Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_010_I21
(787 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9SZ67|WRK19_ARATH Probable WRKY transcription factor 19... 97 4e-20
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 95 2e-19
sp|Q13043|STK4_HUMAN Serine/threonine-protein kinase 4 (STE... 93 7e-19
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 92 1e-18
sp|Q9JI10|STK3_MOUSE Serine/threonine-protein kinase 3 (STE... 92 2e-18
sp|Q13188|STK3_HUMAN Serine/threonine-protein kinase 3 (STE... 91 3e-18
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 90 8e-18
sp|P50528|PLO1_SCHPO Serine/threonine-protein kinase plo1 89 1e-17
sp|P38692|KIC1_YEAST Serine/threonine-protein kinase KIC1 (... 87 4e-17
sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinas... 87 4e-17
>sp|Q9SZ67|WRK19_ARATH Probable WRKY transcription factor 19 (WRKY DNA-binding protein 19)
Length = 1895
Score = 97.4 bits (241), Expect = 4e-20
Identities = 66/194 (34%), Positives = 101/194 (52%), Gaps = 5/194 (2%)
Frame = +3
Query: 213 EEENSVFIVMERMAS-SLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKILHRSLNC 389
++E++++I +E + SLR L + + L + + Y+ +ILDGL Y+H +HR++ C
Sbjct: 1695 KDESNLYIFLELVTQGSLRKLYQRN--QLGDSVVSLYTRQILDGLKYLHDKGFIHRNIKC 1752
Query: 390 GSVLIDFNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAPEVLLQ----ESY 557
+VL+D N KL +F L+K + T W+APEV+L + Y
Sbjct: 1753 ANVLVDANGTVKLADFGLAKVMSLWRTPYW-------------NWMAPEVILNPKDYDGY 1799
Query: 558 GFKADIWSFGVTMLEMMTGRRPYPQLNNVQAAHRIVADRHIEFDRDLYPNSGFDEILDTC 737
G ADIWS G T+LEM+TG+ PY L A + I + + D+ D IL TC
Sbjct: 1800 GTPADIWSLGCTVLEMLTGQIPYSDLEIGTALYNIGTGKLPKIP-DILSLDARDFIL-TC 1857
Query: 738 LRSNQNERPNVFDL 779
L+ N ERP +L
Sbjct: 1858 LKVNPEERPTAAEL 1871
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 94.7 bits (234), Expect = 2e-19
Identities = 61/198 (30%), Positives = 100/198 (50%), Gaps = 2/198 (1%)
Frame = +3
Query: 192 VQLLSMYEEENSVFIVMERMASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKIL 371
V+ L E+ ++ I++E + S L E ++ Y+ ++L GL Y+H I+
Sbjct: 134 VRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRTYTNQLLLGLEYLHNHAIM 193
Query: 372 HRSLNCGSVLIDFNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAPEVLLQE 551
HR + ++L+D KL +F SK + + +T K +KG+ W+APEV+LQ
Sbjct: 194 HRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKGTPY-----WMAPEVILQT 248
Query: 552 SYGFKADIWSFGVTMLEMMTGRRPYPQLNNVQAA--HRIVADRHIEFDRDLYPNSGFDEI 725
+ F ADIWS G T++EM+TG+ P+ Q AA H H ++ +S ++
Sbjct: 249 GHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHPPIPDNI--SSDANDF 306
Query: 726 LDTCLRSNQNERPNVFDL 779
L CL+ N RP +L
Sbjct: 307 LLKCLQQEPNLRPTASEL 324
>sp|Q13043|STK4_HUMAN Serine/threonine-protein kinase 4 (STE20-like kinase MST1) (MST-1)
(Mammalian STE20-like protein kinase 1)
(Serine/threonine-protein kinase Krs-2)
Length = 487
Score = 93.2 bits (230), Expect = 7e-19
Identities = 56/202 (27%), Positives = 99/202 (49%), Gaps = 1/202 (0%)
Frame = +3
Query: 177 DTSRFVQLLSMYEEENSVFIVMERM-ASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYM 353
D+ V+ Y + ++IVME A S+ +++ + LTED+I L GL Y+
Sbjct: 81 DSPHVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLTEDEIATILQSTLKGLEYL 140
Query: 354 HKLKILHRSLNCGSVLIDFNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAP 533
H ++ +HR + G++L++ +AKL +F ++ L D K + + W+AP
Sbjct: 141 HFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMA-------KRNTVIGTPFWMAP 193
Query: 534 EVLLQESYGFKADIWSFGVTMLEMMTGRRPYPQLNNVQAAHRIVADRHIEFDRDLYPNSG 713
EV+ + Y ADIWS G+T +EM G+ PY ++ ++A I + F + +
Sbjct: 194 EVIQEIGYNCVADIWSLGITAIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDN 253
Query: 714 FDEILDTCLRSNQNERPNVFDL 779
F + + CL + +R L
Sbjct: 254 FTDFVKQCLVKSPEQRATATQL 275
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 92.4 bits (228), Expect = 1e-18
Identities = 59/196 (30%), Positives = 97/196 (49%)
Frame = +3
Query: 192 VQLLSMYEEENSVFIVMERMASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKIL 371
V+ L E++++ I++E + S L E ++ Y+ ++L GL Y+H I+
Sbjct: 135 VRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYTRQLLLGLEYLHNHAIM 194
Query: 372 HRSLNCGSVLIDFNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAPEVLLQE 551
HR + ++L+D KL +F SK + + +T K +KG+ W+APEV+LQ
Sbjct: 195 HRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTPY-----WMAPEVILQT 249
Query: 552 SYGFKADIWSFGVTMLEMMTGRRPYPQLNNVQAAHRIVADRHIEFDRDLYPNSGFDEILD 731
+ F ADIWS G T++EM+TG+ P+ Q AA + +S + L
Sbjct: 250 GHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPDTLSSDAKDFLL 309
Query: 732 TCLRSNQNERPNVFDL 779
CL+ N RP +L
Sbjct: 310 KCLQEVPNLRPTASEL 325
>sp|Q9JI10|STK3_MOUSE Serine/threonine-protein kinase 3 (STE20-like kinase MST2) (MST-2)
(Mammalian STE20-like protein kinase 2)
Length = 497
Score = 91.7 bits (226), Expect = 2e-18
Identities = 56/202 (27%), Positives = 99/202 (49%), Gaps = 1/202 (0%)
Frame = +3
Query: 177 DTSRFVQLLSMYEEENSVFIVMERM-ASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYM 353
D+ V+ Y + ++IVME A S+ +++ + LTED+I L GL Y+
Sbjct: 78 DSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLTEDEIATILKSTLKGLEYL 137
Query: 354 HKLKILHRSLNCGSVLIDFNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAP 533
H ++ +HR + G++L++ +AKL +F ++ L D K + + W+AP
Sbjct: 138 HFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMA-------KRNTVIGTPFWMAP 190
Query: 534 EVLLQESYGFKADIWSFGVTMLEMMTGRRPYPQLNNVQAAHRIVADRHIEFDRDLYPNSG 713
EV+ + Y ADIWS G+T +EM G+ PY ++ ++A I + F + +
Sbjct: 191 EVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDD 250
Query: 714 FDEILDTCLRSNQNERPNVFDL 779
F + + CL + +R L
Sbjct: 251 FTDFVKKCLVKSPEQRATATQL 272
>sp|Q13188|STK3_HUMAN Serine/threonine-protein kinase 3 (STE20-like kinase MST2) (MST-2)
(Mammalian STE20-like protein kinase 2)
(Serine/threonine-protein kinase Krs-1)
Length = 491
Score = 91.3 bits (225), Expect = 3e-18
Identities = 56/202 (27%), Positives = 98/202 (48%), Gaps = 1/202 (0%)
Frame = +3
Query: 177 DTSRFVQLLSMYEEENSVFIVMERM-ASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYM 353
D+ V+ Y + ++IVME A S+ +++ + L ED+I L GL Y+
Sbjct: 78 DSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYL 137
Query: 354 HKLKILHRSLNCGSVLIDFNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAP 533
H ++ +HR + G++L++ +AKL +F ++ L D K + + W+AP
Sbjct: 138 HFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMA-------KRNTVIGTPFWMAP 190
Query: 534 EVLLQESYGFKADIWSFGVTMLEMMTGRRPYPQLNNVQAAHRIVADRHIEFDRDLYPNSG 713
EV+ + Y ADIWS G+T +EM G+ PY ++ ++A I + F + +
Sbjct: 191 EVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDD 250
Query: 714 FDEILDTCLRSNQNERPNVFDL 779
F + + CL N +R L
Sbjct: 251 FTDFVKKCLVKNPEQRATATQL 272
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 89.7 bits (221), Expect = 8e-18
Identities = 62/198 (31%), Positives = 96/198 (48%), Gaps = 2/198 (1%)
Frame = +3
Query: 192 VQLLSMYEEENSVFIVMERMASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKIL 371
V+ L E +S+ I+ME + S L E I Y+ ++L GL Y+H I+
Sbjct: 134 VRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYTKQLLLGLEYLHNNGIM 193
Query: 372 HRSLNCGSVLIDFNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAPEVLLQE 551
HR + ++L+D +L +F SK + + +T K +KG+ W+APEV+LQ
Sbjct: 194 HRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTPY-----WMAPEVILQT 248
Query: 552 SYGFKADIWSFGVTMLEMMTGRRPYPQLNNVQAA--HRIVADRHIEFDRDLYPNSGFDEI 725
+ F ADIWS G T++EM TG+ P+ + AA H H DL P + +
Sbjct: 249 GHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKAHPPIPEDLSPEA--KDF 306
Query: 726 LDTCLRSNQNERPNVFDL 779
L CL + R + +L
Sbjct: 307 LMKCLHKEPSLRLSATEL 324
>sp|P50528|PLO1_SCHPO Serine/threonine-protein kinase plo1
Length = 683
Score = 89.4 bits (220), Expect = 1e-17
Identities = 58/230 (25%), Positives = 121/230 (52%), Gaps = 5/230 (2%)
Frame = +3
Query: 105 ISRTNVKNFKMLKYEMVTRFKLPYDTSR--FVQLLSMYEEENSVFIVMERMA-SSLRSLL 275
I++ +++N K K ++ K+ S V + +E+ ++++++E SL LL
Sbjct: 71 IAKRSLQNDKT-KLKLFGEIKVHQSMSHPNIVGFIDCFEDSTNIYLILELCEHKSLMELL 129
Query: 276 KPDTQHLTEDKIKKYSYEILDGLSYMHKLKILHRSLNCGSVLIDFNDNAKLCNFHLSKFL 455
+ Q LTE +++ +IL L YMHK +++HR L G++++D ++N K+ +F L+ L
Sbjct: 130 RKRKQ-LTEPEVRYLMMQILGALKYMHKKRVIHRDLKLGNIMLDESNNVKIGDFGLAALL 188
Query: 456 FDCSTEQLLKGVKGSKIEDDECWVAPEVLL--QESYGFKADIWSFGVTMLEMMTGRRPYP 629
D ++ I ++APE+L +E + F+ D+WS GV M ++ G+ P+
Sbjct: 189 MDDEERKM-------TICGTPNYIAPEILFNSKEGHSFEVDLWSAGVVMYALLIGKPPF- 240
Query: 630 QLNNVQAAHRIVADRHIEFDRDLYPNSGFDEILDTCLRSNQNERPNVFDL 779
Q V+ +R + F ++ ++ +++ + L + + RP++ D+
Sbjct: 241 QDKEVKTIYRKIKANSYSFPSNVDISAEAKDLISSLLTHDPSIRPSIDDI 290
>sp|P38692|KIC1_YEAST Serine/threonine-protein kinase KIC1 (Kinase that interacts with
CDC31) (N-rich kinase 1)
Length = 1080
Score = 87.4 bits (215), Expect = 4e-17
Identities = 61/201 (30%), Positives = 97/201 (48%), Gaps = 2/201 (0%)
Frame = +3
Query: 183 SRFVQLLSMYEEENSVFIVMERMAS-SLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHK 359
S + Y ++ S++I+ME A SLRSLL+P + E I E+L L +HK
Sbjct: 80 SNITRYYGSYLKDTSLWIIMEHCAGGSLRSLLRPGK--IDEKYIGVIMRELLVALKCIHK 137
Query: 360 LKILHRSLNCGSVLIDFNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAPEV 539
++HR + +VLI N KLC+F ++ + S ++ + W+APEV
Sbjct: 138 DNVIHRDIKAANVLITNEGNVKLCDFGVAAQVNQTS-------LRRQTMAGTPYWMAPEV 190
Query: 540 LLQESY-GFKADIWSFGVTMLEMMTGRRPYPQLNNVQAAHRIVADRHIEFDRDLYPNSGF 716
+++ Y K DIWS G+T E+ TG PY + ++A I+ + + Y S
Sbjct: 191 IMEGVYYDTKVDIWSLGITTYEIATGNPPYCDVEALRAMQLIIKSKPPRLEDRSYSTS-L 249
Query: 717 DEILDTCLRSNQNERPNVFDL 779
E + CL + ER + DL
Sbjct: 250 KEFIALCLDEDPKERLSADDL 270
>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 87.4 bits (215), Expect = 4e-17
Identities = 69/208 (33%), Positives = 107/208 (51%), Gaps = 7/208 (3%)
Frame = +3
Query: 60 DLDTPPETC---VIFQYDISRTNVKNFKMLKYEMVTRFKLPYDTSRFVQLLSMYEE--EN 224
D+DT E V F D T+ K L+ E+ L ++ R VQ + E
Sbjct: 381 DVDTGRELASKQVQFDPDSPETS-KEVSALECEIQLLKNLQHE--RIVQYYGCLRDRAEK 437
Query: 225 SVFIVMERM-ASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKILHRSLNCGSVL 401
++ I ME M S++ LK LTE +KY+ +IL+G+SY+H I+HR + ++L
Sbjct: 438 TLTIFMEYMPGGSVKDQLKAYGA-LTESVTRKYTRQILEGMSYLHSNMIVHRDIKGANIL 496
Query: 402 IDFNDNAKLCNFHLSKFLFD-CSTEQLLKGVKGSKIEDDECWVAPEVLLQESYGFKADIW 578
D N KL +F SK L C + ++ V G+ W++PEV+ E YG KAD+W
Sbjct: 497 RDSAGNVKLGDFGASKRLQTICMSGTGMRSVTGTPY-----WMSPEVISGEGYGRKADVW 551
Query: 579 SFGVTMLEMMTGRRPYPQLNNVQAAHRI 662
S G T++EM+T + P+ + + A +I
Sbjct: 552 SLGCTVVEMLTEKPPWAEYEAMAAIFKI 579
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 97,801,564
Number of Sequences: 369166
Number of extensions: 2056009
Number of successful extensions: 9018
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 7574
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7949
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7405750800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)