Planarian EST Database


Dr_sW_010_M01

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_010_M01
         (782 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8R5C5|ACTY_MOUSE  Beta-centractin (Actin-related protein...   284   2e-76
sp|P42025|ACTY_HUMAN  Beta-centractin (Actin-related protein...   283   5e-76
sp|P61164|ACTZ_MOUSE  Alpha-centractin (Centractin) (Centros...   278   2e-74
sp|P45889|ACTZ_DROME  Actin-like protein 87C                      271   1e-72
sp|P42023|ACTZ_PNECA  Actin-2 (Actin II) (Centractin-like pr...   210   3e-54
sp|P38673|ACTZ_NEUCR  Actin-like protein (Centractin)             195   1e-49
sp|Q8SWN8|ACT_ENCCU  Actin                                        191   3e-48
sp|P27131|ACT1_NAEFO  Actin-1 (Actin I)                           187   2e-47
sp|P02578|ACT1_ACACA  Actin-1                                     187   3e-47
sp|P53496|ACT11_ARATH  Actin-11                                   187   3e-47
>sp|Q8R5C5|ACTY_MOUSE Beta-centractin (Actin-related protein 1B) (ARP1B)
          Length = 376

 Score =  284 bits (726), Expect = 2e-76
 Identities = 135/185 (72%), Positives = 161/185 (87%)
 Frame = +3

Query: 3   VSRYLRLLLRKEGTHFQTSAEFEIVRQLKEKACFLSANPEMQEQSEIDNTTYPLPDGSKL 182
           VSRYLRLLLRKEG  F TSAEFE+VR +KE+AC+LS NP+  E  E +   Y LPDGS L
Sbjct: 190 VSRYLRLLLRKEGADFHTSAEFEVVRTIKERACYLSINPQKDEALETEKVQYTLPDGSTL 249

Query: 183 RIGGAKFRAPELLFRPDLIGSECDGIHEVLAYSIQKSDMDLRQILYGNIVLSGGSTLFKG 362
            +G A+FRAPELLF+PDL+G E +G+HEVLA++I KSDMDLR+ L+ NIVLSGGSTLFKG
Sbjct: 250 DVGPARFRAPELLFQPDLVGDESEGLHEVLAFAIHKSDMDLRRTLFSNIVLSGGSTLFKG 309

Query: 363 FGDRILSELKKLAPKDNKIRISAPQERLYSTWIGGSILASLDTFRRMWISKREFEDDGYR 542
           FGDR+LSE+KKLAPKD KI+ISAPQERLYSTWIGGSILASLDTF++MW+SK+E+E+DG R
Sbjct: 310 FGDRLLSEVKKLAPKDVKIKISAPQERLYSTWIGGSILASLDTFKKMWVSKKEYEEDGSR 369

Query: 543 ALHRK 557
           A+HRK
Sbjct: 370 AIHRK 374
>sp|P42025|ACTY_HUMAN Beta-centractin (Actin-related protein 1B) (ARP1B)
          Length = 376

 Score =  283 bits (723), Expect = 5e-76
 Identities = 134/185 (72%), Positives = 161/185 (87%)
 Frame = +3

Query: 3   VSRYLRLLLRKEGTHFQTSAEFEIVRQLKEKACFLSANPEMQEQSEIDNTTYPLPDGSKL 182
           VSRYLRLLLRKEG  F TSAEFE+VR +KE+AC+LS NP+  E  E +   Y LPDGS L
Sbjct: 190 VSRYLRLLLRKEGVDFHTSAEFEVVRTIKERACYLSINPQKDEALETEKVQYTLPDGSTL 249

Query: 183 RIGGAKFRAPELLFRPDLIGSECDGIHEVLAYSIQKSDMDLRQILYGNIVLSGGSTLFKG 362
            +G A+FRAPELLF+PDL+G E +G+HEV+A++I KSDMDLR+ L+ NIVLSGGSTLFKG
Sbjct: 250 DVGPARFRAPELLFQPDLVGDESEGLHEVVAFAIHKSDMDLRRTLFANIVLSGGSTLFKG 309

Query: 363 FGDRILSELKKLAPKDNKIRISAPQERLYSTWIGGSILASLDTFRRMWISKREFEDDGYR 542
           FGDR+LSE+KKLAPKD KI+ISAPQERLYSTWIGGSILASLDTF++MW+SK+E+E+DG R
Sbjct: 310 FGDRLLSEVKKLAPKDIKIKISAPQERLYSTWIGGSILASLDTFKKMWVSKKEYEEDGSR 369

Query: 543 ALHRK 557
           A+HRK
Sbjct: 370 AIHRK 374
>sp|P61164|ACTZ_MOUSE Alpha-centractin (Centractin) (Centrosome-associated actin homolog)
           (Actin-RPV) (ARP1)
 sp|P61163|ACTZ_HUMAN Alpha-centractin (Centractin) (Centrosome-associated actin homolog)
           (Actin-RPV) (ARP1)
 sp|P61162|ACTZ_CANFA Alpha-centractin (Centractin) (Centrosome-associated actin homolog)
           (Actin-RPV) (ARP1)
          Length = 376

 Score =  278 bits (710), Expect = 2e-74
 Identities = 133/185 (71%), Positives = 160/185 (86%)
 Frame = +3

Query: 3   VSRYLRLLLRKEGTHFQTSAEFEIVRQLKEKACFLSANPEMQEQSEIDNTTYPLPDGSKL 182
           VSR+LRL LRKEG  F +S+EFEIV+ +KE+AC+LS NP+  E  E +   Y LPDGS +
Sbjct: 190 VSRFLRLYLRKEGYDFHSSSEFEIVKAIKERACYLSINPQKDETLETEKAQYYLPDGSTI 249

Query: 183 RIGGAKFRAPELLFRPDLIGSECDGIHEVLAYSIQKSDMDLRQILYGNIVLSGGSTLFKG 362
            IG ++FRAPELLFRPDLIG E +GIHEVL ++IQKSDMDLR+ L+ NIVLSGGSTLFKG
Sbjct: 250 EIGPSRFRAPELLFRPDLIGEESEGIHEVLVFAIQKSDMDLRRTLFSNIVLSGGSTLFKG 309

Query: 363 FGDRILSELKKLAPKDNKIRISAPQERLYSTWIGGSILASLDTFRRMWISKREFEDDGYR 542
           FGDR+LSE+KKLAPKD KIRISAPQERLYSTWIGGSILASLDTF++MW+SK+E+E+DG R
Sbjct: 310 FGDRLLSEVKKLAPKDVKIRISAPQERLYSTWIGGSILASLDTFKKMWVSKKEYEEDGAR 369

Query: 543 ALHRK 557
           ++HRK
Sbjct: 370 SIHRK 374
>sp|P45889|ACTZ_DROME Actin-like protein 87C
          Length = 376

 Score =  271 bits (693), Expect = 1e-72
 Identities = 127/185 (68%), Positives = 160/185 (86%)
 Frame = +3

Query: 3   VSRYLRLLLRKEGTHFQTSAEFEIVRQLKEKACFLSANPEMQEQSEIDNTTYPLPDGSKL 182
           V+RYL+ L+R+EG +F+++AEFEIVR +KEK C+L+ NP+ +E  E +   Y LPDG   
Sbjct: 190 VTRYLKTLIRREGFNFRSTAEFEIVRSIKEKVCYLATNPQKEETVETEKFAYKLPDGKIF 249

Query: 183 RIGGAKFRAPELLFRPDLIGSECDGIHEVLAYSIQKSDMDLRQILYGNIVLSGGSTLFKG 362
            IG A+FRAPE+LFRPDL+G EC+GIH+VL YSI+KSDMDLR++LY NIVLSGGSTLFKG
Sbjct: 250 EIGPARFRAPEVLFRPDLLGEECEGIHDVLMYSIEKSDMDLRKMLYQNIVLSGGSTLFKG 309

Query: 363 FGDRILSELKKLAPKDNKIRISAPQERLYSTWIGGSILASLDTFRRMWISKREFEDDGYR 542
           FGDR+LSELKK + KD KIRI+APQERLYSTW+GGSILASLDTF++MWISKRE+E++G +
Sbjct: 310 FGDRLLSELKKHSAKDLKIRIAAPQERLYSTWMGGSILASLDTFKKMWISKREYEEEGQK 369

Query: 543 ALHRK 557
           A+HRK
Sbjct: 370 AVHRK 374
>sp|P42023|ACTZ_PNECA Actin-2 (Actin II) (Centractin-like protein)
          Length = 385

 Score =  210 bits (535), Expect = 3e-54
 Identities = 108/196 (55%), Positives = 145/196 (73%), Gaps = 11/196 (5%)
 Frame = +3

Query: 3   VSRYLRLLLRKEGTHFQTSAEFEIVRQLKEKACFLSANPEMQEQSEIDNTT--------- 155
           V+ YL+LLLRK GT F TSAE EIVR +KEK  +++ +P  +E+  I+ +          
Sbjct: 189 VTEYLQLLLRKSGTIFHTSAEKEIVRIIKEKCSYVTLDPRKEEKEWINASISGGKDYTKE 248

Query: 156 --YPLPDGSKLRIGGAKFRAPELLFRPDLIGSECDGIHEVLAYSIQKSDMDLRQILYGNI 329
             + LPDG+ LR+G  +FRAPE+LF P++IGSE  GIH+V+  +I + D+DLR+ L+GNI
Sbjct: 249 EEFKLPDGNVLRLGAERFRAPEILFDPEIIGSEYSGIHQVVVDAISRVDLDLRKSLFGNI 308

Query: 330 VLSGGSTLFKGFGDRILSELKKLAPKDNKIRISAPQERLYSTWIGGSILASLDTFRRMWI 509
           VLSGGSTL +GFGDR+LSE+++LA KD KI+I AP ER YSTWIGGSILASL TFR+MW+
Sbjct: 309 VLSGGSTLTRGFGDRLLSEIRRLAVKDVKIKIFAPPERKYSTWIGGSILASLSTFRKMWV 368

Query: 510 SKREFEDDGYRALHRK 557
           S  E+++D    +HRK
Sbjct: 369 SAEEYQEDP-DIIHRK 383
>sp|P38673|ACTZ_NEUCR Actin-like protein (Centractin)
          Length = 380

 Score =  195 bits (495), Expect = 1e-49
 Identities = 98/184 (53%), Positives = 137/184 (74%), Gaps = 7/184 (3%)
 Frame = +3

Query: 3   VSRYLRLLLRKEGTHFQTSAEFEIVRQLKEKACFLSANPEMQEQ----SEIDNTT---YP 161
           V+ YL+ LLRK G  F TSAE E+VR +KE   +++ +P  +E+    +++D      Y 
Sbjct: 188 VTEYLQTLLRKSGYVFHTSAEKEVVRLIKESVTYVAHDPRKEEKEWAAAKMDPAKIAEYV 247

Query: 162 LPDGSKLRIGGAKFRAPELLFRPDLIGSECDGIHEVLAYSIQKSDMDLRQILYGNIVLSG 341
           LPDG+KL+IG  +FRAPE+LF P++IG E  G+H+++  SI ++D+DLR+ LY NIVLSG
Sbjct: 248 LPDGNKLKIGAERFRAPEILFDPEIIGLEYPGVHQIVVDSINRTDLDLRRDLYSNIVLSG 307

Query: 342 GSTLFKGFGDRILSELKKLAPKDNKIRISAPQERLYSTWIGGSILASLDTFRRMWISKRE 521
           GSTL KGFGDR+L+E++KLA KD +I+I AP ER YSTWIGGSILA L TFR+MW+S  +
Sbjct: 308 GSTLTKGFGDRLLTEVQKLAVKDMRIKIFAPPERKYSTWIGGSILAGLSTFRKMWVSIDD 367

Query: 522 FEDD 533
           + ++
Sbjct: 368 WHEN 371
>sp|Q8SWN8|ACT_ENCCU Actin
          Length = 375

 Score =  191 bits (484), Expect = 3e-48
 Identities = 98/186 (52%), Positives = 128/186 (68%), Gaps = 4/186 (2%)
 Frame = +3

Query: 12  YLRLLLRKEGTHFQTSAEFEIVRQLKEKACFLSANPEMQ----EQSEIDNTTYPLPDGSK 179
           YL+L+L + G  F T+AE EIVR +KEK C++S N E      E       TY +PDG  
Sbjct: 188 YLQLILTESGNSFTTTAEREIVRDIKEKLCYVSLNYEEDMRNTEHLASITKTYEMPDGQV 247

Query: 180 LRIGGAKFRAPELLFRPDLIGSECDGIHEVLAYSIQKSDMDLRQILYGNIVLSGGSTLFK 359
           + IG  +FRAPELLF+P L G E  GIH+ +  SI K D+D+R+ LYGNIVLSGG+T++ 
Sbjct: 248 ISIGNERFRAPELLFQPKLRGLELKGIHQNIYDSIMKCDVDIRKELYGNIVLSGGTTMYP 307

Query: 360 GFGDRILSELKKLAPKDNKIRISAPQERLYSTWIGGSILASLDTFRRMWISKREFEDDGY 539
           G  +RIL+E+K LAP   KI + AP ER YS WIGGSILASL TF++MW+SK E+++ G 
Sbjct: 308 GLAERILNEIKALAPPVIKIGVVAPPERKYSVWIGGSILASLSTFQQMWVSKAEYQEHGP 367

Query: 540 RALHRK 557
             +HRK
Sbjct: 368 SIVHRK 373
>sp|P27131|ACT1_NAEFO Actin-1 (Actin I)
          Length = 375

 Score =  187 bits (476), Expect = 2e-47
 Identities = 92/187 (49%), Positives = 129/187 (68%), Gaps = 5/187 (2%)
 Frame = +3

Query: 12  YLRLLLRKEGTHFQTSAEFEIVRQLKEKACFLSANPEMQ-----EQSEIDNTTYPLPDGS 176
           YL  +L + G  F T+AE EIVR +KEK C+++ + E +     E S ++ + Y LPDG+
Sbjct: 188 YLMKILMERGYSFNTTAEREIVRDIKEKLCYIALDFEQEMKIAAESSSVEKS-YELPDGN 246

Query: 177 KLRIGGAKFRAPELLFRPDLIGSECDGIHEVLAYSIQKSDMDLRQILYGNIVLSGGSTLF 356
            + +G  +FR PE+LF+P+ IG E  G+HE    SI K D+D+R+ LYGN+VLSGG+T+F
Sbjct: 247 VITVGNERFRCPEVLFQPNFIGMEAAGVHETTFNSIGKCDIDIRKDLYGNVVLSGGTTMF 306

Query: 357 KGFGDRILSELKKLAPKDNKIRISAPQERLYSTWIGGSILASLDTFRRMWISKREFEDDG 536
           +G  +R+  EL  +AP   KI++ AP ER YS WIGGSILASL TF++MWI+K E+ED G
Sbjct: 307 EGIAERMTKELTNMAPASMKIKVVAPPERKYSVWIGGSILASLSTFQQMWITKEEYEDAG 366

Query: 537 YRALHRK 557
              +HRK
Sbjct: 367 PGIVHRK 373
>sp|P02578|ACT1_ACACA Actin-1
          Length = 375

 Score =  187 bits (475), Expect = 3e-47
 Identities = 94/186 (50%), Positives = 125/186 (67%), Gaps = 4/186 (2%)
 Frame = +3

Query: 12  YLRLLLRKEGTHFQTSAEFEIVRQLKEKACFLSANPEMQEQSEIDNT----TYPLPDGSK 179
           YL  +L + G  F T+AE EIVR +KEK C+++ + E +  +   ++    +Y LPDG  
Sbjct: 188 YLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMHTAASSSALEKSYELPDGQV 247

Query: 180 LRIGGAKFRAPELLFRPDLIGSECDGIHEVLAYSIQKSDMDLRQILYGNIVLSGGSTLFK 359
           + IG  +FRAPE LF+P  +G E  GIHE    SI K D+D+R+ LYGN+VLSGG+T+F 
Sbjct: 248 ITIGNERFRAPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFP 307

Query: 360 GFGDRILSELKKLAPKDNKIRISAPQERLYSTWIGGSILASLDTFRRMWISKREFEDDGY 539
           G  DR+  EL  LAP   KI+I AP ER YS WIGGSILASL TF++MWISK E+++ G 
Sbjct: 308 GIADRMQKELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGP 367

Query: 540 RALHRK 557
             +HRK
Sbjct: 368 SIVHRK 373
>sp|P53496|ACT11_ARATH Actin-11
          Length = 377

 Score =  187 bits (475), Expect = 3e-47
 Identities = 96/188 (51%), Positives = 127/188 (67%), Gaps = 6/188 (3%)
 Frame = +3

Query: 12  YLRLLLRKEGTHFQTSAEFEIVRQLKEKACFLSANPEMQEQSEIDNTT------YPLPDG 173
           YL  +L + G  F TSAE EIVR +KEK  +++ + E  ++ E  NT+      Y LPDG
Sbjct: 190 YLMKILTERGYSFTTSAEREIVRDVKEKLAYIALDYE--QEMETANTSSSVEKSYELPDG 247

Query: 174 SKLRIGGAKFRAPELLFRPDLIGSECDGIHEVLAYSIQKSDMDLRQILYGNIVLSGGSTL 353
             + IGG +FR PE+LF+P L+G E  GIHE    SI K D+D+R+ LYGNIVLSGG+T+
Sbjct: 248 QVITIGGERFRCPEVLFQPSLVGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGTTM 307

Query: 354 FKGFGDRILSELKKLAPKDNKIRISAPQERLYSTWIGGSILASLDTFRRMWISKREFEDD 533
           F G  DR+  E+  LAP   KI++ AP ER YS WIGGSILASL TF++MWI+K E+++ 
Sbjct: 308 FPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDES 367

Query: 534 GYRALHRK 557
           G   +HRK
Sbjct: 368 GPSIVHRK 375
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 99,164,169
Number of Sequences: 369166
Number of extensions: 2143858
Number of successful extensions: 5742
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5220
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5414
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7357347200
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)