Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_010_F22
(526 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q07192|MP2K2_XENLA Dual specificity mitogen-activated pr... 144 1e-34
sp|P47809|MP2K4_MOUSE Dual specificity mitogen-activated pr... 144 2e-34
sp|P45985|MP2K4_HUMAN Dual specificity mitogen-activated pr... 144 2e-34
sp|P70236|MP2K6_MOUSE Dual specificity mitogen-activated pr... 142 5e-34
sp|O09110|MP2K3_MOUSE Dual specificity mitogen-activated pr... 142 5e-34
sp|P52564|MP2K6_HUMAN Dual specificity mitogen-activated pr... 140 1e-33
sp|P46734|MP2K3_HUMAN Dual specificity mitogen-activated pr... 140 1e-33
sp|Q20347|YR62_CAEEL Putative serine/threonine-protein kina... 127 1e-29
sp|P08018|PBS2_YEAST Polymyxin B resistance protein kinase 121 9e-28
sp|P33886|WIS1_SCHPO Protein kinase wis1 (Protein kinase sty2) 119 3e-27
>sp|Q07192|MP2K2_XENLA Dual specificity mitogen-activated protein kinase kinase 2 (MAP
kinase kinase 2) (MAPKK 2) (MAPK-ERK kinase 2)
Length = 446
Score = 144 bits (363), Expect = 1e-34
Identities = 69/132 (52%), Positives = 95/132 (71%), Gaps = 3/132 (2%)
Frame = +1
Query: 1 DVKPSNTLIDKGGRVKVCDFGISGDLINSMAKT-NVGSQPYLAPERINPDRSGAGYSIRS 177
D+KPSN L+D G +K+CDFGISG L++S+AKT + G +PY+APERI+P S GY +RS
Sbjct: 276 DIKPSNILLDTNGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPSASRQGYDVRS 335
Query: 178 DVWSLGLSVLELAIRKFPYSRWNSLFDQLKDVVASDTPPAL--PADSVYSDTVRNFISQC 351
DVWSLG+++ ELA +FPY +WNS+FDQL VV D PP L + +S + +F++QC
Sbjct: 336 DVWSLGITLYELATGRFPYPKWNSVFDQLTQVVKGD-PPQLSNSEEREFSPSFTSFVNQC 394
Query: 352 LVKKDSDRASYE 387
L K +S R Y+
Sbjct: 395 LTKDESKRPKYK 406
>sp|P47809|MP2K4_MOUSE Dual specificity mitogen-activated protein kinase kinase 4 (MAP
kinase kinase 4) (MAPKK 4) (MAPK/ERK kinase 4)
(JNK-activating kinase 1) (C-JUN N-terminal kinase
kinase 1) (JNK kinase 1) (JNKK 1) (SAPK/ERK kinase 1)
(SEK1)
Length = 397
Score = 144 bits (362), Expect = 2e-34
Identities = 69/132 (52%), Positives = 95/132 (71%), Gaps = 3/132 (2%)
Frame = +1
Query: 1 DVKPSNTLIDKGGRVKVCDFGISGDLINSMAKT-NVGSQPYLAPERINPDRSGAGYSIRS 177
D+KPSN L+D+ G +K+CDFGISG L++S+AKT + G +PY+APERI+P S GY +RS
Sbjct: 227 DIKPSNILLDRSGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPSASRQGYDVRS 286
Query: 178 DVWSLGLSVLELAIRKFPYSRWNSLFDQLKDVVASDTPPAL--PADSVYSDTVRNFISQC 351
DVWSLG+++ ELA +FPY +WNS+FDQL VV D PP L + +S + NF++ C
Sbjct: 287 DVWSLGITLYELATGRFPYPKWNSVFDQLTQVVKGD-PPQLSNSEEREFSPSFINFVNLC 345
Query: 352 LVKKDSDRASYE 387
L K +S R Y+
Sbjct: 346 LTKDESKRPKYK 357
>sp|P45985|MP2K4_HUMAN Dual specificity mitogen-activated protein kinase kinase 4 (MAP
kinase kinase 4) (JNK-activating kinase 1) (c-Jun
N-terminal kinase kinase 1) (JNKK) (SAPK/ERK kinase 1)
(SEK1)
Length = 399
Score = 144 bits (362), Expect = 2e-34
Identities = 69/132 (52%), Positives = 95/132 (71%), Gaps = 3/132 (2%)
Frame = +1
Query: 1 DVKPSNTLIDKGGRVKVCDFGISGDLINSMAKT-NVGSQPYLAPERINPDRSGAGYSIRS 177
D+KPSN L+D+ G +K+CDFGISG L++S+AKT + G +PY+APERI+P S GY +RS
Sbjct: 229 DIKPSNILLDRSGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPSASRQGYDVRS 288
Query: 178 DVWSLGLSVLELAIRKFPYSRWNSLFDQLKDVVASDTPPAL--PADSVYSDTVRNFISQC 351
DVWSLG+++ ELA +FPY +WNS+FDQL VV D PP L + +S + NF++ C
Sbjct: 289 DVWSLGITLYELATGRFPYPKWNSVFDQLTQVVKGD-PPQLSNSEEREFSPSFINFVNLC 347
Query: 352 LVKKDSDRASYE 387
L K +S R Y+
Sbjct: 348 LTKDESKRPKYK 359
>sp|P70236|MP2K6_MOUSE Dual specificity mitogen-activated protein kinase kinase 6 (MAP
kinase kinase 6) (MAPKK 6) (MAPK/ERK kinase 6) (SAPKK3)
Length = 334
Score = 142 bits (358), Expect = 5e-34
Identities = 71/129 (55%), Positives = 96/129 (74%), Gaps = 1/129 (0%)
Frame = +1
Query: 1 DVKPSNTLIDKGGRVKVCDFGISGDLINSMAKT-NVGSQPYLAPERINPDRSGAGYSIRS 177
DVKPSN LI+ G+VK+CDFGISG L++S+AKT + G +PY+APERINP+ + GYS++S
Sbjct: 179 DVKPSNVLINTLGQVKMCDFGISGYLVDSVAKTIDAGCKPYMAPERINPELNQKGYSVKS 238
Query: 178 DVWSLGLSVLELAIRKFPYSRWNSLFDQLKDVVASDTPPALPADSVYSDTVRNFISQCLV 357
D+WSLG++++ELAI +FPY W + F QLK VV + P LPAD +D V +F SQCL
Sbjct: 239 DIWSLGITMIELAILRFPYDSWGTPFQQLKQVV-EEPSPQLPADKFSADFV-DFTSQCLK 296
Query: 358 KKDSDRASY 384
K +R +Y
Sbjct: 297 KNSKERPTY 305
>sp|O09110|MP2K3_MOUSE Dual specificity mitogen-activated protein kinase kinase 3 (MAP
kinase kinase 3) (MAPKK 3) (MAPK/ERK kinase 3)
Length = 347
Score = 142 bits (358), Expect = 5e-34
Identities = 71/129 (55%), Positives = 96/129 (74%), Gaps = 1/129 (0%)
Frame = +1
Query: 1 DVKPSNTLIDKGGRVKVCDFGISGDLINSMAKT-NVGSQPYLAPERINPDRSGAGYSIRS 177
DVKPSN LI+K G VK+CDFGISG L++S+AKT + G +PY+APERINP+ + GY+++S
Sbjct: 190 DVKPSNVLINKEGHVKMCDFGISGYLVDSVAKTMDAGCKPYMAPERINPELNQKGYNVKS 249
Query: 178 DVWSLGLSVLELAIRKFPYSRWNSLFDQLKDVVASDTPPALPADSVYSDTVRNFISQCLV 357
DVWSLG++++E+AI +FPY W + F QLK VV + P LPAD +S +F SQCL
Sbjct: 250 DVWSLGITMIEMAILRFPYESWGTPFQQLKQVV-EEPSPQLPADQ-FSPEFVDFTSQCLR 307
Query: 358 KKDSDRASY 384
K ++R SY
Sbjct: 308 KNPAERMSY 316
>sp|P52564|MP2K6_HUMAN Dual specificity mitogen-activated protein kinase kinase 6 (MAP
kinase kinase 6) (MAPKK 6) (MAPK/ERK kinase 6) (SAPKK3)
Length = 334
Score = 140 bits (354), Expect = 1e-33
Identities = 70/129 (54%), Positives = 96/129 (74%), Gaps = 1/129 (0%)
Frame = +1
Query: 1 DVKPSNTLIDKGGRVKVCDFGISGDLINSMAKT-NVGSQPYLAPERINPDRSGAGYSIRS 177
DVKPSN LI+ G+VK+CDFGISG L++S+AKT + G +PY+APERINP+ + GYS++S
Sbjct: 179 DVKPSNVLINALGQVKMCDFGISGYLVDSVAKTIDAGCKPYMAPERINPELNQKGYSVKS 238
Query: 178 DVWSLGLSVLELAIRKFPYSRWNSLFDQLKDVVASDTPPALPADSVYSDTVRNFISQCLV 357
D+WSLG++++ELAI +FPY W + F QLK VV + P LPAD ++ V +F SQCL
Sbjct: 239 DIWSLGITMIELAILRFPYDSWGTPFQQLKQVV-EEPSPQLPADKFSAEFV-DFTSQCLK 296
Query: 358 KKDSDRASY 384
K +R +Y
Sbjct: 297 KNSKERPTY 305
>sp|P46734|MP2K3_HUMAN Dual specificity mitogen-activated protein kinase kinase 3 (MAP
kinase kinase 3) (MAPKK 3) (MAPK/ERK kinase 3)
Length = 347
Score = 140 bits (354), Expect = 1e-33
Identities = 70/129 (54%), Positives = 96/129 (74%), Gaps = 1/129 (0%)
Frame = +1
Query: 1 DVKPSNTLIDKGGRVKVCDFGISGDLINSMAKT-NVGSQPYLAPERINPDRSGAGYSIRS 177
DVKPSN LI+K G VK+CDFGISG L++S+AKT + G +PY+APERINP+ + GY+++S
Sbjct: 190 DVKPSNVLINKEGHVKMCDFGISGYLVDSVAKTMDAGCKPYMAPERINPELNQKGYNVKS 249
Query: 178 DVWSLGLSVLELAIRKFPYSRWNSLFDQLKDVVASDTPPALPADSVYSDTVRNFISQCLV 357
DVWSLG++++E+AI +FPY W + F QLK VV + P LPAD +S +F +QCL
Sbjct: 250 DVWSLGITMIEMAILRFPYESWGTPFQQLKQVV-EEPSPQLPADR-FSPEFVDFTAQCLR 307
Query: 358 KKDSDRASY 384
K ++R SY
Sbjct: 308 KNPAERMSY 316
>sp|Q20347|YR62_CAEEL Putative serine/threonine-protein kinase F42G10.2
Length = 363
Score = 127 bits (320), Expect = 1e-29
Identities = 72/133 (54%), Positives = 85/133 (63%), Gaps = 4/133 (3%)
Frame = +1
Query: 1 DVKPSNTLIDKGGRVKVCDFGISGDLINSMAKTN-VGSQPYLAPERINPDRSGAGYSIRS 177
DVKPSN L+D G VK+CDFGI G L S AKT+ G QPYLAPERI S Y +RS
Sbjct: 194 DVKPSNILVDGTGAVKLCDFGICGQLEESFAKTHDAGCQPYLAPERIT---SSDKYDVRS 250
Query: 178 DVWSLGLSVLELAIRKFPYSRWNSLFDQLKDVVASDTPPALPADS---VYSDTVRNFISQ 348
DVWSLG+++ E+A KFPY WNSLFDQ+ VV+ D PP L DS YS + FI+
Sbjct: 251 DVWSLGITLYEIATGKFPYQEWNSLFDQIATVVSGD-PPILHPDSDDFHYSLPLVKFINT 309
Query: 349 CLVKKDSDRASYE 387
CL K R Y+
Sbjct: 310 CLTKDRRHRPKYD 322
>sp|P08018|PBS2_YEAST Polymyxin B resistance protein kinase
Length = 668
Score = 121 bits (304), Expect = 9e-28
Identities = 66/134 (49%), Positives = 93/134 (69%), Gaps = 6/134 (4%)
Frame = +1
Query: 1 DVKPSNTLIDKG-GRVKVCDFGISGDLINSMAKTNVGSQPYLAPERI---NPDRSGAGYS 168
DVKP+N L G VK+CDFG+SG+L+ S+AKTN+G Q Y+APERI NPDR A Y+
Sbjct: 485 DVKPTNILCSANQGTVKLCDFGVSGNLVASLAKTNIGCQSYMAPERIKSLNPDR--ATYT 542
Query: 169 IRSDVWSLGLSVLELAIRKFPY--SRWNSLFDQLKDVVASDTPPALPADSVYSDTVRNFI 342
++SD+WSLGLS+LE+A+ ++PY ++++F QL +V PP LP+D SD ++F+
Sbjct: 543 VQSDIWSLGLSILEMALGRYPYPPETYDNIFSQLSAIV-DGPPPRLPSDKFSSD-AQDFV 600
Query: 343 SQCLVKKDSDRASY 384
S CL K R +Y
Sbjct: 601 SLCLQKIPERRPTY 614
>sp|P33886|WIS1_SCHPO Protein kinase wis1 (Protein kinase sty2)
Length = 605
Score = 119 bits (299), Expect = 3e-27
Identities = 62/133 (46%), Positives = 92/133 (69%), Gaps = 5/133 (3%)
Frame = +1
Query: 1 DVKPSNTLIDKGGRVKVCDFGISGDLINSMAKTNVGSQPYLAPERI---NPDRSGAGYSI 171
DVKP+N L++ G+VK+CDFG+SG+L+ S++KTN+G Q Y+APERI P Y++
Sbjct: 441 DVKPTNVLVNSNGQVKLCDFGVSGNLVASISKTNIGCQSYMAPERIRVGGPTNGVLTYTV 500
Query: 172 RSDVWSLGLSVLELAIRKFPY--SRWNSLFDQLKDVVASDTPPALPADSVYSDTVRNFIS 345
++DVWSLGL++LE+A+ +PY + S+F QL + D PP+LP DS +S R+F++
Sbjct: 501 QADVWSLGLTILEMALGAYPYPPESYTSIFAQLSAICDGD-PPSLP-DS-FSPEARDFVN 557
Query: 346 QCLVKKDSDRASY 384
+CL K S R Y
Sbjct: 558 KCLNKNPSLRPDY 570
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 59,309,417
Number of Sequences: 369166
Number of extensions: 1170612
Number of successful extensions: 5426
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4583
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4742
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3502236525
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)