Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_B24
(890 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O15294|OGT1_HUMAN UDP-N-acetylglucosamine--peptide N-ace... 305 1e-82
sp|P56558|OGT1_RAT UDP-N-acetylglucosamine--peptide N-acety... 300 3e-81
sp|O18158|OGT1_CAEEL UDP-N-acetylglucosamine--peptide N-ace... 276 6e-74
sp|Q9M8Y0|SEC_ARATH Probable UDP-N-acetylglucosamine--pepti... 173 5e-43
sp|O82422|SPY_HORVU Probable UDP-N-acetylglucosamine--pepti... 93 1e-18
sp|Q8RVB2|SPY_LYCES Probable UDP-N-acetylglucosamine--pepti... 91 4e-18
sp|O82039|SPY_PETHY Probable UDP-N-acetylglucosamine--pepti... 91 4e-18
sp|Q6YZI0|SPY_ORYSA Probable UDP-N-acetylglucosamine--pepti... 87 5e-17
sp|Q96301|SPY_ARATH Probable UDP-N-acetylglucosamine--pepti... 85 3e-16
sp|Q8LP10|SPY_EUSGR Probable UDP-N-acetylglucosamine--pepti... 84 7e-16
>sp|O15294|OGT1_HUMAN UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110
kDa subunit (O-GlcNAc transferase p110 subunit)
Length = 1046
Score = 305 bits (780), Expect = 1e-82
Identities = 151/268 (56%), Positives = 200/268 (74%), Gaps = 3/268 (1%)
Frame = +2
Query: 5 SVPVVVIDSFTEINDMIVNKREFA-TINGVTAANGMAITETVKKFS-GEKIINQIIITSR 178
++PV+ +++ E ++N+ + TING + +NG+A T+ K + GE++ II+T+R
Sbjct: 773 NMPVIPMNTIAEAVIEMINRGQIQITINGFSISNGLATTQINNKAATGEEVPRTIIVTTR 832
Query: 179 KHYNLPDNATVFCNFNQLYKIGPDTLKMWVKILQNCPKSVLWLLRFPAAGEKNVYKAFTD 358
Y LP++A V+CNFNQLYKI P TL+MW IL+ P SVLWLLRFPA GE N+ + +
Sbjct: 833 SQYGLPEDAIVYCNFNQLYKIDPSTLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQN 892
Query: 359 FGLKDVHSRVIFSDVAPKEEHVRRGQVADVCLDTPLCNGHTTGMDVLWSGCPMVTLPAET 538
GL +R+IFS VAPKEEHVRRGQ+ADVCLDTPLCNGHTTGMDVLW+G PMVT+P ET
Sbjct: 893 MGLPQ--NRIIFSPVAPKEEHVRRGQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGET 950
Query: 539 LASRVAASQLNTLGCPELIAKSEEDYVNIAVRLGNEPKYLESVRNKVWKNRISSPLFNCQ 718
LASRVAASQL LGC ELIAK+ ++Y +IAV+LG + +YL+ VR KVWK RISSPLFN +
Sbjct: 951 LASRVAASQLTCLGCLELIAKNRQEYEDIAVKLGTDLEYLKKVRGKVWKQRISSPLFNTK 1010
Query: 719 LYTRSLESLYQEMWNNF-SNDRFDHITR 799
YT LE LY +MW ++ + ++ DH+ +
Sbjct: 1011 QYTMELERLYLQMWEHYAAGNKPDHMIK 1038
>sp|P56558|OGT1_RAT UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110
kDa subunit (O-GlcNAc transferase p110 subunit)
Length = 1036
Score = 300 bits (769), Expect = 3e-81
Identities = 150/268 (55%), Positives = 199/268 (74%), Gaps = 3/268 (1%)
Frame = +2
Query: 5 SVPVVVIDSFTEINDMIVNKREFA-TINGVTAANGMAITETVKKFS-GEKIINQIIITSR 178
++PV+ +++ E ++N+ + TING + +NG+A T+ K + GE++ II+T+R
Sbjct: 763 NMPVIPMNTIAEAVIEMINRGQIQITINGFSISNGLATTQINNKAATGEEVPRTIIVTTR 822
Query: 179 KHYNLPDNATVFCNFNQLYKIGPDTLKMWVKILQNCPKSVLWLLRFPAAGEKNVYKAFTD 358
Y LP++A V+CNFNQLYKI P TL+M IL+ P SVLWLLRFPA GE N+ + +
Sbjct: 823 SQYGLPEDAIVYCNFNQLYKIDPSTLQMGANILKRVPNSVLWLLRFPAVGEPNIQQYAQN 882
Query: 359 FGLKDVHSRVIFSDVAPKEEHVRRGQVADVCLDTPLCNGHTTGMDVLWSGCPMVTLPAET 538
GL +R+IFS VAPKEEHVRRGQ+ADVCLDTPLCNGHTTGMDVLW+G PMVT+P ET
Sbjct: 883 MGLPQ--NRIIFSPVAPKEEHVRRGQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGET 940
Query: 539 LASRVAASQLNTLGCPELIAKSEEDYVNIAVRLGNEPKYLESVRNKVWKNRISSPLFNCQ 718
LASRVAASQL LGC ELIAKS ++Y +IAV+LG + +YL+ +R KVWK RISSPLFN +
Sbjct: 941 LASRVAASQLTCLGCLELIAKSRQEYEDIAVKLGTDLEYLKKIRGKVWKQRISSPLFNTK 1000
Query: 719 LYTRSLESLYQEMWNNF-SNDRFDHITR 799
YT LE LY +MW ++ + ++ DH+ +
Sbjct: 1001 QYTMELERLYLQMWEHYAAGNKPDHMIK 1028
>sp|O18158|OGT1_CAEEL UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase
(O-GlcNAc) (OGT)
Length = 1151
Score = 276 bits (706), Expect = 6e-74
Identities = 134/224 (59%), Positives = 169/224 (75%), Gaps = 1/224 (0%)
Frame = +2
Query: 128 KKFSGEKIINQIIITSRKHYNLPDNATVFCNFNQLYKIGPDTLKMWVKILQNCPKSVLWL 307
K +GE+I N +++TSR Y LPD+A VFCNFNQLYKI P TL MW+KIL+N PKS+LWL
Sbjct: 921 KAATGEEIPNSVLLTSRAQYQLPDDAIVFCNFNQLYKIDPSTLDMWIKILENVPKSILWL 980
Query: 308 LRFPAAGEKNVYKAFTDFGLKDVHSRVIFSDVAPKEEHVRRGQVADVCLDTPLCNGHTTG 487
LRFP GE+++ K + GL SR++FS+VA KEEHVRRGQ+ADVCLDTPLCNGHTTG
Sbjct: 981 LRFPYQGEEHIRKYCVERGLDP--SRIVFSNVAAKEEHVRRGQLADVCLDTPLCNGHTTG 1038
Query: 488 MDVLWSGCPMVTLPAETLASRVAASQLNTLGCPELIAKSEEDYVNIAVRLGNEPKYLESV 667
MD+LW+G PMVT+P E+LASRVA SQL LG PEL+AK+ ++YV+IAVRLG + +L ++
Sbjct: 1039 MDILWTGTPMVTMPLESLASRVATSQLYALGVPELVAKTRQEYVSIAVRLGTDADHLANM 1098
Query: 668 RNKVWKNRISSPLFNCQLYTRSLESLYQEMWNNF-SNDRFDHIT 796
R KVW R SS LF+ + Y +E L +MW + S DHIT
Sbjct: 1099 RAKVWMARTSSTLFDVKQYCHDMEDLLGQMWKRYESGMPIDHIT 1142
>sp|Q9M8Y0|SEC_ARATH Probable UDP-N-acetylglucosamine--peptide
N-acetylglucosaminyltransferase SEC (Protein SECRET
AGENT)
Length = 977
Score = 173 bits (439), Expect = 5e-43
Identities = 92/198 (46%), Positives = 126/198 (63%), Gaps = 2/198 (1%)
Frame = +2
Query: 176 RKHYNLPDNATVFCNFNQLYKIGPDTLKMWVKILQNCPKSVLWLLRFPAAGEKNVYKAFT 355
R Y LP++ +F FNQLYK+ P+ + W IL+ P S LWLLRFPAAGE
Sbjct: 759 RSDYGLPEDKFIFACFNQLYKMDPEIVNTWCNILKRVPNSALWLLRFPAAGEMRFRTYAA 818
Query: 356 DFGLKDVHSRVIFSDVAPKEEHVRRGQVADVCLDTPLCNGHTTGMDVLWSGCPMVTLPAE 535
G++ ++IF+DVA K EH+RR +ADV LDTPLCNGHTTG DVLW+G PM+TLP E
Sbjct: 819 AQGVQP--DQIIFTDVAMKSEHIRRSVLADVILDTPLCNGHTTGTDVLWAGVPMITLPLE 876
Query: 536 TLASRVAASQLNTLGCPE-LIAKSEEDYVNIAVRLG-NEPKYLESVRNKVWKNRISSPLF 709
+A+RVA S G +I S E+Y AV L N+PK L+++ ++ +R++ PLF
Sbjct: 877 KMATRVAGSLCLATGLGHGMIVNSLEEYEEKAVSLALNKPK-LQALTKELRASRLTCPLF 935
Query: 710 NCQLYTRSLESLYQEMWN 763
+ + ++LE Y +MWN
Sbjct: 936 DTMRWVKNLERSYFKMWN 953
>sp|O82422|SPY_HORVU Probable UDP-N-acetylglucosamine--peptide
N-acetylglucosaminyltransferase SPINDLY (HvSPY)
Length = 944
Score = 92.8 bits (229), Expect = 1e-18
Identities = 60/201 (29%), Positives = 103/201 (51%), Gaps = 4/201 (1%)
Frame = +2
Query: 212 FCNFNQLYKIGPDTLKMWVKILQNCPKSVLWLLRFPAAGEKNVYKAFTDFGLKDVHSRVI 391
F +FN L KI P +++W +IL P S L + P + K + L+++ +
Sbjct: 648 FGSFNNLAKITPKVMQVWARILCAVPNSRLVVKCKPFCCDSIRQKFLST--LEELGLESL 705
Query: 392 FSDVAP----KEEHVRRGQVADVCLDTPLCNGHTTGMDVLWSGCPMVTLPAETLASRVAA 559
D+ P +H++ + D+ LDT G TT + L+ G P VT+ A V
Sbjct: 706 RVDLLPLIHLNHDHMQAYSLMDISLDTFPYAGTTTTCESLYMGVPCVTMAGSVHAHNVGV 765
Query: 560 SQLNTLGCPELIAKSEEDYVNIAVRLGNEPKYLESVRNKVWKNRISSPLFNCQLYTRSLE 739
S L +G L+AK+E++YV++A+ L ++ LE +R + + I SP+ + + +TR LE
Sbjct: 766 SLLTKVGLGRLVAKTEDEYVSLALDLASDVSALEELRKSLRELMIKSPVCDGESFTRGLE 825
Query: 740 SLYQEMWNNFSNDRFDHITRL 802
S Y+ MW+ + + + RL
Sbjct: 826 SAYRSMWHRYCDGDSPALRRL 846
>sp|Q8RVB2|SPY_LYCES Probable UDP-N-acetylglucosamine--peptide
N-acetylglucosaminyltransferase SPINDLY (LeSPY)
Length = 931
Score = 90.9 bits (224), Expect = 4e-18
Identities = 61/208 (29%), Positives = 104/208 (50%), Gaps = 4/208 (1%)
Frame = +2
Query: 191 LPDNATVFCNFNQLYKIGPDTLKMWVKILQNCPKSVLWLLRFPAAGEKNVYKAFTDFGLK 370
L + F +FN L KI P LK+W +IL P S L + P + +V + F L+
Sbjct: 655 LSNGFVTFGSFNNLAKITPKVLKVWARILSAVPHSRLIVKCKPFCCD-SVRQRFLSI-LE 712
Query: 371 DVHSRVIFSDVAP----KEEHVRRGQVADVCLDTPLCNGHTTGMDVLWSGCPMVTLPAET 538
+ D+ P +H++ + D+ LDT G TT + L+ G P VT+
Sbjct: 713 QLGLEPQRVDLLPLILLNHDHMQAYSLMDISLDTFPYAGTTTTCESLYMGVPCVTMGGSV 772
Query: 539 LASRVAASQLNTLGCPELIAKSEEDYVNIAVRLGNEPKYLESVRNKVWKNRISSPLFNCQ 718
A V S L T+G L+A++E++YV A++L ++ L ++R + + SPL +
Sbjct: 773 HAHNVGVSLLKTVGLENLVARNEDEYVESAIQLASDVTSLSNLRMSLRELMSKSPLCDGA 832
Query: 719 LYTRSLESLYQEMWNNFSNDRFDHITRL 802
+TR++ES+Y+ MW + + + R+
Sbjct: 833 KFTRNIESIYRSMWRRYCDGDVPSLRRM 860
>sp|O82039|SPY_PETHY Probable UDP-N-acetylglucosamine--peptide
N-acetylglucosaminyltransferase SPINDLY (PhSPY)
Length = 932
Score = 90.9 bits (224), Expect = 4e-18
Identities = 60/208 (28%), Positives = 106/208 (50%), Gaps = 4/208 (1%)
Frame = +2
Query: 191 LPDNATVFCNFNQLYKIGPDTLKMWVKILQNCPKSVLWLLRFPAAGEKNVYKAFTDFGLK 370
L + F +FN L KI P L++W +IL P S L +++ G +V + F L+
Sbjct: 655 LTNGFVTFGSFNNLAKITPKVLQVWARILCAVPHSRL-IVKCKPFGCDSVRQRFLSI-LE 712
Query: 371 DVHSRVIFSDVAP----KEEHVRRGQVADVCLDTPLCNGHTTGMDVLWSGCPMVTLPAET 538
+ D+ P +H++ + D+ LDT G TT + L+ G P VT+
Sbjct: 713 QLGLEPQRVDLVPLILLNHDHMQAYSLMDISLDTFPYAGTTTTCESLYMGVPCVTMGGSV 772
Query: 539 LASRVAASQLNTLGCPELIAKSEEDYVNIAVRLGNEPKYLESVRNKVWKNRISSPLFNCQ 718
A V S L T+G +L+A++E++YV +A++L ++ L ++R + + SPL +
Sbjct: 773 HAHNVGVSLLKTVGLRKLVARNEDEYVELAIQLASDVTSLSNLRMSLRELMAKSPLCDGA 832
Query: 719 LYTRSLESLYQEMWNNFSNDRFDHITRL 802
+T++LES Y+ MW + + + R+
Sbjct: 833 QFTQNLESTYRSMWRRYCDGDVPSLRRM 860
>sp|Q6YZI0|SPY_ORYSA Probable UDP-N-acetylglucosamine--peptide
N-acetylglucosaminyltransferase SPINDLY
Length = 927
Score = 87.4 bits (215), Expect = 5e-17
Identities = 61/202 (30%), Positives = 98/202 (48%), Gaps = 4/202 (1%)
Frame = +2
Query: 212 FCNFNQLYKIGPDTLKMWVKILQNCPKSVLWLLRFPAAGEKNVYKAFTDFGLKDVHSRVI 391
F +FN L KI P L++W KIL P S L + P + K + L ++ +
Sbjct: 648 FGSFNNLAKITPKVLQVWAKILCAVPNSRLVVKCKPFCCDSIRQKFLST--LAELGLEPL 705
Query: 392 FSDVAP----KEEHVRRGQVADVCLDTPLCNGHTTGMDVLWSGCPMVTLPAETLASRVAA 559
D+ P +H++ + D+ LDT G TT + L+ G P VT+ A V
Sbjct: 706 RVDLLPLIHLNHDHMQAYSLMDISLDTFPYAGTTTTCESLYMGVPCVTMAGSVHAHNVGV 765
Query: 560 SQLNTLGCPELIAKSEEDYVNIAVRLGNEPKYLESVRNKVWKNRISSPLFNCQLYTRSLE 739
S L +G L+AKSE +YV++A+ L + L+ +R + SP+ + + +TR LE
Sbjct: 766 SLLTKVGLGRLVAKSENEYVSLALDLAADVTALQELRMSLRGLMAKSPVCDGENFTRGLE 825
Query: 740 SLYQEMWNNFSNDRFDHITRLN 805
S Y+ MW + + + RL+
Sbjct: 826 SAYRNMWRRYCDGDAPALRRLD 847
>sp|Q96301|SPY_ARATH Probable UDP-N-acetylglucosamine--peptide
N-acetylglucosaminyltransferase SPINDLY
Length = 914
Score = 84.7 bits (208), Expect = 3e-16
Identities = 58/208 (27%), Positives = 95/208 (45%), Gaps = 4/208 (1%)
Frame = +2
Query: 191 LPDNATVFCNFNQLYKIGPDTLKMWVKILQNCPKSVLWLLRFPAAGEKNVYKAFTDFGLK 370
L + F +FN L KI P L++W +IL P S L + P + + T
Sbjct: 650 LSNGFVTFGSFNNLAKITPKVLQVWARILCAVPNSRLVVKCKPFCCDSIRQRFLTTLEQL 709
Query: 371 DVHSRVIFSDVAP----KEEHVRRGQVADVCLDTPLCNGHTTGMDVLWSGCPMVTLPAET 538
+ S+ + D+ P +H++ + D+ LDT G TT + L+ G P VT+
Sbjct: 710 GLESKRV--DLLPLILFNHDHMQAYSLMDISLDTFPYAGTTTTCESLYMGVPCVTMAGSV 767
Query: 539 LASRVAASQLNTLGCPELIAKSEEDYVNIAVRLGNEPKYLESVRNKVWKNRISSPLFNCQ 718
A V S L +G L+AK+E++YV ++V L ++ L +R + SP+ N
Sbjct: 768 HAHNVGVSLLTKVGLGHLVAKNEDEYVQLSVDLASDVTALSKLRMSLRDLMAGSPVCNGP 827
Query: 719 LYTRSLESLYQEMWNNFSNDRFDHITRL 802
+ LES Y+ MW + + R+
Sbjct: 828 SFAVGLESAYRNMWKKYCKGEVPSLRRM 855
>sp|Q8LP10|SPY_EUSGR Probable UDP-N-acetylglucosamine--peptide
N-acetylglucosaminyltransferase SPINDLY (EgSPY)
Length = 918
Score = 83.6 bits (205), Expect = 7e-16
Identities = 57/207 (27%), Positives = 99/207 (47%), Gaps = 3/207 (1%)
Frame = +2
Query: 191 LPDNATVFCNFNQLYKIGPDTLKMWVKILQNCPKSVLWLLRFPAAGE---KNVYKAFTDF 361
L + F +FN L KI P L++W +IL S L + P E +
Sbjct: 624 LSNGFITFGSFNNLAKITPKVLQVWARILCAVSNSRLIVKCKPFCCESVRQTFLSTLEQL 683
Query: 362 GLKDVHSRVIFSDVAPKEEHVRRGQVADVCLDTPLCNGHTTGMDVLWSGCPMVTLPAETL 541
GL+ ++ + +H++ + D+ LDT G TT + L+ G P +T+
Sbjct: 684 GLESTRVDLL-PLILLNHDHMQAYSLMDISLDTFPYAGTTTTCESLYMGVPCITMRGLVH 742
Query: 542 ASRVAASQLNTLGCPELIAKSEEDYVNIAVRLGNEPKYLESVRNKVWKNRISSPLFNCQL 721
A V S L+T+G L+AK+E+DYV +AV+L ++ L ++R + + SPL +
Sbjct: 743 AHNVGVSLLSTVGLGHLVAKNEDDYVRLAVQLASDVTALSNLRLTLRELMSKSPLCDGPK 802
Query: 722 YTRSLESLYQEMWNNFSNDRFDHITRL 802
+ + LE Y+ MW+ + ++R+
Sbjct: 803 FIQDLELTYRSMWHRYCKGDIPSLSRM 829
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 110,335,712
Number of Sequences: 369166
Number of extensions: 2502748
Number of successful extensions: 8338
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 7942
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8315
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8934348180
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)