Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_B18
(849 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q61545|EWS_MOUSE RNA-binding protein EWS 79 1e-14
sp|Q92804|RBP56_HUMAN TATA-binding protein associated facto... 79 1e-14
sp|Q01844|EWS_HUMAN RNA-binding protein EWS (EWS oncogene) ... 78 3e-14
sp|Q28009|FUS_BOVIN RNA-binding protein FUS (Pigpen protein) 74 5e-13
sp|P56959|FUS_MOUSE RNA-binding protein FUS (Pigpen protein) 73 9e-13
sp|P35637|FUS_HUMAN RNA-binding protein FUS (Oncogene FUS) ... 73 9e-13
sp|Q27294|CAZ_DROME RNA-binding protein cabeza (Sarcoma-ass... 69 1e-11
sp|O43120|UAP2_SCHPO Splicing factor U2AF-associated protein 2 54 5e-07
sp|P53830|YN26_YEAST Hypothetical 32.3 kDa protein in SEC21... 54 7e-07
sp|Q9P2K5|MYEF2_HUMAN Myelin expression factor 2 (MyEF-2) (... 47 7e-05
>sp|Q61545|EWS_MOUSE RNA-binding protein EWS
Length = 655
Score = 79.3 bits (194), Expect = 1e-14
Identities = 69/258 (26%), Positives = 99/258 (38%), Gaps = 34/258 (13%)
Frame = +2
Query: 155 GGSDAMNMAFQGMMSAQRGQLSNSERDNYNS-----------------NNDGALRLNTVY 283
GG D M+ +G RG L ER +N + D + +Y
Sbjct: 305 GGFDRGGMS-RGGRGGGRGGLGAGERGGFNKPGGPMDEGPDLDLGLPIDPDEDSDNSAIY 363
Query: 284 VSNLPQNLDHEMLKTQFSKAGSIKLNSKSGMPMIWIFKDR--GVPKGDALVTYDSNSSAS 457
V L N+ + L F + G +K+N ++G PMI I+ D+ G PKGDA V+Y+ +A
Sbjct: 364 VQGLNDNVTLDDLADFFKQCGVVKMNKRTGQPMIHIYLDKETGKPKGDATVSYEDPPTAK 423
Query: 458 NAVSYFKDHDFNGRRIEVRIA-----TNAERPILAPPSSXXXXXXXXXXXXXXXYKIESI 622
AV +F DF G +++V +A N+ R + P +
Sbjct: 424 AAVEWFDGKDFQGSKLKVSLARKKPPMNSMRGGMPpreGRGMPPPLRGGPGGPGGPGGPM 483
Query: 623 SRNNDYESDSYDWQKR--------EDGGFNKQREFXXXXXXXXXXXXGPDWICGK--CNN 772
R D + R GG N Q DW C C N
Sbjct: 484 GRMGGRGGDRGGFPPRGPRGSRGNPSGGGNVQHR-------------AGDWQCPNPGCGN 530
Query: 773 NNFSWRESCNRCQEPKAE 826
NF+WR CN+C+ PK E
Sbjct: 531 QNFAWRTECNQCKAPKPE 548
>sp|Q92804|RBP56_HUMAN TATA-binding protein associated factor 2N (RNA-binding protein 56)
(TAFII68) (TAF(II)68)
Length = 592
Score = 79.3 bits (194), Expect = 1e-14
Identities = 57/212 (26%), Positives = 91/212 (42%), Gaps = 4/212 (1%)
Frame = +2
Query: 209 GQLSNSERDNYNSNNDGALRLNTVYVSNLPQNLDHEMLKTQFSKAGSIKLNSKSGMPMIW 388
G ++++ ++ NS+N NT++V L + + + + F + G IK N K+G PMI
Sbjct: 219 GPRTDADSESDNSDN------NTIFVQGLGEGVSTDQVGEFFKQIGIIKTNKKTGKPMIN 272
Query: 389 IF--KDRGVPKGDALVTYDSNSSASNAVSYFKDHDFNGRRIEVRIATNAERPILAPPSSX 562
++ KD G PKG+A V++D SA A+ +F +F+G I+V AT
Sbjct: 273 LYTDKDTGKPKGEATVSFDDPPSAKAAIDWFDGKEFHGNIIKVSFATR------------ 320
Query: 563 XXXXXXXXXXXXXXYKIESISRNNDYESDSYDWQKREDGGFNKQREFXXXXXXXXXXXXG 742
R +R GG+ + F
Sbjct: 321 ---------------------RPEFMRGGGSGGGRRGRGGYRGRGGF----QGRGGDPKS 355
Query: 743 PDWIC--GKCNNNNFSWRESCNRCQEPKAENA 832
DW+C C N NF+ R SCN+C EP+ E++
Sbjct: 356 GDWVCPNPSCGNMNFARRNSCNQCNEPRPEDS 387
>sp|Q01844|EWS_HUMAN RNA-binding protein EWS (EWS oncogene) (Ewing sarcoma breakpoint
region 1 protein)
Length = 656
Score = 78.2 bits (191), Expect = 3e-14
Identities = 68/258 (26%), Positives = 98/258 (37%), Gaps = 34/258 (13%)
Frame = +2
Query: 155 GGSDAMNMAFQGMMSAQRGQLSNSERDNYNS-----------------NNDGALRLNTVY 283
GG D M+ G + G S ER +N + D + +Y
Sbjct: 305 GGFDRGGMSRGGRGGGRGGMGSAGERGGFNKPGGPMDEGPDLDLGPPVDPDEDSDNSAIY 364
Query: 284 VSNLPQNLDHEMLKTQFSKAGSIKLNSKSGMPMIWIFKDR--GVPKGDALVTYDSNSSAS 457
V L ++ + L F + G +K+N ++G PMI I+ D+ G PKGDA V+Y+ +A
Sbjct: 365 VQGLNDSVTLDDLADFFKQCGVVKMNKRTGQPMIHIYLDKETGKPKGDATVSYEDPPTAK 424
Query: 458 NAVSYFKDHDFNGRRIEVRIA-----TNAERPILAPPSSXXXXXXXXXXXXXXXYKIESI 622
AV +F DF G +++V +A N+ R L P +
Sbjct: 425 AAVEWFDGKDFQGSKLKVSLARKKPPMNSMRGGLPpreGRGMPPPLRGGPGGPGGPGGPM 484
Query: 623 SRNNDYESDSYDWQKR--------EDGGFNKQREFXXXXXXXXXXXXGPDWICGK--CNN 772
R D + R GG N Q DW C C N
Sbjct: 485 GRMGGRGGDRGGFPPRGPRGSRGNPSGGGNVQHR-------------AGDWQCPNPGCGN 531
Query: 773 NNFSWRESCNRCQEPKAE 826
NF+WR CN+C+ PK E
Sbjct: 532 QNFAWRTECNQCKAPKPE 549
>sp|Q28009|FUS_BOVIN RNA-binding protein FUS (Pigpen protein)
Length = 512
Score = 73.9 bits (180), Expect = 5e-13
Identities = 71/289 (24%), Positives = 107/289 (37%), Gaps = 24/289 (8%)
Frame = +2
Query: 32 PIIPSFGGVWDANQLQQMNSYGGMNPLLAQSLQGAQMQNMYGGSDAMNMAFQ-------- 187
P + S GG Q YGG Q +G + + GG + + ++
Sbjct: 180 PSMSSGGGGGGYGNQDQSGGYGG-----GQQDRGGRGRGGGGGYNRSSGGYEPRGRGGGR 234
Query: 188 ----GMMSAQRGQLSN--------SERDNYNSNNDGALRLNTVYVSNLPQNLDHEMLKTQ 331
GM + RG + S D+ N+D NT++V L +N+ E +
Sbjct: 235 GGRGGMGGSDRGGFNKFGGPRDQGSRHDSEQDNSDN----NTIFVQGLGENVTIESVADY 290
Query: 332 FSKAGSIKLNSKSGMPMIWIFKDR--GVPKGDALVTYDSNSSASNAVSYFKDHDFNGRRI 505
F + G IK N K+G PMI ++ DR G KG+A V++D SA A+ +F +F+G
Sbjct: 291 FKQIGIIKTNKKTGQPMINLYTDRETGKLKGEATVSFDDPPSAKAAIDWFDGKEFSGN-- 348
Query: 506 EVRIATNAERPILAPPSSXXXXXXXXXXXXXXXYKIESISRNNDYESDSYDWQKREDGGF 685
++++ R R Y GG
Sbjct: 349 PIKVSFATRRADFNRGGGNGRGGR---------------GRGGPMGRGGYGGGGSGGGG- 392
Query: 686 NKQREFXXXXXXXXXXXXGPDWICGK--CNNNNFSWRESCNRCQEPKAE 826
+ F DW C C N NFSWR CN+C+ PK +
Sbjct: 393 --RGGFPSGGGGGGGQQRAGDWKCPNPTCENMNFSWRNECNQCKAPKPD 439
>sp|P56959|FUS_MOUSE RNA-binding protein FUS (Pigpen protein)
Length = 518
Score = 73.2 bits (178), Expect = 9e-13
Identities = 61/228 (26%), Positives = 88/228 (38%), Gaps = 4/228 (1%)
Frame = +2
Query: 155 GGSDAMNMAFQGMMSAQRGQLSNSERDNYNSNNDGALRLNTVYVSNLPQNLDHEMLKTQF 334
GGSD R Q S + + NS+N NT++V L +N+ E + F
Sbjct: 248 GGSDRGGF---NKFGGPRDQGSRHDSEQDNSDN------NTIFVQGLGENVTIESVADYF 298
Query: 335 SKAGSIKLNSKSGMPMIWIFKDR--GVPKGDALVTYDSNSSASNAVSYFKDHDFNGRRIE 508
+ G IK N K+G PMI ++ DR G KG+A V++D SA A+ +F +F+G
Sbjct: 299 KQIGIIKTNKKTGQPMINLYTDRETGKLKGEATVSFDDPPSAKAAIDWFDGKEFSGN--P 356
Query: 509 VRIATNAERPILAPPSSXXXXXXXXXXXXXXXYKIESISRNNDYESDSYDWQKREDGGFN 688
++++ R R Y GG
Sbjct: 357 IKVSFATRRADFNRGGGNGRGGR---------------GRGGPMGRGGYGGGGSGGGG-- 399
Query: 689 KQREFXXXXXXXXXXXXGPDWICGK--CNNNNFSWRESCNRCQEPKAE 826
+ F DW C C N NFSWR CN+C+ PK +
Sbjct: 400 -RGGFPSGGGGGGGQQRAGDWKCPNPTCENMNFSWRNECNQCKAPKPD 446
>sp|P35637|FUS_HUMAN RNA-binding protein FUS (Oncogene FUS) (Oncogene TLS) (Translocated
in liposarcoma protein) (POMp75) (75 kDa DNA-pairing
protein)
Length = 526
Score = 73.2 bits (178), Expect = 9e-13
Identities = 61/228 (26%), Positives = 88/228 (38%), Gaps = 4/228 (1%)
Frame = +2
Query: 155 GGSDAMNMAFQGMMSAQRGQLSNSERDNYNSNNDGALRLNTVYVSNLPQNLDHEMLKTQF 334
GGSD R Q S + + NS+N NT++V L +N+ E + F
Sbjct: 255 GGSDRGGF---NKFGGPRDQGSRHDSEQDNSDN------NTIFVQGLGENVTIESVADYF 305
Query: 335 SKAGSIKLNSKSGMPMIWIFKDR--GVPKGDALVTYDSNSSASNAVSYFKDHDFNGRRIE 508
+ G IK N K+G PMI ++ DR G KG+A V++D SA A+ +F +F+G
Sbjct: 306 KQIGIIKTNKKTGQPMINLYTDRETGKLKGEATVSFDDPPSAKAAIDWFDGKEFSGN--P 363
Query: 509 VRIATNAERPILAPPSSXXXXXXXXXXXXXXXYKIESISRNNDYESDSYDWQKREDGGFN 688
++++ R R Y GG
Sbjct: 364 IKVSFATRRADFNRGGGNGRGGR---------------GRGGPMGRGGYGGGGSGGGG-- 406
Query: 689 KQREFXXXXXXXXXXXXGPDWICGK--CNNNNFSWRESCNRCQEPKAE 826
+ F DW C C N NFSWR CN+C+ PK +
Sbjct: 407 -RGGFPSGGGGGGGQQRAGDWKCPNPTCENMNFSWRNECNQCKAPKPD 453
>sp|Q27294|CAZ_DROME RNA-binding protein cabeza (Sarcoma-associated RNA-binding fly
homolog) (P19)
Length = 399
Score = 69.3 bits (168), Expect = 1e-11
Identities = 32/95 (33%), Positives = 57/95 (60%), Gaps = 2/95 (2%)
Frame = +2
Query: 242 NSNNDGALRLNTVYVSNLPQNLDHEMLKTQFSKAGSIKLNSKSGMPMIWIFKDR--GVPK 415
+ ND + +T++VS + + + ++T F G IK + ++ P IW++K++ G K
Sbjct: 110 SGGNDMITQEDTIFVSGMDPSTTEQDIETHFGAIGIIKKDKRTMKPKIWLYKNKETGASK 169
Query: 416 GDALVTYDSNSSASNAVSYFKDHDFNGRRIEVRIA 520
G+A VTYD ++A +A+ +F DFNG I+V +A
Sbjct: 170 GEATVTYDDTNAAQSAIEWFDGRDFNGNAIKVSLA 204
Score = 49.7 bits (117), Expect = 1e-05
Identities = 16/28 (57%), Positives = 20/28 (71%)
Frame = +2
Query: 746 DWICGKCNNNNFSWRESCNRCQEPKAEN 829
DW C CNN NF+WR CNRC+ PK ++
Sbjct: 278 DWKCNSCNNTNFAWRNECNRCKTPKGDD 305
>sp|O43120|UAP2_SCHPO Splicing factor U2AF-associated protein 2
Length = 367
Score = 53.9 bits (128), Expect = 5e-07
Identities = 49/169 (28%), Positives = 82/169 (48%), Gaps = 15/169 (8%)
Frame = +2
Query: 59 WDANQLQQMNSYGG----MNPLLAQSLQGAQMQNMYGGSDAM------NMAFQGMMSAQR 208
+D N L+ MN G ++ + A++ +G + N G D + + +G S R
Sbjct: 36 YDPNSLK-MNKAGSTGAEVSDVTAEATEGKESSN---GEDRHTKRLYESTSAEGYPSGSR 91
Query: 209 GQLSNSERDNYNSNNDGALRLN-TVYVSNLPQNLDHEMLKTQFSKAGSIKLNSKSGMPMI 385
+ S SE NS A +N VY+ LP ++ + ++ F K G I N +G P I
Sbjct: 92 NKKSKSE----NSEASPAPVINKAVYIQGLPLDVTVDEIEEVFKKCGVIAKNIDNGTPRI 147
Query: 386 WIFK-DRGVPKGDALVTYDSNSSASNAVSYFKDHDF---NGRRIEVRIA 520
I++ + G PKGDAL+ + + S A F D +F +G+++ V+ A
Sbjct: 148 KIYRTEDGTPKGDALIVFFRSESVELAEQLFDDTEFRYGSGQKMRVQKA 196
>sp|P53830|YN26_YEAST Hypothetical 32.3 kDa protein in SEC21-MRPL10 intergenic region
Length = 285
Score = 53.5 bits (127), Expect = 7e-07
Identities = 35/108 (32%), Positives = 54/108 (50%), Gaps = 2/108 (1%)
Frame = +2
Query: 203 QRGQLSNSERDNYNSNNDGALRLNTVYVSNLPQN-LDHEMLKTQFSKAGSIKLNSKSGMP 379
+R QL S + A + ++Y+S LP + E L QF K G I+ N + G P
Sbjct: 22 RRKQLKESNLQKRELEYNNASKNTSIYISGLPTDKTTKEGLTEQFCKYGMIRTN-RDGEP 80
Query: 380 MIWIF-KDRGVPKGDALVTYDSNSSASNAVSYFKDHDFNGRRIEVRIA 520
+ ++ D+G KGDAL+TY S + A+ + F G++I V A
Sbjct: 81 LCKLYVNDKGAFKGDALITYSKEESVTLAIEMMNESIFLGKQIRVERA 128
>sp|Q9P2K5|MYEF2_HUMAN Myelin expression factor 2 (MyEF-2) (MST156)
Length = 600
Score = 47.0 bits (110), Expect = 7e-05
Identities = 52/189 (27%), Positives = 77/189 (40%), Gaps = 17/189 (8%)
Frame = +2
Query: 14 FGANGNPIIPSFGGVWDANQLQQMNS--YGGMNPLLAQSLQGAQMQNMYGGSDAMNMAFQ 187
FG G+ +I F G ++ + + S GGM + S+ G M G D M+ +F
Sbjct: 432 FGRLGSAMIGGFAGRIGSSNMGPVGSGISGGMGSM--NSVTGG----MGMGLDRMSSSFD 485
Query: 188 GM-----------MSAQRGQLS----NSERDNYNSNNDGALRLNTVYVSNLPQNLDHEML 322
M + RG LS + R+ S N ++V NLP +L + L
Sbjct: 486 RMGPGIGAILERSIDMDRGFLSGPMGSGMRERIGSKG------NQIFVRNLPFDLTWQKL 539
Query: 323 KTQFSKAGSIKLNSKSGMPMIWIFKDRGVPKGDALVTYDSNSSASNAVSYFKDHDFNGRR 502
K +FS+ G + I + G KG V +DS SA A +GR
Sbjct: 540 KEKFSQCGHVMFAE--------IKMENGKSKGCGTVRFDSPESAEKACRIMNGIKISGRE 591
Query: 503 IEVRIATNA 529
I+VR+ NA
Sbjct: 592 IDVRLDRNA 600
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 92,601,957
Number of Sequences: 369166
Number of extensions: 1710963
Number of successful extensions: 4577
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4375
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4555
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8341863645
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)