Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_010_L20
(813 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q13686|ALKBH_HUMAN Alkylated DNA repair protein alkB hom... 121 2e-27
sp|Q9SA98|ALKBH_ARATH Alkylated DNA repair protein alkB hom... 99 1e-20
sp|O60066|ALKBH_SCHPO Alkylated DNA repair protein alkB hom... 97 5e-20
sp|P37462|ALKB_SALTY Alkylated DNA repair protein alkB 67 8e-11
sp|P05050|ALKB_ECOLI Alkylated DNA repair protein alkB 64 4e-10
sp|O05725|ALKB_CAUCR Alkylated DNA repair protein alkB homolog 62 2e-09
sp|P57210|SYM_BUCAI Methionyl-tRNA synthetase (Methionine--... 33 0.93
sp|P90682|CATA_ASCSU Catalase 31 4.6
sp|P54308|TERL_BPSPP Terminase large subunit (G2P) 30 6.0
>sp|Q13686|ALKBH_HUMAN Alkylated DNA repair protein alkB homolog
Length = 389
Score = 121 bits (304), Expect = 2e-27
Identities = 66/204 (32%), Positives = 108/204 (52%), Gaps = 5/204 (2%)
Frame = +2
Query: 176 AYEIGENSGFYYIPNPFNGDILMQLIVDSLMKWPTTINRMNLD--IEQDRPNDIWKATIE 349
AY + GF +IPNPF + L + N NLD + ++ D+W+ + E
Sbjct: 90 AYGLKGYPGFIFIPNPFLPGYQWHWVKQCLKLYSQKPNVCNLDKHMSKEETQDLWEQSKE 149
Query: 350 ELGN---CRKLSETWLYKLRWTSMGYHYQWTDRIYKKNCRSNIPDNLSVVMKDIISLLNW 520
L ++ + L KLRW ++GYHY W + Y + + P +L + + + + +
Sbjct: 150 FLRYKEATKRRPRSLLEKLRWVTVGYHYNWDSKKYSADHYTPFPSDLGFLSEQVAAACGF 209
Query: 521 KEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSMSLGQPAVYLLGTSDPLSVPIPIN 700
++F EA I+NYY L+S + H D +E PL+S S GQ A++LLG P +
Sbjct: 210 EDFRAEAGILNYYRLDSTLGIHVDRSELDHSKPLLSFSFGQSAIFLLGGLQRDEAPTAMF 269
Query: 701 LNHGDVVVMSGKSRLAYHAVPKII 772
++ GD+++MSG SRL HAVP+++
Sbjct: 270 MHSGDIMIMSGFSRLLNHAVPRVL 293
>sp|Q9SA98|ALKBH_ARATH Alkylated DNA repair protein alkB homolog
Length = 354
Score = 99.4 bits (246), Expect = 1e-20
Identities = 88/298 (29%), Positives = 138/298 (46%), Gaps = 41/298 (13%)
Frame = +2
Query: 14 YEPESLLKPHYKKYKNRSYTLDELTEFRKFSNPIQLNIKNCKLLNSKAFKNEIVA---YE 184
YE +S K KK + L EL +F N I N N +L +++ + +
Sbjct: 27 YEQDS--KFSRKKKLPKPIDLSELLDF----NLISQNFNNDGVLPDGIRVSKVDSSPVFC 80
Query: 185 IGENSGFYYIPNPFNGDILMQLIVDSLMKWPTTINRMNLD-------------------I 307
I GFY+IP+ + + I +SL +P NR N + +
Sbjct: 81 IDNRPGFYFIPDALSLKEQCKWIKESLTSFPQPPNRTNHNAIYGPIDDLFDSAKENKVLV 140
Query: 308 EQDRPNDIWK----ATIEEL--GNCRKLSETWLY-KLRWTSMGYHYQWTDRIYKKNC-RS 463
+ D N+ WK IE+ +C+ +S + L KLRW+++G + W+ R Y + +
Sbjct: 141 QDDLTNNKWKFYEEVDIEKATRSSCKSVSASVLLRKLRWSTLGLQFDWSKRNYDVSLPHN 200
Query: 464 NIPDNLSVVMKD--IISLLNWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSMSL 637
NIPD L + K I++ + +EF PE IVNY+ + + H D E P+VSMSL
Sbjct: 201 NIPDALCQLAKTHAAIAMPDGEEFRPEGAIVNYFGIGDTLGGHLDDMEADWSKPIVSMSL 260
Query: 638 GQPAVYLLGTSDPLSVPIPINLNHGDVVVMSGKSRLAYH---------AVPKIIKIEE 784
G A++LLG P + L GDVV+M+G++R +H +P+I EE
Sbjct: 261 GCKAIFLLGGKSKDDPPHAMYLRSGDVVLMAGEARECFHGNLLHFQLDGIPRIFTGEE 318
>sp|O60066|ALKBH_SCHPO Alkylated DNA repair protein alkB homolog
Length = 273
Score = 97.1 bits (240), Expect = 5e-20
Identities = 71/265 (26%), Positives = 125/265 (47%), Gaps = 19/265 (7%)
Frame = +2
Query: 17 EPESLLKPHYKKYKNRSYTLDELTEFRKFSNPIQLNIKNCKLLNSKAFKNEIVAYEIGEN 196
E ++ + K+YK R+ T+ +++E ++P + + + F + +
Sbjct: 2 EQANVFRLEEKRYKCRADTIPDMSEVLDPNDPQSFGFEALVEIKPRVFSFQ-------KA 54
Query: 197 SGFYYIPNPFNGDILMQLIVDSLM-KWPTTINRMNLDIEQDRP---NDIWK--------A 340
G + N + ++ MQL+ + + N+ NL P + IW+ +
Sbjct: 55 PGLLILKNYVSSELQMQLLKSIMFTQIQDPENKTNLSPFYQLPLGNDSIWRRYYNGDGES 114
Query: 341 TIEELGNCRKLSETWLY--KLRWTSMGYHYQWTDRIYKKNCRS-----NIPDNLSVVMKD 499
I+ LG + L+ L KLRW ++G Y WT + Y +S ++ D + V+K+
Sbjct: 115 IIDGLGETKPLTVDRLVHKKLRWVTLGEQYDWTTKEYPDPSKSPGFPKDLGDFVEKVVKE 174
Query: 500 IISLLNWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSMSLGQPAVYLLGTSDPL 679
L+WK EA IVN+Y + H D +E +PL+S+S+G +YL+GT
Sbjct: 175 STDFLHWKA---EAAIVNFYSPGDTLSAHIDESEEDLTLPLISLSMGLDCIYLIGTESRS 231
Query: 680 SVPIPINLNHGDVVVMSGKSRLAYH 754
P + L+ GDVV+M+G SR A+H
Sbjct: 232 EKPSALRLHSGDVVIMTGTSRKAFH 256
>sp|P37462|ALKB_SALTY Alkylated DNA repair protein alkB
Length = 216
Score = 66.6 bits (161), Expect = 8e-11
Identities = 45/127 (35%), Positives = 65/127 (51%), Gaps = 5/127 (3%)
Frame = +2
Query: 395 LRWTS--MGYHYQWTDRIYKKNCRSNIPDNLSVVMKDIISLLNWKEFIPEATIVNYYHLN 568
L WT+ GY Y D + K + +P + + V + + F P+A ++N Y
Sbjct: 67 LGWTTDRHGYCYAVRDPLTDKPWPA-LPLSFASVCRQAAIAAGYASFQPDACLINRYAPG 125
Query: 569 SAMCFHQDHAEFAKDVPLVSMSLGQPAVYLLG---TSDPLSVPIPINLNHGDVVVMSGKS 739
+ + HQD E P+VS+SLG PAV+ G SDP+ I L HGD+VV G+S
Sbjct: 126 AKLSLHQDKDEPDLRAPIVSVSLGVPAVFQFGGLRRSDPIQ---RILLEHGDIVVWGGES 182
Query: 740 RLAYHAV 760
RL YH +
Sbjct: 183 RLFYHGI 189
>sp|P05050|ALKB_ECOLI Alkylated DNA repair protein alkB
Length = 216
Score = 64.3 bits (155), Expect = 4e-10
Identities = 43/127 (33%), Positives = 65/127 (51%), Gaps = 5/127 (3%)
Frame = +2
Query: 395 LRWTS--MGYHYQWTDRIYKKNCRSNIPDNLSVVMKDIISLLNWKEFIPEATIVNYYHLN 568
L WT+ GY Y D K + +P + + + + + +F P+A ++N Y
Sbjct: 67 LGWTTHRQGYLYSPIDPQTNKPWPA-MPQSFHNLCQRAATAAGYPDFQPDACLINRYAPG 125
Query: 569 SAMCFHQDHAEFAKDVPLVSMSLGQPAVYLLG---TSDPLSVPIPINLNHGDVVVMSGKS 739
+ + HQD E P+VS+SLG PA++ G +DPL + L HGDVVV G+S
Sbjct: 126 AKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLKRNDPLK---RLLLEHGDVVVWGGES 182
Query: 740 RLAYHAV 760
RL YH +
Sbjct: 183 RLFYHGI 189
>sp|O05725|ALKB_CAUCR Alkylated DNA repair protein alkB homolog
Length = 220
Score = 61.6 bits (148), Expect = 2e-09
Identities = 43/139 (30%), Positives = 68/139 (48%)
Frame = +2
Query: 356 GNCRKLSETWLYKLRWTSMGYHYQWTDRIYKKNCRSNIPDNLSVVMKDIISLLNWKEFIP 535
G ++ T L L WTS Y++ DR PD + + D+ ++L E P
Sbjct: 62 GKPMSVAMTALGSLGWTSDARGYRYVDR--HPETGRPWPD-MPPALLDLWTVLGDPETPP 118
Query: 536 EATIVNYYHLNSAMCFHQDHAEFAKDVPLVSMSLGQPAVYLLGTSDPLSVPIPINLNHGD 715
++ +VN Y + M HQD E P++S+SLG AV+ +G + + L GD
Sbjct: 119 DSCLVNLYRDGARMGLHQDRDEADPRFPVLSISLGDTAVFRIGGVNRKDPTRSLRLASGD 178
Query: 716 VVVMSGKSRLAYHAVPKII 772
V + G +RLA+H V +I+
Sbjct: 179 VCRLLGPARLAFHGVDRIL 197
>sp|P57210|SYM_BUCAI Methionyl-tRNA synthetase (Methionine--tRNA ligase) (MetRS)
Length = 547
Score = 33.1 bits (74), Expect = 0.93
Identities = 14/50 (28%), Positives = 26/50 (52%)
Frame = -2
Query: 503 LCLS*QQINYQEYLSDNSFYKFYLSIGNDIPWMSIVIYTTMFPIISYNFP 354
LC +++N+ E + NS Y+ Y IG DI + + + + +S+ P
Sbjct: 268 LCFKSKKLNFNELWNQNSNYELYHFIGKDIIYFHTLFWPAILEAVSFRQP 317
>sp|P90682|CATA_ASCSU Catalase
Length = 541
Score = 30.8 bits (68), Expect = 4.6
Identities = 27/99 (27%), Positives = 42/99 (42%), Gaps = 5/99 (5%)
Frame = +2
Query: 86 TEFRKFS-NPIQLNIKNCKLLNSKAFKNEIVAYEIGENSGFY--YIPNPFNGDILMQLIV 256
T+F + N +QL I NC + + + N G Y PN FNG I +
Sbjct: 360 TQFHRLGPNYVQLPI-NCPYRSRAHNTQRDGCFALDYNQGGMPTYHPNSFNGAIERTDVK 418
Query: 257 DSLMKWPTTINRMNLDIEQD--RPNDIWKATIEELGNCR 367
+S ++R N D E + +P D+W ++E R
Sbjct: 419 ESAWSVSGDVDRFNGDDEDNFSQPRDLWLKVMDETERAR 457
>sp|P54308|TERL_BPSPP Terminase large subunit (G2P)
Length = 422
Score = 30.4 bits (67), Expect = 6.0
Identities = 12/29 (41%), Positives = 19/29 (65%)
Frame = +2
Query: 11 GYEPESLLKPHYKKYKNRSYTLDELTEFR 97
G +P + ++ HY K KNR Y +DEL + +
Sbjct: 270 GPDPLAFVRWHYDKRKNRIYAIDELVDHK 298
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 90,498,100
Number of Sequences: 369166
Number of extensions: 1853003
Number of successful extensions: 4934
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 4797
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4927
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7763237265
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)