Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_010_L16
(820 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P39656|OST48_HUMAN Dolichyl-diphosphooligosaccharide--pr... 291 2e-78
sp|O54734|OST48_MOUSE Dolichyl-diphosphooligosaccharide--pr... 288 1e-77
sp|Q05052|OST48_CANFA Dolichyl-diphosphooligosaccharide--pr... 286 4e-77
sp|P48440|OST48_CHICK Dolichyl-diphosphooligosaccharide--pr... 285 1e-76
sp|Q24319|OST48_DROME Dolichyl-diphosphooligosaccharide--pr... 284 2e-76
sp|P45971|OST48_CAEEL Probable dolichyl-diphosphooligosacch... 209 1e-53
sp|Q29381|OST48_PIG Dolichyl-diphosphooligosaccharide--prot... 123 7e-28
sp|P33767|OSTB_YEAST Dolichyl-diphosphooligosaccharide--pro... 49 2e-05
sp|Q01811|VNSS_INSVN Nonstructural protein NS-S 35 0.19
sp|Q6KHS4|RS7_MYCMO 30S ribosomal protein S7 35 0.19
>sp|P39656|OST48_HUMAN Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit) (DDOST 48 kDa subunit)
Length = 439
Score = 291 bits (744), Expect = 2e-78
Identities = 143/241 (59%), Positives = 188/241 (78%), Gaps = 2/241 (0%)
Frame = +2
Query: 104 TLVLLETFATKETHSIFFKSLRERGHKLTYHLADDSSLILNKFGEYLYKNVIIFAPSVRE 283
TLVLL+ +ETHS+FF+SL++RG +LT+ ADD SL L K+GE+LY N+IIF+PSV +
Sbjct: 30 TLVLLDNLNVRETHSLFFRSLKDRGFELTFKTADDPSLSLIKYGEFLYDNLIIFSPSVED 89
Query: 284 FGGKIDVPTLMNFVDEGGNILVAASSKIGEAIRELGSECGIEFDEEKTAVIDHHNFDRKD 463
FGG I+V T+ F+D GG++LVAASS IG+ +RELGSECGIEFDEEKTAVIDHHN+D D
Sbjct: 90 FGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEEKTAVIDHHNYDISD 149
Query: 464 DGKHTLIIADEANVAKIPIIVGKVD-SPVLFKGVGIAADDQNPLLINLLHASSSAYSFNP 640
G+HTLI+AD N+ K P IVGK +P+LF+GVG+ AD NPL++++L SS++YSF P
Sbjct: 150 LGQHTLIVADTENLLKAPTIVGKSSLNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFP 209
Query: 641 DKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDDLFTASVEHAI-SKQTHARS 817
DKP+ YP++VG NT LI LQARNNARV+F GSLD FSD F ++V+ A Q ++++
Sbjct: 210 DKPITQYPHAVGKNTLLIAGLQARNNARVIFSGSLDFFSDSFFNSAVQKAAPGSQRYSQT 269
Query: 818 G 820
G
Sbjct: 270 G 270
>sp|O54734|OST48_MOUSE Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit) (DDOST 48 kDa subunit)
Length = 441
Score = 288 bits (738), Expect = 1e-77
Identities = 142/241 (58%), Positives = 188/241 (78%), Gaps = 2/241 (0%)
Frame = +2
Query: 104 TLVLLETFATKETHSIFFKSLRERGHKLTYHLADDSSLILNKFGEYLYKNVIIFAPSVRE 283
TLVLL+ ++THS+FF+SL++RG +LT+ ADD SL L K+GE+LY N+IIF+PSV +
Sbjct: 32 TLVLLDNLNVRDTHSLFFRSLKDRGFELTFKTADDPSLSLIKYGEFLYDNLIIFSPSVED 91
Query: 284 FGGKIDVPTLMNFVDEGGNILVAASSKIGEAIRELGSECGIEFDEEKTAVIDHHNFDRKD 463
FGG I+V T+ F+D GG++LVAASS IG+ +RELGSECGIEFDEEKTAVIDHHN+D D
Sbjct: 92 FGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEEKTAVIDHHNYDVSD 151
Query: 464 DGKHTLIIADEANVAKIPIIVGKVD-SPVLFKGVGIAADDQNPLLINLLHASSSAYSFNP 640
G+HTLI+AD N+ K P IVGK +P+LF+GVG+ AD NPL++++L SS++YSF P
Sbjct: 152 LGQHTLIVADTENLLKAPTIVGKSSLNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFP 211
Query: 641 DKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDDLFTASVEHAI-SKQTHARS 817
DKP+ YP++VG NT LI LQARNNARV+F GSLD FSD F ++V+ A Q ++++
Sbjct: 212 DKPITQYPHAVGRNTLLIAGLQARNNARVIFSGSLDFFSDAFFNSAVQKATPGAQRYSQT 271
Query: 818 G 820
G
Sbjct: 272 G 272
>sp|Q05052|OST48_CANFA Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit)
Length = 445
Score = 286 bits (733), Expect = 4e-77
Identities = 141/241 (58%), Positives = 187/241 (77%), Gaps = 2/241 (0%)
Frame = +2
Query: 104 TLVLLETFATKETHSIFFKSLRERGHKLTYHLADDSSLILNKFGEYLYKNVIIFAPSVRE 283
TLVLL+ +ETHS+FF+SL++R +LT+ ADD SL L K+GE+LY N+IIF+PSV +
Sbjct: 36 TLVLLDNLNLRETHSLFFRSLKDRAFELTFKTADDPSLSLIKYGEFLYDNLIIFSPSVED 95
Query: 284 FGGKIDVPTLMNFVDEGGNILVAASSKIGEAIRELGSECGIEFDEEKTAVIDHHNFDRKD 463
FGG I+V T+ F+D GG++LVAASS IG+ +RELGSECGIEFDEEKTAVIDHHN+D D
Sbjct: 96 FGGNINVETISTFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEEKTAVIDHHNYDVSD 155
Query: 464 DGKHTLIIADEANVAKIPIIVGKVD-SPVLFKGVGIAADDQNPLLINLLHASSSAYSFNP 640
G+HTLI+AD N+ K P IVG+ +P+LF+GVG+ AD NPL++++L SS++YSF P
Sbjct: 156 LGQHTLIVADPENLLKAPTIVGRSSLNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFP 215
Query: 641 DKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDDLFTASVEHAI-SKQTHARS 817
DKP+ YP++VG NT LI LQARNNARV+F GSLD FSD F ++V+ A Q ++++
Sbjct: 216 DKPITQYPHAVGKNTLLIAGLQARNNARVVFSGSLDFFSDAFFNSAVQKAAPGSQRYSQT 275
Query: 818 G 820
G
Sbjct: 276 G 276
>sp|P48440|OST48_CHICK Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit (Oligosaccharyl transferase 48 kDa subunit)
(OST 50 kDa subunit) (DDOST 48 kDa subunit)
Length = 413
Score = 285 bits (728), Expect = 1e-76
Identities = 137/230 (59%), Positives = 182/230 (79%), Gaps = 1/230 (0%)
Frame = +2
Query: 104 TLVLLETFATKETHSIFFKSLRERGHKLTYHLADDSSLILNKFGEYLYKNVIIFAPSVRE 283
+LVLLE ++THS+FF+SL +RG +LT+ ADD+ L L K+GE+LY N+IIF+PS+ +
Sbjct: 4 SLVLLENLNLRDTHSLFFRSLADRGFELTFRTADDAGLSLIKYGEFLYDNLIIFSPSIED 63
Query: 284 FGGKIDVPTLMNFVDEGGNILVAASSKIGEAIRELGSECGIEFDEEKTAVIDHHNFDRKD 463
FGG I+V T+ F+D GG++LVAASS IG+ +RELGSECGIEFDEE+TAVIDHHN+D D
Sbjct: 64 FGGNINVETITAFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEERTAVIDHHNYDISD 123
Query: 464 DGKHTLIIADEANVAKIPIIVG-KVDSPVLFKGVGIAADDQNPLLINLLHASSSAYSFNP 640
G+HTLI+AD N+ K P IVG K +P+LF+GVG+ AD NPL++++L SS++YSF P
Sbjct: 124 PGQHTLIVADAENLLKAPTIVGKKALNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFP 183
Query: 641 DKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDDLFTASVEHA 790
DKP+ YP++VG NT LI LQARNNARV+F GSLD FSD F+++V+ A
Sbjct: 184 DKPITQYPHAVGKNTLLIAGLQARNNARVVFSGSLDFFSDAFFSSAVQKA 233
>sp|Q24319|OST48_DROME Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit) (DDOST 48 kDa subunit) (OST5OP) (DrOST)
(DmOST50)
Length = 449
Score = 284 bits (726), Expect = 2e-76
Identities = 146/263 (55%), Positives = 188/263 (71%), Gaps = 10/263 (3%)
Frame = +2
Query: 62 LFVSLCVSSCFAI-----DTLVLLETFATKETHSIFFKSLRERGHKLTYHLADDSSLILN 226
L L ++ C A+ +TLVLL+ A +ETHSIFFKSL++RG KLTY LADDSSL+L+
Sbjct: 7 LIAVLAIAHCQAVLETDANTLVLLDNLAIRETHSIFFKSLQDRGFKLTYKLADDSSLLLS 66
Query: 227 KFGEYLYKNVIIFAPSVREFGGKIDVPTLMNFVDEGGNILVAASSKIGEAIRELGSECGI 406
K+GEYLYKNVIIFAPSV EFGG + V L FVD+GGN+LVA S K G+A+RE SECG
Sbjct: 67 KYGEYLYKNVIIFAPSVEEFGGDVSVERLAQFVDDGGNVLVAGSEKSGDALREFASECGF 126
Query: 407 EFDEEKTAVIDHHNFDRKDDGKHTLIIADEANVAKIPIIVGKVD-----SPVLFKGVGIA 571
E DEE AVIDH ++D D G+HT I+ N+ + IVGK + +P+L++G G+
Sbjct: 127 ELDEENAAVIDHLHYDVSDAGEHTTILTSAKNLIQADTIVGKANRQADAAPLLYRGTGLI 186
Query: 572 ADDQNPLLINLLHASSSAYSFNPDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDL 751
AD +NPL++ LL A S+AYS+NP+ V DYP++VG T LI ALQARNNARV+F GSL
Sbjct: 187 ADKENPLVLKLLTAESTAYSYNPEASVSDYPHAVGRGTLLIAALQARNNARVVFSGSLLF 246
Query: 752 FSDDLFTASVEHAISKQTHARSG 820
FSD+ FT +V++A S H +G
Sbjct: 247 FSDESFTTAVQYAQSGVFHKLAG 269
>sp|P45971|OST48_CAEEL Probable dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 48 kDa subunit precursor
(Oligosaccharyl transferase 48 kDa subunit)
Length = 445
Score = 209 bits (531), Expect = 1e-53
Identities = 111/258 (43%), Positives = 157/258 (60%), Gaps = 3/258 (1%)
Frame = +2
Query: 56 LFLFVSLCVSSCFAIDTLVLLETFATKETHSIFFKSLRERGHKLTYHLADDSSLILNKFG 235
L L S+ A LVL ET A K+THS+F S++ERGH+LT ADDS L L K G
Sbjct: 7 LLLIASIGFHQSLADRVLVLGETAAVKDTHSVFLNSVKERGHELTVRAADDSQLALFKHG 66
Query: 236 EYLYKNVIIFAPSVREFGGKIDVPTLMNFVDEGGNILVAASSKIGEAIRELGSECGIEFD 415
+ ++ ++ I AP V+ FGG + + FVD GGN+LVAA S IG+A+RE+ +E G EF+
Sbjct: 67 QLIFDHLFILAPGVQVFGGSLSPSEISKFVDAGGNVLVAAGSNIGDALREIAAEHGFEFE 126
Query: 416 EEKTAVIDHHNFDRK-DDGKHTLIIADEANVAKIPIIVGKVDS--PVLFKGVGIAADDQN 586
E T+VIDHHN+D+ D G HT ++ + + +IVG PVLFKG+G+ A N
Sbjct: 127 EAGTSVIDHHNYDQTLDSGDHTTLVVGKDQLISAELIVGNSAKLHPVLFKGIGLVAGKTN 186
Query: 587 PLLINLLHASSSAYSFNPDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDDL 766
L ++++ AS +AYS++P P+ G T L+ LQ+RNNAR++F GS +LFS+
Sbjct: 187 NLALSIVRASGTAYSYDPKAVRATNPSIAGSRTLLVGGLQSRNNARIVFTGSSELFSNTF 246
Query: 767 FTASVEHAISKQTHARSG 820
F+A A+SG
Sbjct: 247 FSAKTNSVNPSVQGAQSG 264
>sp|Q29381|OST48_PIG Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit (Oligosaccharyl transferase 48 kDa subunit)
(DDOST 48 kDa subunit)
Length = 103
Score = 123 bits (308), Expect = 7e-28
Identities = 58/100 (58%), Positives = 77/100 (77%)
Frame = +2
Query: 104 TLVLLETFATKETHSIFFKSLRERGHKLTYHLADDSSLILNKFGEYLYKNVIIFAPSVRE 283
T VLL+ +ETHS+FF+SL++RG LT+ ADD SL L K+GE+LY N+I+F+PSV +
Sbjct: 4 TXVLLDNLNLRETHSLFFRSLKDRGFVLTFKTADDPSLSLIKYGEFLYDNLIVFSPSVED 63
Query: 284 FGGKIDVPTLMNFVDEGGNILVAASSKIGEAIRELGSECG 403
FGG I+V T+ F+D GG+ LVAASS IG+ +R LGSECG
Sbjct: 64 FGGNINVETISTFIDGGGSXLVAASSDIGDPLRXLGSECG 103
>sp|P33767|OSTB_YEAST Dolichyl-diphosphooligosaccharide--protein glycosyltransferase beta
subunit precursor (Oligosaccharyl transferase beta
subunit)
Length = 430
Score = 48.9 bits (115), Expect = 2e-05
Identities = 51/241 (21%), Positives = 104/241 (43%), Gaps = 5/241 (2%)
Frame = +2
Query: 53 FLFLFVSLCVSSCFAIDTLVLLETFATK-ETHSIFFKSLRERGHKLTYHLADDSSLILNK 229
F L ++ V TLVL + E +S++ K L +R +KL Y + +S ++
Sbjct: 9 FCILLQAIFVVGTQTSRTLVLYDQSTEPLEEYSVYLKDLEQRNYKLEYLDINSTSTTVDL 68
Query: 230 FG--EYLYKNVIIF-APSVREFGGKIDVPTLMNFVDEGGNILVAAS-SKIGEAIRELGSE 397
+ + L+ N+I+F + +I V L+ F + GNIL +S + IR +E
Sbjct: 69 YDKEQRLFDNIIVFPTKGGKNLARQIPVKQLIKFFENEGNILCMSSPGAVPNTIRLFLNE 128
Query: 398 CGIEFDEEKTAVIDHHNFDRKDDGKHTLIIADEANVAKIPIIVGKVDSPVLFKGVGIAAD 577
GI + + D+ + ++ ++ ++ + + +F A
Sbjct: 129 LGIYPSPKGHVIRDYFSPSSEE------LVVSSNHLLNKYVYNARKSEDFVFGESSAALL 182
Query: 578 DQNPLLINLLHASSSAYSFNPDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFS 757
+ ++ +L+A ++++ + K S G L+ Q NNAR++++GS D
Sbjct: 183 ENREQIVPILNAPRTSFTESKGKCNSWTSGSQGF---LVVGFQNLNNARLVWIGSSDFLK 239
Query: 758 D 760
+
Sbjct: 240 N 240
>sp|Q01811|VNSS_INSVN Nonstructural protein NS-S
Length = 449
Score = 35.4 bits (80), Expect = 0.19
Identities = 34/138 (24%), Positives = 58/138 (42%), Gaps = 15/138 (10%)
Frame = -2
Query: 420 SSSNSIPHSLPNSLIASPILELAATKILPPSST-KFIKVGTSIFPPNSLTDGANM----- 259
+++N + H +++S +L + +I P T +F + IFPPN+ + AN
Sbjct: 104 TNTNGVKHQGHLKVLSSQLLRMLEEQIAVPEITSRFGLKESDIFPPNNFIEAANKGSLSC 163
Query: 258 ---MTFLYKYSPN--LFKIS----EESSAKW*VNLCPRSRSDLKNIECVSLVANVSNSTK 106
+ F KYS N + K+S S +W L P N V+ +A S +
Sbjct: 164 VKEVLFDVKYSNNQSMGKVSVLSPTRSVHEWLYTLKPVFNQSQTNNRTVNTLAVKSLAMS 223
Query: 105 VSIAKHEETHKLTKRNKN 52
+ +TH + N N
Sbjct: 224 ATSDLMSDTHSFVRLNNN 241
>sp|Q6KHS4|RS7_MYCMO 30S ribosomal protein S7
Length = 156
Score = 35.4 bits (80), Expect = 0.19
Identities = 32/108 (29%), Positives = 50/108 (46%), Gaps = 2/108 (1%)
Frame = -2
Query: 366 ILELAATKILPPSSTKFIKVGTSIFPPNSLTDGANMMTFLYKYSPNLFKISEES-SAKW* 190
+ +LA I P K +VG G N Y+ + K +E+ S +W
Sbjct: 61 VFQLALDNITPQLEVKTRRVG-----------GTN-----YQVPMEVTKRRKETLSLRWL 104
Query: 189 VNLCPRSRSDLKNIECVSL-VANVSNSTKVSIAKHEETHKLTKRNKNF 49
++ R RS+ EC++ + + SN T SI K E+THK+ + NK F
Sbjct: 105 IHYS-RLRSEKTMQECLANEIIDASNKTGSSIKKREDTHKMAEANKAF 151
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 83,643,982
Number of Sequences: 369166
Number of extensions: 1637324
Number of successful extensions: 4756
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4558
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4724
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7859674995
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)