Planarian EST Database


Dr_sW_010_P14-2

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_010_P14-2
         (392 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O43852|CALU_HUMAN  Calumenin precursor (Crocalbin) (IEF S...    97   2e-20
sp|O35887|CALU_MOUSE  Calumenin precursor (Crocalbin)              97   2e-20
sp|O35783|CALU_RAT  Calumenin precursor (Crocalbin) (CBP-50)       95   6e-20
sp|Q15293|RCN1_HUMAN  Reticulocalbin-1 precursor                   88   7e-18
sp|Q05186|RCN1_MOUSE  Reticulocalbin-1 precursor                   87   1e-17
sp|O93434|RCN1_FUGRU  Reticulocalbin-1 precursor                   84   1e-16
sp|Q8BH97|RCN3_MOUSE  Reticulocalbin-3 precursor                   82   3e-16
sp|Q96D15|RCN3_HUMAN  Reticulocalbin-3 precursor (EF-hand ca...    80   1e-15
sp|Q62703|RCN2_RAT  Reticulocalbin-2 precursor (Calcium-bind...    57   2e-08
sp|Q8BP92|RCN2_MOUSE  Reticulocalbin-2 precursor (Taipoxin-a...    57   2e-08
>sp|O43852|CALU_HUMAN Calumenin precursor (Crocalbin) (IEF SSP 9302)
          Length = 315

 Score = 96.7 bits (239), Expect = 2e-20
 Identities = 43/70 (61%), Positives = 55/70 (78%)
 Frame = +2

Query: 2   KDGKLDHDEIGKWILPLDHNHVTAETNHLMEQTDANKDGYLTKVEILEKYDVFVGSQATD 181
           +DGK+D +E   WILP D++H  AE  HL+ ++D NKDG LTK EI++KYD+FVGSQATD
Sbjct: 245 RDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATD 304

Query: 182 FGDALTRHAE 211
           FG+AL RH E
Sbjct: 305 FGEALVRHDE 314
>sp|O35887|CALU_MOUSE Calumenin precursor (Crocalbin)
          Length = 315

 Score = 96.7 bits (239), Expect = 2e-20
 Identities = 43/70 (61%), Positives = 55/70 (78%)
 Frame = +2

Query: 2   KDGKLDHDEIGKWILPLDHNHVTAETNHLMEQTDANKDGYLTKVEILEKYDVFVGSQATD 181
           +DGK+D +E   WILP D++H  AE  HL+ ++D NKDG LTK EI++KYD+FVGSQATD
Sbjct: 245 RDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATD 304

Query: 182 FGDALTRHAE 211
           FG+AL RH E
Sbjct: 305 FGEALVRHDE 314
>sp|O35783|CALU_RAT Calumenin precursor (Crocalbin) (CBP-50)
          Length = 315

 Score = 94.7 bits (234), Expect = 6e-20
 Identities = 42/70 (60%), Positives = 55/70 (78%)
 Frame = +2

Query: 2   KDGKLDHDEIGKWILPLDHNHVTAETNHLMEQTDANKDGYLTKVEILEKYDVFVGSQATD 181
           +DGK+D +E   WILP D++H  AE  HL+ ++D +KDG LTK EI++KYD+FVGSQATD
Sbjct: 245 RDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQDKDGKLTKEEIVDKYDLFVGSQATD 304

Query: 182 FGDALTRHAE 211
           FG+AL RH E
Sbjct: 305 FGEALVRHDE 314
>sp|Q15293|RCN1_HUMAN Reticulocalbin-1 precursor
          Length = 331

 Score = 87.8 bits (216), Expect = 7e-18
 Identities = 43/72 (59%), Positives = 55/72 (76%), Gaps = 1/72 (1%)
 Frame = +2

Query: 2   KDGKLDHDEIGKWILPLDHNHVTAETNHLMEQTDANKDGYLTKVEILEKYDVFVGSQATD 181
           KDGKLD DEI  WILP D++H  AE  HL+ ++D NKD  LTK EILE +++FVGSQAT+
Sbjct: 260 KDGKLDKDEIRHWILPQDYDHAQAEARHLVYESDKNKDEKLTKEEILENWNMFVGSQATN 319

Query: 182 FGDALTR-HAEL 214
           +G+ LT+ H EL
Sbjct: 320 YGEDLTKNHDEL 331
>sp|Q05186|RCN1_MOUSE Reticulocalbin-1 precursor
          Length = 325

 Score = 87.0 bits (214), Expect = 1e-17
 Identities = 42/72 (58%), Positives = 55/72 (76%), Gaps = 1/72 (1%)
 Frame = +2

Query: 2   KDGKLDHDEIGKWILPLDHNHVTAETNHLMEQTDANKDGYLTKVEILEKYDVFVGSQATD 181
           KDGKLD DEI  WILP D++H  AE  HL+ ++D NKD  LTK EIL+ +++FVGSQAT+
Sbjct: 254 KDGKLDKDEIRHWILPQDYDHAQAEARHLVYESDKNKDEMLTKEEILDNWNMFVGSQATN 313

Query: 182 FGDALTR-HAEL 214
           +G+ LT+ H EL
Sbjct: 314 YGEDLTKNHDEL 325
>sp|O93434|RCN1_FUGRU Reticulocalbin-1 precursor
          Length = 322

 Score = 84.0 bits (206), Expect = 1e-16
 Identities = 40/72 (55%), Positives = 55/72 (76%), Gaps = 1/72 (1%)
 Frame = +2

Query: 2   KDGKLDHDEIGKWILPLDHNHVTAETNHLMEQTDANKDGYLTKVEILEKYDVFVGSQATD 181
           KDGK+D DEI  WI+P D++H  AE  HL+ ++D +KD  LTK EIL+ +++FVGSQAT+
Sbjct: 251 KDGKMDLDEIRHWIMPQDYDHAQAEARHLVYESDKDKDQMLTKEEILDNWNMFVGSQATN 310

Query: 182 FGDALTR-HAEL 214
           +G+ LTR H EL
Sbjct: 311 YGEDLTRNHDEL 322
>sp|Q8BH97|RCN3_MOUSE Reticulocalbin-3 precursor
          Length = 328

 Score = 82.4 bits (202), Expect = 3e-16
 Identities = 35/68 (51%), Positives = 50/68 (73%)
 Frame = +2

Query: 2   KDGKLDHDEIGKWILPLDHNHVTAETNHLMEQTDANKDGYLTKVEILEKYDVFVGSQATD 181
           KDG+LD  E+G W+LP   +    E NHL+ ++D +KDG L+K EIL  +++FVGSQAT+
Sbjct: 257 KDGRLDGSEVGYWVLPPSQDQPLVEANHLLHESDTDKDGRLSKAEILSNWNMFVGSQATN 316

Query: 182 FGDALTRH 205
           +G+ LTRH
Sbjct: 317 YGEDLTRH 324
>sp|Q96D15|RCN3_HUMAN Reticulocalbin-3 precursor (EF-hand calcium-binding protein RLP49)
          Length = 328

 Score = 80.1 bits (196), Expect = 1e-15
 Identities = 35/68 (51%), Positives = 49/68 (72%)
 Frame = +2

Query: 2   KDGKLDHDEIGKWILPLDHNHVTAETNHLMEQTDANKDGYLTKVEILEKYDVFVGSQATD 181
           KDG LD  E+G W+LP   +    E NHL+ ++D +KDG L+K EIL  +++FVGSQAT+
Sbjct: 257 KDGHLDGSEVGHWVLPPAQDQPLVEANHLLHESDTDKDGRLSKAEILGNWNMFVGSQATN 316

Query: 182 FGDALTRH 205
           +G+ LTRH
Sbjct: 317 YGEDLTRH 324
>sp|Q62703|RCN2_RAT Reticulocalbin-2 precursor (Calcium-binding protein ERC-55)
           (Taipoxin-associated calcium-binding protein 49)
           (TCBP-49)
          Length = 320

 Score = 56.6 bits (135), Expect = 2e-08
 Identities = 26/64 (40%), Positives = 40/64 (62%)
 Frame = +2

Query: 5   DGKLDHDEIGKWILPLDHNHVTAETNHLMEQTDANKDGYLTKVEILEKYDVFVGSQATDF 184
           DG+LD  E+  W++P +      E  HL+++ D N D  L++ EILE  D+F+ S+ATD+
Sbjct: 247 DGRLDPQELLSWVVPNNQGIAQEEALHLIDEMDLNSDKKLSEEEILENQDLFLTSEATDY 306

Query: 185 GDAL 196
           G  L
Sbjct: 307 GRQL 310

 Score = 28.1 bits (61), Expect = 6.6
 Identities = 16/62 (25%), Positives = 29/62 (46%)
 Frame = +2

Query: 5   DGKLDHDEIGKWILPLDHNHVTAETNHLMEQTDANKDGYLTKVEILEKYDVFVGSQATDF 184
           DG L  +E+ +WI     ++   E      + D N DG +T     ++Y+V +  +  DF
Sbjct: 81  DGFLTENELSQWIQMSFKHYAMQEAKQQFVEYDKNSDGTVT----WDEYNVQMYDRVIDF 136

Query: 185 GD 190
            +
Sbjct: 137 DE 138
>sp|Q8BP92|RCN2_MOUSE Reticulocalbin-2 precursor (Taipoxin-associated calcium-binding
           protein 49) (TCBP-49)
          Length = 320

 Score = 56.6 bits (135), Expect = 2e-08
 Identities = 26/64 (40%), Positives = 40/64 (62%)
 Frame = +2

Query: 5   DGKLDHDEIGKWILPLDHNHVTAETNHLMEQTDANKDGYLTKVEILEKYDVFVGSQATDF 184
           DG+LD  E+  W++P +      E  HL+++ D N D  L++ EILE  D+F+ S+ATD+
Sbjct: 247 DGRLDPQELLSWVVPNNQGIAQEEALHLIDEMDLNSDKKLSEEEILENQDLFLTSEATDY 306

Query: 185 GDAL 196
           G  L
Sbjct: 307 GRQL 310

 Score = 27.7 bits (60), Expect = 8.6
 Identities = 15/62 (24%), Positives = 29/62 (46%)
 Frame = +2

Query: 5   DGKLDHDEIGKWILPLDHNHVTAETNHLMEQTDANKDGYLTKVEILEKYDVFVGSQATDF 184
           DG L  +E+ +WI     ++   E      + D N DG +T     ++Y++ +  +  DF
Sbjct: 81  DGFLTENELSQWIQMSFKHYAMQEAKQQFVEYDKNSDGAVT----WDEYNIQMYDRVIDF 136

Query: 185 GD 190
            +
Sbjct: 137 DE 138
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 45,192,885
Number of Sequences: 369166
Number of extensions: 841664
Number of successful extensions: 2203
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2140
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2203
length of database: 68,354,980
effective HSP length: 96
effective length of database: 50,620,420
effective search space used: 1721094280
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)