Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_010_J06
(902 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O95202|LETM1_HUMAN Leucine zipper-EF-hand containing tra... 179 9e-45
sp|Q9Z2I0|LETM1_MOUSE Leucine zipper-EF-hand containing tra... 172 9e-43
sp|Q5XIN6|LETM1_RAT Leucine zipper-EF-hand containing trans... 171 3e-42
sp|P91927|A60DA_DROME Calcium-binding mitochondrial protein... 162 2e-39
sp|O13920|YDWH_SCHPO Hypothetical protein C23C11.17 in chro... 84 4e-16
sp|Q08179|MDM38_YEAST Mitochondrial distribution and morpho... 70 8e-12
sp|Q22866|TPM1_CAEEL Tropomyosin isoforms 1/2 41 0.004
sp|P15846|TPMM_TRICO Tropomyosin, muscle 41 0.005
sp|P25386|USO1_YEAST Intracellular protein transport protei... 40 0.007
sp|P49454|CENPF_HUMAN CENP-F kinetochore protein (Centromer... 40 0.009
>sp|O95202|LETM1_HUMAN Leucine zipper-EF-hand containing transmembrane protein 1,
mitochondrial precursor
Length = 739
Score = 179 bits (454), Expect = 9e-45
Identities = 107/298 (35%), Positives = 171/298 (57%), Gaps = 4/298 (1%)
Frame = +2
Query: 14 EVRETGQIARTDDIVKFSRLFNDEITLASLDHHQLRALCKLLEIASIGPKLVLISRLKMK 193
++RETG+ ++I++FS+LF DE+TL +L QL ALCKLLE+ SIG L +L M+
Sbjct: 292 KIRETGERPSNEEIMRFSKLFEDELTLDNLTRPQLVALCKLLELQSIGTNNFLRFQLTMR 351
Query: 194 VYILKVDDRMIIKEGIDTIPTFELQQLCRERGMRAFGISEDRLRFQLSQWCNLSVEKQVP 373
+ +K DD++I +EG+D++ ELQ CR RGMRA G++EDRLR QL QW +L + +++P
Sbjct: 352 LRSIKADDKLIAEEGVDSLNVKELQAACRARGMRALGVTEDRLRGQLKQWLDLHLHQEIP 411
Query: 374 ISLLLLSRIMYLPENLSPEQQLNEVIFTLPPKVSKDVILR-SSTNDENVDSEPKFESITE 550
SLL+LSR MYLP+ LSP QL + TLP V+K+ ++ + E VD++ K E+ +
Sbjct: 412 TSLLILSRAMYLPDTLSPADQLKSTLQTLPEIVAKEAQVKVAEVEGEQVDNKAKLEATLQ 471
Query: 551 ELEHILTEVKEAERIEKETLIDR---AHQINIDAQDETTTKEKQLSGESISISDLNQIET 721
E I E +E E ++K + + + ++ Q T + ++ + L
Sbjct: 472 EEAAIQQEHREKE-LQKRSEVAKDFEPERVVAAPQRPGTEPQPEMPDTVLQSETLKDTAP 530
Query: 722 VIENLTSSETTDLEGLDKLNEKAASLQEEDQLTERLKSRNRHTKKRMRNACLQLYSFK 895
V+E L E T E +D L++ + LQE+ + + K K+ +++ L K
Sbjct: 531 VLEGLKEEEITK-EEIDILSDACSKLQEQKKSLTKEKEELELLKEDVQDYSEDLQEIK 587
>sp|Q9Z2I0|LETM1_MOUSE Leucine zipper-EF-hand containing transmembrane protein 1,
mitochondrial precursor
Length = 738
Score = 172 bits (437), Expect = 9e-43
Identities = 106/297 (35%), Positives = 165/297 (55%), Gaps = 3/297 (1%)
Frame = +2
Query: 14 EVRETGQIARTDDIVKFSRLFNDEITLASLDHHQLRALCKLLEIASIGPKLVLISRLKMK 193
++RETG+ ++I++FS+LF DE+TL +L QL ALCKLLE+ SIG L +L M+
Sbjct: 291 KIRETGERPSNEEIMRFSKLFEDELTLDNLTRPQLVALCKLLELQSIGTNNFLRFQLTMR 350
Query: 194 VYILKVDDRMIIKEGIDTIPTFELQQLCRERGMRAFGISEDRLRFQLSQWCNLSVEKQVP 373
+ +K DD++I +EG+D++ ELQ CR RGMRA G++EDRL+ QL QW +L + ++P
Sbjct: 351 LRSIKADDKLISEEGVDSLTVKELQAACRARGMRALGVTEDRLKGQLKQWLDLHLHHEIP 410
Query: 374 ISLLLLSRIMYLPENLSPEQQLNEVIFTLPPKVSKDVILR-SSTNDENVDSEPKFESITE 550
SLL+LSR MYLP+ LSP QL + TLP V+K+ ++ + E VD++ K E+ +
Sbjct: 411 TSLLILSRAMYLPDTLSPADQLKSTLQTLPEIVAKEAQVKVAEVEGEKVDNKAKLEATLQ 470
Query: 551 ELEHILTEVKEAERIEKETLIDRAHQINIDAQDETTTKEKQLSGESISISD--LNQIETV 724
E I E E + E + D ++ E Q + + + L V
Sbjct: 471 EEAAIQQEHLEELKRASEAVKDIQPEVAEATLPGRPGPEPQPPVDDVILPSEVLTDTAPV 530
Query: 725 IENLTSSETTDLEGLDKLNEKAASLQEEDQLTERLKSRNRHTKKRMRNACLQLYSFK 895
+E L E T E +D L++ + LQE+ + + K K+ +++ L K
Sbjct: 531 LEGLKGEEITK-EEIDILSDACSKLQEQKKSLTKEKEELELLKEDVQDYSEDLQEIK 586
>sp|Q5XIN6|LETM1_RAT Leucine zipper-EF-hand containing transmembrane protein 1,
mitochondrial precursor
Length = 739
Score = 171 bits (432), Expect = 3e-42
Identities = 110/286 (38%), Positives = 165/286 (57%), Gaps = 13/286 (4%)
Frame = +2
Query: 14 EVRETGQIARTDDIVKFSRLFNDEITLASLDHHQLRALCKLLEIASIGPKLVLISRLKMK 193
++RETG+ ++I++FS+LF DE+TL +L QL ALCKLLE+ SIG L +L M+
Sbjct: 291 KIRETGERPSNEEIMRFSKLFEDELTLDNLTRPQLVALCKLLELQSIGTNNFLRFQLTMR 350
Query: 194 VYILKVDDRMIIKEGIDTIPTFELQQLCRERGMRAFGISEDRLRFQLSQWCNLSVEKQVP 373
+ +K DD++I +EG+D++ ELQ CR RGMRA G++EDRL+ QL QW +L + ++P
Sbjct: 351 LRSIKADDKLISEEGVDSLTVKELQAACRARGMRALGVTEDRLKGQLKQWLDLHLYHEIP 410
Query: 374 ISLLLLSRIMYLPENLSPEQQLNEVIFTLPPKVSKDVILRSS-TNDENVDSEPKFESITE 550
SLL+LSR MYLP+ LSP QL + TLP V+K+ ++++ E VD++ K E+ +
Sbjct: 411 TSLLILSRAMYLPDTLSPADQLKSTLQTLPEIVAKEAQVKAAEVEGEQVDNKAKLEATLQ 470
Query: 551 E-----LEHILTEVKEAERIEKETLIDRAHQINIDAQDETTTKEKQLSGESISISD-LNQ 712
E EH L E+K A K+ + A + + + + K + + I S+ L
Sbjct: 471 EEAAIQQEH-LEELKRAAETAKDIQPEVA-EATVPGRPGAELQPKMV--DVIPPSEILKD 526
Query: 713 IETVIENLTSSETTDLE------GLDKLNEKAASLQEEDQLTERLK 832
V+E L E T E KL E+ SL +E + E LK
Sbjct: 527 TAPVLEGLKGEEITKEEIDILSDACSKLKEQKKSLTKEKEELELLK 572
>sp|P91927|A60DA_DROME Calcium-binding mitochondrial protein Anon-60Da
Length = 1013
Score = 162 bits (409), Expect = 2e-39
Identities = 102/306 (33%), Positives = 173/306 (56%), Gaps = 20/306 (6%)
Frame = +2
Query: 14 EVRETGQIARTDDIVKFSRLFNDEITLASLDHHQLRALCKLLEIASIGPKLVLISRLKMK 193
++R + D+I+KF++ F+DEITL SL QL ALC++LE+ +IG +L +L++K
Sbjct: 320 KIRNPTEPVSNDEIIKFAKRFDDEITLDSLSREQLAALCRVLELNTIGTTTLLRFQLRLK 379
Query: 194 VYILKVDDRMIIKEGIDTIPTFELQQLCRERGMRAFGISEDRLRFQLSQWCNLSVEKQVP 373
+ L DDR+I +EG+D++ ELQQ C+ RGMRA+G++E+RLRFQL +W +LS+ +QVP
Sbjct: 380 LRSLATDDRVIAREGVDSLDLLELQQACKARGMRAYGLTEERLRFQLKEWIDLSLNEQVP 439
Query: 374 ISLLLLSRIMYLPENLSPEQQLNEVIFTLPPKVSKDVILRSSTNDENVDSEPKFESITEE 553
+LLLLSR M + ++ +L E I LP V ++ VD++ K E I EE
Sbjct: 440 PTLLLLSRTMLISDDSITTDKLKETIRVLPDAVGAHTRHAIGESEGKVDNKTKIEIIKEE 499
Query: 554 LEHILTEVKEAERIEKETLIDRA------------------HQINIDAQDETTTKEKQLS 679
E + E +E ER +ET+ R+ Q +D +++++ E +
Sbjct: 500 -ERKIREEREEER--EETIAKRSAIKEEIPAPYVFAEKLSGSQDLLDHKEQSSVSE---T 553
Query: 680 GESISISDLNQIETVIENLTSSE--TTDLEGLDKLNEKAASLQEEDQLTERLKSRNRHTK 853
+ IS +D+ + ++ L+S + + E + +L E+ A +E+ E L+ + K
Sbjct: 554 DKGISSTDVQLLSEALKTLSSDKQLVVEKETIKELKEELADYKED---VEELREVRQVVK 610
Query: 854 KRMRNA 871
+ +R +
Sbjct: 611 EPVRES 616
>sp|O13920|YDWH_SCHPO Hypothetical protein C23C11.17 in chromosome I
Length = 485
Score = 84.3 bits (207), Expect = 4e-16
Identities = 59/229 (25%), Positives = 112/229 (48%), Gaps = 4/229 (1%)
Frame = +2
Query: 14 EVRETGQIARTDDIVKFSRLFNDEITLASLDHHQLRALCKLLEIASIGPKLVLISRLKMK 193
+VR +GQ +++++ + F D+ITL +L QL A+C+ + + + G +L ++ +
Sbjct: 256 KVRTSGQSPSREELIEVCKYFKDDITLDNLSRAQLVAMCRYMNLNAFGTDPLLRYNIRHR 315
Query: 194 VYILKVDDRMIIKEGIDTIPTFELQQLCRERGMRAFGISEDRLRFQLSQWCNLSVEKQVP 373
+ ++ DDR I EGI+++ EL C RG+R G+S +L+ +LS W ++ ++ +P
Sbjct: 316 MRQIRRDDRAIYIEGINSLSIPELFNACNSRGIRTQGLSPAKLKEELSVWLDMRIKHGIP 375
Query: 374 ISLLLLSRIMYLPENL----SPEQQLNEVIFTLPPKVSKDVILRSSTNDENVDSEPKFES 541
+L+LS N S L + + ++P ++ + + VD K S
Sbjct: 376 SVILMLSNAFSYGYNEGTYDSRWDALQDTLASIPDELYHETV---------VDMPTKQVS 426
Query: 542 ITEELEHILTEVKEAERIEKETLIDRAHQINIDAQDETTTKEKQLSGES 688
E LE IL E +E E E + + ++ T + +S +S
Sbjct: 427 NKERLE-ILREQEELIEEEAEHVAEHPDLAKKQTEENKATSKPAVSAKS 474
>sp|Q08179|MDM38_YEAST Mitochondrial distribution and morphology protein 38, mitochondrial
precursor
Length = 573
Score = 70.1 bits (170), Expect = 8e-12
Identities = 61/262 (23%), Positives = 120/262 (45%), Gaps = 2/262 (0%)
Frame = +2
Query: 47 DDIVKFSRLFNDEITLASLDHHQLRALCKLLEIASIGPKLVLISRLKMKVYILKVDDRMI 226
D+I +++F ++ L +L QL A+ K + + G +L +++ K+ + DD+ I
Sbjct: 239 DEISAIAQMFKNDSVLDNLSRPQLAAMSKFMSLRPFGNDNMLRYQIRSKLKDIMNDDKTI 298
Query: 227 IKEGIDTIPTFELQQLCRERGMRAFGISEDRLRFQLSQWCNLSVEKQVPISLLLLSRIMY 406
EG++++ EL Q C RGM+A+G+S++ L L W L + +++P L++LS
Sbjct: 299 DYEGVESLSQEELYQACVSRGMKAYGVSKEDLVDNLKVWLELRLRQKIPSVLMVLSSTFT 358
Query: 407 LPENLSPEQQLNEVIFTLPPKVSKDVILRSSTNDENVDSEPKFESITEELEHILTEVKEA 586
P++ ++ L K S D+ +D ++ I + L I V
Sbjct: 359 F--GGLPKENYSKAFSPLAEKKE-----TKSKYDDLLDL--YYDGILQVLSSIPDPVYNV 409
Query: 587 ERIEKETLIDRAHQINIDAQ--DETTTKEKQLSGESISISDLNQIETVIENLTSSETTDL 760
+++ A + + Q ++ E++ +I + E+VI S+ T L
Sbjct: 410 AKLDVSESKSSAAETEAEKQVAEKKIKTEEKPEETAIPKEEATAKESVIATTASAVTPKL 469
Query: 761 EGLDKLNEKAASLQEEDQLTER 826
+NEKA + + E+ E+
Sbjct: 470 V---VVNEKAETAKTEEISQEK 488
>sp|Q22866|TPM1_CAEEL Tropomyosin isoforms 1/2
Length = 284
Score = 41.2 bits (95), Expect = 0.004
Identities = 34/142 (23%), Positives = 68/142 (47%), Gaps = 3/142 (2%)
Frame = +2
Query: 467 KVSKDVILRSSTNDENVDSEPKFESITEELEHILTEVKEAERIEKETL--IDRAHQINID 640
K+ KD L D +E K ITE+LE + E+++ ++ +T +D+A +
Sbjct: 12 KIEKDNAL-----DRADAAEEKVRQITEKLERVEEELRDTQKKMTQTGDDLDKAQEDLSA 66
Query: 641 AQDETTTKEKQLSGESISISDLNQIETVI-ENLTSSETTDLEGLDKLNEKAASLQEEDQL 817
A + KEK + ++ LN+ T++ E L +E +KL E ++ E +++
Sbjct: 67 ATSKLEEKEKTVQEAEAEVASLNRRMTLLEEELERAEERLKIATEKLEEATHNVDESERV 126
Query: 818 TERLKSRNRHTKKRMRNACLQL 883
+ +++R+ ++R QL
Sbjct: 127 RKVMENRSLQDEERANTVEAQL 148
>sp|P15846|TPMM_TRICO Tropomyosin, muscle
Length = 284
Score = 40.8 bits (94), Expect = 0.005
Identities = 33/142 (23%), Positives = 69/142 (48%), Gaps = 3/142 (2%)
Frame = +2
Query: 467 KVSKDVILRSSTNDENVDSEPKFESITEELEHILTEVKEAER--IEKETLIDRAHQINID 640
K+ KD L D +E K ITE+LE + E+++ ++ ++ E +D+A +
Sbjct: 12 KIEKDNAL-----DRADAAEEKVRQITEKLERVEEELRDTQKKMMQTENDLDKAQEDLAA 66
Query: 641 AQDETTTKEKQLSGESISISDLNQIETVI-ENLTSSETTDLEGLDKLNEKAASLQEEDQL 817
A + KEK++ ++ LN+ T++ E L +E +KL E ++ E +++
Sbjct: 67 ATSQLEEKEKKVQEAEAEVAALNRRMTLLEEELERAEERLKIATEKLEEATHNVDESERV 126
Query: 818 TERLKSRNRHTKKRMRNACLQL 883
+ +++ + ++R QL
Sbjct: 127 RKVMENGSFQDEERANTIEAQL 148
>sp|P25386|USO1_YEAST Intracellular protein transport protein USO1 (Int-1)
Length = 1790
Score = 40.4 bits (93), Expect = 0.007
Identities = 44/221 (19%), Positives = 96/221 (43%), Gaps = 22/221 (9%)
Frame = +2
Query: 305 ISEDRLRFQLSQWCNLSVEKQVPISLLLLSRIMYLPENLSPEQQLNEVIFTLPPKVSKDV 484
+S+++ FQ+ + S+EK + +S + E + + ++ + + K+
Sbjct: 1006 MSQEKENFQIERG---SIEKNIEQLKKTISDLEQTKEEIISKSDSSKDEYESQISLLKEK 1062
Query: 485 ILRSST-NDENVDSEPKFESITEELEHIL-----------TEVKEAERIEKETLIDRAH- 625
+ ++T NDENV+ + EELE L T+++ +E+ KE + H
Sbjct: 1063 LETATTANDENVNKISELTKTREELEAELAAYKNLKNELETKLETSEKALKEVKENEEHL 1122
Query: 626 -QINIDAQDETTTKEKQLSGESISISDLN--------QIETVIENLTSSETTDLEGLDKL 778
+ I + E T ++QL+ ++ L Q++ E + + E E + +L
Sbjct: 1123 KEEKIQLEKEATETKQQLNSLRANLESLEKEHEDLAAQLKKYEEQIANKERQYNEEISQL 1182
Query: 779 NEKAASLQEEDQLTERLKSRNRHTKKRMRNACLQLYSFKSS 901
N++ S Q+E++ ++ K M++ + + K S
Sbjct: 1183 NDEITSTQQENESIKKKNDELEGEVKAMKSTSEEQSNLKKS 1223
Score = 35.4 bits (80), Expect = 0.22
Identities = 35/157 (22%), Positives = 74/157 (47%), Gaps = 12/157 (7%)
Frame = +2
Query: 437 LNEVIFTLPPKVSKDVILRSSTNDENVDSEPKFESITEELEHILTEVKEAERIEKETLID 616
LNE T+ + S+ + ++ DE + + + E +E+++ +E+++ E L +
Sbjct: 1372 LNEGSSTITQEYSEKI---NTLEDELIRLQNENELKAKEIDNTRSELEKVSLSNDELLEE 1428
Query: 617 RAHQINIDAQDE--------TTTKEKQLSGESISISDLNQIETVIENLTSSETTDLEGLD 772
+ + I QDE T EK LS E + DL ++ + S+ EGL
Sbjct: 1429 KQNTIK-SLQDEILSYKDKITRNDEKLLSIERDNKRDLESLKEQLRAAQESKAKVEEGLK 1487
Query: 773 KLNEKA----ASLQEEDQLTERLKSRNRHTKKRMRNA 871
KL E++ A L++ ++ ++L+S + ++++
Sbjct: 1488 KLEEESSKEKAELEKSKEMMKKLESTIESNETELKSS 1524
>sp|P49454|CENPF_HUMAN CENP-F kinetochore protein (Centromere protein F) (Mitosin) (AH
antigen)
Length = 3210
Score = 40.0 bits (92), Expect = 0.009
Identities = 41/164 (25%), Positives = 74/164 (45%), Gaps = 3/164 (1%)
Frame = +2
Query: 401 MYLPENLSPEQQLNEVIFTLPPKVSKDVILRSSTNDENVDSEPKFESITEELEHILTEVK 580
+ L E S + QL E+ ++ L+ S + + D K + EE+ +
Sbjct: 2748 LLLEEIKSSKDQLKELTL-------ENSELKKSLDCMHKDQVEKEGKVREEIAEYQLRLH 2800
Query: 581 EAERIEKETLIDRAHQINIDAQ---DETTTKEKQLSGESISISDLNQIETVIENLTSSET 751
EAE+ + L+D Q ++ Q ++ T+KE+ LS + + I L + + N + T
Sbjct: 2801 EAEKKHQALLLDTNKQYEVEIQTYREKLTSKEECLSSQKLEIDLLKSSKEELNNSLKATT 2860
Query: 752 TDLEGLDKLNEKAASLQEEDQLTERLKSRNRHTKKRMRNACLQL 883
LE L K K +L+ +QL ++ R + K + +C QL
Sbjct: 2861 QILEELKK--TKMDNLKYVNQL-KKENERAQGKMKLLIKSCKQL 2901
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 91,111,556
Number of Sequences: 369166
Number of extensions: 1767781
Number of successful extensions: 6725
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6298
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6690
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9126484700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)