Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_010_L14
(529 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8TGI1|ATG1_PICPA Serine/threonine-protein kinase ATG1 (... 64 2e-10
sp|O13839|FIN1_SCHPO G2-specific protein kinase fin1 62 7e-10
sp|P32490|MKK1_YEAST MAP kinase kinase MKK1/SSP32 62 1e-09
sp|P27636|CDC15_YEAST Cell division control protein 15 62 1e-09
sp|P13677|KPC2_DROME Protein kinase C, eye isozyme (PKC) (d... 61 1e-09
sp|Q8TFN2|ATG1_PICAN Serine/threonine-protein kinase ATG1 (... 61 1e-09
sp|Q21734|KS6A_CAEEL Putative ribosomal protein S6 kinase a... 61 2e-09
sp|P09215|KPCD_RAT Protein kinase C, delta type (nPKC-delta) 60 2e-09
sp|Q5PU49|KPCD_CANFA Protein kinase C, delta type (nPKC-delta) 60 2e-09
sp|Q5A649|ATG1_CANAL Serine/threonine-protein kinase ATG1 (... 60 2e-09
>sp|Q8TGI1|ATG1_PICPA Serine/threonine-protein kinase ATG1 (Autophagy-related protein 1)
(Glucose-induced selective autophagy protein 10)
(Pexophagy zeocin-resistant mutant protein 1)
Length = 796
Score = 63.9 bits (154), Expect = 2e-10
Identities = 30/68 (44%), Positives = 43/68 (63%)
Frame = +2
Query: 23 IVKIADFGTSRFLNVDSKYQKKTFCGTANYMSPEVMQGEPYGCSADIWSLGATVFEMATE 202
++KIADFG +RFL S +T CG+ YM+PE+++ E Y AD+WS+GA V+EM+
Sbjct: 185 VLKIADFGFARFLPATS--MAETLCGSPLYMAPEILRYEKYNAKADLWSVGAVVYEMSVG 242
Query: 203 NARLCKHN 226
HN
Sbjct: 243 TPPFPAHN 250
>sp|O13839|FIN1_SCHPO G2-specific protein kinase fin1
Length = 722
Score = 62.4 bits (150), Expect = 7e-10
Identities = 25/64 (39%), Positives = 45/64 (70%)
Frame = +2
Query: 2 ILINKNNIVKIADFGTSRFLNVDSKYQKKTFCGTANYMSPEVMQGEPYGCSADIWSLGAT 181
I +++NN VK+ DFG S+ L+ +++ +++ GT YMSPE+++ PY +D+W+LG
Sbjct: 157 IFLDENNSVKLGDFGLSKLLD-NTRVFTQSYVGTPYYMSPEIIRSSPYSAKSDVWALGCV 215
Query: 182 VFEM 193
+FE+
Sbjct: 216 IFEI 219
>sp|P32490|MKK1_YEAST MAP kinase kinase MKK1/SSP32
Length = 508
Score = 61.6 bits (148), Expect = 1e-09
Identities = 32/65 (49%), Positives = 45/65 (69%)
Frame = +2
Query: 2 ILINKNNIVKIADFGTSRFLNVDSKYQKKTFCGTANYMSPEVMQGEPYGCSADIWSLGAT 181
IL+N+N VK+ DFG S V+S TF GT+ YM+PE +QG+PY ++D+WSLG T
Sbjct: 355 ILLNENGQVKLCDFGVSGEA-VNSL--ATTFTGTSFYMAPERIQGQPYSVTSDVWSLGLT 411
Query: 182 VFEMA 196
+ E+A
Sbjct: 412 ILEVA 416
>sp|P27636|CDC15_YEAST Cell division control protein 15
Length = 974
Score = 61.6 bits (148), Expect = 1e-09
Identities = 34/76 (44%), Positives = 46/76 (60%)
Frame = +2
Query: 2 ILINKNNIVKIADFGTSRFLNVDSKYQKKTFCGTANYMSPEVMQGEPYGCSADIWSLGAT 181
IL++ +N VK+ADFG S +N + T GT N+M+PE++ +DIWSLGAT
Sbjct: 152 ILLSADNTVKLADFGVSTIVNSSAL----TLAGTLNWMAPEILGNRGASTLSDIWSLGAT 207
Query: 182 VFEMATENARLCKHNL 229
V EM T+N HNL
Sbjct: 208 VVEMLTKNPPY--HNL 221
Score = 37.0 bits (84), Expect = 0.029
Identities = 18/60 (30%), Positives = 31/60 (51%)
Frame = +3
Query: 297 HPPYHEMIDNAVIFKIGNERKIEFPSNMPDDIKGFLNLMIIAEPKNRSTADSLLNCEFLN 476
+PPYH + D + + + N+ PS+ + +K FL+ + R TAD LL ++N
Sbjct: 215 NPPYHNLTDANIYYAVENDTYYP-PSSFSEPLKDFLSKCFVKNMYKRPTADQLLKHVWIN 273
>sp|P13677|KPC2_DROME Protein kinase C, eye isozyme (PKC) (dPKC53E(EY)) (Protein INAC)
(Inactivation no after-potential C protein)
(Photoreceptor-specific PKC) (Eye-PKC)
Length = 700
Score = 61.2 bits (147), Expect = 1e-09
Identities = 29/69 (42%), Positives = 41/69 (59%)
Frame = +2
Query: 2 ILINKNNIVKIADFGTSRFLNVDSKYQKKTFCGTANYMSPEVMQGEPYGCSADIWSLGAT 181
IL++ VK+ DFG S+ V + +TFCGT NYM+PE++ +PY +AD WS G
Sbjct: 501 ILLDGEGHVKLVDFGLSKE-GVTERQTTRTFCGTPNYMAPEIVSYDPYSIAADWWSFGVL 559
Query: 182 VFEMATENA 208
+FE A
Sbjct: 560 LFEFMAGQA 568
>sp|Q8TFN2|ATG1_PICAN Serine/threonine-protein kinase ATG1 (Autophagy-related protein 1)
(Peroxisome degradation deficient protein 7)
Length = 804
Score = 61.2 bits (147), Expect = 1e-09
Identities = 27/58 (46%), Positives = 41/58 (70%)
Frame = +2
Query: 23 IVKIADFGTSRFLNVDSKYQKKTFCGTANYMSPEVMQGEPYGCSADIWSLGATVFEMA 196
++KIADFG +RFL S +T CG+ YM+PE+++ E Y AD+WS+GA ++EM+
Sbjct: 189 VLKIADFGFARFL--PSTSMAETLCGSPLYMAPEILRYEKYNAKADLWSVGAVIYEMS 244
>sp|Q21734|KS6A_CAEEL Putative ribosomal protein S6 kinase alpha
Length = 784
Score = 60.8 bits (146), Expect = 2e-09
Identities = 28/66 (42%), Positives = 43/66 (65%)
Frame = +2
Query: 2 ILINKNNIVKIADFGTSRFLNVDSKYQKKTFCGTANYMSPEVMQGEPYGCSADIWSLGAT 181
IL++ + +K+ DFG S+ +DS+ + +FCGT YM+PEV+ + +AD WSLG
Sbjct: 235 ILLDADGHIKVTDFGLSKEA-IDSEKKTYSFCGTVEYMAPEVINRRGHSMAADFWSLGVL 293
Query: 182 VFEMAT 199
+FEM T
Sbjct: 294 MFEMLT 299
Score = 40.4 bits (93), Expect = 0.003
Identities = 20/58 (34%), Positives = 34/58 (58%)
Frame = +2
Query: 26 VKIADFGTSRFLNVDSKYQKKTFCGTANYMSPEVMQGEPYGCSADIWSLGATVFEMAT 199
++I DFG ++ ++ T C TA +++PEV++ + Y S D+WSLG + M T
Sbjct: 590 LRIVDFGFAKQSRAENG-MLMTPCYTAQFVAPEVLRKQGYDRSCDVWSLGVLLHTMLT 646
>sp|P09215|KPCD_RAT Protein kinase C, delta type (nPKC-delta)
Length = 673
Score = 60.5 bits (145), Expect = 2e-09
Identities = 26/64 (40%), Positives = 43/64 (67%)
Frame = +2
Query: 2 ILINKNNIVKIADFGTSRFLNVDSKYQKKTFCGTANYMSPEVMQGEPYGCSADIWSLGAT 181
++++K+ +KIADFG + N+ + + TFCGT +Y++PE++QG Y S D WS G
Sbjct: 477 VMLDKDGHIKIADFGMCKE-NIFGENRASTFCGTPDYIAPEILQGLKYSFSVDWWSFGVL 535
Query: 182 VFEM 193
++EM
Sbjct: 536 LYEM 539
>sp|Q5PU49|KPCD_CANFA Protein kinase C, delta type (nPKC-delta)
Length = 674
Score = 60.5 bits (145), Expect = 2e-09
Identities = 26/64 (40%), Positives = 43/64 (67%)
Frame = +2
Query: 2 ILINKNNIVKIADFGTSRFLNVDSKYQKKTFCGTANYMSPEVMQGEPYGCSADIWSLGAT 181
++++++ +KIADFG + N+ + Q TFCGT +Y++PE++QG Y S D WS G
Sbjct: 477 VMLDQDGHIKIADFGMCKE-NIFGEKQASTFCGTPDYIAPEILQGLKYSFSVDWWSFGVL 535
Query: 182 VFEM 193
++EM
Sbjct: 536 LYEM 539
>sp|Q5A649|ATG1_CANAL Serine/threonine-protein kinase ATG1 (Autophagy-related protein 1)
Length = 834
Score = 60.5 bits (145), Expect = 2e-09
Identities = 28/57 (49%), Positives = 40/57 (70%)
Frame = +2
Query: 23 IVKIADFGTSRFLNVDSKYQKKTFCGTANYMSPEVMQGEPYGCSADIWSLGATVFEM 193
I+KIADFG +RFL S +T CG+ YM+PE+++ E Y AD+WS+GA ++EM
Sbjct: 235 ILKIADFGFARFL--PSTSMAETLCGSPLYMAPEILRYEKYNAKADLWSVGAVLYEM 289
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 56,316,379
Number of Sequences: 369166
Number of extensions: 1079467
Number of successful extensions: 4646
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3878
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4069
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3551563800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)