sbjct: 262 NGDFLS-LMKKYGSLDETCARYYAAQIIDAIDYLHSNGIIHRDIKPENILLDGEMKIKLT 320
Query: 369 SFNLSKYLD-----ISKAPASIMGKHDKKKELPLRYMAPEILNSDNYGEACDVWSFGAVL 533
F +K L+ +SK P + K Y++PE+LN CD+W+FG +L
Sbjct: 321 DFGTAKLLNPTNNSVSK-PEYDLSTRSKSFVGTAEYVSPELLNDSFTDYRCDIWAFGCIL 379
Query: 534 IRITTNKKPYSNLNKSEALDHISKLKMIEYKLPNKYSEFKSIINLCLQQDPSWRGKVKEL 713
++ K P +K N+Y F+ ++ + P + +++L
Sbjct: 380 FQMIAGKPP--------------------FKATNEYLTFQKVMKVQYAFTPGFPLIIRDL 419
Query: 714 IEKI 725
++KI
Sbjct: 420 VKKI 423
>sp|P35465|PAK1_RAT Serine/threonine-protein kinase PAK 1 (p21-activated kinase 1)
(PAK-1) (P68-PAK) (Alpha-PAK) (Protein kinase MUK2)
Length = 544
Score = 98.6 bits (244), Expect = 2e-20
Identities = 68/239 (28%), Positives = 116/239 (48%), Gaps = 1/239 (0%)
Frame = +3
Query: 6 TNTEILIRKYEVKSSKTWKDEEFMLKELITFQMTKSHRRFIQLLGVYIDDAILYVVMENM 185
T E+ I++ ++ +E ++ E++ + K+ + L Y+ L+VVME +
Sbjct: 291 TGQEVAIKQMNLQQQPK---KELIINEILVMRENKNPN-IVNYLDSYLVGDELWVVMEYL 346
Query: 186 DRGTLKTFLQENYKNLDKNKIYKWSAQILEGLKFLHENKVIHRDLNPSSILVDGNEDLKL 365
G+L + E +D+ +I + L+ L+FLH N+VIHRD+ +IL+ + +KL
Sbjct: 347 AGGSLTDVVTETC--MDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKL 404
Query: 366 SSFNLSKYLDISKAPASIMGKHDKKKELPLRYMAPEILNSDNYGEACDVWSFGAVLIRIT 545
+ F + ++ S M +MAPE++ YG D+WS G + I +
Sbjct: 405 TDFGFCAQITPEQSKRSTMVGTPY-------WMAPEVVTRKAYGPKVDIWSLGIMAIEMI 457
Query: 546 TNKKPYSNLNKSEALDHISKLKMIEYKLPNKYSE-FKSIINLCLQQDPSWRGKVKELIE 719
+ PY N N AL I+ E + P K S F+ +N CL+ D RG KEL++
Sbjct: 458 EGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQ 516
>sp|P42687|SPK1_DUGTI Tyrosine-protein kinase SPK-1
Length = 497
Score = 98.2 bits (243), Expect = 2e-20
Identities = 77/253 (30%), Positives = 127/253 (50%), Gaps = 19/253 (7%)
Frame = +3
Query: 27 RKYEV-----KSSKTWKDEEFMLKELITFQMTKSHRRFIQLLGVYID--DAILYVVMENM 185
R YEV +SSK + E+F+L+ I ++ HR+ ++LLGV + D + +++E M
Sbjct: 241 RSYEVAAKMLQSSKAER-EKFVLEAKIMHKLR--HRKIVELLGVCTEPQDMPMLIIVEYM 297
Query: 186 DRGTLKTFLQE-NYKNLDKNKIYKWSAQILEGLKFLHENKVIHRDLNPSSILVDGNEDLK 362
G+LK +L+ + K + N++ A+I EG+ +L KV+HRDL +ILV + K
Sbjct: 298 KNGSLKEYLKTPDGKKTNLNQMVHMMAEISEGMAYLESEKVVHRDLRADNILVANDLTRK 357
Query: 363 LSSFNLSKYLDISKAPASIMGKHDKKK-ELPLRYMAPEILNSDNYGEACDVWSFGAVLIR 539
++ F L++ D S +G +KK P ++ APE S + DVWS+G V+
Sbjct: 358 VADFGLTELTDGS------LGDQEKKTLRFPYKWTAPEAAKSKVFTSKSDVWSYGIVMFE 411
Query: 540 ITT-NKKPYSNLNKSEALDHISKLKMIEYKLPNKY---------SEFKSIINLCLQQDPS 689
I T PY ++ E ++ +SK Y++PN E I+ C +P
Sbjct: 412 ILTWASSPYPDIPAKEVIEKVSK----GYRMPNPEKFITGVCCPDEIYKIMIWCWDANPE 467
Query: 690 WRGKVKELIEKIN 728
R L EK++
Sbjct: 468 KRPTFLVLQEKMD 480
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,884,784
Number of Sequences: 369166
Number of extensions: 1683430
Number of successful extensions: 7836
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6065
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6426
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7212136400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)