Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_010_F05
(585 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q91X78|SPFH1_MOUSE SPFH domain protein 1 precursor (KE04... 138 1e-32
sp|O75477|SPFH1_HUMAN SPFH domain protein 1 precursor (Prot... 136 3e-32
sp|Q5RCJ9|SPFH1_PONPY SPFH domain protein 1 precursor 136 3e-32
sp|O94905|SPFH2_HUMAN SPFH domain protein 2 precursor >gi|6... 135 1e-31
sp|Q8BFZ9|SPFH2_MOUSE SPFH domain protein 2 precursor 134 2e-31
sp|P08799|MYS2_DICDI Myosin II heavy chain, non muscle 39 0.007
sp|O13127|FLOT1_CARAU Flotillin-1 (Reggie-2) (REG-2) 35 0.11
sp|P23607|ZFA_MOUSE Zinc finger autosomal protein 34 0.23
sp|O61491|FLOT1_DROME Flotillin-1 34 0.31
sp|P26038|MOES_HUMAN Moesin (Membrane-organizing extension ... 33 0.40
>sp|Q91X78|SPFH1_MOUSE SPFH domain protein 1 precursor (KE04 protein homolog)
Length = 346
Score = 138 bits (347), Expect = 1e-32
Identities = 72/130 (55%), Positives = 102/130 (78%), Gaps = 5/130 (3%)
Frame = +2
Query: 2 IASERQRVVEKEAETERLRAIIEAEKLAQVSRIHWQMKITEKESELNISRIEDAARTSRI 181
IA+++Q+VVEKEAETER RA+IEAEK+AQV++I +Q K+ EKE+E IS IEDAA +R
Sbjct: 195 IAAQKQKVVEKEAETERKRAVIEAEKIAQVAKIRFQQKVMEKETEKRISEIEDAAFLARE 254
Query: 182 KSESDAEFYRAKQEAEANKVKLTQEYLEMIRLLSIANNTKIYFGNSIPQMFLDSS----- 346
K+++DAE+Y A + A +NK KLT EYLE+ + +IA+N+KIYFG++IP MF+DSS
Sbjct: 255 KAKADAEYYAAHKYATSNKHKLTPEYLELKKYQAIASNSKIYFGSNIPSMFVDSSCALKY 314
Query: 347 NSGQTNQDYS 376
+ G+T ++ S
Sbjct: 315 SDGRTGREDS 324
>sp|O75477|SPFH1_HUMAN SPFH domain protein 1 precursor (Protein KE04)
Length = 346
Score = 136 bits (343), Expect = 3e-32
Identities = 68/115 (59%), Positives = 95/115 (82%)
Frame = +2
Query: 2 IASERQRVVEKEAETERLRAIIEAEKLAQVSRIHWQMKITEKESELNISRIEDAARTSRI 181
IA+++Q+VVEKEAETER +A+IEAEK+AQV++I +Q K+ EKE+E IS IEDAA +R
Sbjct: 195 IAAQKQKVVEKEAETERKKAVIEAEKIAQVAKIRFQQKVMEKETEKRISEIEDAAFLARE 254
Query: 182 KSESDAEFYRAKQEAEANKVKLTQEYLEMIRLLSIANNTKIYFGNSIPQMFLDSS 346
K+++DAE+Y A + A +NK KLT EYLE+ + +IA+N+KIYFG++IP MF+DSS
Sbjct: 255 KAKADAEYYAAHKYATSNKHKLTPEYLELKKYQAIASNSKIYFGSNIPNMFVDSS 309
>sp|Q5RCJ9|SPFH1_PONPY SPFH domain protein 1 precursor
Length = 346
Score = 136 bits (343), Expect = 3e-32
Identities = 68/115 (59%), Positives = 95/115 (82%)
Frame = +2
Query: 2 IASERQRVVEKEAETERLRAIIEAEKLAQVSRIHWQMKITEKESELNISRIEDAARTSRI 181
IA+++Q+VVEKEAETER +A+IEAEK+AQV++I +Q K+ EKE+E IS IEDAA +R
Sbjct: 195 IAAQKQKVVEKEAETERKKAVIEAEKIAQVAKIRFQQKVMEKETEKRISEIEDAAFLARE 254
Query: 182 KSESDAEFYRAKQEAEANKVKLTQEYLEMIRLLSIANNTKIYFGNSIPQMFLDSS 346
K+++DAE+Y A + A +NK KLT EYLE+ + +IA+N+KIYFG++IP MF+DSS
Sbjct: 255 KAKADAEYYAAHKYATSNKHKLTPEYLELKKYQAIASNSKIYFGSNIPNMFVDSS 309
>sp|O94905|SPFH2_HUMAN SPFH domain protein 2 precursor
sp|Q5R7C5|SPFH2_PONPY SPFH domain protein 2 precursor
Length = 339
Score = 135 bits (339), Expect = 1e-31
Identities = 68/117 (58%), Positives = 94/117 (80%)
Frame = +2
Query: 2 IASERQRVVEKEAETERLRAIIEAEKLAQVSRIHWQMKITEKESELNISRIEDAARTSRI 181
IA+++Q+VVEKEAETER +A+IEAEK+AQV+ I + K+ EKE+E IS IEDAA +R
Sbjct: 195 IAAQKQKVVEKEAETERKKALIEAEKVAQVAEITYGQKVMEKETEKKISEIEDAAFLARE 254
Query: 182 KSESDAEFYRAKQEAEANKVKLTQEYLEMIRLLSIANNTKIYFGNSIPQMFLDSSNS 352
K+++DAE Y A + AEANK+KLT EYL++++ +IA+N+KIYFG IP MF+DS+ S
Sbjct: 255 KAKADAECYTAMKIAEANKLKLTPEYLQLMKYKAIASNSKIYFGKDIPNMFMDSAGS 311
>sp|Q8BFZ9|SPFH2_MOUSE SPFH domain protein 2 precursor
Length = 340
Score = 134 bits (336), Expect = 2e-31
Identities = 72/138 (52%), Positives = 101/138 (73%), Gaps = 1/138 (0%)
Frame = +2
Query: 2 IASERQRVVEKEAETERLRAIIEAEKLAQVSRIHWQMKITEKESELNISRIEDAARTSRI 181
IA+++Q+VVEKEAETER +A+IEAEK+AQV+ I + K+ EKE+E IS IEDAA +R
Sbjct: 195 IAAQKQKVVEKEAETERKKALIEAEKVAQVAEITYGQKVMEKETEKKISEIEDAAFLARE 254
Query: 182 KSESDAEFYRAKQEAEANKVKLTQEYLEMIRLLSIANNTKIYFGNSIPQMFLDSSNS-GQ 358
K+++DAE Y A + AEANK+KLT EYL++++ +IA+N+KIYFG IP MF+DS+ G+
Sbjct: 255 KAKADAECYTALKIAEANKLKLTPEYLQLMKYKAIASNSKIYFGKDIPNMFMDSAGGLGK 314
Query: 359 TNQDYSTSTPGIKLPNLP 412
+ S G L + P
Sbjct: 315 QFEGLSDDKLGFGLEDEP 332
>sp|P08799|MYS2_DICDI Myosin II heavy chain, non muscle
Length = 2116
Score = 39.3 bits (90), Expect = 0.007
Identities = 41/122 (33%), Positives = 53/122 (43%)
Frame = +2
Query: 35 EAETERLRAIIEAEKLAQVSRIHWQMKITEKESELNISRIEDAARTSRIKSESDAEFYRA 214
E E RL+ +EAE+LA+ S + Q K E + +ED KS AE A
Sbjct: 1177 EGEVARLQGELEAEQLAK-SNVEKQKKKVELD-------LED-------KSAQLAEETAA 1221
Query: 215 KQEAEANKVKLTQEYLEMIRLLSIANNTKIYFGNSIPQMFLDSSNSGQTNQDYSTSTPGI 394
KQ + K KL QE E+ LS ANN + NS TN+ TS +
Sbjct: 1222 KQALDKLKKKLEQELSEVQTQLSEANNKNV--------------NSDSTNKHLETSFNNL 1267
Query: 395 KL 400
KL
Sbjct: 1268 KL 1269
>sp|O13127|FLOT1_CARAU Flotillin-1 (Reggie-2) (REG-2)
Length = 423
Score = 35.4 bits (80), Expect = 0.11
Identities = 21/82 (25%), Positives = 43/82 (52%), Gaps = 4/82 (4%)
Frame = +2
Query: 23 VVEKEAETERLRAIIEAEKLAQVSRIHWQMKITEKESELNISRIEDAARTSRIKSE---- 190
V K+AE+E + A+ Q+ Q+ + E+ ++ + E A + ++++
Sbjct: 227 VCTKKAESEMAYQLQVAKTKQQIEEEKMQVMVVERSQQIMLQEQEIARKEKELEAQVMKP 286
Query: 191 SDAEFYRAKQEAEANKVKLTQE 256
+DAE YR ++ AEA +++L E
Sbjct: 287 ADAERYRLEKLAEAERLQLIME 308
>sp|P23607|ZFA_MOUSE Zinc finger autosomal protein
Length = 742
Score = 34.3 bits (77), Expect = 0.23
Identities = 22/77 (28%), Positives = 37/77 (48%), Gaps = 3/77 (3%)
Frame = +2
Query: 23 VVEKEAETERLRAIIEAEKLAQVSR---IHWQMKITEKESELNISRIEDAARTSRIKSES 193
+VE E E E +++ V R ++ + +++E ELN++ I D I E
Sbjct: 215 IVESEPENELGVELLDPNNSICVpreKMVYMAVNNSQQEEELNVTEIADEIYMEVIVGEE 274
Query: 194 DAEFYRAKQEAEANKVK 244
DA +Q+ E NK+K
Sbjct: 275 DATASEHEQQVEDNKMK 291
>sp|O61491|FLOT1_DROME Flotillin-1
Length = 426
Score = 33.9 bits (76), Expect = 0.31
Identities = 26/93 (27%), Positives = 46/93 (49%), Gaps = 3/93 (3%)
Frame = +2
Query: 2 IASERQRVVEKEAETER-LRAIIEAEK--LAQVSRIHWQMKITEKESELNISRIEDAART 172
IA + Q ++ +E E E +R EAEK + +++ + Q + E E+E RI A
Sbjct: 268 IAVQEQEIMRRERELEATIRRPAEAEKFRMEKLAEANKQRVVMEAEAEAESIRIRGEAEA 327
Query: 173 SRIKSESDAEFYRAKQEAEANKVKLTQEYLEMI 271
I +++ AE + +AEA + +EM+
Sbjct: 328 FAIAAKAKAEAEQMAMKAEAYREYREAAMVEML 360
>sp|P26038|MOES_HUMAN Moesin (Membrane-organizing extension spike protein)
Length = 577
Score = 33.5 bits (75), Expect = 0.40
Identities = 36/94 (38%), Positives = 44/94 (46%), Gaps = 14/94 (14%)
Frame = +2
Query: 35 EAETERLRAIIEAEKLAQVSRIHWQMKITEKESELNISR----------IEDAARTSRIK 184
E E ER RA EAEKLA+ Q KE+ L SR +E A T+RI
Sbjct: 374 ELEQERKRAQSEAEKLAK----ERQEAEEAKEALLQASRDQKKTQEQLALEMAELTARI- 428
Query: 185 SESDAEFYRAKQEAEA----NKVKLTQEYLEMIR 274
S E R K+E+EA K ++ QE LE R
Sbjct: 429 --SQLEMARQKKESEAVEWQQKAQMVQEDLEKTR 460
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57,599,430
Number of Sequences: 369166
Number of extensions: 1068549
Number of successful extensions: 3073
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2954
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3060
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4357244645
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)