Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_010_H19
(351 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P26624|GST28_SCHJA Glutathione S-transferase 28 kDa (GST... 105 4e-23
sp|P09792|GST28_SCHMA Glutathione S-transferase 28 kDa (GST... 96 3e-20
sp|P30114|GST28_SCHHA Glutathione S-transferase 28 kDa (GST... 95 6e-20
sp|P30113|GST28_SCHBO Glutathione S-transferase 28 kDa (GST... 91 6e-19
sp|O16116|GST3_CAEEL Glutathione S-transferase 3 (GST class... 79 4e-15
sp|P46088|GST_OMMSL Glutathione S-transferase (GST class-si... 78 5e-15
sp|P46429|GST2_MANSE Glutathione S-transferase 2 (GST class... 76 2e-14
sp|P91253|GST7_CAEEL Probable glutathione S-transferase 7 (... 74 1e-13
sp|O16115|GST2_CAEEL Glutathione S-transferase 2 (GST class... 73 2e-13
sp|Q21355|GST4_CAEEL Glutathione S-transferase 4 (GST class... 72 3e-13
>sp|P26624|GST28_SCHJA Glutathione S-transferase 28 kDa (GST 28) (SJ28 antigen) (GST
class-mu)
Length = 206
Score = 105 bits (261), Expect = 4e-23
Identities = 53/117 (45%), Positives = 77/117 (65%), Gaps = 5/117 (4%)
Frame = +3
Query: 15 HARGRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEGKK-----LTQS 179
+ RGRAE IR++L A+ VEFED R+ + W +KPT P G+LP+V+ K+ +++S
Sbjct: 7 NGRGRAEPIRMILVAAGVEFEDERIEFQDWPKIKPTIPGGRLPIVKITDKRGDVKTMSES 66
Query: 180 MAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFAPEEKKEDLRTEFV 350
+AIAR IARK+ MG+ D EYY+IE+ IGQ+ DV +K P E+KE + E +
Sbjct: 67 LAIARFIARKHNMMGDTDDEYYIIEKMIGQVEDVESEYHKTLIKPPEEKEKISKEIL 123
>sp|P09792|GST28_SCHMA Glutathione S-transferase 28 kDa (GST 28) (SM28 antigen)
(Protective 28 kDa antigen) (GST class-mu)
Length = 211
Score = 95.9 bits (237), Expect = 3e-20
Identities = 49/115 (42%), Positives = 73/115 (63%), Gaps = 5/115 (4%)
Frame = +3
Query: 21 RGRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEG-----KKLTQSMA 185
RGRAE IR+ L A+ V++ED R++ + W +KPT P G+LP V+ K + +S+A
Sbjct: 14 RGRAESIRMTLVAAGVDYEDERISFQDWPKIKPTIPGGRLPAVKVTDDHGHVKWMLESLA 73
Query: 186 IARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFAPEEKKEDLRTEFV 350
IAR +A+K+ MGE D+EYY +E+ IGQ DV +K P+E+KE + E +
Sbjct: 74 IARYMAKKHHMMGETDEEYYSVEKLIGQAEDVEHEYHKTLMKPQEEKEKITKEIL 128
>sp|P30114|GST28_SCHHA Glutathione S-transferase 28 kDa (GST 28) (GST class-mu)
Length = 211
Score = 94.7 bits (234), Expect = 6e-20
Identities = 48/117 (41%), Positives = 74/117 (63%), Gaps = 5/117 (4%)
Frame = +3
Query: 15 HARGRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEG-----KKLTQS 179
+ RGRAE IR+ L A+ V +ED R++ + W +KPT P G+LP V+ K + +S
Sbjct: 12 NGRGRAESIRMTLVAAGVNYEDERISFQDWPKIKPTIPGGRLPAVKITDNHGHVKWMLES 71
Query: 180 MAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFAPEEKKEDLRTEFV 350
+AIAR +A+K+ MGE D+EYY +E+ IGQ+ D+ +K PEE+K+ + E +
Sbjct: 72 LAIARYMAKKHHMMGETDEEYYNVEKLIGQVEDLEHEYHKTLMKPEEEKQKITKEIL 128
>sp|P30113|GST28_SCHBO Glutathione S-transferase 28 kDa (GST 28) (GST class-mu)
Length = 211
Score = 91.3 bits (225), Expect = 6e-19
Identities = 47/117 (40%), Positives = 72/117 (61%), Gaps = 5/117 (4%)
Frame = +3
Query: 15 HARGRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEG-----KKLTQS 179
+ RGRAE IR+ L A+ V +ED R++ + W +KPT P G+LP V+ K + +S
Sbjct: 12 NGRGRAESIRMTLVAAGVNYEDERISFQDWPKIKPTIPGGRLPAVKITDNHGHVKWMVES 71
Query: 180 MAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFAPEEKKEDLRTEFV 350
+AIAR +A+K+ MG ++EYY +E+ IGQ D+ YK PEE+K+ + E +
Sbjct: 72 LAIARYMAKKHHMMGGTEEEYYNVEKLIGQAEDLEHEYYKTLMKPEEEKQKIIKEIL 128
>sp|O16116|GST3_CAEEL Glutathione S-transferase 3 (GST class-sigma) (CeGST3)
Length = 207
Score = 78.6 bits (192), Expect = 4e-15
Identities = 42/117 (35%), Positives = 70/117 (59%), Gaps = 5/117 (4%)
Frame = +3
Query: 15 HARGRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEGKKLTQSMAIAR 194
+ARG AEI R + + VEFED R+ +E++ +KPT P+G++P++ +G + +QS AIAR
Sbjct: 10 NARGLAEISRQLFHMAGVEFEDERINEEKFSQLKPTFPSGQVPILCIDGAQFSQSTAIAR 69
Query: 195 AIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFA-----PEEKKEDLRTEFV 350
+ARK+GF+G+ +E + + D +E K A EE +++R E +
Sbjct: 70 YLARKFGFVGQTAEEELQADEVVDTFKDFIESFRKFVIAVLSGESEEILKNIREEVI 126
>sp|P46088|GST_OMMSL Glutathione S-transferase (GST class-sigma)
Length = 203
Score = 78.2 bits (191), Expect = 5e-15
Identities = 40/114 (35%), Positives = 63/114 (55%)
Frame = +3
Query: 6 HLLHARGRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEGKKLTQSMA 185
H GRAE+ R VL A EF D + W ++K T + +PV++ +G K++QSM
Sbjct: 7 HYFPLMGRAELCRFVLAAHGEEFTDRVVEMADWPNLKATMYSNAMPVLDIDGTKMSQSMC 66
Query: 186 IARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFAPEEKKEDLRTEF 347
IAR +AR++G G+ E Y ++ + D+ + KI FAPE KE ++ +
Sbjct: 67 IARHLAREFGLDGKTSLEKYRVDEITETLQDIFNDVVKIKFAPEAAKEAVQQNY 120
>sp|P46429|GST2_MANSE Glutathione S-transferase 2 (GST class-sigma)
Length = 203
Score = 76.3 bits (186), Expect = 2e-14
Identities = 39/113 (34%), Positives = 65/113 (57%)
Frame = +3
Query: 6 HLLHARGRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEGKKLTQSMA 185
H A+G A L+ + EFED R+ EQW + KP TP G++PV+E +GKK QS+A
Sbjct: 7 HYFGAKGWARPTMLLAYGGQ-EFEDHRVEYEQWPEFKPNTPFGQMPVLEIDGKKYAQSLA 65
Query: 186 IARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFAPEEKKEDLRTE 344
I+R + RKYG G D +E + I++ + + D+ + + + ++++ E
Sbjct: 66 ISRYLGRKYGLAGNDIEEDFEIDQIVDFVNDIRASAASVEYEQDAANKEVKHE 118
>sp|P91253|GST7_CAEEL Probable glutathione S-transferase 7 (GST class-sigma)
Length = 206
Score = 73.9 bits (180), Expect = 1e-13
Identities = 39/88 (44%), Positives = 54/88 (61%)
Frame = +3
Query: 21 RGRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEGKKLTQSMAIARAI 200
RG EI R +L + +FED R+ KE+W VKP+TP G+LP++E +GK L QS AIAR +
Sbjct: 12 RGAGEIARQILAYAGQDFEDNRIPKEEWPAVKPSTPFGQLPLLEVDGKVLAQSHAIARYL 71
Query: 201 ARKYGFMGEDDKEYYLIERAIGQMVDVL 284
AR++G G+ E + Q D L
Sbjct: 72 ARQFGINGKCAWEEAQVNSVADQFKDYL 99
>sp|O16115|GST2_CAEEL Glutathione S-transferase 2 (GST class-sigma) (CeGST2)
Length = 188
Score = 73.2 bits (178), Expect = 2e-13
Identities = 38/115 (33%), Positives = 66/115 (57%), Gaps = 5/115 (4%)
Frame = +3
Query: 21 RGRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEGKKLTQSMAIARAI 200
RG E+IR + V FED R+++E++ +K P+G+LPV+E +G ++QS +I R +
Sbjct: 12 RGLGEVIRQLFYLGDVSFEDFRVSREEFKSLKSNLPSGQLPVLEIDGVMISQSASIGRFL 71
Query: 201 ARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFA-----PEEKKEDLRTEFV 350
AR+YG+ G+ E ++ I D + + +FA PE +KE ++ + V
Sbjct: 72 ARQYGYSGKTPTEEMQVDSIIDLFKDFMLTFRQFFFAVIHGYPEYEKERMKRDIV 126
>sp|Q21355|GST4_CAEEL Glutathione S-transferase 4 (GST class-sigma) (CeGST1)
Length = 207
Score = 72.4 bits (176), Expect = 3e-13
Identities = 34/89 (38%), Positives = 56/89 (62%)
Frame = +3
Query: 18 ARGRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEGKKLTQSMAIARA 197
AR AE IR++ V +ED R++ E+W +KPTTP G+LP+++ +G++ QSM+I R
Sbjct: 11 ARALAEPIRIMFAMLNVPYEDYRVSVEEWSKLKPTTPFGQLPILQVDGEQFGQSMSITRY 70
Query: 198 IARKYGFMGEDDKEYYLIERAIGQMVDVL 284
+ARK+G G+ +E + + Q D +
Sbjct: 71 LARKFGLAGKTAEEEAYADSIVDQYRDFI 99
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 41,643,347
Number of Sequences: 369166
Number of extensions: 833621
Number of successful extensions: 2721
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2641
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2676
length of database: 68,354,980
effective HSP length: 84
effective length of database: 52,837,240
effective search space used: 1690791680
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)