Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_010_F13
(980 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Con... 86 1e-16
sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Con... 82 2e-15
sp|O13035|SAP_CHICK Proactivator polypeptide precursor [Con... 68 5e-11
sp|Q61207|SAP_MOUSE Sulfated glycoprotein 1 precursor (SGP-... 64 7e-10
sp|P10960|SAP_RAT Sulfated glycoprotein 1 precursor (SGP-1)... 58 4e-08
sp|P20097|SAP_CAVPO Saposin C (CO-beta-glucosidase) (Glucos... 41 0.006
sp|P50405|PSPB_MOUSE Pulmonary surfactant-associated protei... 40 0.013
sp|P17129|PSPB_CANFA Pulmonary surfactant-associated protei... 39 0.022
sp|O04057|ASPR_CUCPE Aspartic proteinase precursor 38 0.038
sp|Q89330|POLG_ZYMVR Genome polyprotein [Contains: P1 prote... 37 0.085
>sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B-Val; Saposin B (Sphingolipid activator
protein 1) (SAP-1) (Cerebroside sulfate activator)
(CSAct) (Dispersin) (Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 524
Score = 86.3 bits (212), Expect = 1e-16
Identities = 58/217 (26%), Positives = 101/217 (46%), Gaps = 5/217 (2%)
Frame = +1
Query: 283 CEICRFAILEVRTLLTDPGFQK-IFENFLINEICPHF-KTKQQQCTNDINQYYSLVVNII 456
CE+C F + EV L+ + +K I + F +++C K+ ++C ++ Y S +++I+
Sbjct: 315 CEVCEFLVKEVTKLIDNNKTEKEILDAF--DKMCSKLPKSLSEECQEVVDTYGSSILSIL 372
Query: 457 VEFLNPSL-CSELGLCNSTIKQVIMRQPLVQINRSKKTSNCRLCCKFYNYL-QTISSKSS 630
+E ++P L CS L LC+ T + V + + K C +C K YL + + S+
Sbjct: 373 LEEVSPELVCSMLHLCSGTRLPALT----VHVTQPKDGGFCEVCKKLVGYLDRNLEKNST 428
Query: 631 HSNLLFGLVSFCNQISAHERLECLKQFRNFNAVIAAFNKSKTINEF-CEMTKMCSHQRKN 807
+L L C+ + + +C + + V+ F C C K
Sbjct: 429 KQEILAALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHKP 488
Query: 808 PLGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 918
LG C WGP +WC + + A C AV+HC+ +VW
Sbjct: 489 LLGTEKCIWGPSYWCQNTETAAQC--NAVEHCKRHVW 523
Score = 63.9 bits (154), Expect = 7e-10
Identities = 49/181 (27%), Positives = 93/181 (51%), Gaps = 6/181 (3%)
Frame = +1
Query: 1 ELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL-SVIKNECVIIVDTYLPVIFSIARS 177
E+CE ++ EV K++ +N +E+ + + K+C +L + EC +VDTY I SI
Sbjct: 316 EVCEFLVKEVTKLIDNNKTEKEILDAFD-KMCSKLPKSLSEECQEVVDTYGSSILSILLE 374
Query: 178 TLNSKVLCTELKLC--TLLSKKKISFLMNRSGNETLKCEICRFAILEV-RTLLTDPGFQK 348
++ +++C+ L LC T L + + G CE+C+ + + R L + Q+
Sbjct: 375 EVSPELVCSMLHLCSGTRLPALTVHVTQPKDGG---FCEVCKKLVGYLDRNLEKNSTKQE 431
Query: 349 IFENFLINEICPHFKTK-QQQCTNDINQYYSLVVNIIVEFLNPS-LCSELGLCNSTIKQV 522
I + + C Q+QC + +Y +++ I+VE ++PS +C ++G C S K +
Sbjct: 432 ILA--ALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHKPL 489
Query: 523 I 525
+
Sbjct: 490 L 490
Score = 41.6 bits (96), Expect = 0.003
Identities = 64/333 (19%), Positives = 128/333 (38%), Gaps = 66/333 (19%)
Frame = +1
Query: 1 ELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRLSV--IKNECVIIVDTYLPVIFSIAR 174
++C+ V+ ++ N +EE + +++ K C L + C IVD+YLPVI I +
Sbjct: 64 DICKDVVTAAGDMLKDNATEEEILVYLE-KTCDWLPKPNMSASCKEIVDSYLPVILDIIK 122
Query: 175 STLNSK-VLCTELKLCTLLSKKKISFLMNRSGNETLK----------------------- 282
++ +C+ L LC L +K ++ L ++ E+ K
Sbjct: 123 GEMSRPGEVCSALNLCESL-QKHLAELNHQKQLESNKIPELDMTEVVAPFMANIPLLLYP 181
Query: 283 ----------------CEICRFAILEVRTLL-TDPGF-QKIFENFLINEICPHFKTKQQQ 408
C+ C + +++T + T+ F Q + E+ + E C
Sbjct: 182 QDGPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTFVQALVEH--VKEECDRLGPGMAD 239
Query: 409 -CTNDINQYYSLVVNIIVEFLNPSLCSELGLCNS--------------TIKQVIMRQPLV 543
C N I+QY + + +++ +C+ +G C+ K VI LV
Sbjct: 240 ICKNYISQYSEIAIQMMMHMQPKEICALVGFCDEVKEMPMQTLVPAKVASKNVIPALELV 299
Query: 544 Q-INRSKKTSNCRLCCKFYNYL-----QTISSKSSHSNLLFGLVSFCNQISAHERLECLK 705
+ I + + + + C+ +L + I + + +L C+++ EC +
Sbjct: 300 EPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKSLSEECQE 359
Query: 706 QFRNFNAVIAAFNKSKTINEF-CEMTKMCSHQR 801
+ + I + + E C M +CS R
Sbjct: 360 VVDTYGSSILSILLEEVSPELVCSMLHLCSGTR 392
Score = 37.7 bits (86), Expect = 0.050
Identities = 18/38 (47%), Positives = 20/38 (52%)
Frame = +1
Query: 811 LGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVWRQ 924
LG CT G WC + K A C GAVKHC VW +
Sbjct: 20 LGLKECTRGSAVWCQNVKTASDC--GAVKHCLQTVWNK 55
>sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B (Sphingolipid activator protein 1) (SAP-1)
(Cerebroside sulfate activator) (CSAct) (Dispersin)
(Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 525
Score = 82.0 bits (201), Expect = 2e-15
Identities = 56/216 (25%), Positives = 96/216 (44%), Gaps = 4/216 (1%)
Frame = +1
Query: 283 CEICRFAILEVRTLLTDPGFQKIFENFLINEICPHFKTK-QQQCTNDINQYYSLVVNIIV 459
CE+C F + EV L+ + ++ + L +++C T +QC ++ Y +++I++
Sbjct: 316 CEVCEFVVKEVAKLIDNNRTEEEILHAL-DKVCSKLPTSLAEQCQEVVDTYGRSILSILL 374
Query: 460 EFLNPSL-CSELGLCNSTIKQVIMRQPLVQINRSKKTSNCRLCCKFYNYL-QTISSKSSH 633
+ +P L CS L LC+S + V++ K C +C K YL + + S+
Sbjct: 375 DEASPELVCSMLHLCSSRG----LPAATVRVMPRKDGGFCEVCKKLVGYLDRNLEKNSTK 430
Query: 634 SNLLFGLVSFCNQISAHERLECLKQFRNFNAVIAAFNKSKTINEF-CEMTKMCSHQRKNP 810
+L L C+ + R +C + + V+ F C C K
Sbjct: 431 EQILAALEKGCSFLPDQYRKQCDQFVTEYEPVLIEILVEVMDPSFVCLKIGACPAAHKPL 490
Query: 811 LGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 918
LG C WGP +WC + + A C AV+HC +VW
Sbjct: 491 LGAEKCVWGPSYWCQNMESAALC--NAVEHCRRHVW 524
Score = 63.9 bits (154), Expect = 7e-10
Identities = 46/181 (25%), Positives = 96/181 (53%), Gaps = 6/181 (3%)
Frame = +1
Query: 1 ELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL-SVIKNECVIIVDTYLPVIFSIARS 177
E+CE V+ EV K++ +N +EE + + + K+C +L + + +C +VDTY I SI
Sbjct: 317 EVCEFVVKEVAKLIDNNRTEEEILHALD-KVCSKLPTSLAEQCQEVVDTYGRSILSILLD 375
Query: 178 TLNSKVLCTELKLCTL--LSKKKISFLMNRSGNETLKCEICRFAILEV-RTLLTDPGFQK 348
+ +++C+ L LC+ L + + + G CE+C+ + + R L + ++
Sbjct: 376 EASPELVCSMLHLCSSRGLPAATVRVMPRKDGG---FCEVCKKLVGYLDRNLEKNSTKEQ 432
Query: 349 IFENFLINEICPHFKTK-QQQCTNDINQYYSLVVNIIVEFLNPS-LCSELGLCNSTIKQV 522
I + + C + ++QC + +Y +++ I+VE ++PS +C ++G C + K +
Sbjct: 433 ILA--ALEKGCSFLPDQYRKQCDQFVTEYEPVLIEILVEVMDPSFVCLKIGACPAAHKPL 490
Query: 523 I 525
+
Sbjct: 491 L 491
Score = 37.0 bits (84), Expect = 0.085
Identities = 58/328 (17%), Positives = 115/328 (35%), Gaps = 64/328 (19%)
Frame = +1
Query: 1 ELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRLSV--IKNECVIIVDTYLPVIFSIAR 174
++C+ V+ ++ N +E+ + +++ + C L + C IVD+YLPVI + +
Sbjct: 64 DICKDVITAAGNLLKDNATEQEILMYLE-RTCDWLPKPNMSASCKEIVDSYLPVILDMIK 122
Query: 175 STLNSK-VLCTELKLCTLLSK-----------------------------KKISFLMNRS 264
++ +C+ L LC L K I FL+
Sbjct: 123 GQMSHPGEVCSALNLCESLQKHLAELNHQKQLESNQIPELDMAEVVAPFMANIPFLLYPQ 182
Query: 265 GNETLK----------CEICRFAILEVR-TLLTDPGFQKIFENFLINEICPHFKTKQQQC 411
K C+ C + +V+ L T+ F + + E C
Sbjct: 183 DGSHSKPQPKKANGNVCQDCIQLVTDVQEALRTNSTFVEALVDHAKEECDRLGPGMSDMC 242
Query: 412 TNDINQYYSLVVNIIVEFLNPSLCSELGLCNS--------------TIKQVIMRQPLVQ- 546
N INQY + + +++ +C G C+ + VI LV+
Sbjct: 243 KNYINQYSEVAIQMVMHMQPKEICVLAGFCDEVKEMPMKTLVPAEVVSENVIPALGLVEP 302
Query: 547 INRSKKTSNCRLCCKFYNYL-----QTISSKSSHSNLLFGLVSFCNQISAHERLECLKQF 711
I + + + C+ ++ + I + + +L L C+++ +C +
Sbjct: 303 IKKDPAPAKADIYCEVCEFVVKEVAKLIDNNRTEEEILHALDKVCSKLPTSLAEQCQEVV 362
Query: 712 RNFNAVIAAFNKSKTINEF-CEMTKMCS 792
+ I + + E C M +CS
Sbjct: 363 DTYGRSILSILLDEASPELVCSMLHLCS 390
Score = 36.6 bits (83), Expect = 0.11
Identities = 17/36 (47%), Positives = 19/36 (52%)
Frame = +1
Query: 811 LGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 918
LG CT G WC + K A C GAV+HC VW
Sbjct: 20 LGLRECTRGSAVWCQNVKTAADC--GAVQHCLQTVW 53
>sp|O13035|SAP_CHICK Proactivator polypeptide precursor [Contains: Saposin A; Saposin B;
Saposin C; Saposin D]
Length = 518
Score = 67.8 bits (164), Expect = 5e-11
Identities = 68/311 (21%), Positives = 130/311 (41%), Gaps = 11/311 (3%)
Frame = +1
Query: 19 MDEVIKVVTSNHSEEVVSNFIKLKICPRLSVIKNECVIIVDTYLPVIFSIARSTLNSKVL 198
M ++ K S +S+ + + +K K+ C ++ + P + S+ TL +
Sbjct: 236 MSDMCKSYISEYSDLAIQMMMHMKD----QQPKDICAMV--GFCPSVKSVPLQTLVPAQV 289
Query: 199 CTELKLCTL----LSKKKISFLMNRSGNETLKCEICRFAILEVRTLLTDPGFQK--IFEN 360
E+K+ T+ + +K S CEIC + EV LL ++ + E
Sbjct: 290 VHEVKMETVEKATVQEKTFSV-----------CEICETMVKEVTGLLESNKTEEEIVHEM 338
Query: 361 FLINEICPHFKTKQQQCTNDINQYYSLVVNIIVEFLNP-SLCSELGLC--NSTIKQVIMR 531
++ + P + + QC + I Y ++++++E NP ++C L C N +Q ++
Sbjct: 339 EVVCYLLP--ASVKDQCKDFIEVYGQALIDMLLEATNPEAVCVMLKCCAANKPPQQPVVV 396
Query: 532 QPLVQINRSKKTSNCRLCCKFYNYLQTISSKSSHSNLLFGLVS-FCNQISAHERLECLKQ 708
+P C +C Y K++ + + L+ C+ + +C++
Sbjct: 397 KPAGGF--------CDICKMIVAYADKELEKNATTTEIEALLEKVCHFLPESVSDQCVQF 448
Query: 709 FRNFNAVIAAFNKSKTINEF-CEMTKMCSHQRKNPLGKNPCTWGPGFWCVSRKHAVACGP 885
+ V+ F C +C +K LG++ C WGPG+WC + + A C
Sbjct: 449 VEQYEPVVVQLLAEMMDPTFVCTKLGVCGAAKKPLLGEDACVWGPGYWCKNMETAAQC-- 506
Query: 886 GAVKHCEMNVW 918
AV HC +VW
Sbjct: 507 NAVDHCRRHVW 517
Score = 63.5 bits (153), Expect = 9e-10
Identities = 40/178 (22%), Positives = 87/178 (48%), Gaps = 3/178 (1%)
Frame = +1
Query: 1 ELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRLSVIKNECVIIVDTYLPVIFSIARST 180
E+CE ++ EV ++ SN +EE + + +++ + +K++C ++ Y + +
Sbjct: 312 EICETMVKEVTGLLESNKTEEEIVHEMEVVCYLLPASVKDQCKDFIEVYGQALIDMLLEA 371
Query: 181 LNSKVLCTELKLCTLLSKKKISFLMNRSGNETLKCEICRFAILEVRTLLTDPGFQKIFEN 360
N + +C LK C + ++ +G C+IC+ + L E
Sbjct: 372 TNPEAVCVMLKCCAANKPPQQPVVVKPAGG---FCDICKMIVAYADKELEKNATTTEIE- 427
Query: 361 FLINEICPHF--KTKQQQCTNDINQYYSLVVNIIVEFLNPS-LCSELGLCNSTIKQVI 525
L+ ++C HF ++ QC + QY +VV ++ E ++P+ +C++LG+C + K ++
Sbjct: 428 ALLEKVC-HFLPESVSDQCVQFVEQYEPVVVQLLAEMMDPTFVCTKLGVCGAAKKPLL 484
Score = 45.4 bits (106), Expect = 2e-04
Identities = 27/81 (33%), Positives = 48/81 (59%), Gaps = 3/81 (3%)
Frame = +1
Query: 1 ELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL--SVIKNECVIIVDTYLPVIFSIAR 174
+LC+ ++ V KV+ N +E+ + ++++ K C L + +EC IVD+YLPVI + +
Sbjct: 65 DLCKELVTVVGKVLKDNGTEDEIRSYLE-KRCEFLPDQGLASECKEIVDSYLPVIMDMIK 123
Query: 175 STLNS-KVLCTELKLCTLLSK 234
+ +V+C+ L LC L K
Sbjct: 124 EEFDKPEVVCSALSLCQSLQK 144
Score = 40.8 bits (94), Expect = 0.006
Identities = 18/36 (50%), Positives = 21/36 (58%)
Frame = +1
Query: 811 LGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 918
L + C GP WC S + A CG AVKHC+ NVW
Sbjct: 21 LWQKDCAKGPEVWCQSLRTASQCG--AVKHCQQNVW 54
>sp|Q61207|SAP_MOUSE Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)
Length = 557
Score = 63.9 bits (154), Expect = 7e-10
Identities = 49/247 (19%), Positives = 94/247 (38%), Gaps = 30/247 (12%)
Frame = +1
Query: 268 NETLKCEICRFAILEVRTLLTDPGFQKIFENFLINEICPHFKTKQQQCTNDINQYYSLVV 447
+ + C+ C+F + + L+ + +++ L N + +C + + ++
Sbjct: 312 HNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLL 371
Query: 448 NIIVEFLNPS-LCSELGLCNS-------------TIKQVIMRQPL--------------V 543
+I + +NPS LC +GLC + I ++++P
Sbjct: 372 DIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPA 431
Query: 544 QINRSKKTSNCRLCCKFYNYLQ-TISSKSSHSNLLFGLVSFCNQISAHERLECLKQFRNF 720
+ K C +C K YL+ + S+ +L L C+ + + +C +
Sbjct: 432 HVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEY 491
Query: 721 NAVIAAFNKSKTINEF-CEMTKMCSHQRKNPLGKNPCTWGPGFWCVSRKHAVACGPGAVK 897
++ F C +C K LG C WGP +WC + + A C AV
Sbjct: 492 EPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARC--NAVD 549
Query: 898 HCEMNVW 918
HC+ +VW
Sbjct: 550 HCKRHVW 556
Score = 41.2 bits (95), Expect = 0.005
Identities = 33/131 (25%), Positives = 62/131 (47%), Gaps = 6/131 (4%)
Frame = +1
Query: 187 SKVLCTELKLCTLLSKKKI--SFLMNRSGNETLKCEICRFAILEVRTLLTDPGFQKIFEN 360
S VLC ++K K + ++ ++L C+IC+ + E LL D Q+ +
Sbjct: 29 SAVLCRDVKTAVDCGAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATQEEILH 88
Query: 361 FLINEICP--HFKTKQQQCTNDINQYYSLVVNIIV-EFLNP-SLCSELGLCNSTIKQVIM 528
+L + C H + C ++ Y +++++I E NP +CS L LC S +++ +
Sbjct: 89 YL-EKTCEWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQS-LQEYLA 146
Query: 529 RQPLVQINRSK 561
Q Q+ +K
Sbjct: 147 EQNQKQLESNK 157
Score = 41.2 bits (95), Expect = 0.005
Identities = 67/333 (20%), Positives = 133/333 (39%), Gaps = 69/333 (20%)
Frame = +1
Query: 1 ELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL--SVIKNECVIIVDTYLPVIFSIAR 174
++C+ V+ E ++ N ++E + ++++ K C + S + C +VD+YLPVI + +
Sbjct: 64 DICKTVVTEAGNLLKDNATQEEILHYLE-KTCEWIHDSSLSASCKEVVDSYLPVILDMIK 122
Query: 175 STL-NSKVLCTELKLCTLL-------SKKKIS---------------FLMN--------- 258
+ N +C+ L LC L ++K++ F+ N
Sbjct: 123 GEMSNPGEVCSALNLCQSLQEYLAEQNQKQLESNKIPEVDMARVVAPFMSNIPLLLYPQD 182
Query: 259 -------RSGNETLKCEICRFAILEVRTLL-TDPGFQKIFENFLINEICPHFKTKQQQ-C 411
NE + C+ C + +V+T + T+ F + F + + E C C
Sbjct: 183 HPRSQPQPKANEDV-CQDCMKLVSDVQTAVKTNSSFIQGFVDH-VKEDCDRLGPGVSDIC 240
Query: 412 TNDINQYYSLVVNIIVEFLN---PSLCSELGLCN--------------STIKQVI----M 528
N ++QY + V +++ + +C G CN TIK ++ M
Sbjct: 241 KNYVDQYSEVCVQMLMHMQDQQPKEICVLAGFCNEVKRVPMKTLVPATETIKNILPALEM 300
Query: 529 RQPLVQINRSKKTSNCRLC--CKFY--NYLQTISSKSSHSNLLFGLVSFCNQISAHERLE 696
P Q + N LC C+F + + I + ++ L+ GL + C + R +
Sbjct: 301 MDPYEQ--NLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTK 358
Query: 697 CLKQFRNFN-AVIAAFNKSKTINEFCEMTKMCS 792
C + F +++ F + C + +C+
Sbjct: 359 CQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCA 391
Score = 31.2 bits (69), Expect = 4.7
Identities = 15/31 (48%), Positives = 17/31 (54%)
Frame = +1
Query: 826 CTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 918
C+ G C K AV CG AVKHC+ VW
Sbjct: 25 CSGGSAVLCRDVKTAVDCG--AVKHCQQMVW 53
>sp|P10960|SAP_RAT Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)
Length = 554
Score = 58.2 bits (139), Expect = 4e-08
Identities = 47/242 (19%), Positives = 93/242 (38%), Gaps = 30/242 (12%)
Frame = +1
Query: 283 CEICRFAILEVRTLLTDPGFQKIFENFLINEICPHFKTKQQQCTNDINQYYSLVVNIIVE 462
C++C+ + ++ L+ + +++ L +C + + ++++++
Sbjct: 314 CQVCQLVMRKLSELIINNATEELLIKGLSKACSLLPAPASTKCQEVLVTFGPSLLDVLMH 373
Query: 463 FLNPS-LCSELGLCNSTIKQV-IMRQPLVQINRS-------------------------- 558
+NP+ LC + LC++ V + QP I +
Sbjct: 374 EVNPNFLCGVISLCSANPNLVGTLEQPAAAIVSALPKEPAPPKQPEEPKQSALRAHVPPQ 433
Query: 559 KKTSNCRLCCKFYNYLQ-TISSKSSHSNLLFGLVSFCNQISAHERLECLKQFRNFNAVIA 735
K C +C K YL+ + S+ +L L C+ + + +C + + ++
Sbjct: 434 KNGGFCEVCKKLVIYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDEFVAEYEPLLL 493
Query: 736 AFNKSKTINEF-CEMTKMCSHQRKNPLGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMN 912
F C +C K LG C WGPG+WC + + A C AV HC+ +
Sbjct: 494 EILVEVMDPSFVCSKIGVCPSAYKLLLGTEKCVWGPGYWCQNSETAARC--NAVDHCKRH 551
Query: 913 VW 918
VW
Sbjct: 552 VW 553
Score = 45.1 bits (105), Expect = 3e-04
Identities = 65/330 (19%), Positives = 133/330 (40%), Gaps = 66/330 (20%)
Frame = +1
Query: 1 ELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL--SVIKNECVIIVDTYLPVIFSIAR 174
++C+ V+ E ++ N +EE + ++++ K C + S + C +VD+YLPVI + +
Sbjct: 64 DICKTVVTEAGNLLKDNATEEEILHYLE-KTCAWIHDSSLSASCKEVVDSYLPVILDMIK 122
Query: 175 STL-NSKVLCTELKLCTLLSK------------KKI----------SFLMN--------- 258
+ N +C+ L LC L + KI F+ N
Sbjct: 123 GEMSNPGEVCSALNLCQSLQEYLAEQNQRQLESNKIPEVDLARVVAPFMSNIPLLLYPQD 182
Query: 259 -------RSGNETLKCEICRFAILEVRTLL-TDPGF-QKIFENFLINEICPHFKTKQQQ- 408
NE + C+ C + +++T + T+ F Q + ++ + E C
Sbjct: 183 RPRSQPQPKANEDV-CQDCMKLVTDIQTAVRTNSSFVQGLVDH--VKEDCDRLGPGVSDI 239
Query: 409 CTNDINQYYSLVVNIIVEFLNPSLCSELGLCNSTIKQVIMRQ------------PLVQIN 552
C N ++QY + V +++ +C +G C+ +K+V MR P +++
Sbjct: 240 CKNYVDQYSEVAVQMMMHMQPKEICVMVGFCDE-VKRVPMRTLVPATEAIKNILPALELT 298
Query: 553 --------RSKKTSNCRLCCKFYNYL-QTISSKSSHSNLLFGLVSFCNQISAHERLECLK 705
+++ C++C L + I + ++ L+ GL C+ + A +C +
Sbjct: 299 DPYEQDVIQAQNVIFCQVCQLVMRKLSELIINNATEELLIKGLSKACSLLPAPASTKCQE 358
Query: 706 QFRNFN-AVIAAFNKSKTINEFCEMTKMCS 792
F +++ N C + +CS
Sbjct: 359 VLVTFGPSLLDVLMHEVNPNFLCGVISLCS 388
Score = 38.1 bits (87), Expect = 0.038
Identities = 31/131 (23%), Positives = 62/131 (47%), Gaps = 6/131 (4%)
Frame = +1
Query: 187 SKVLCTELKLCTLLSKKKI--SFLMNRSGNETLKCEICRFAILEVRTLLTDPGFQKIFEN 360
S V+C ++K K + ++ ++L C+IC+ + E LL D ++ +
Sbjct: 29 SAVVCRDVKTAVDCRAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATEEEILH 88
Query: 361 FLINEICP--HFKTKQQQCTNDINQYYSLVVNIIV-EFLNP-SLCSELGLCNSTIKQVIM 528
+L + C H + C ++ Y +++++I E NP +CS L LC S +++ +
Sbjct: 89 YL-EKTCAWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQS-LQEYLA 146
Query: 529 RQPLVQINRSK 561
Q Q+ +K
Sbjct: 147 EQNQRQLESNK 157
>sp|P20097|SAP_CAVPO Saposin C (CO-beta-glucosidase) (Glucosylceramidase activator)
(Sphingolipid activator protein 2) (SAP-2)
Length = 81
Score = 40.8 bits (94), Expect = 0.006
Identities = 21/72 (29%), Positives = 44/72 (61%), Gaps = 1/72 (1%)
Frame = +1
Query: 7 CEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRLSVIKNE-CVIIVDTYLPVIFSIARSTL 183
CE+V+ +V++++ +N +EE + + + +C L +E C +VDTY I ++ +
Sbjct: 8 CEYVVKKVMELIDNNRTEEKIIHALD-SVCALLPESVSEVCQEVVDTYGDSIVALLLQEM 66
Query: 184 NSKVLCTELKLC 219
+ +++C+EL LC
Sbjct: 67 SPELVCSELGLC 78
Score = 35.4 bits (80), Expect = 0.25
Identities = 19/80 (23%), Positives = 43/80 (53%), Gaps = 1/80 (1%)
Frame = +1
Query: 271 ETLKCEICRFAILEVRTLLTDPGFQKIFENFLINEICPHFKTKQQQCTNDINQYYSLVVN 450
E++ C+ C + + +V L+ + ++ + L + ++ + C ++ Y +V
Sbjct: 1 ESVTCKACEYVVKKVMELIDNNRTEEKIIHALDSVCALLPESVSEVCQEVVDTYGDSIVA 60
Query: 451 IIVEFLNPSL-CSELGLCNS 507
++++ ++P L CSELGLC S
Sbjct: 61 LLLQEMSPELVCSELGLCMS 80
>sp|P50405|PSPB_MOUSE Pulmonary surfactant-associated protein B precursor (SP-B)
(Pulmonary surfactant-associated proteolipid SPL(Phe))
Length = 377
Score = 39.7 bits (91), Expect = 0.013
Identities = 19/73 (26%), Positives = 39/73 (53%), Gaps = 2/73 (2%)
Frame = +1
Query: 7 CEHVMDEVIKVVTSNHSEEVVSNFIKLK--ICPRLSVIKNECVIIVDTYLPVIFSIARST 180
CE ++ + K+ + +E + F++ + I P L ++ C ++D YLP++ +S
Sbjct: 71 CEDIVHLLTKMTKEDAFQEAIRKFLEQECDILP-LKLLVPRCRQVLDVYLPLVIDYFQSQ 129
Query: 181 LNSKVLCTELKLC 219
+N K +C + LC
Sbjct: 130 INPKAICNHVGLC 142
Score = 37.7 bits (86), Expect = 0.050
Identities = 16/31 (51%), Positives = 18/31 (58%)
Frame = +1
Query: 826 CTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 918
C GP FWC S +HAV C A+ HC VW
Sbjct: 31 CAQGPQFWCQSLEHAVQC--RALGHCLQEVW 59
>sp|P17129|PSPB_CANFA Pulmonary surfactant-associated protein B precursor (SP-B) (6 kDa
protein) (Pulmonary surfactant-associated proteolipid
SPL(Phe)) (Pulmonary surfactant protein 18) (SP 18)
Length = 363
Score = 38.9 bits (89), Expect = 0.022
Identities = 19/79 (24%), Positives = 41/79 (51%), Gaps = 6/79 (7%)
Frame = +1
Query: 1 ELCEHVMDEVIKVVTSNHSEEVVSNFIK------LKICPRLSVIKNECVIIVDTYLPVIF 162
+LC+ D +++++T E + + ++ + P L ++ +C ++ TY PV+
Sbjct: 60 DLCQECQD-IVRILTKMTKEAIFQDMVRKFLEHECDVLP-LKLLTPQCHHMLGTYFPVVV 117
Query: 163 SIARSTLNSKVLCTELKLC 219
+S +N K++C L LC
Sbjct: 118 DYFQSQINPKIICKHLGLC 136
Score = 33.9 bits (76), Expect = 0.72
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = +1
Query: 826 CTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 918
C GP FWC S + A+ C A+ HC VW
Sbjct: 25 CARGPAFWCQSLEQALQC--RALGHCLQEVW 53
Score = 33.9 bits (76), Expect = 0.72
Identities = 21/76 (27%), Positives = 37/76 (48%), Gaps = 3/76 (3%)
Frame = +1
Query: 283 CEICRFAILEVRTLLTDPGFQKIFENFLINE--ICPHFKTKQQQCTNDINQYYSLVVNII 456
C+ C+ + + + + FQ + FL +E + P K QC + + Y+ +VV+
Sbjct: 62 CQECQDIVRILTKMTKEAIFQDMVRKFLEHECDVLP-LKLLTPQCHHMLGTYFPVVVDYF 120
Query: 457 VEFLNPS-LCSELGLC 501
+NP +C LGLC
Sbjct: 121 QSQINPKIICKHLGLC 136
>sp|O04057|ASPR_CUCPE Aspartic proteinase precursor
Length = 513
Score = 38.1 bits (87), Expect = 0.038
Identities = 25/121 (20%), Positives = 49/121 (40%), Gaps = 10/121 (8%)
Frame = +1
Query: 109 VIKNECVIIVDTYLPVIFSIARSTLNSKVLCTELKLCTLLSKKKISFLMNRSGNETL--- 279
V+ +C +V Y I + S + K +C+++ LCT + +S + +E
Sbjct: 319 VVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDGTRGVSMGIESVVDENAGKS 378
Query: 280 -------KCEICRFAILEVRTLLTDPGFQKIFENFLINEICPHFKTKQQQCTNDINQYYS 438
C +C ++ ++ L ++ N+ INE+C + Q D Q S
Sbjct: 379 SDSLHDGMCSVCEMTVVWMQNQLRQNQTKERIINY-INELCDRMPSPMGQSAVDCGQLSS 437
Query: 439 L 441
+
Sbjct: 438 M 438
>sp|Q89330|POLG_ZYMVR Genome polyprotein [Contains: P1 proteinase (N-terminal protein);
Helper component proteinase (HC-pro); Protein P3; 6 kDa
protein 1 (6K1); Cytoplasmic inclusion protein (CI); 6
kDa protein 2 (6K2); Viral genome-linked protein (VPg);
Nuclear inclusion protein A (NI-a) (NIa) (NIa-pro) (49
kDa proteinase) (49 kDa-Pro); Nuclear inclusion protein B
(NI-b) (NIb) (RNA-directed RNA polymerase); Coat protein
(CP)]
Length = 3083
Score = 37.0 bits (84), Expect = 0.085
Identities = 20/48 (41%), Positives = 26/48 (54%), Gaps = 2/48 (4%)
Frame = -3
Query: 363 KIFKNFLKSRISQQCSDFKNSKSAYFTLQCFITTSIHKK--RDFLFRK 226
K F F + +++QQ D K S F +CFITT +H K RD RK
Sbjct: 948 KRFSIFTERKLTQQAKDGKRVSSLQFVHECFITTRVHAKSIRDVGMRK 995
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 110,067,954
Number of Sequences: 369166
Number of extensions: 2309129
Number of successful extensions: 6298
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5967
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6285
length of database: 68,354,980
effective HSP length: 111
effective length of database: 47,849,395
effective search space used: 10287619925
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)