Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_010_L22
(794 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P43307|SSRA_HUMAN Translocon-associated protein alpha su... 125 1e-28
sp|P16967|SSRA_CANFA Translocon-associated protein alpha su... 125 1e-28
sp|Q5R4X4|SSRA_PONPY Translocon-associated protein alpha su... 125 1e-28
sp|Q9CY50|SSRA_MOUSE Translocon-associated protein alpha su... 125 2e-28
sp|P53815|SSRA_RABIT Translocon-associated protein alpha su... 124 4e-28
sp|Q7TPJ0|SSRA_RAT Translocon-associated protein alpha subu... 123 5e-28
sp|P45433|SSRA_ONCMY Translocon-associated protein alpha su... 119 9e-27
sp|P45434|SSRA_ARATH Translocon-associated protein alpha su... 43 0.001
sp|P32499|NUP2_YEAST Nucleoporin NUP2 (Nuclear pore protein... 33 1.2
sp|P50898|MIG1_KLULA Regulatory protein MIG1 32 2.6
>sp|P43307|SSRA_HUMAN Translocon-associated protein alpha subunit precursor (TRAP-alpha)
(Signal sequence receptor alpha subunit) (SSR-alpha)
Length = 286
Score = 125 bits (315), Expect = 1e-28
Identities = 70/181 (38%), Positives = 103/181 (56%)
Frame = +1
Query: 154 DDSAAEEAEDKLNEDKIVTEILDSQQDQTEQTFIDGVPVASKNVQAELLFTEPPYNKRSS 333
+DS E+ +D+ ++ E D +D+ E+ + G P AS + +LF +
Sbjct: 43 EDSIIEDEDDEAEVEE--DEPTDLVEDKEEED-VSGEPEASPSADTTILFVK-------- 91
Query: 334 GVGIELPANKPVKMLISFTNTETETNKDYKLVHVEGFLRYPSDYSYYLQNFTARPLDITV 513
G + PAN VK L+ FTN TE D+ + ++ RYP DY +Y+QNFTA PL+ V
Sbjct: 92 --GEDFPANNIVKFLVGFTNKGTE---DFIVESLDASFRYPQDYQFYIQNFTALPLNTVV 146
Query: 514 EPKREATVYYSFTPAQQLAGRPFGLTLRIAYHNNDGKMFTSTFFNDTVQVIEIEEGFDTE 693
P+R+AT YSF PA+ + GRPFGL + + Y + +G +F FN TV VIE E+G D E
Sbjct: 147 PPQRQATFEYSFIPAEPMGGRPFGLVINLNYKDLNGNVFQDAVFNQTVTVIEREDGLDGE 206
Query: 694 S 696
+
Sbjct: 207 T 207
>sp|P16967|SSRA_CANFA Translocon-associated protein alpha subunit precursor (TRAP-alpha)
(Signal sequence receptor alpha subunit) (SSR-alpha)
(PGP35)
Length = 286
Score = 125 bits (315), Expect = 1e-28
Identities = 69/181 (38%), Positives = 103/181 (56%)
Frame = +1
Query: 154 DDSAAEEAEDKLNEDKIVTEILDSQQDQTEQTFIDGVPVASKNVQAELLFTEPPYNKRSS 333
+DS E+ +D+ ++ E D +D+ E+ + G P AS + +LF +
Sbjct: 43 EDSIIEDEDDEAEVEE--DEPTDLAEDKEEED-VSGEPEASPSADTTILFVK-------- 91
Query: 334 GVGIELPANKPVKMLISFTNTETETNKDYKLVHVEGFLRYPSDYSYYLQNFTARPLDITV 513
G + PAN VK L+ FTN TE D+ + ++ RYP DY +Y+QNFTA PL+ V
Sbjct: 92 --GEDFPANNIVKFLVGFTNKGTE---DFIVESLDASFRYPQDYQFYIQNFTALPLNTVV 146
Query: 514 EPKREATVYYSFTPAQQLAGRPFGLTLRIAYHNNDGKMFTSTFFNDTVQVIEIEEGFDTE 693
P+R+AT YSF PA+ + GRPFGL + + Y + +G +F FN TV +IE E+G D E
Sbjct: 147 PPQRQATFEYSFIPAEPMGGRPFGLVINLNYKDLNGNVFQDAVFNQTVTIIEREDGLDGE 206
Query: 694 S 696
+
Sbjct: 207 T 207
>sp|Q5R4X4|SSRA_PONPY Translocon-associated protein alpha subunit precursor (TRAP-alpha)
(Signal sequence receptor alpha subunit) (SSR-alpha)
Length = 291
Score = 125 bits (315), Expect = 1e-28
Identities = 70/181 (38%), Positives = 103/181 (56%)
Frame = +1
Query: 154 DDSAAEEAEDKLNEDKIVTEILDSQQDQTEQTFIDGVPVASKNVQAELLFTEPPYNKRSS 333
+DS E+ +D+ ++ E D +D+ E+ + G P AS + +LF +
Sbjct: 43 EDSIIEDEDDEAEVEE--DEPTDLVEDKEEED-VSGEPEASPSADTTILFVK-------- 91
Query: 334 GVGIELPANKPVKMLISFTNTETETNKDYKLVHVEGFLRYPSDYSYYLQNFTARPLDITV 513
G + PAN VK L+ FTN TE D+ + ++ RYP DY +Y+QNFTA PL+ V
Sbjct: 92 --GEDFPANNIVKFLVGFTNKGTE---DFIVESLDASFRYPQDYQFYIQNFTALPLNTVV 146
Query: 514 EPKREATVYYSFTPAQQLAGRPFGLTLRIAYHNNDGKMFTSTFFNDTVQVIEIEEGFDTE 693
P+R+AT YSF PA+ + GRPFGL + + Y + +G +F FN TV VIE E+G D E
Sbjct: 147 PPQRQATFEYSFIPAEPMGGRPFGLVINLNYKDLNGNVFQDAVFNQTVTVIEREDGLDGE 206
Query: 694 S 696
+
Sbjct: 207 T 207
>sp|Q9CY50|SSRA_MOUSE Translocon-associated protein alpha subunit precursor (TRAP-alpha)
(Signal sequence receptor alpha subunit) (SSR-alpha)
Length = 286
Score = 125 bits (313), Expect = 2e-28
Identities = 69/183 (37%), Positives = 103/183 (56%)
Frame = +1
Query: 148 VTDDSAAEEAEDKLNEDKIVTEILDSQQDQTEQTFIDGVPVASKNVQAELLFTEPPYNKR 327
+ +DS E+ +D+ ++ E D +D+ E+ + P AS + +LF +
Sbjct: 41 IVEDSIIEDEDDEAEVEE--DEPTDLAEDKEEED-VSSEPEASPSADTTILFVK------ 91
Query: 328 SSGVGIELPANKPVKMLISFTNTETETNKDYKLVHVEGFLRYPSDYSYYLQNFTARPLDI 507
G + PAN VK L+ FTN TE D+ + ++ RYP DY +Y+QNFTA PL+
Sbjct: 92 ----GEDFPANNIVKFLVGFTNKGTE---DFIVESLDASFRYPQDYQFYIQNFTALPLNT 144
Query: 508 TVEPKREATVYYSFTPAQQLAGRPFGLTLRIAYHNNDGKMFTSTFFNDTVQVIEIEEGFD 687
V P+R+AT YSF PA+ + GRPFGL + + Y + +G +F FN TV VIE E+G D
Sbjct: 145 VVPPQRQATFEYSFIPAEPMGGRPFGLVINLNYKDLNGNVFQDAVFNQTVTVIEREDGLD 204
Query: 688 TES 696
E+
Sbjct: 205 GET 207
>sp|P53815|SSRA_RABIT Translocon-associated protein alpha subunit precursor (TRAP-alpha)
(Signal sequence receptor alpha subunit) (SSR-alpha)
Length = 286
Score = 124 bits (310), Expect = 4e-28
Identities = 68/181 (37%), Positives = 103/181 (56%)
Frame = +1
Query: 154 DDSAAEEAEDKLNEDKIVTEILDSQQDQTEQTFIDGVPVASKNVQAELLFTEPPYNKRSS 333
+DS E+ +D+ ++ E D +D+ E+ + G P AS + +LF +
Sbjct: 43 EDSVIEDEDDEAEVEE--DEPTDLAEDREEED-VSGEPEASPSADTTILFVK-------- 91
Query: 334 GVGIELPANKPVKMLISFTNTETETNKDYKLVHVEGFLRYPSDYSYYLQNFTARPLDITV 513
G + PAN V+ L+ FTN TE D+ + ++ RYP DY +Y+QNFTA PL+ V
Sbjct: 92 --GEDFPANNIVRFLVGFTNKGTE---DFIVESLDASFRYPQDYQFYIQNFTALPLNTIV 146
Query: 514 EPKREATVYYSFTPAQQLAGRPFGLTLRIAYHNNDGKMFTSTFFNDTVQVIEIEEGFDTE 693
P+R+AT YSF PA+ + GRPFGL + + Y + +G +F FN TV VIE E+G D +
Sbjct: 147 PPQRQATFEYSFIPAEPMGGRPFGLVINLNYKDFNGNVFQDAVFNQTVTVIEREDGLDGQ 206
Query: 694 S 696
+
Sbjct: 207 T 207
>sp|Q7TPJ0|SSRA_RAT Translocon-associated protein alpha subunit precursor (TRAP-alpha)
(Signal sequence receptor alpha subunit) (SSR-alpha)
(Liver regeneration-related protein LRRG137)
Length = 319
Score = 123 bits (309), Expect = 5e-28
Identities = 68/176 (38%), Positives = 99/176 (56%)
Frame = +1
Query: 169 EEAEDKLNEDKIVTEILDSQQDQTEQTFIDGVPVASKNVQAELLFTEPPYNKRSSGVGIE 348
E+ E ++ ED E D +++ E+ + P AS + +LF + G +
Sbjct: 50 EDDEAEVEED----EPTDLAEEKEEEEDVSSEPEASPSADTTILFVK----------GED 95
Query: 349 LPANKPVKMLISFTNTETETNKDYKLVHVEGFLRYPSDYSYYLQNFTARPLDITVEPKRE 528
PAN VK L+ FTN TE D+ + ++ RYP DY +Y+QNFTA PL+ V P+R+
Sbjct: 96 FPANNIVKFLVGFTNKGTE---DFIVESLDASFRYPQDYQFYIQNFTALPLNTIVPPQRQ 152
Query: 529 ATVYYSFTPAQQLAGRPFGLTLRIAYHNNDGKMFTSTFFNDTVQVIEIEEGFDTES 696
AT YSF PA+ + GRPFGL + + Y + +G +F FN TV VIE E+G D E+
Sbjct: 153 ATFEYSFIPAEPMGGRPFGLVINLNYKDLNGNVFQDAVFNQTVTVIEREDGLDGET 208
>sp|P45433|SSRA_ONCMY Translocon-associated protein alpha subunit precursor (TRAP-alpha)
(Signal sequence receptor alpha subunit) (SSR-alpha)
Length = 288
Score = 119 bits (298), Expect = 9e-27
Identities = 62/183 (33%), Positives = 103/183 (56%), Gaps = 3/183 (1%)
Frame = +1
Query: 157 DSAAEEAEDKLNEDKIVTEIL---DSQQDQTEQTFIDGVPVASKNVQAELLFTEPPYNKR 327
DS +E E++ ++ +V E +D ++ G + + ++F
Sbjct: 39 DSTVDEEEEEEEDEVLVEEDQVPGSETEDDIDEDAAVGDVTSHPDADTTIVFV------- 91
Query: 328 SSGVGIELPANKPVKMLISFTNTETETNKDYKLVHVEGFLRYPSDYSYYLQNFTARPLDI 507
G E PAN+ VK L+ FTN + ++D+ + +E RYP D+ +Y+QNFTA PL
Sbjct: 92 ---TGEEFPANEIVKFLVGFTN---KGSQDFTVHSLEASFRYPQDFQFYIQNFTALPLST 145
Query: 508 TVEPKREATVYYSFTPAQQLAGRPFGLTLRIAYHNNDGKMFTSTFFNDTVQVIEIEEGFD 687
V+P+++A+ YSF PAQ +AGRPFGL + + Y +++G F + +N TV ++E+EEG D
Sbjct: 146 VVQPQKQASFEYSFIPAQPMAGRPFGLVILLNYQDSEGNGFQTAIYNQTVTIVELEEGLD 205
Query: 688 TES 696
E+
Sbjct: 206 GET 208
>sp|P45434|SSRA_ARATH Translocon-associated protein alpha subunit precursor (TRAP-alpha)
(Signal sequence receptor alpha subunit) (SSR-alpha)
Length = 258
Score = 42.7 bits (99), Expect = 0.001
Identities = 44/177 (24%), Positives = 74/177 (41%), Gaps = 3/177 (1%)
Frame = +1
Query: 148 VTDDSAAEEAEDKLNEDKIVTEILDSQQDQTEQTF--IDGVPVASKNVQAELLFTEPPYN 321
+ DD E +D + ED D D +F ++ V V KN A+L+
Sbjct: 34 LVDDVVGENTDDAVEED-------DHDLDMNLSSFPGVETVCVFPKN-SAKLV------- 78
Query: 322 KRSSGVGIELPANKPVKMLISFTNTETETNKDYKLVHVEGFLRYPSDYSYYLQNFTARPL 501
PA + ++L+ N E ++ + + P D+ +QN T L
Sbjct: 79 ----------PAGEETELLVGLKN---EGKTRVGVMGIRASVHLPYDHKLLVQNLTMLRL 125
Query: 502 D-ITVEPKREATVYYSFTPAQQLAGRPFGLTLRIAYHNNDGKMFTSTFFNDTVQVIE 669
+ ++ +AT Y F +Q L F L I Y + +GK + S F+N T++V+E
Sbjct: 126 NNASIPTSLQATFPYIFAVSQYLQPGAFDLVGYIIY-DVEGKPYQSVFYNGTIEVVE 181
>sp|P32499|NUP2_YEAST Nucleoporin NUP2 (Nuclear pore protein NUP2) (p95)
Length = 720
Score = 32.7 bits (73), Expect = 1.2
Identities = 30/102 (29%), Positives = 51/102 (50%), Gaps = 11/102 (10%)
Frame = +1
Query: 151 TDDSAAEEAEDKLNEDKIVTEILDSQQ--DQTEQTFIDGVPVASKNVQAELLFT---EPP 315
T +S E++ D + + + + S+ D ++ +F GVP +SKN ++ +F+ P
Sbjct: 334 TSNSKPEKSSDSNDSNPSFSFSIPSKNTPDASKPSFSFGVPNSSKNETSKPVFSFGAATP 393
Query: 316 YNKRSS----GVGIELPANKPVKMLISFTNT--ETETNKDYK 423
K +S +E P++KP LIS T E E+ KD K
Sbjct: 394 SAKEASQEDDNNNVEKPSSKPAFNLISNAGTEKEKESKKDSK 435
>sp|P50898|MIG1_KLULA Regulatory protein MIG1
Length = 474
Score = 31.6 bits (70), Expect = 2.6
Identities = 21/80 (26%), Positives = 36/80 (45%), Gaps = 8/80 (10%)
Frame = +1
Query: 220 DSQQDQTEQTFIDGVPVASKNVQAELLFTEPPYNKRSSGVGIELPANKPVKMLIS----- 384
+S + + FID +P+ S + L FT N SSG+ + PA++ +S
Sbjct: 199 ESDKASSSFKFIDKLPLTSSSSSPSLSFTSHSINNSSSGLLLPRPASRAKLSALSSLQRM 258
Query: 385 ---FTNTETETNKDYKLVHV 435
N+E+ + LVH+
Sbjct: 259 TPLSQNSESYNHSQQNLVHL 278
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 73,417,899
Number of Sequences: 369166
Number of extensions: 1262235
Number of successful extensions: 3156
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3079
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3145
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7473924075
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)