Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_010_H07
(238 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9NI63|PMYT1_DROME Membrane-associated tyrosine-and thre... 33 0.27
sp|P47810|WEE1_MOUSE Wee1-like protein kinase 32 0.46
sp|Q10407|MKH1_SCHPO MAP kinase kinase kinase mkh1 32 0.46
sp|Q63802|WEE1_RAT Wee1-like protein kinase 30 1.7
sp|P54936|LIN2_CAEEL Abnormal cell lineage protein 2 (Prote... 30 2.3
sp|P30291|WEE1_HUMAN Wee1-like protein kinase (WEE1hu) 30 2.3
sp|Q8VCR8|MYLK2_MOUSE Myosin light chain kinase 2, skeletal... 29 3.9
sp|Q9DBQ7|PACE1_MOUSE Protein-associating with the carboxyl... 28 5.1
sp|P20689|MYLK2_RAT Myosin light chain kinase 2, skeletal/c... 28 6.6
sp|O14733|MP2K7_HUMAN Dual specificity mitogen-activated pr... 28 6.6
>sp|Q9NI63|PMYT1_DROME Membrane-associated tyrosine-and threonine-specific cdc2-inhibitory
kinase (Myt1 kinase) (dMyt1)
Length = 533
Score = 32.7 bits (73), Expect = 0.27
Identities = 13/29 (44%), Positives = 22/29 (75%)
Frame = +2
Query: 62 ISETMQSFIRALLNPDQSRRPRADNLLNH 148
IS +QS I++++ PD ++RP A+ LL+H
Sbjct: 320 ISLELQSVIKSMMKPDPAQRPTAEQLLSH 348
>sp|P47810|WEE1_MOUSE Wee1-like protein kinase
Length = 646
Score = 32.0 bits (71), Expect = 0.46
Identities = 12/33 (36%), Positives = 21/33 (63%)
Frame = +2
Query: 56 QPISETMQSFIRALLNPDQSRRPRADNLLNHSI 154
Q +S+ + +R +++PD RRP A L+ HS+
Sbjct: 535 QVLSQEVTELLRVMIHPDPERRPSAMELVKHSV 567
>sp|Q10407|MKH1_SCHPO MAP kinase kinase kinase mkh1
Length = 1116
Score = 32.0 bits (71), Expect = 0.46
Identities = 20/63 (31%), Positives = 30/63 (47%), Gaps = 3/63 (4%)
Frame = +2
Query: 2 VAVMNKIVTTKKIECTPCQPISETMQS---FIRALLNPDQSRRPRADNLLNHSIFNNCDE 172
+ M K+ T KK P + +S+ F+ A + RP A+ LLNH F CDE
Sbjct: 1040 IQAMFKLGTEKKAPPIPSELVSQVSPEAIQFLNACFTVNADVRPTAEELLNHP-FMKCDE 1098
Query: 173 FYS 181
++
Sbjct: 1099 EFN 1101
>sp|Q63802|WEE1_RAT Wee1-like protein kinase
Length = 646
Score = 30.0 bits (66), Expect = 1.7
Identities = 11/33 (33%), Positives = 21/33 (63%)
Frame = +2
Query: 56 QPISETMQSFIRALLNPDQSRRPRADNLLNHSI 154
Q +S+ + ++ +++PD RRP A L+ HS+
Sbjct: 535 QVLSQELTELLKVMIHPDPERRPSAMVLVKHSV 567
>sp|P54936|LIN2_CAEEL Abnormal cell lineage protein 2 (Protein lin-2)
Length = 961
Score = 29.6 bits (65), Expect = 2.3
Identities = 13/29 (44%), Positives = 19/29 (65%)
Frame = +2
Query: 62 ISETMQSFIRALLNPDQSRRPRADNLLNH 148
ISE+ ++ +R +LN D S+R A LNH
Sbjct: 250 ISESARNLVRRMLNADPSKRISAKEALNH 278
>sp|P30291|WEE1_HUMAN Wee1-like protein kinase (WEE1hu)
Length = 646
Score = 29.6 bits (65), Expect = 2.3
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = +2
Query: 56 QPISETMQSFIRALLNPDQSRRPRADNLLNHSI 154
Q +S+ ++ +++PD RRP A L+ HS+
Sbjct: 536 QVLSQEFTELLKVMIHPDPERRPSAMALVKHSV 568
>sp|Q8VCR8|MYLK2_MOUSE Myosin light chain kinase 2, skeletal/cardiac muscle (MLCK2)
Length = 240
Score = 28.9 bits (63), Expect = 3.9
Identities = 14/39 (35%), Positives = 20/39 (51%)
Frame = +2
Query: 56 QPISETMQSFIRALLNPDQSRRPRADNLLNHSIFNNCDE 172
+ +S+ + F+ LL DQS R A+ L H NN E
Sbjct: 151 EAVSDEAKDFVSNLLTKDQSARMSAEQCLAHPWLNNLAE 189
>sp|Q9DBQ7|PACE1_MOUSE Protein-associating with the carboxyl-terminal domain of ezrin
(Ezrin-binding protein PACE-1) (SCY1-like protein 3)
Length = 735
Score = 28.5 bits (62), Expect = 5.1
Identities = 14/34 (41%), Positives = 17/34 (50%)
Frame = +2
Query: 62 ISETMQSFIRALLNPDQSRRPRADNLLNHSIFNN 163
+S +Q ALLNP RP LL+H F N
Sbjct: 214 LSSFLQILHSALLNPMPECRPALSTLLSHDFFRN 247
>sp|P20689|MYLK2_RAT Myosin light chain kinase 2, skeletal/cardiac muscle (MLCK2)
Length = 610
Score = 28.1 bits (61), Expect = 6.6
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = +2
Query: 56 QPISETMQSFIRALLNPDQSRRPRADNLLNHSIFNNCDE 172
+ +S+ + F+ L+ DQS R A+ L H NN E
Sbjct: 521 EAVSDEAKDFVSNLITKDQSARMSAEQCLAHPWLNNLAE 559
>sp|O14733|MP2K7_HUMAN Dual specificity mitogen-activated protein kinase kinase 7 (MAP
kinase kinase 7) (MAPKK 7) (MAPK/ERK kinase 7)
(JNK-activating kinase 2) (c-Jun N-terminal kinase
kinase 2) (JNK kinase 2) (JNKK 2)
Length = 419
Score = 28.1 bits (61), Expect = 6.6
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +2
Query: 65 SETMQSFIRALLNPDQSRRPRADNLLNHS 151
S QSF++ L D +RP+ + LL HS
Sbjct: 350 SGDFQSFVKDCLTKDHRKRPKYNKLLEHS 378
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,133,997
Number of Sequences: 369166
Number of extensions: 306590
Number of successful extensions: 752
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 749
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 752
length of database: 68,354,980
effective HSP length: 49
effective length of database: 59,302,965
effective search space used: 1719785985
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)