Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_B07
(532 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P81305|Y22B_METJA Hypothetical protein MJ0226.2 33 0.43
sp|P29867|NU2M_DROMA NADH-ubiquinone oxidoreductase chain 2... 32 1.3
sp|P41820|BFR1_SCHPO Brefeldin A resistance protein 31 2.1
sp|Q9HBG4|VPP4_HUMAN Vacuolar proton translocating ATPase 1... 31 2.1
sp|P21421|RPOB_PLAFA DNA-directed RNA polymerase beta chain 30 2.8
sp|P03896|NU2M_DROME NADH-ubiquinone oxidoreductase chain 2... 30 2.8
sp|P24880|NU4M_ASCSU NADH-ubiquinone oxidoreductase chain 4... 30 3.6
sp|Q920R6|VPP4_MOUSE Vacuolar proton translocating ATPase 1... 30 3.6
sp|P29869|NU2M_DROSI NADH-ubiquinone oxidoreductase chain 2... 30 3.6
sp|P48906|NU2M_PICCA NADH-ubiquinone oxidoreductase chain 2... 30 4.8
>sp|P81305|Y22B_METJA Hypothetical protein MJ0226.2
Length = 122
Score = 33.1 bits (74), Expect = 0.43
Identities = 27/83 (32%), Positives = 41/83 (49%), Gaps = 11/83 (13%)
Frame = -2
Query: 333 LWLIFNFIISLYSFISIHIFKLHSAQLI-------SQFDPY*FNTILLFIYLISIDFMNF 175
L IF FI SLY+FI + + ++I F PY F +ILL ++ + F F
Sbjct: 18 LSFIFGFI-SLYAFIKYSKLERKNKEIIYVYVDNLKNFSPYMFYSILLTFTIVFVIFALF 76
Query: 174 I----YYDFHIHINMLFVWE*II 118
I +D ++ + MLF + II
Sbjct: 77 ICFVFNFDLYLGLTMLFSYFLII 99
>sp|P29867|NU2M_DROMA NADH-ubiquinone oxidoreductase chain 2 (NADH dehydrogenase subunit
2)
Length = 274
Score = 31.6 bits (70), Expect = 1.3
Identities = 19/68 (27%), Positives = 33/68 (48%)
Frame = -2
Query: 375 HISFTK*LLIIKNDLWLIFNFIISLYSFISIHIFKLHSAQLISQFDPY*FNTILLFIYLI 196
H+ + L+I +WLI+ F S SF+ +F + ++Q + N+ +L L
Sbjct: 115 HLGWMLSSLMISESIWLIYFFFYSFLSFVLTFMFNIFKLFHLNQLFSWFVNSKILKFTL- 173
Query: 195 SIDFMNFI 172
FMNF+
Sbjct: 174 ---FMNFL 178
>sp|P41820|BFR1_SCHPO Brefeldin A resistance protein
Length = 1530
Score = 30.8 bits (68), Expect = 2.1
Identities = 21/57 (36%), Positives = 28/57 (49%), Gaps = 6/57 (10%)
Frame = -3
Query: 500 FITTQGFGFKFQKFYINSNFYS-KLSETHVYASGKIL*FYISF-----TSHLQNNFS 348
+I + F F FQ I S FY KL+ V++ G +L F I F S + N FS
Sbjct: 540 YIGSMAFAFLFQSLIIGSIFYDMKLNTVDVFSRGGVLFFSILFCALQSLSEIANMFS 596
>sp|Q9HBG4|VPP4_HUMAN Vacuolar proton translocating ATPase 116 kDa subunit a isoform 4
(V-ATPase 116-kDa isoform a4) (Vacuolar proton
translocating ATPase 116 kDa subunit a kidney isoform)
Length = 840
Score = 30.8 bits (68), Expect = 2.1
Identities = 18/72 (25%), Positives = 36/72 (50%)
Frame = -2
Query: 369 SFTK*LLIIKNDLWLIFNFIISLYSFISIHIFKLHSAQLISQFDPY*FNTILLFIYLISI 190
S+ + +I + ++F I+SL++ HI+ + +I QF P + LF YL+ +
Sbjct: 538 SYKMKMSVILGIVQMVFGVILSLFN----HIYFRRTLNIILQFIPEMIFILCLFGYLVFM 593
Query: 189 DFMNFIYYDFHI 154
+ +D H+
Sbjct: 594 IIFKWCCFDVHV 605
>sp|P21421|RPOB_PLAFA DNA-directed RNA polymerase beta chain
Length = 1024
Score = 30.4 bits (67), Expect = 2.8
Identities = 30/110 (27%), Positives = 55/110 (50%), Gaps = 9/110 (8%)
Frame = -2
Query: 405 WQNIMILYFIHISFTK*LLIIKNDLWLIFNF------IISLYSFISIH---IFKLHSAQL 253
+ NI+IL ++ + + L KN+ I F II +Y +IS+ IFK+ +
Sbjct: 105 YNNIIILNGLYKTCIQ--LFKKNNKIFIIKFKNNNKNIIYVYIYISLGLRIIFKISKLNI 162
Query: 252 ISQFDPY*FNTILLFIYLISIDFMNFIYYDFHIHINMLFVWE*IIVYNGI 103
F+ + FN ++L +YL +I +I + + I + + I++YN I
Sbjct: 163 DCYFNNFKFNFLILLLYLNNI----YINKNISLFIYNNIINKKILIYNYI 208
>sp|P03896|NU2M_DROME NADH-ubiquinone oxidoreductase chain 2 (NADH dehydrogenase subunit
2)
Length = 341
Score = 30.4 bits (67), Expect = 2.8
Identities = 19/68 (27%), Positives = 32/68 (47%)
Frame = -2
Query: 375 HISFTK*LLIIKNDLWLIFNFIISLYSFISIHIFKLHSAQLISQFDPY*FNTILLFIYLI 196
H+ + L+I +WLI F S SF+ +F + ++Q + N+ +L L
Sbjct: 182 HLGWMLSSLMISESIWLILFFFYSFLSFVLTFMFNIFKLFHLNQLFSWFVNSKILKFTL- 240
Query: 195 SIDFMNFI 172
FMNF+
Sbjct: 241 ---FMNFL 245
>sp|P24880|NU4M_ASCSU NADH-ubiquinone oxidoreductase chain 4 (NADH dehydrogenase subunit
4)
Length = 409
Score = 30.0 bits (66), Expect = 3.6
Identities = 11/27 (40%), Positives = 19/27 (70%)
Frame = -2
Query: 213 LFIYLISIDFMNFIYYDFHIHINMLFV 133
LF+Y S F++ +Y+DF++ M+FV
Sbjct: 136 LFVYFKSFFFISLVYFDFNLSWEMVFV 162
>sp|Q920R6|VPP4_MOUSE Vacuolar proton translocating ATPase 116 kDa subunit a isoform 4
(V-ATPase 116-kDa isoform a4) (Vacuolar proton
translocating ATPase 116 kDa subunit a kidney isoform)
Length = 833
Score = 30.0 bits (66), Expect = 3.6
Identities = 18/57 (31%), Positives = 29/57 (50%)
Frame = -2
Query: 327 LIFNFIISLYSFISIHIFKLHSAQLISQFDPY*FNTILLFIYLISIDFMNFIYYDFH 157
+IF I+SL++ HI+ + +I QF P + LF YL+ + + YD H
Sbjct: 552 MIFGVILSLFN----HIYFRRTLNIILQFIPEMIFMLSLFGYLVFMIIFKWCRYDAH 604
>sp|P29869|NU2M_DROSI NADH-ubiquinone oxidoreductase chain 2 (NADH dehydrogenase subunit
2)
sp|P29868|NU2M_DROSE NADH-ubiquinone oxidoreductase chain 2 (NADH dehydrogenase subunit
2)
Length = 274
Score = 30.0 bits (66), Expect = 3.6
Identities = 18/68 (26%), Positives = 32/68 (47%)
Frame = -2
Query: 375 HISFTK*LLIIKNDLWLIFNFIISLYSFISIHIFKLHSAQLISQFDPY*FNTILLFIYLI 196
H+ + L+ +WLI+ F S SF+ +F + ++Q + N+ +L L
Sbjct: 115 HLGWMLSSLMFSESIWLIYFFFYSFLSFVLTFMFNIFKLFHLNQLFSWFVNSKILKFTL- 173
Query: 195 SIDFMNFI 172
FMNF+
Sbjct: 174 ---FMNFL 178
>sp|P48906|NU2M_PICCA NADH-ubiquinone oxidoreductase chain 2 (NADH dehydrogenase subunit
2)
Length = 567
Score = 29.6 bits (65), Expect = 4.8
Identities = 24/71 (33%), Positives = 38/71 (53%), Gaps = 3/71 (4%)
Frame = -2
Query: 345 IKNDLWLIFNFIISLYSFISI-HIFKLHSAQ--LISQFDPY*FNTILLFIYLISIDFMNF 175
IKN+ +LIF II + SFI I +F + LIS + N + L + LI ++
Sbjct: 417 IKNNRYLIFCLIIIIGSFIGIPPLFGFYGKYYLLISSINS---NYLFLSLLLIISSIISS 473
Query: 174 IYYDFHIHINM 142
IYY + ++I +
Sbjct: 474 IYYLYFLNITL 484
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 45,087,299
Number of Sequences: 369166
Number of extensions: 706996
Number of successful extensions: 1302
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1275
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1302
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3600891075
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)