Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_010_M05
(711 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q92499|DDX1_HUMAN ATP-dependent helicase DDX1 (DEAD-box ... 273 4e-73
sp|Q91VR5|DDX1_MOUSE ATP-dependent helicase DDX1 (DEAD-box ... 273 4e-73
sp|Q641Y8|DDX1_RAT ATP-dependent helicase DDX1 (DEAD-box pr... 272 6e-73
sp|Q9VNV3|DDX1_DROME ATP-dependent helicase DDX1 (DEAD-box ... 257 2e-68
sp|Q24731|DDX1_DROVI ATP-dependent helicase DDX1 (DEAD box ... 217 2e-56
sp|O02494|IF4A_CRYPV Eukaryotic initiation factor 4A (eIF4A... 79 2e-14
sp|P27639|IF4A_CAEEL Eukaryotic initiation factor 4A (eIF4A... 73 7e-13
sp|P41378|IF4A_WHEAT Eukaryotic initiation factor 4A (eIF4A... 66 1e-10
sp|Q41741|IF4A_MAIZE Eukaryotic initiation factor 4A (eIF4A... 65 1e-10
sp|P87206|IF4A_CANAL Eukaryotic initiation factor 4A (eIF4A... 64 3e-10
>sp|Q92499|DDX1_HUMAN ATP-dependent helicase DDX1 (DEAD-box protein 1) (DEAD-box
protein-retinoblastoma) (DBP-RB)
Length = 740
Score = 273 bits (697), Expect = 4e-73
Identities = 135/236 (57%), Positives = 165/236 (69%)
Frame = +1
Query: 1 GGSNTRDQQQQMATGVDIVVATPGRLQDVIAGRFLSLDNCRFFVLDECDGLLSQGHKALI 180
GG RDQ + GVDIVV TPGRL D+++ L+L RF VLDE DGLLSQG+ I
Sbjct: 325 GGVAARDQLSVLENGVDIVVGTPGRLDDLVSTGKLNLSQVRFLVLDEADGLLSQGYSDFI 384
Query: 181 EGIHRDLPKIGPTGDRLQMIVCSATLHSFEVKKLANSLMHFPQWVDLKGEDSVPDTLHHV 360
+H +P++ G RLQ+IVCSATLHSF+VKKL+ +MHFP WVDLKGEDSVPDT+HHV
Sbjct: 385 NRMHNQIPQVTSDGKRLQVIVCSATLHSFDVKKLSEKIMHFPTWVDLKGEDSVPDTVHHV 444
Query: 361 VCLVDPAADKSWKNRFGGASNSIGSLTDGVHYEDQLNVSVESPEMLSEAVKIIKMKYVME 540
V V+P D+ W+ R G + TD VH +D SPEM SEA+KI+K +Y +
Sbjct: 445 VVPVNPKTDRLWE-RLGKSH----IRTDDVHAKDNTRPGANSPEMWSEAIKILKGEYAVR 499
Query: 541 VIETLKIDRALIFCRTKLDCDNLEQLFITRGGGPTNNKHQFSCVCLHSDRKPDERK 708
I+ K+D+A+IFCRTK+DCDNLEQ FI +GGGP HQFSCVCLH DRKP ERK
Sbjct: 500 AIKEHKMDQAIIFCRTKIDCDNLEQYFIQQGGGPDKKGHQFSCVCLHGDRKPHERK 555
>sp|Q91VR5|DDX1_MOUSE ATP-dependent helicase DDX1 (DEAD-box protein 1)
Length = 740
Score = 273 bits (697), Expect = 4e-73
Identities = 138/236 (58%), Positives = 166/236 (70%)
Frame = +1
Query: 1 GGSNTRDQQQQMATGVDIVVATPGRLQDVIAGRFLSLDNCRFFVLDECDGLLSQGHKALI 180
GG RDQ + GVDIVV TPGRL D+++ L+L RF VLDE DGLLSQG+ I
Sbjct: 325 GGVAARDQLSVLDNGVDIVVGTPGRLDDLVSTGKLNLSQVRFLVLDEADGLLSQGYSDFI 384
Query: 181 EGIHRDLPKIGPTGDRLQMIVCSATLHSFEVKKLANSLMHFPQWVDLKGEDSVPDTLHHV 360
+H +P+I G RLQ+IVCSATLHSF+VKKL+ +MHFP WVDLKGEDSVPDT+HHV
Sbjct: 385 NRMHNQIPQITCDGKRLQVIVCSATLHSFDVKKLSEKIMHFPTWVDLKGEDSVPDTVHHV 444
Query: 361 VCLVDPAADKSWKNRFGGASNSIGSLTDGVHYEDQLNVSVESPEMLSEAVKIIKMKYVME 540
V V+P DK W+ R G N I TD VH +D SPEM SEA+KI+K +Y +
Sbjct: 445 VVPVNPKTDKLWE-RLG--KNHI--RTDDVHAKDNTRPGANSPEMWSEAIKILKGEYAVR 499
Query: 541 VIETLKIDRALIFCRTKLDCDNLEQLFITRGGGPTNNKHQFSCVCLHSDRKPDERK 708
I+ K+D+A+IFCRTK+DCDNLEQ F+ +GGGP HQFSCVCLH DRKP ERK
Sbjct: 500 AIKEHKMDQAIIFCRTKIDCDNLEQYFMQQGGGPDKKGHQFSCVCLHGDRKPHERK 555
>sp|Q641Y8|DDX1_RAT ATP-dependent helicase DDX1 (DEAD-box protein 1)
Length = 740
Score = 272 bits (696), Expect = 6e-73
Identities = 137/236 (58%), Positives = 166/236 (70%)
Frame = +1
Query: 1 GGSNTRDQQQQMATGVDIVVATPGRLQDVIAGRFLSLDNCRFFVLDECDGLLSQGHKALI 180
GG RDQ + GVDIVV TPGRL D+++ L+L RF VLDE DGLLSQG+ I
Sbjct: 325 GGVAARDQLSVLDNGVDIVVGTPGRLDDLVSTGKLNLSQVRFLVLDEADGLLSQGYSDFI 384
Query: 181 EGIHRDLPKIGPTGDRLQMIVCSATLHSFEVKKLANSLMHFPQWVDLKGEDSVPDTLHHV 360
+H +P+I G RLQ+IVCSATLHSF+VKKL+ +MHFP WVDLKGEDSVPDT+HHV
Sbjct: 385 NRMHNQIPQITSDGKRLQVIVCSATLHSFDVKKLSEKIMHFPTWVDLKGEDSVPDTVHHV 444
Query: 361 VCLVDPAADKSWKNRFGGASNSIGSLTDGVHYEDQLNVSVESPEMLSEAVKIIKMKYVME 540
V V+P D+ W+ R G N I TD VH +D SPEM SEA+KI+K +Y +
Sbjct: 445 VVPVNPKTDRLWE-RLG--KNHI--RTDDVHAKDNTRPGANSPEMWSEAIKILKGEYAVR 499
Query: 541 VIETLKIDRALIFCRTKLDCDNLEQLFITRGGGPTNNKHQFSCVCLHSDRKPDERK 708
I+ K+D+A+IFCRTK+DCDNLEQ F+ +GGGP HQFSCVCLH DRKP ERK
Sbjct: 500 AIKEHKMDQAIIFCRTKIDCDNLEQYFMQQGGGPDKKGHQFSCVCLHGDRKPHERK 555
>sp|Q9VNV3|DDX1_DROME ATP-dependent helicase DDX1 (DEAD-box protein 1)
Length = 727
Score = 257 bits (657), Expect = 2e-68
Identities = 130/236 (55%), Positives = 164/236 (69%)
Frame = +1
Query: 1 GGSNTRDQQQQMATGVDIVVATPGRLQDVIAGRFLSLDNCRFFVLDECDGLLSQGHKALI 180
GG +Q+ Q+ G IVV TPGRL+++I + L +CRFFVLDE D LL QG+ LI
Sbjct: 325 GGVRLEEQKAQLMQGTHIVVGTPGRLEEMINSGLVLLTHCRFFVLDEADALLKQGYTELI 384
Query: 181 EGIHRDLPKIGPTGDRLQMIVCSATLHSFEVKKLANSLMHFPQWVDLKGEDSVPDTLHHV 360
+ +H+ +PKI G RLQM+VCSATLH+FEVKK+A LMHFP WVDLKGED+VP+T+HHV
Sbjct: 385 DRLHKQIPKITSDGRRLQMVVCSATLHAFEVKKMAERLMHFPTWVDLKGEDAVPETVHHV 444
Query: 361 VCLVDPAADKSWKNRFGGASNSIGSLTDGVHYEDQLNVSVESPEMLSEAVKIIKMKYVME 540
VCLVDP D +W++ IG TDGVH D ++ S E LS+AVK++K +Y +
Sbjct: 445 VCLVDPQMDTTWQS----LRQPIG--TDGVHDRDNVHPGNHSKETLSQAVKLLKGEYCVH 498
Query: 541 VIETLKIDRALIFCRTKLDCDNLEQLFITRGGGPTNNKHQFSCVCLHSDRKPDERK 708
I+ +DRA+IFCRTK DCDNLE+ RGG KH +SCVCLH DRKP ERK
Sbjct: 499 AIDKHNMDRAIIFCRTKQDCDNLERFLRQRGG-----KH-YSCVCLHGDRKPQERK 548
>sp|Q24731|DDX1_DROVI ATP-dependent helicase DDX1 (DEAD box protein 1)
Length = 400
Score = 217 bits (553), Expect = 2e-56
Identities = 109/191 (57%), Positives = 133/191 (69%)
Frame = +1
Query: 136 DECDGLLSQGHKALIEGIHRDLPKIGPTGDRLQMIVCSATLHSFEVKKLANSLMHFPQWV 315
DE D LL QG+ LIE +H+ +PKI G RLQMIVCSATLH+FEVKK+A LMHFP W
Sbjct: 2 DEADRLLKQGYTDLIERLHKQIPKITSDGCRLQMIVCSATLHAFEVKKMAERLMHFPTWG 61
Query: 316 DLKGEDSVPDTLHHVVCLVDPAADKSWKNRFGGASNSIGSLTDGVHYEDQLNVSVESPEM 495
DLKGED+VP+T+HHVVC+VDP D SW+ TDGVH D ++ + +SPE
Sbjct: 62 DLKGEDAVPETVHHVVCMVDPHTDSSWQQLRQPIH------TDGVHDHDNVHPTNQSPET 115
Query: 496 LSEAVKIIKMKYVMEVIETLKIDRALIFCRTKLDCDNLEQLFITRGGGPTNNKHQFSCVC 675
S+AVK++K +Y + I+ K+DRA+IFCRTK DCDNLEQ RGG +FSCVC
Sbjct: 116 FSQAVKLLKGEYCIRAIDQHKMDRAIIFCRTKQDCDNLEQHLSQRGG------QRFSCVC 169
Query: 676 LHSDRKPDERK 708
LH DRKP ERK
Sbjct: 170 LHGDRKPQERK 180
>sp|O02494|IF4A_CRYPV Eukaryotic initiation factor 4A (eIF4A) (eIF-4A)
Length = 405
Score = 78.6 bits (192), Expect = 2e-14
Identities = 61/236 (25%), Positives = 104/236 (44%)
Frame = +1
Query: 1 GGSNTRDQQQQMATGVDIVVATPGRLQDVIAGRFLSLDNCRFFVLDECDGLLSQGHKALI 180
GG++ RD ++ +GV +VV TPGR+ D++ +L +DN + F+LDE D +LS+G K I
Sbjct: 135 GGTSVRDDMNKLKSGVHMVVGTPGRVFDMLDKGYLRVDNLKLFILDEADEMLSRGFKVQI 194
Query: 181 EGIHRDLPKIGPTGDRLQMIVCSATLHSFEVKKLANSLMHFPQWVDLKGEDSVPDTLHHV 360
I + LP+ +Q+ + SAT+ + E+ L M P+ + +K E+ + +
Sbjct: 195 HDIFKKLPQ------DVQVALFSATMPN-EILHLTTQFMRDPKRILVKQEELTLEGIR-- 245
Query: 361 VCLVDPAADKSWKNRFGGASNSIGSLTDGVHYEDQLNVSVESPEMLSEAVKIIKMKYVME 540
Q V VE E KM +++
Sbjct: 246 ----------------------------------QFYVGVEKDEW--------KMDTLID 263
Query: 541 VIETLKIDRALIFCRTKLDCDNLEQLFITRGGGPTNNKHQFSCVCLHSDRKPDERK 708
+ ETL I +A+I+C T+ D L + + F+C +H D +R+
Sbjct: 264 LYETLTIVQAIIYCNTRRRVDQLTKQM---------RERDFTCSSMHGDMDQKDRE 310
>sp|P27639|IF4A_CAEEL Eukaryotic initiation factor 4A (eIF4A) (eIF-4A)
Length = 402
Score = 73.2 bits (178), Expect = 7e-13
Identities = 58/235 (24%), Positives = 100/235 (42%)
Frame = +1
Query: 1 GGSNTRDQQQQMATGVDIVVATPGRLQDVIAGRFLSLDNCRFFVLDECDGLLSQGHKALI 180
GG++ RD Q+++ G+ +VV TPGR+ D+I L + FVLDE D +LS+G K I
Sbjct: 133 GGTSVRDDQRKLEAGIHVVVGTPGRVGDMINRNALDTSRIKMFVLDEADEMLSRGFKDQI 192
Query: 181 EGIHRDLPKIGPTGDRLQMIVCSATLHSFEVKKLANSLMHFPQWVDLKGEDSVPDTLHHV 360
+ R +P+ +Q+++ SAT+ S EV + N M P + +K ++ + +
Sbjct: 193 YEVFRSMPQ------DVQVVLLSATMPS-EVLDVTNRFMRNPIRILVKKDELTLEGIR-- 243
Query: 361 VCLVDPAADKSWKNRFGGASNSIGSLTDGVHYEDQLNVSVESPEMLSEAVKIIKMKYVME 540
Q ++V+ E K + +
Sbjct: 244 ----------------------------------QFYINVQKDEW--------KFDCLCD 261
Query: 541 VIETLKIDRALIFCRTKLDCDNLEQLFITRGGGPTNNKHQFSCVCLHSDRKPDER 705
+ + + +A+IFC T+ D L + ++QF+ CLH D ER
Sbjct: 262 LYNVVNVTQAVIFCNTRRKVDTLTEKM---------TENQFTVSCLHGDMDQAER 307
>sp|P41378|IF4A_WHEAT Eukaryotic initiation factor 4A (eIF4A) (eIF-4A)
Length = 414
Score = 65.9 bits (159), Expect = 1e-10
Identities = 40/111 (36%), Positives = 66/111 (59%)
Frame = +1
Query: 1 GGSNTRDQQQQMATGVDIVVATPGRLQDVIAGRFLSLDNCRFFVLDECDGLLSQGHKALI 180
GG++ R+ Q+ +A+GV +VV TPGR+ D++ + L DN + FVLDE D +LS+G K I
Sbjct: 145 GGTSVREDQRILASGVHVVVGTPGRVFDIVRRQSLRPDNIKMFVLDEADEMLSRGFKDQI 204
Query: 181 EGIHRDLPKIGPTGDRLQMIVCSATLHSFEVKKLANSLMHFPQWVDLKGED 333
I + LP ++Q+ V SAT+ E ++ M+ P + +K ++
Sbjct: 205 YDIFQLLP------GKIQVGVFSATMPP-EALEITRKFMNKPVRILVKRDE 248
>sp|Q41741|IF4A_MAIZE Eukaryotic initiation factor 4A (eIF4A) (eIF-4A)
Length = 410
Score = 65.5 bits (158), Expect = 1e-10
Identities = 43/133 (32%), Positives = 73/133 (54%)
Frame = +1
Query: 1 GGSNTRDQQQQMATGVDIVVATPGRLQDVIAGRFLSLDNCRFFVLDECDGLLSQGHKALI 180
GG++ R+ Q+ +A+GV +VV TPGR+ D++ + L DN + FVLDE D +LS+G K I
Sbjct: 141 GGTSVREDQRILASGVHVVVGTPGRVFDMLRRQSLRPDNIKMFVLDEADEMLSRGFKDQI 200
Query: 181 EGIHRDLPKIGPTGDRLQMIVCSATLHSFEVKKLANSLMHFPQWVDLKGEDSVPDTLHHV 360
I + LP ++Q+ V SAT+ E ++ M+ P + +K ++ + +
Sbjct: 201 YDIFQLLP------SKIQVGVFSATMPP-EALEITRKFMNKPVRILVKRDELTLEGIKQF 253
Query: 361 VCLVDPAADKSWK 399
+D + WK
Sbjct: 254 YVNID---KEDWK 263
>sp|P87206|IF4A_CANAL Eukaryotic initiation factor 4A (eIF4A) (eIF-4A)
Length = 397
Score = 64.3 bits (155), Expect = 3e-10
Identities = 38/111 (34%), Positives = 61/111 (54%)
Frame = +1
Query: 1 GGSNTRDQQQQMATGVDIVVATPGRLQDVIAGRFLSLDNCRFFVLDECDGLLSQGHKALI 180
GG++ D + +GV IVV TPGR+ D+I R+ D + F+LDE D +LS G K I
Sbjct: 127 GGTSMSDDIEAFRSGVQIVVGTPGRVLDMIERRYFKTDKVKMFILDEADEMLSSGFKEQI 186
Query: 181 EGIHRDLPKIGPTGDRLQMIVCSATLHSFEVKKLANSLMHFPQWVDLKGED 333
I R LP + Q+++ SAT+ +V ++ M+ P + +K ++
Sbjct: 187 YNIFRLLP------ETTQIVLLSATMPQ-DVLEVTTKFMNNPVRILVKKDE 230
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 89,322,937
Number of Sequences: 369166
Number of extensions: 1881315
Number of successful extensions: 8380
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 7381
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8345
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6267895215
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)