Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_010_N19
(806 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P40926|MDHM_HUMAN Malate dehydrogenase, mitochondrial pr... 359 4e-99
sp|P00346|MDHM_PIG Malate dehydrogenase, mitochondrial prec... 359 4e-99
sp|P04636|MDHM_RAT Malate dehydrogenase, mitochondrial prec... 357 2e-98
sp|P08249|MDHM_MOUSE Malate dehydrogenase, mitochondrial pr... 357 2e-98
sp|O02640|MDHM_CAEEL Probable malate dehydrogenase, mitocho... 323 3e-88
sp|Q42972|MDHG_ORYSA Malate dehydrogenase, glyoxysomal prec... 307 3e-83
sp|O82399|MDHI_ARATH Probable malate dehydrogenase, glyoxys... 305 1e-82
sp|P19446|MDHG_CITLA Malate dehydrogenase, glyoxysomal prec... 301 1e-81
sp|P46488|MDHG_CUCSA Malate dehydrogenase, glyoxysomal prec... 301 1e-81
sp|Q9ZP05|MDHG_ARATH Malate dehydrogenase, glyoxysomal prec... 297 2e-80
>sp|P40926|MDHM_HUMAN Malate dehydrogenase, mitochondrial precursor
Length = 338
Score = 359 bits (922), Expect = 4e-99
Identities = 179/258 (69%), Positives = 209/258 (81%)
Frame = +2
Query: 29 IARSFSTCQTLNAKVAVLGASGGIGQPMSLLLKKSSAVDRLALYDIAHVKGVAADLSHIE 208
+ RSFST NAKVAVLGASGGIGQP+SLLLK S V RL LYDIAH GVAADLSHIE
Sbjct: 13 LRRSFSTSAQNNAKVAVLGASGGIGQPLSLLLKNSPLVSRLTLYDIAHTPGVAADLSHIE 72
Query: 209 TPAVVNGYLGPSELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQAC 388
T A V GYLGP +L +CLK DVVVIPAGVPRKPGMTRDDLFNTNA+IVA L CAQ C
Sbjct: 73 TKAAVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHC 132
Query: 389 PKAIICIITNPVNSTVPIAAEILKKAGCYDPKRLFGVTTLDIVRSNTFISEAKHLDVTKM 568
P+A+IC+I NPVNST+PI AE+ KK G Y+P ++FGVTTLDIVR+NTF++E K LD ++
Sbjct: 133 PEAMICVIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARV 192
Query: 569 SVPVIGGHSGVTIVPVISQAIPSVSFPESEISKLTIRIQNAGTEVVEAKAGAGSATLSMA 748
+VPVIGGH+G TI+P+ISQ P V FP+ +++ LT RIQ AGTEVV+AKAGAGSATLSMA
Sbjct: 193 NVPVIGGHAGKTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMA 252
Query: 749 YAGVRFVKRILAAMKGNK 802
YAG RFV ++ AM G +
Sbjct: 253 YAGARFVFSLVDAMNGKE 270
>sp|P00346|MDHM_PIG Malate dehydrogenase, mitochondrial precursor
Length = 338
Score = 359 bits (922), Expect = 4e-99
Identities = 181/258 (70%), Positives = 208/258 (80%)
Frame = +2
Query: 29 IARSFSTCQTLNAKVAVLGASGGIGQPMSLLLKKSSAVDRLALYDIAHVKGVAADLSHIE 208
+ RSFST NAKVAVLGASGGIGQP+SLLLK S V RL LYDIAH GVAADLSHIE
Sbjct: 13 LRRSFSTSXQNNAKVAVLGASGGIGQPLSLLLKNSPLVSRLTLYDIAHTPGVAADLSHIE 72
Query: 209 TPAVVNGYLGPSELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQAC 388
T A V GYLGP +L +CLK DVVVIPAGVPRKPGMTRDDLFNTNA+IVA L CAQ C
Sbjct: 73 TRATVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHC 132
Query: 389 PKAIICIITNPVNSTVPIAAEILKKAGCYDPKRLFGVTTLDIVRSNTFISEAKHLDVTKM 568
P A+ICII+NPVNST+PI AE+ KK G Y+P ++FGVTTLDIVR+N F++E K LD ++
Sbjct: 133 PDAMICIISNPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANAFVAELKGLDPARV 192
Query: 569 SVPVIGGHSGVTIVPVISQAIPSVSFPESEISKLTIRIQNAGTEVVEAKAGAGSATLSMA 748
SVPVIGGH+G TI+P+ISQ P V FP+ ++S LT RIQ AGTEVV+AKAGAGSATLSMA
Sbjct: 193 SVPVIGGHAGKTIIPLISQCTPKVDFPQDQLSTLTGRIQEAGTEVVKAKAGAGSATLSMA 252
Query: 749 YAGVRFVKRILAAMKGNK 802
YAG RFV ++ AM G +
Sbjct: 253 YAGARFVFSLVDAMNGKE 270
>sp|P04636|MDHM_RAT Malate dehydrogenase, mitochondrial precursor
Length = 338
Score = 357 bits (917), Expect = 2e-98
Identities = 179/258 (69%), Positives = 210/258 (81%)
Frame = +2
Query: 29 IARSFSTCQTLNAKVAVLGASGGIGQPMSLLLKKSSAVDRLALYDIAHVKGVAADLSHIE 208
+ RSFST NAKVAVLGASGGIGQP+SLLLK S V RL LYDIAH GVAADLSHIE
Sbjct: 13 LRRSFSTSAQNNAKVAVLGASGGIGQPLSLLLKNSPLVSRLTLYDIAHTPGVAADLSHIE 72
Query: 209 TPAVVNGYLGPSELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQAC 388
T A V GYLGP +L +CLK DVVVIPAGVPRKPGMTRDDLFNTNA+IVA L CAQ C
Sbjct: 73 TRANVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHC 132
Query: 389 PKAIICIITNPVNSTVPIAAEILKKAGCYDPKRLFGVTTLDIVRSNTFISEAKHLDVTKM 568
P+A+ICII+NPVNST+PI AE+ KK G Y+P ++FGVTTLDIVR+NTF++E K LD ++
Sbjct: 133 PEAMICIISNPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARV 192
Query: 569 SVPVIGGHSGVTIVPVISQAIPSVSFPESEISKLTIRIQNAGTEVVEAKAGAGSATLSMA 748
+VPVIGGH+G TI+P+ISQ P V FP+ +++ LT +IQ AGTEVV+AKAGAGSATLSMA
Sbjct: 193 NVPVIGGHAGKTIIPLISQCTPKVDFPQDQLATLTGKIQEAGTEVVKAKAGAGSATLSMA 252
Query: 749 YAGVRFVKRILAAMKGNK 802
YAG RFV ++ AM G +
Sbjct: 253 YAGARFVFSLVDAMNGKE 270
>sp|P08249|MDHM_MOUSE Malate dehydrogenase, mitochondrial precursor
Length = 338
Score = 357 bits (917), Expect = 2e-98
Identities = 179/256 (69%), Positives = 208/256 (81%)
Frame = +2
Query: 29 IARSFSTCQTLNAKVAVLGASGGIGQPMSLLLKKSSAVDRLALYDIAHVKGVAADLSHIE 208
+ RSFST NAKVAVLGASGGIGQP+SLLLK S V RL LYDIAH GVAADLSHIE
Sbjct: 13 LRRSFSTSAQNNAKVAVLGASGGIGQPLSLLLKNSPLVSRLTLYDIAHTPGVAADLSHIE 72
Query: 209 TPAVVNGYLGPSELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQAC 388
T A V GYLGP +L +CLK DVVVIPAGVPRKPGMTRDDLFNTNA+IVA L CAQ C
Sbjct: 73 TRANVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHC 132
Query: 389 PKAIICIITNPVNSTVPIAAEILKKAGCYDPKRLFGVTTLDIVRSNTFISEAKHLDVTKM 568
P+A++CII NPVNST+PI AE+ KK G Y+P ++FGVTTLDIVR+NTF++E K LD ++
Sbjct: 133 PEAMVCIIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARV 192
Query: 569 SVPVIGGHSGVTIVPVISQAIPSVSFPESEISKLTIRIQNAGTEVVEAKAGAGSATLSMA 748
+VPVIGGH+G TI+P+ISQ P V FP+ +++ LT RIQ AGTEVV+AKAGAGSATLSMA
Sbjct: 193 NVPVIGGHAGKTIIPLISQCTPKVDFPQDQLATLTGRIQEAGTEVVKAKAGAGSATLSMA 252
Query: 749 YAGVRFVKRILAAMKG 796
YAG RFV ++ AM G
Sbjct: 253 YAGARFVFSLVDAMNG 268
>sp|O02640|MDHM_CAEEL Probable malate dehydrogenase, mitochondrial precursor
Length = 341
Score = 323 bits (829), Expect = 3e-88
Identities = 163/245 (66%), Positives = 194/245 (79%)
Frame = +2
Query: 68 KVAVLGASGGIGQPMSLLLKKSSAVDRLALYDIAHVKGVAADLSHIETPAVVNGYLGPSE 247
KVA+LGA+GGIGQP+ LLLK+ V LALYD+ + GVAADLSHI++ A V + GP E
Sbjct: 30 KVALLGAAGGIGQPLGLLLKQDPLVAHLALYDVVNTPGVAADLSHIDSNAKVTAHTGPKE 89
Query: 248 LLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAIICIITNPVN 427
L ++N+DV+VIPAGVPRKPGMTRDDLFNTNA IV DL A+A PKA+I IITNPVN
Sbjct: 90 LYAAVENADVIVIPAGVPRKPGMTRDDLFNTNAGIVRDLAAVIAKASPKALIAIITNPVN 149
Query: 428 STVPIAAEILKKAGCYDPKRLFGVTTLDIVRSNTFISEAKHLDVTKMSVPVIGGHSGVTI 607
STVPIA+E+LKKAG YDPKR+FGVTTLD+VRS F+SE K D +K VPV+GGH+G+TI
Sbjct: 150 STVPIASEVLKKAGVYDPKRVFGVTTLDVVRSQAFVSELKGHDASKTVVPVVGGHAGITI 209
Query: 608 VPVISQAIPSVSFPESEISKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAA 787
+P++SQ PS F E EISKLT RIQ+AGTEVV AKAGAGSATLSMA AG RF ++
Sbjct: 210 IPLLSQVKPSTKFSEEEISKLTPRIQDAGTEVVNAKAGAGSATLSMALAGARFANALVRG 269
Query: 788 MKGNK 802
+KG K
Sbjct: 270 IKGEK 274
>sp|Q42972|MDHG_ORYSA Malate dehydrogenase, glyoxysomal precursor
Length = 356
Score = 307 bits (786), Expect = 3e-83
Identities = 157/256 (61%), Positives = 190/256 (74%), Gaps = 4/256 (1%)
Frame = +2
Query: 44 STCQTLNA----KVAVLGASGGIGQPMSLLLKKSSAVDRLALYDIAHVKGVAADLSHIET 211
S C+ A KVA+LGASGGIGQP++LL+K + V L LYD+ + GV AD+SH+ T
Sbjct: 34 SNCRAKGAAPGFKVAILGASGGIGQPLALLMKMNPLVSVLHLYDVVNTPGVTADISHMNT 93
Query: 212 PAVVNGYLGPSELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACP 391
AVV G+LG +L L D+V+IPAGVPRKPGMTRDDLFN NA IV L G A+ CP
Sbjct: 94 GAVVRGFLGQPQLENALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCP 153
Query: 392 KAIICIITNPVNSTVPIAAEILKKAGCYDPKRLFGVTTLDIVRSNTFISEAKHLDVTKMS 571
AI+ +I+NPVNSTVPIAAE+ KKAG YDPKRL GVTTLD+VR+NTF++E LD ++
Sbjct: 154 NAIVNVISNPVNSTVPIAAEVFKKAGTYDPKRLLGVTTLDVVRANTFVAEVLGLDPRDVN 213
Query: 572 VPVIGGHSGVTIVPVISQAIPSVSFPESEISKLTIRIQNAGTEVVEAKAGAGSATLSMAY 751
VPVIGGH+GVTI+P++SQ P SF EIS LT RIQN GTEVVEAKAGAGSATLSMAY
Sbjct: 214 VPVIGGHAGVTILPLLSQVNPPCSFTSEEISYLTTRIQNGGTEVVEAKAGAGSATLSMAY 273
Query: 752 AGVRFVKRILAAMKGN 799
A +F L ++G+
Sbjct: 274 AASKFADACLRGLRGD 289
>sp|O82399|MDHI_ARATH Probable malate dehydrogenase, glyoxysomal precursor
Length = 354
Score = 305 bits (780), Expect = 1e-82
Identities = 151/244 (61%), Positives = 188/244 (77%)
Frame = +2
Query: 68 KVAVLGASGGIGQPMSLLLKKSSAVDRLALYDIAHVKGVAADLSHIETPAVVNGYLGPSE 247
KVA+LGA+GGIGQP+++L+K + V L LYD+A+ GV AD+SH++T AVV G+LG +
Sbjct: 44 KVAILGAAGGIGQPLAMLMKMNPLVSVLHLYDVANAPGVTADISHMDTSAVVRGFLGQPQ 103
Query: 248 LLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAIICIITNPVN 427
L E L D+V+IPAGVPRKPGMTRDDLFN NA IV L A+ CPKAI+ II+NPVN
Sbjct: 104 LEEALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLSEAIAKCCPKAIVNIISNPVN 163
Query: 428 STVPIAAEILKKAGCYDPKRLFGVTTLDIVRSNTFISEAKHLDVTKMSVPVIGGHSGVTI 607
STVPIAAE+ KKAG +DPK+L GVT LD+VR+NTF++E LD ++ VPV+GGH+GVTI
Sbjct: 164 STVPIAAEVFKKAGTFDPKKLMGVTMLDVVRANTFVAEVMSLDpreVEVPVVGGHAGVTI 223
Query: 608 VPVISQAIPSVSFPESEISKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAA 787
+P++SQ P SF + EI LT RIQN GTEVVEAKAGAGSATLSMAYA V F L
Sbjct: 224 LPLLSQVKPPCSFTQKEIEYLTDRIQNGGTEVVEAKAGAGSATLSMAYAAVEFADACLRG 283
Query: 788 MKGN 799
++G+
Sbjct: 284 LRGD 287
>sp|P19446|MDHG_CITLA Malate dehydrogenase, glyoxysomal precursor
Length = 356
Score = 301 bits (772), Expect = 1e-81
Identities = 148/244 (60%), Positives = 187/244 (76%)
Frame = +2
Query: 68 KVAVLGASGGIGQPMSLLLKKSSAVDRLALYDIAHVKGVAADLSHIETPAVVNGYLGPSE 247
KVA+LGA+GGIGQP+++L+K + V L LYD+ + GV AD+SH++T AVV G+LG +
Sbjct: 46 KVAILGAAGGIGQPLAMLMKMNPLVSVLHLYDVVNAPGVTADISHMDTGAVVRGFLGQQQ 105
Query: 248 LLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAIICIITNPVN 427
L L D++++PAGVPRKPGMTRDDLF NA IV L G A+ CP+AI+ +I+NPVN
Sbjct: 106 LEAALTGMDLIIVPAGVPRKPGMTRDDLFKINAGIVKTLCEGIAKCCPRAIVNLISNPVN 165
Query: 428 STVPIAAEILKKAGCYDPKRLFGVTTLDIVRSNTFISEAKHLDVTKMSVPVIGGHSGVTI 607
STVPIAAE+ KKAG YDPKRL GVT LD+VR+NTF++E LD + VPV+GGH+GVTI
Sbjct: 166 STVPIAAEVFKKAGTYDPKRLLGVTMLDVVRANTFVAEVLGLDPRDVDVPVVGGHAGVTI 225
Query: 608 VPVISQAIPSVSFPESEISKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAA 787
+P++SQ P SF + EIS LT RIQN GTEVVEAKAGAGSATLSMAYA V+F L
Sbjct: 226 LPLLSQVKPPSSFTQEEISYLTDRIQNGGTEVVEAKAGAGSATLSMAYAAVKFADACLRG 285
Query: 788 MKGN 799
++G+
Sbjct: 286 LRGD 289
>sp|P46488|MDHG_CUCSA Malate dehydrogenase, glyoxysomal precursor
Length = 356
Score = 301 bits (771), Expect = 1e-81
Identities = 150/244 (61%), Positives = 187/244 (76%)
Frame = +2
Query: 68 KVAVLGASGGIGQPMSLLLKKSSAVDRLALYDIAHVKGVAADLSHIETPAVVNGYLGPSE 247
KVA+LGA+GGIGQP+++L+K + V L LYD+ + GV AD+SH++T AVV G+LG +
Sbjct: 46 KVAILGAAGGIGQPLAMLMKMNPLVSVLHLYDVVNAPGVTADISHMDTGAVVRGFLGQQQ 105
Query: 248 LLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAIICIITNPVN 427
L L D+VVIPAGVPRKPGMTRDDLF NA IV L G A+ CP AI+ +I+NPVN
Sbjct: 106 LERALTGMDLVVIPAGVPRKPGMTRDDLFKINAGIVKTLCEGIAKCCPTAIVNLISNPVN 165
Query: 428 STVPIAAEILKKAGCYDPKRLFGVTTLDIVRSNTFISEAKHLDVTKMSVPVIGGHSGVTI 607
STVPIAAE+ KKAG YDPKRL GVT LD+VR+NTF++E LD ++VPV+GGH+GVTI
Sbjct: 166 STVPIAAEVFKKAGTYDPKRLLGVTMLDVVRANTFVAEVLGLDPRDVNVPVVGGHAGVTI 225
Query: 608 VPVISQAIPSVSFPESEISKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAA 787
+P++SQ P SF + EI+ LT RIQN GTEVVEAKAGAGSATLSMAYA V+F L
Sbjct: 226 LPLLSQVKPPSSFTQEEINYLTDRIQNGGTEVVEAKAGAGSATLSMAYAAVKFADACLRG 285
Query: 788 MKGN 799
++G+
Sbjct: 286 LRGD 289
>sp|Q9ZP05|MDHG_ARATH Malate dehydrogenase, glyoxysomal precursor (mbNAD-MDH)
Length = 354
Score = 297 bits (761), Expect = 2e-80
Identities = 147/244 (60%), Positives = 184/244 (75%)
Frame = +2
Query: 68 KVAVLGASGGIGQPMSLLLKKSSAVDRLALYDIAHVKGVAADLSHIETPAVVNGYLGPSE 247
KVA+LGA+GGIGQ +SLL+K + V L LYD+ + GV AD+SH++T AVV G+LG +
Sbjct: 44 KVAILGAAGGIGQSLSLLMKMNPLVSLLHLYDVVNAPGVTADVSHMDTGAVVRGFLGAKQ 103
Query: 248 LLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAIICIITNPVN 427
L + L D+V+IPAG+PRKPGMTRDDLF NA IV L G A+ CP AI+ +I+NPVN
Sbjct: 104 LEDALTGMDLVIIPAGIPRKPGMTRDDLFKINAGIVKTLCEGVAKCCPNAIVNLISNPVN 163
Query: 428 STVPIAAEILKKAGCYDPKRLFGVTTLDIVRSNTFISEAKHLDVTKMSVPVIGGHSGVTI 607
STVPIAAE+ KKAG YDPK+L GVTTLD+ R+NTF++E LD ++ VPV+GGH+GVTI
Sbjct: 164 STVPIAAEVFKKAGTYDPKKLLGVTTLDVARANTFVAEVLGLDpreVDVPVVGGHAGVTI 223
Query: 608 VPVISQAIPSVSFPESEISKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAA 787
+P++SQ P SF EI LT RIQN GTEVVEAKAGAGSATLSMAYA +F L
Sbjct: 224 LPLLSQVKPPSSFTPQEIEYLTNRIQNGGTEVVEAKAGAGSATLSMAYAAAKFADACLRG 283
Query: 788 MKGN 799
++G+
Sbjct: 284 LRGD 287
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 91,180,137
Number of Sequences: 369166
Number of extensions: 1870081
Number of successful extensions: 5888
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5501
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5705
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7666799535
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)