Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_010_P17
(831 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q5R5J4|VPS53_PONPY Vacuolar protein sorting protein 53 194 3e-49
sp|Q5VIR6|VPS53_HUMAN Vacuolar protein sorting protein 53 193 6e-49
sp|P34561|YNP8_CAEEL Hypothetical protein T05G5.8 in chromo... 114 3e-25
sp|P87129|YDLF_SCHPO Hypothetical protein C3A12.15 in chrom... 75 3e-13
sp|Q8K0U4|HS12A_MOUSE Heat shock 70 kDa protein 12A 33 0.74
sp|Q9SL29|CNG15_ARATH Putative cyclic nucleotide-gated ion ... 33 0.96
sp|O06994|O16G_BACSU Oligo-1,6-glucosidase (Oligosaccharide... 33 1.3
sp|P34160|GCR3_YEAST Protein GCR3 (Protein STO1) (Protein S... 32 1.6
sp|P13395|SPTCA_DROME Spectrin alpha chain 32 2.8
sp|Q7VAW6|OTC_PROMA Ornithine carbamoyltransferase (OTCase) 32 2.8
>sp|Q5R5J4|VPS53_PONPY Vacuolar protein sorting protein 53
Length = 699
Score = 194 bits (492), Expect = 3e-49
Identities = 105/243 (43%), Positives = 154/243 (63%), Gaps = 3/243 (1%)
Frame = +3
Query: 111 LNSATNLFLLYRKLLNQAPQLSRGQALVDLSKVLGKYLREYNNKVLLAQLPLKKTGVLSM 290
L S +LF+ Y+K + Q QLS G+ ++ L+ + KYLREY K+L LP
Sbjct: 468 LPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLP--------- 518
Query: 291 ANSLTGSGGTVLSANQIIQSLLKDEQ---IFRLSKEDIKKVSVILVTACYCLKTVEELEK 461
+ T SGG +S SLLK+++ + + + E++ + IL TA YCL T ++LE+
Sbjct: 519 -KTTTSSGGLTIS------SLLKEKEGSEVAKFTLEELCLICSILSTAEYCLATTQQLEE 571
Query: 462 RLKSKVKPPALSKDIDFSGEIELFNQCRTSCVQLMSSDLEGACDPGLTAMHKMSWASIEV 641
+LK KV + + I+ +GE++ F+ +S +QL+ DL+ ACDP LTAM KM W ++E
Sbjct: 572 KLKEKVDVSLIER-INLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMSKMQWQNVEH 630
Query: 642 VGDQSNYVTSIISHLRENIPLIRQYLHNVRAFFTQFCIQFVNGFISKFISALFKCRVTST 821
VGDQS YVTS+I H+++N+P+IR L + R +FTQFCI+F N FI KFI+ LFKC+ S
Sbjct: 631 VGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCIKFANSFIPKFITHLFKCKPISM 690
Query: 822 VGA 830
VGA
Sbjct: 691 VGA 693
>sp|Q5VIR6|VPS53_HUMAN Vacuolar protein sorting protein 53
Length = 699
Score = 193 bits (490), Expect = 6e-49
Identities = 104/243 (42%), Positives = 154/243 (63%), Gaps = 3/243 (1%)
Frame = +3
Query: 111 LNSATNLFLLYRKLLNQAPQLSRGQALVDLSKVLGKYLREYNNKVLLAQLPLKKTGVLSM 290
L S +LF+ Y+K + Q QLS G+ ++ L+ + KYLREY K+L LP
Sbjct: 468 LPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLP--------- 518
Query: 291 ANSLTGSGGTVLSANQIIQSLLKDEQ---IFRLSKEDIKKVSVILVTACYCLKTVEELEK 461
+ T SGG +S SLLK+++ + + + E++ + IL TA YCL T ++LE+
Sbjct: 519 -KTTTSSGGLTIS------SLLKEKEGSEVAKFTLEELCLICNILSTAEYCLATTQQLEE 571
Query: 462 RLKSKVKPPALSKDIDFSGEIELFNQCRTSCVQLMSSDLEGACDPGLTAMHKMSWASIEV 641
+LK KV + + I+ +GE++ F+ +S +QL+ DL+ ACDP LTAM KM W ++E
Sbjct: 572 KLKEKVDVSLIER-INLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMSKMQWQNVEH 630
Query: 642 VGDQSNYVTSIISHLRENIPLIRQYLHNVRAFFTQFCIQFVNGFISKFISALFKCRVTST 821
VGDQS YVTS+I H+++N+P+IR L + R +FTQFC++F N FI KFI+ LFKC+ S
Sbjct: 631 VGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCVKFANSFIPKFITHLFKCKPISM 690
Query: 822 VGA 830
VGA
Sbjct: 691 VGA 693
>sp|P34561|YNP8_CAEEL Hypothetical protein T05G5.8 in chromosome III
Length = 808
Score = 114 bits (285), Expect = 3e-25
Identities = 81/286 (28%), Positives = 143/286 (50%), Gaps = 18/286 (6%)
Frame = +3
Query: 27 KDKEFEEILGESRDPITDNYSDDSAENSLN-----SATNLFLLYRKLLNQAPQLS-RGQA 188
++K E L I S E+S + S+ ++FLL +K++ ++ +LS A
Sbjct: 374 QEKTLNEFLDTCASKIRSGEEKPSRESSTHAVPFPSSADMFLLLKKVITESSKLSSEPDA 433
Query: 189 LV-DLSKVLGKYLREYNNKVLLAQLPLKKTGVLSMANSLTGSGGTVLSANQIIQSLLKDE 365
L+ D+ V+ LR Y L+A LP S+ + +G+ + SL+++E
Sbjct: 434 LIRDVIGVVRVCLRGYATSCLVAFLP-------SLGSQQSGAAN--------LFSLIREE 478
Query: 366 QIF-RLSKEDIKKVSVILVTACYCLKTVEELEKRLKSKVKPPALSKDIDFSGEIELFNQC 542
+ RL+ + V IL TA +C +T +L+++L ++ +D S E E F
Sbjct: 479 IAYPRLTPDQQFLVCCILATADWCAETSIQLQEKLSQRIP------GVDISQETEAFYSI 532
Query: 543 RTSCVQLMSSDLEGACDPGLTAMHKM----------SWASIEVVGDQSNYVTSIISHLRE 692
+Q++ D+E CD L ++ K+ +W +++ VGD+S ++ S+ +HLR+
Sbjct: 533 TNQSLQVLVQDVESTCDAALQSISKVKCSIFVRIEKTWTAVDCVGDESPFIGSMRAHLRQ 592
Query: 693 NIPLIRQYLHNVRAFFTQFCIQFVNGFISKFISALFKCRVTSTVGA 830
+PLIR L + R +F FC++ KF+ +LF+CR ST GA
Sbjct: 593 AVPLIRDMLSDRRKYFAHFCLKLATQLAHKFVGSLFRCRTISTHGA 638
>sp|P87129|YDLF_SCHPO Hypothetical protein C3A12.15 in chromosome I
Length = 756
Score = 74.7 bits (182), Expect = 3e-13
Identities = 62/248 (25%), Positives = 101/248 (40%)
Frame = +3
Query: 87 SDDSAENSLNSATNLFLLYRKLLNQAPQLSRGQALVDLSKVLGKYLREYNNKVLLAQLPL 266
S D + L+S+ LF YRK L Q +L+R LV L + K+LR Y LL
Sbjct: 395 STDESSQVLSSSIKLFQAYRKTLTQFVRLTRSSPLVGLKNLFIKWLRRYTQVELL----- 449
Query: 267 KKTGVLSMANSLTGSGGTVLSANQIIQSLLKDEQIFRLSKEDIKKVSVILVTACYCLKTV 446
D Q K +++ L TA Y +T
Sbjct: 450 -------------------------------DYQ----ESSTFKDIAIRLNTAEYIYRTT 474
Query: 447 EELEKRLKSKVKPPALSKDIDFSGEIELFNQCRTSCVQLMSSDLEGACDPGLTAMHKMSW 626
ELEKR + ++ + FS +E+ + R + ++ + E + L + KM
Sbjct: 475 IELEKRFQ-EISNKEFKDKMSFSEVLEVISSSRGTLLKFATGKFENVLNSDLEPLSKMDL 533
Query: 627 ASIEVVGDQSNYVTSIISHLRENIPLIRQYLHNVRAFFTQFCIQFVNGFISKFISALFKC 806
+IE VGDQS+YV + ++ + ++ F FC + F +F++A++
Sbjct: 534 KNIETVGDQSSYVGGAVQNMTAKASEFLSVV-DLNMFARNFCDRSCESFTRQFLNAIYLA 592
Query: 807 RVTSTVGA 830
+ S VGA
Sbjct: 593 KPISEVGA 600
>sp|Q8K0U4|HS12A_MOUSE Heat shock 70 kDa protein 12A
Length = 675
Score = 33.5 bits (75), Expect = 0.74
Identities = 22/73 (30%), Positives = 37/73 (50%)
Frame = +3
Query: 207 VLGKYLREYNNKVLLAQLPLKKTGVLSMANSLTGSGGTVLSANQIIQSLLKDEQIFRLSK 386
++ Y E++N + +KK G L + LTGSGGT + A + IQ+ I +
Sbjct: 599 IINIYSSEHDNVSFITDPGVKKCGTLRL--DLTGSGGTAVPARREIQT------IMQFGD 650
Query: 387 EDIKKVSVILVTA 425
+IK +V + T+
Sbjct: 651 TEIKATAVDITTS 663
>sp|Q9SL29|CNG15_ARATH Putative cyclic nucleotide-gated ion channel 15 (Cyclic
nucleotide-and calmodulin-regulated ion channel 15)
Length = 678
Score = 33.1 bits (74), Expect = 0.96
Identities = 28/87 (32%), Positives = 44/87 (50%), Gaps = 4/87 (4%)
Frame = +3
Query: 177 RGQALVDLSKVLGKYLREYNNKVLLAQLPLKKTGVLSMANSLTGSGGT----VLSANQII 344
RG+ ++D K+ +YL + L+A LPL + + + +L GS T VL I
Sbjct: 152 RGELVIDSRKIAWRYLHKSFWIHLVAALPLPQVLIWIIIPNLRGSPMTNTKNVLRFIIIF 211
Query: 345 QSLLKDEQIFRLSKEDIKKVSVILVTA 425
Q + + IF LS++ IK V+ TA
Sbjct: 212 QYVPRMFLIFPLSRQIIKATGVVTETA 238
>sp|O06994|O16G_BACSU Oligo-1,6-glucosidase (Oligosaccharide alpha-1,6-glucosidase)
(Sucrase-isomaltase) (Isomaltase) (Dextrin
6-alpha-D-glucanohydrolase)
Length = 561
Score = 32.7 bits (73), Expect = 1.3
Identities = 22/82 (26%), Positives = 35/82 (42%), Gaps = 5/82 (6%)
Frame = +3
Query: 54 GESRDPITDNYSDDSAENSLNSATNLFLLYRKLLNQAPQ-----LSRGQALVDLSKVLGK 218
G+ P+ Y D + + SL ++F Y+KL+ Q Q L + +
Sbjct: 438 GDPWIPVNSRYQDINVKESLEDQDSIFFYYQKLIQLRKQYKIMIYGDYQLLQENDPQVFS 497
Query: 219 YLREYNNKVLLAQLPLKKTGVL 284
YLREY + LL + L + L
Sbjct: 498 YLREYRGEKLLVVVNLSEEKAL 519
>sp|P34160|GCR3_YEAST Protein GCR3 (Protein STO1) (Protein SUT1)
Length = 861
Score = 32.3 bits (72), Expect = 1.6
Identities = 24/96 (25%), Positives = 48/96 (50%), Gaps = 2/96 (2%)
Frame = +3
Query: 144 RKLLNQAPQLSRGQALVDLSKVLGKYLREYNNKVLLAQLPLKKTGVLSMANSLTGSGGTV 323
RK+L+ + + + + +L +LG+ EY + + + ++ + ++T SG
Sbjct: 595 RKILDYTHKANNSREVTELESILGELKNEYGSII----SDFNRFVIILLVQAVTDSGSRS 650
Query: 324 LS-ANQIIQSLLKD-EQIFRLSKEDIKKVSVILVTA 425
LS AN+ I L +D + IF + DI+ I++ A
Sbjct: 651 LSHANKYINDLKEDLKTIFAKIELDIETKEYIIIEA 686
>sp|P13395|SPTCA_DROME Spectrin alpha chain
Length = 2415
Score = 31.6 bits (70), Expect = 2.8
Identities = 20/77 (25%), Positives = 38/77 (49%), Gaps = 1/77 (1%)
Frame = +3
Query: 33 KEFEEILGESRDPITDNYSDDSAENSLNSA-TNLFLLYRKLLNQAPQLSRGQALVDLSKV 209
K+ + L S+ + DN+S +N +NS NL L R+ N+ + + LV +
Sbjct: 1025 KKIDAGLSASQQNLVDNHSIAKRQNQINSQYDNLLALARERQNKLNETVKAYVLVREAAD 1084
Query: 210 LGKYLREYNNKVLLAQL 260
L +++R+ N +A +
Sbjct: 1085 LAQWIRDKENHAQIADV 1101
>sp|Q7VAW6|OTC_PROMA Ornithine carbamoyltransferase (OTCase)
Length = 318
Score = 31.6 bits (70), Expect = 2.8
Identities = 21/57 (36%), Positives = 31/57 (54%), Gaps = 5/57 (8%)
Frame = +3
Query: 150 LLNQAPQLSRGQALVDLSKVLGKY-----LREYNNKVLLAQLPLKKTGVLSMANSLT 305
L NQA QL+RG+ L D ++VL +Y LR + N L + K + + N+LT
Sbjct: 84 LSNQATQLARGEPLKDTARVLSRYCDALALRTFGNDEL---IEYAKWSSIPVINALT 137
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 83,806,946
Number of Sequences: 369166
Number of extensions: 1575975
Number of successful extensions: 4324
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4199
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4318
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8052550455
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)