Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_B12
(902 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q05052|OST48_CANFA Dolichyl-diphosphooligosaccharide--pr... 323 4e-88
sp|O54734|OST48_MOUSE Dolichyl-diphosphooligosaccharide--pr... 323 5e-88
sp|P39656|OST48_HUMAN Dolichyl-diphosphooligosaccharide--pr... 321 2e-87
sp|P48440|OST48_CHICK Dolichyl-diphosphooligosaccharide--pr... 320 3e-87
sp|Q24319|OST48_DROME Dolichyl-diphosphooligosaccharide--pr... 293 3e-79
sp|P45971|OST48_CAEEL Probable dolichyl-diphosphooligosacch... 267 3e-71
sp|P33767|OSTB_YEAST Dolichyl-diphosphooligosaccharide--pro... 93 1e-18
sp|O26270|PURQ_METTH Phosphoribosylformylglycinamidine synt... 35 0.38
sp|O78432|YCF30_GUITH Putative HTH-type transcriptional reg... 33 1.1
sp|O00159|MYO1C_HUMAN Myosin Ic (Myosin I beta) (MMI-beta) ... 32 2.4
>sp|Q05052|OST48_CANFA Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa
subunit precursor (Oligosaccharyl transferase 48 kDa
subunit)
Length = 445
Score = 323 bits (828), Expect = 4e-88
Identities = 163/281 (58%), Positives = 214/281 (76%), Gaps = 2/281 (0%)
Frame = +3
Query: 3 NVAKIPIIVGKVD-SPVLFKGVGIAADDQNPLLINLLHASSSAYSFNPDKPVDDYPNSVG 179
N+ K P IVG+ +P+LF+GVG+ AD NPL++++L SS++YSF PDKP+ YP++VG
Sbjct: 168 NLLKAPTIVGRSSLNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFPDKPITQYPHAVG 227
Query: 180 INTQLITALQARNNARVLFVGSLDLFSDDLFTASVEHAI-SKQTHARSGNEALATAISKW 356
NT LI LQARNNARV+F GSLD FSD F ++V+ A Q ++++GN LA A+S+W
Sbjct: 228 KNTLLIAGLQARNNARVVFSGSLDFFSDAFFNSAVQKAAPGSQRYSQTGNYELAVALSRW 287
Query: 357 VFKETGVLRVGNVSHSLADKSVENpreYTIMENVHYEIVIDELRADGTWTPFIADDVQLE 536
VFKE GVLRVG VSH ++ P YT+ + V Y IVI++L ++G W PF DD+QLE
Sbjct: 288 VFKEEGVLRVGPVSHHRVGETAP-PNAYTVTDLVEYSIVIEQL-SNGRWVPFDGDDIQLE 345
Query: 537 FYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFLVDYHRVGYTHVHSSTQVSVRPLMHT 716
F RIDPFVR + +K GK+ + FKLPDVYGVF+F VDY+R+GYTH+HSSTQVSVRPL HT
Sbjct: 346 FVRIDPFVRTFLKKKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLHSSTQVSVRPLQHT 405
Query: 717 QYERFIQAAYPYYASAISMMVGVILFSFLFLYFKDDKEKNE 839
QYERFI +AYPYYASA SMM+G+ +FS +FL+ K +KEK++
Sbjct: 406 QYERFIPSAYPYYASAFSMMLGLFIFSTVFLHMK-EKEKSD 445
>sp|O54734|OST48_MOUSE Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit) (DDOST 48 kDa subunit)
Length = 441
Score = 323 bits (827), Expect = 5e-88
Identities = 164/281 (58%), Positives = 212/281 (75%), Gaps = 2/281 (0%)
Frame = +3
Query: 3 NVAKIPIIVGKVD-SPVLFKGVGIAADDQNPLLINLLHASSSAYSFNPDKPVDDYPNSVG 179
N+ K P IVGK +P+LF+GVG+ AD NPL++++L SS++YSF PDKP+ YP++VG
Sbjct: 164 NLLKAPTIVGKSSLNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFPDKPITQYPHAVG 223
Query: 180 INTQLITALQARNNARVLFVGSLDLFSDDLFTASVEHAI-SKQTHARSGNEALATAISKW 356
NT LI LQARNNARV+F GSLD FSD F ++V+ A Q ++++GN LA A+S+W
Sbjct: 224 RNTLLIAGLQARNNARVIFSGSLDFFSDAFFNSAVQKATPGAQRYSQTGNYELAVALSRW 283
Query: 357 VFKETGVLRVGNVSHSLADKSVENpreYTIMENVHYEIVIDELRADGTWTPFIADDVQLE 536
VFKE GVLRVG VSH + P YT+ + V Y IVI++L ++G W PF DD+QLE
Sbjct: 284 VFKEEGVLRVGPVSHHRVGEMAP-PNAYTVTDLVEYSIVIEQL-SNGKWVPFDGDDIQLE 341
Query: 537 FYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFLVDYHRVGYTHVHSSTQVSVRPLMHT 716
F RIDPFVR + RK GK+ + FKLPDVYGVF+F VDY+R+GYTH++SSTQVSVRPL HT
Sbjct: 342 FVRIDPFVRTFLKRKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLYSSTQVSVRPLQHT 401
Query: 717 QYERFIQAAYPYYASAISMMVGVILFSFLFLYFKDDKEKNE 839
QYERFI +AYPYYASA SMM G+ +FS +FL+ K +KEK++
Sbjct: 402 QYERFIPSAYPYYASAFSMMAGLFIFSIVFLHMK-EKEKSD 441
>sp|P39656|OST48_HUMAN Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit) (DDOST 48 kDa subunit)
Length = 439
Score = 321 bits (823), Expect = 2e-87
Identities = 162/281 (57%), Positives = 212/281 (75%), Gaps = 2/281 (0%)
Frame = +3
Query: 3 NVAKIPIIVGKVD-SPVLFKGVGIAADDQNPLLINLLHASSSAYSFNPDKPVDDYPNSVG 179
N+ K P IVGK +P+LF+GVG+ AD NPL++++L SS++YSF PDKP+ YP++VG
Sbjct: 162 NLLKAPTIVGKSSLNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFPDKPITQYPHAVG 221
Query: 180 INTQLITALQARNNARVLFVGSLDLFSDDLFTASVEHAI-SKQTHARSGNEALATAISKW 356
NT LI LQARNNARV+F GSLD FSD F ++V+ A Q ++++GN LA A+S+W
Sbjct: 222 KNTLLIAGLQARNNARVIFSGSLDFFSDSFFNSAVQKAAPGSQRYSQTGNYELAVALSRW 281
Query: 357 VFKETGVLRVGNVSHSLADKSVENpreYTIMENVHYEIVIDELRADGTWTPFIADDVQLE 536
VFKE GVLRVG VSH ++ P YT+ + V Y IVI +L ++G W PF DD+QLE
Sbjct: 282 VFKEEGVLRVGPVSHHRVGETAP-PNAYTVTDLVEYSIVIQQL-SNGKWVPFDGDDIQLE 339
Query: 537 FYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFLVDYHRVGYTHVHSSTQVSVRPLMHT 716
F RIDPFVR + +K GK+ + FKLPDVYGVF+F VDY+R+GYTH++SSTQVSVRPL HT
Sbjct: 340 FVRIDPFVRTFLKKKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLYSSTQVSVRPLQHT 399
Query: 717 QYERFIQAAYPYYASAISMMVGVILFSFLFLYFKDDKEKNE 839
QYERFI +AYPYYASA MM+G+ +FS +FL+ K +KEK++
Sbjct: 400 QYERFIPSAYPYYASAFPMMLGLFIFSIVFLHMK-EKEKSD 439
>sp|P48440|OST48_CHICK Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit (Oligosaccharyl transferase 48 kDa subunit)
(OST 50 kDa subunit) (DDOST 48 kDa subunit)
Length = 413
Score = 320 bits (820), Expect = 3e-87
Identities = 163/281 (58%), Positives = 213/281 (75%), Gaps = 2/281 (0%)
Frame = +3
Query: 3 NVAKIPIIVGKVD-SPVLFKGVGIAADDQNPLLINLLHASSSAYSFNPDKPVDDYPNSVG 179
N+ K P IVGK +P+LF+GVG+ AD NPL++++L SS++YSF PDKP+ YP++VG
Sbjct: 136 NLLKAPTIVGKKALNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFPDKPITQYPHAVG 195
Query: 180 INTQLITALQARNNARVLFVGSLDLFSDDLFTASVEHAI-SKQTHARSGNEALATAISKW 356
NT LI LQARNNARV+F GSLD FSD F+++V+ A + ++++GN LA A+S+W
Sbjct: 196 KNTLLIAGLQARNNARVVFSGSLDFFSDAFFSSAVQKAAPGSKRYSQTGNYELAVALSRW 255
Query: 357 VFKETGVLRVGNVSHSLADKSVENpreYTIMENVHYEIVIDELRADGTWTPFIADDVQLE 536
VFKE GVLRVG VSH + + P YT+ + V Y IVI++L +DG W PF DD+QLE
Sbjct: 256 VFKEEGVLRVGAVSHHRVGE-LAPPNAYTVTDLVEYSIVIEKL-SDGKWIPFDGDDIQLE 313
Query: 537 FYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFLVDYHRVGYTHVHSSTQVSVRPLMHT 716
F RIDPFVR + R GK+ + FKLPDVYGVF+F VDY+R+GYTH++SSTQVSVRPL HT
Sbjct: 314 FVRIDPFVRTFLKRNGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLYSSTQVSVRPLQHT 373
Query: 717 QYERFIQAAYPYYASAISMMVGVILFSFLFLYFKDDKEKNE 839
QYERFI +AYPYYA A SMMVG+ +FS +FL+ K +KEK++
Sbjct: 374 QYERFIPSAYPYYAGAFSMMVGLFMFSIVFLHMK-EKEKSD 413
>sp|Q24319|OST48_DROME Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit) (DDOST 48 kDa subunit) (OST5OP) (DrOST)
(DmOST50)
Length = 449
Score = 293 bits (751), Expect = 3e-79
Identities = 152/280 (54%), Positives = 200/280 (71%), Gaps = 6/280 (2%)
Frame = +3
Query: 3 NVAKIPIIVGKVD-----SPVLFKGVGIAADDQNPLLINLLHASSSAYSFNPDKPVDDYP 167
N+ + IVGK + +P+L++G G+ AD +NPL++ LL A S+AYS+NP+ V DYP
Sbjct: 158 NLIQADTIVGKANRQADAAPLLYRGTGLIADKENPLVLKLLTAESTAYSYNPEASVSDYP 217
Query: 168 NSVGINTQLITALQARNNARVLFVGSLDLFSDDLFTASVEHAISKQTHARSGNEALATAI 347
++VG T LI ALQARNNARV+F GSL FSD+ FT +V++A S H +GN +A +I
Sbjct: 218 HAVGRGTLLIAALQARNNARVVFSGSLLFFSDESFTTAVQYAQSGVFHKLAGNRDVAESI 277
Query: 348 SKWVFKETGVLRVGNVSHSLADKSVENpreYTIMENVHYEIVIDELRADGTWTPFIADDV 527
SKWVF ETG LRV +V H + + + YTI + V Y I I+EL G W F A D+
Sbjct: 278 SKWVFGETGRLRVASVQHHKEGELLPPDQAYTITDPVVYTIGIEEL-VQGEWRAFKASDI 336
Query: 528 QLEFYRIDPFVRQNMLR-KEGKFYLDFKLPDVYGVFKFLVDYHRVGYTHVHSSTQVSVRP 704
QLEF RIDPFVR + + G + FK+PDVYGV++F VDY+RVGYTH++S+TQVSVRP
Sbjct: 337 QLEFVRIDPFVRTYLKQTNTGAYQAKFKIPDVYGVYQFKVDYNRVGYTHLYSTTQVSVRP 396
Query: 705 LMHTQYERFIQAAYPYYASAISMMVGVILFSFLFLYFKDD 824
L HTQYERFI +A+PYY SA SMM+GV +FSF+FL+FKD+
Sbjct: 397 LEHTQYERFIPSAFPYYTSAFSMMIGVFVFSFVFLHFKDE 436
>sp|P45971|OST48_CAEEL Probable dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 48 kDa subunit precursor
(Oligosaccharyl transferase 48 kDa subunit)
Length = 445
Score = 267 bits (683), Expect = 3e-71
Identities = 137/263 (52%), Positives = 177/263 (67%)
Frame = +3
Query: 45 PVLFKGVGIAADDQNPLLINLLHASSSAYSFNPDKPVDDYPNSVGINTQLITALQARNNA 224
PVLFKG+G+ A N L ++++ AS +AYS++P P+ G T L+ LQ+RNNA
Sbjct: 172 PVLFKGIGLVAGKTNNLALSIVRASGTAYSYDPKAVRATNPSIAGSRTLLVGGLQSRNNA 231
Query: 225 RVLFVGSLDLFSDDLFTASVEHAISKQTHARSGNEALATAISKWVFKETGVLRVGNVSHS 404
R++F GS +LFS+ F+A A+SGN ATAI++WV KE+GVLRV V+H
Sbjct: 232 RIVFTGSSELFSNTFFSAKTNSVNPSVQGAQSGNADFATAITRWVMKESGVLRVKTVNHH 291
Query: 405 LADKSVENpreYTIMENVHYEIVIDELRADGTWTPFIADDVQLEFYRIDPFVRQNMLRKE 584
++V Y I E+V Y I I+EL+ +G W PF DVQLEF RIDPFVR +
Sbjct: 292 KKGETVPPVEGYFITEDVVYTIEIEELK-NGKWVPFQGKDVQLEFVRIDPFVRATLKNSN 350
Query: 585 GKFYLDFKLPDVYGVFKFLVDYHRVGYTHVHSSTQVSVRPLMHTQYERFIQAAYPYYASA 764
G+ + FKLPDV GVFKFLVDY RVGYTH++ QVSVRPL HTQYERFI++AYPYYAS+
Sbjct: 351 GRLSVAFKLPDVLGVFKFLVDYRRVGYTHLYDVQQVSVRPLWHTQYERFIRSAYPYYASS 410
Query: 765 ISMMVGVILFSFLFLYFKDDKEK 833
SMM G++LFS ++LY KD K
Sbjct: 411 FSMMAGLVLFSIVYLYHKDTPVK 433
>sp|P33767|OSTB_YEAST Dolichyl-diphosphooligosaccharide--protein glycosyltransferase beta
subunit precursor (Oligosaccharyl transferase beta
subunit)
Length = 430
Score = 92.8 bits (229), Expect = 1e-18
Identities = 60/214 (28%), Positives = 100/214 (46%), Gaps = 8/214 (3%)
Frame = +3
Query: 192 LITALQARNNARVLFVGSLDLFSDDLFTASVEHAISKQTHARSGNEALATAISKWVFKET 371
L+ Q NNAR++++GS D + + N+ A + KW F E
Sbjct: 217 LVVGFQNLNNARLVWIGSSDFLKNK---------------NQDSNQEFAKELLKWTFNEK 261
Query: 372 GVLRVGNVSHSLADKSVENpreYTIMENVHYEIVIDELRADGTWTPFIADDVQLEFYRID 551
V++ + HS AD + + Y I + V Y + E + W P IADD+Q E ++D
Sbjct: 262 SVIKSVHAVHSHADGTSYDEEPYKIKDKVIYSVGFSEWNGE-EWLPHIADDIQFELRQVD 320
Query: 552 PFVRQNMLRK----EGKFYL--DFKLPDVYGVFKFLVDYHRVGYTHVHSSTQVSVRPLMH 713
P+ R + E ++Y +F LPD +GVF FL DY ++G + ++R L +
Sbjct: 321 PYYRLTLSPSGNDSETQYYTTGEFILPDRHGVFTFLTDYRKIGLSFTTDKDVKAIRHLAN 380
Query: 714 TQYERF--IQAAYPYYASAISMMVGVILFSFLFL 809
+Y R I ++ Y ++ ++V I F F+
Sbjct: 381 DEYPRSWEISNSWVYISAICGVIVAWIFFVVSFV 414
>sp|O26270|PURQ_METTH Phosphoribosylformylglycinamidine synthase I (FGAM synthase I)
Length = 218
Score = 34.7 bits (78), Expect = 0.38
Identities = 33/135 (24%), Positives = 60/135 (44%), Gaps = 4/135 (2%)
Frame = +3
Query: 261 DDLFTASVEHAISKQTHARSGNEALAT----AISKWVFKETGVLRVGNVSHSLADKSVEN 428
D L + S + R+G A T A+ + V +E VL + N + LA+ +
Sbjct: 39 DHLDAVVIPGGFSYGDYLRAGAIAAITPVMDAVRELVREEKPVLGICNGAQILAEVGLV- 97
Query: 429 preYTIMENVHYEIVIDELRADGTWTPFIADDVQLEFYRIDPFVRQNMLRKEGKFYLDFK 608
P +T+ E+ + ELR T TPF ++ D +R + EG++Y D
Sbjct: 98 PGVFTVNEHPKFNCQWTELRVKTTRTPFTG------LFKKDEVIRMPVAHAEGRYYHD-N 150
Query: 609 LPDVYGVFKFLVDYH 653
+ +V+ + ++ +H
Sbjct: 151 ISEVWENDQVVLQFH 165
>sp|O78432|YCF30_GUITH Putative HTH-type transcriptional regulator ycf30
Length = 310
Score = 33.1 bits (74), Expect = 1.1
Identities = 17/59 (28%), Positives = 32/59 (54%)
Frame = +3
Query: 471 VIDELRADGTWTPFIADDVQLEFYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFLVD 647
V DELR+ TP++ D++ L ++ PF + +++KE + L F D + ++D
Sbjct: 155 VPDELRSKLQITPYVDDELALILPKLHPFSKLEVIQKEDLYRLRFITLDKQSTIRRVID 213
>sp|O00159|MYO1C_HUMAN Myosin Ic (Myosin I beta) (MMI-beta) (MMIb)
Length = 1028
Score = 32.0 bits (71), Expect = 2.4
Identities = 36/158 (22%), Positives = 64/158 (40%), Gaps = 17/158 (10%)
Frame = +3
Query: 9 AKIPIIVGKVDSPVLFKGVGIA------ADDQNPLLINLLHASSSAYSFNPDKPVDDYPN 170
AK+ I K D V+ K + + +D ++ ++LH + ++ N D
Sbjct: 238 AKVSSINDKSDWKVVRKALTVIDFTEDEVEDLLSIVASVLHLGNIHFAANEDS------- 290
Query: 171 SVGINTQLITALQARNNARVLFVGSLDLFSDDLFTASVEHAISKQTHARSGNEALA---- 338
N Q+ T Q + R+L V +++ A++ + G E L+
Sbjct: 291 ----NAQVTTENQLKYLTRLLSVEG----------STLREALTHRKIIAKGEELLSPLNL 336
Query: 339 -------TAISKWVFKETGVLRVGNVSHSLADKSVENP 431
A++K V+ T VG ++ SLA K VE+P
Sbjct: 337 EQAAYARNALAKAVYSRTFTWLVGKINRSLASKDVESP 374
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 99,388,452
Number of Sequences: 369166
Number of extensions: 2034264
Number of successful extensions: 5764
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5528
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5742
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9126484700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)