Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_010_O16-2
(372 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P22314|UBE1_HUMAN Ubiquitin-activating enzyme E1 (A1S9 p... 63 2e-10
sp|Q02053|UBE1_MOUSE Ubiquitin-activating enzyme E1 1 63 2e-10
sp|Q29504|UBE1_RABIT Ubiquitin-activating enzyme E1 63 2e-10
sp|P22515|UBA1_YEAST Ubiquitin-activating enzyme E1 1 61 9e-10
sp|P52495|UBA1_CANAL Ubiquitin-activating enzyme E1 1 61 9e-10
sp|P31254|UBE1Y_MOUSE Ubiquitin-activating enzyme E1 Y 54 1e-07
sp|P20973|UBE11_WHEAT Ubiquitin-activating enzyme E1 1 50 1e-06
sp|P31251|UBE12_WHEAT Ubiquitin-activating enzyme E1 2 50 1e-06
sp|P31252|UBE13_WHEAT Ubiquitin-activating enzyme E1 3 49 5e-06
sp|P31253|UBE1X_MOUSE Ubiquitin-activating enzyme E1 X 46 2e-05
>sp|P22314|UBE1_HUMAN Ubiquitin-activating enzyme E1 (A1S9 protein)
Length = 1058
Score = 63.2 bits (152), Expect = 2e-10
Identities = 30/102 (29%), Positives = 57/102 (55%), Gaps = 6/102 (5%)
Frame = -3
Query: 370 WDNFEIHG------DVTLREFLQFIKTNHNLDVTCVTYKTGVLYALWFDKEYIEDNMSRL 209
WD FE+ G ++TL++FL + KT H L++T ++ +LY+ + +++ + +
Sbjct: 955 WDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQP 1014
Query: 208 ITDIIRNKIKLVISESESVIPLYISVEDENGEDIDGVPVVRY 83
+T+I+ K + + L + DE+GED++ VP VRY
Sbjct: 1015 MTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVE-VPYVRY 1055
>sp|Q02053|UBE1_MOUSE Ubiquitin-activating enzyme E1 1
Length = 1058
Score = 63.2 bits (152), Expect = 2e-10
Identities = 30/102 (29%), Positives = 57/102 (55%), Gaps = 6/102 (5%)
Frame = -3
Query: 370 WDNFEIHG------DVTLREFLQFIKTNHNLDVTCVTYKTGVLYALWFDKEYIEDNMSRL 209
WD FE+ G ++TL++FL + KT H L++T ++ +LY+ + +++ + +
Sbjct: 955 WDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQP 1014
Query: 208 ITDIIRNKIKLVISESESVIPLYISVEDENGEDIDGVPVVRY 83
+T+I+ K + + L + DE+GED++ VP VRY
Sbjct: 1015 MTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVE-VPYVRY 1055
>sp|Q29504|UBE1_RABIT Ubiquitin-activating enzyme E1
Length = 1058
Score = 63.2 bits (152), Expect = 2e-10
Identities = 30/102 (29%), Positives = 57/102 (55%), Gaps = 6/102 (5%)
Frame = -3
Query: 370 WDNFEIHG------DVTLREFLQFIKTNHNLDVTCVTYKTGVLYALWFDKEYIEDNMSRL 209
WD FE+ G ++TL++FL + KT H L++T ++ +LY+ + +++ + +
Sbjct: 955 WDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQP 1014
Query: 208 ITDIIRNKIKLVISESESVIPLYISVEDENGEDIDGVPVVRY 83
+T+I+ K + + L + DE+GED++ VP VRY
Sbjct: 1015 MTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVE-VPYVRY 1055
>sp|P22515|UBA1_YEAST Ubiquitin-activating enzyme E1 1
Length = 1024
Score = 60.8 bits (146), Expect = 9e-10
Identities = 28/97 (28%), Positives = 56/97 (57%)
Frame = -3
Query: 370 WDNFEIHGDVTLREFLQFIKTNHNLDVTCVTYKTGVLYALWFDKEYIEDNMSRLITDIIR 191
WD F+I GD+ L + ++ + + L++T ++Y +LYA +F + +++ ++ IT +++
Sbjct: 928 WDRFDIKGDIKLSDLIEHFEKDEGLEITMLSYGVSLLYASFFPPKKLKERLNLPITQLVK 987
Query: 190 NKIKLVISESESVIPLYISVEDENGEDIDGVPVVRYH 80
K I S + L I +D+ GED++ VP + H
Sbjct: 988 LVTKKDIPAHVSTMILEICADDKEGEDVE-VPFITIH 1023
>sp|P52495|UBA1_CANAL Ubiquitin-activating enzyme E1 1
Length = 205
Score = 60.8 bits (146), Expect = 9e-10
Identities = 28/94 (29%), Positives = 52/94 (55%)
Frame = -3
Query: 370 WDNFEIHGDVTLREFLQFIKTNHNLDVTCVTYKTGVLYALWFDKEYIEDNMSRLITDIIR 191
WD FE++GD+TL+E L + L ++ ++Y +LYA +F + ++D + +T +I+
Sbjct: 109 WDRFELNGDITLQELLDHFEKEEGLTISMLSYGVSLLYASFFPPKKVKDRLGLKLTSLIK 168
Query: 190 NKIKLVISESESVIPLYISVEDENGEDIDGVPVV 89
K + + I +DE GED++ VP +
Sbjct: 169 EVSKKEVPSHVKNLIFEICCDDEEGEDVE-VPYI 201
>sp|P31254|UBE1Y_MOUSE Ubiquitin-activating enzyme E1 Y
Length = 442
Score = 53.5 bits (127), Expect = 1e-07
Identities = 26/102 (25%), Positives = 55/102 (53%), Gaps = 6/102 (5%)
Frame = -3
Query: 370 WDNFEIHG------DVTLREFLQFIKTNHNLDVTCVTYKTGVLYALWFDKEYIEDNMSRL 209
WD F++ G ++TL++FL + KT H L+V ++ +LY+++ +++ + +
Sbjct: 339 WDRFDVQGLQPSGEEMTLKQFLDYFKTEHKLEVIMLSQGVSMLYSVFMPASKLKERLDQP 398
Query: 208 ITDIIRNKIKLVISESESVIPLYISVEDENGEDIDGVPVVRY 83
+T+I+ K + + + ++G+DI+ VP VRY
Sbjct: 399 MTEIVSCVSKQKLGHHVKSLVFELCCNSDSGDDIE-VPYVRY 439
>sp|P20973|UBE11_WHEAT Ubiquitin-activating enzyme E1 1
Length = 1051
Score = 50.4 bits (119), Expect = 1e-06
Identities = 25/98 (25%), Positives = 54/98 (55%)
Frame = -3
Query: 370 WDNFEIHGDVTLREFLQFIKTNHNLDVTCVTYKTGVLYALWFDKEYIEDNMSRLITDIIR 191
WD + + G++TLRE L+++K L+ ++ T +LY F + ++ + R + D+ R
Sbjct: 957 WDRWTVTGNITLRELLEWLK-EKGLNAYSISCGTSLLYNSMFPRH--KERLDRKVVDVAR 1013
Query: 190 NKIKLVISESESVIPLYISVEDENGEDIDGVPVVRYHF 77
K+ + + + ++ ED++ D+D +P+V +F
Sbjct: 1014 EVAKMEVPSYRRHLDVVVACEDDDDNDVD-IPLVSVYF 1050
>sp|P31251|UBE12_WHEAT Ubiquitin-activating enzyme E1 2
Length = 1051
Score = 50.4 bits (119), Expect = 1e-06
Identities = 25/98 (25%), Positives = 54/98 (55%)
Frame = -3
Query: 370 WDNFEIHGDVTLREFLQFIKTNHNLDVTCVTYKTGVLYALWFDKEYIEDNMSRLITDIIR 191
WD + + G++TLRE L+++K L+ ++ T +LY F + ++ + R + D+ R
Sbjct: 957 WDRWTVTGNITLRELLEWLK-EKGLNAYSISCGTSLLYNSMFPRH--KERLDRKVVDVAR 1013
Query: 190 NKIKLVISESESVIPLYISVEDENGEDIDGVPVVRYHF 77
K+ + + + ++ ED++ D+D +P+V +F
Sbjct: 1014 EVAKMEVPSYRRHLDVVVACEDDDDNDVD-IPLVSVYF 1050
>sp|P31252|UBE13_WHEAT Ubiquitin-activating enzyme E1 3
Length = 1053
Score = 48.5 bits (114), Expect = 5e-06
Identities = 27/98 (27%), Positives = 53/98 (54%)
Frame = -3
Query: 370 WDNFEIHGDVTLREFLQFIKTNHNLDVTCVTYKTGVLYALWFDKEYIEDNMSRLITDIIR 191
WD + + G++TL E LQ+ + L ++ T +LY F + +D +++ + DI R
Sbjct: 959 WDRWSVQGNLTLAELLQWF-ADKGLTAYSISCGTSLLYNNMFARH--KDRLTKKVVDIAR 1015
Query: 190 NKIKLVISESESVIPLYISVEDENGEDIDGVPVVRYHF 77
K+ + E + + ++ EDE+ D+D +P+V +F
Sbjct: 1016 EVAKVDVPEYRRHLDIGVACEDEDENDVD-IPLVSVYF 1052
>sp|P31253|UBE1X_MOUSE Ubiquitin-activating enzyme E1 X
Length = 450
Score = 46.2 bits (108), Expect = 2e-05
Identities = 18/65 (27%), Positives = 39/65 (60%), Gaps = 6/65 (9%)
Frame = -3
Query: 370 WDNFEIHG------DVTLREFLQFIKTNHNLDVTCVTYKTGVLYALWFDKEYIEDNMSRL 209
WD FE+ G ++TL++FL + KT H L++T ++ +LY+ + +++ + +
Sbjct: 341 WDRFEVQGVQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQP 400
Query: 208 ITDII 194
+T+I+
Sbjct: 401 MTEIV 405
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 36,301,309
Number of Sequences: 369166
Number of extensions: 605643
Number of successful extensions: 1613
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1594
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1611
length of database: 68,354,980
effective HSP length: 90
effective length of database: 51,728,830
effective search space used: 1707051390
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)