Planarian EST Database


Dr_sW_010_F05

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_010_F05
         (585 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q91X78|SPFH1_MOUSE  SPFH domain protein 1 precursor (KE04...   138   1e-32
sp|O75477|SPFH1_HUMAN  SPFH domain protein 1 precursor (Prot...   136   3e-32
sp|Q5RCJ9|SPFH1_PONPY  SPFH domain protein 1 precursor            136   3e-32
sp|O94905|SPFH2_HUMAN  SPFH domain protein 2 precursor >gi|6...   135   1e-31
sp|Q8BFZ9|SPFH2_MOUSE  SPFH domain protein 2 precursor            134   2e-31
sp|P08799|MYS2_DICDI  Myosin II heavy chain, non muscle            39   0.007
sp|O13127|FLOT1_CARAU  Flotillin-1 (Reggie-2) (REG-2)              35   0.11 
sp|P23607|ZFA_MOUSE  Zinc finger autosomal protein                 34   0.23 
sp|O61491|FLOT1_DROME  Flotillin-1                                 34   0.31 
sp|P26038|MOES_HUMAN  Moesin (Membrane-organizing extension ...    33   0.40 
>sp|Q91X78|SPFH1_MOUSE SPFH domain protein 1 precursor (KE04 protein homolog)
          Length = 346

 Score =  138 bits (347), Expect = 1e-32
 Identities = 72/130 (55%), Positives = 102/130 (78%), Gaps = 5/130 (3%)
 Frame = +2

Query: 2   IASERQRVVEKEAETERLRAIIEAEKLAQVSRIHWQMKITEKESELNISRIEDAARTSRI 181
           IA+++Q+VVEKEAETER RA+IEAEK+AQV++I +Q K+ EKE+E  IS IEDAA  +R 
Sbjct: 195 IAAQKQKVVEKEAETERKRAVIEAEKIAQVAKIRFQQKVMEKETEKRISEIEDAAFLARE 254

Query: 182 KSESDAEFYRAKQEAEANKVKLTQEYLEMIRLLSIANNTKIYFGNSIPQMFLDSS----- 346
           K+++DAE+Y A + A +NK KLT EYLE+ +  +IA+N+KIYFG++IP MF+DSS     
Sbjct: 255 KAKADAEYYAAHKYATSNKHKLTPEYLELKKYQAIASNSKIYFGSNIPSMFVDSSCALKY 314

Query: 347 NSGQTNQDYS 376
           + G+T ++ S
Sbjct: 315 SDGRTGREDS 324
>sp|O75477|SPFH1_HUMAN SPFH domain protein 1 precursor (Protein KE04)
          Length = 346

 Score =  136 bits (343), Expect = 3e-32
 Identities = 68/115 (59%), Positives = 95/115 (82%)
 Frame = +2

Query: 2   IASERQRVVEKEAETERLRAIIEAEKLAQVSRIHWQMKITEKESELNISRIEDAARTSRI 181
           IA+++Q+VVEKEAETER +A+IEAEK+AQV++I +Q K+ EKE+E  IS IEDAA  +R 
Sbjct: 195 IAAQKQKVVEKEAETERKKAVIEAEKIAQVAKIRFQQKVMEKETEKRISEIEDAAFLARE 254

Query: 182 KSESDAEFYRAKQEAEANKVKLTQEYLEMIRLLSIANNTKIYFGNSIPQMFLDSS 346
           K+++DAE+Y A + A +NK KLT EYLE+ +  +IA+N+KIYFG++IP MF+DSS
Sbjct: 255 KAKADAEYYAAHKYATSNKHKLTPEYLELKKYQAIASNSKIYFGSNIPNMFVDSS 309
>sp|Q5RCJ9|SPFH1_PONPY SPFH domain protein 1 precursor
          Length = 346

 Score =  136 bits (343), Expect = 3e-32
 Identities = 68/115 (59%), Positives = 95/115 (82%)
 Frame = +2

Query: 2   IASERQRVVEKEAETERLRAIIEAEKLAQVSRIHWQMKITEKESELNISRIEDAARTSRI 181
           IA+++Q+VVEKEAETER +A+IEAEK+AQV++I +Q K+ EKE+E  IS IEDAA  +R 
Sbjct: 195 IAAQKQKVVEKEAETERKKAVIEAEKIAQVAKIRFQQKVMEKETEKRISEIEDAAFLARE 254

Query: 182 KSESDAEFYRAKQEAEANKVKLTQEYLEMIRLLSIANNTKIYFGNSIPQMFLDSS 346
           K+++DAE+Y A + A +NK KLT EYLE+ +  +IA+N+KIYFG++IP MF+DSS
Sbjct: 255 KAKADAEYYAAHKYATSNKHKLTPEYLELKKYQAIASNSKIYFGSNIPNMFVDSS 309
>sp|O94905|SPFH2_HUMAN SPFH domain protein 2 precursor
 sp|Q5R7C5|SPFH2_PONPY SPFH domain protein 2 precursor
          Length = 339

 Score =  135 bits (339), Expect = 1e-31
 Identities = 68/117 (58%), Positives = 94/117 (80%)
 Frame = +2

Query: 2   IASERQRVVEKEAETERLRAIIEAEKLAQVSRIHWQMKITEKESELNISRIEDAARTSRI 181
           IA+++Q+VVEKEAETER +A+IEAEK+AQV+ I +  K+ EKE+E  IS IEDAA  +R 
Sbjct: 195 IAAQKQKVVEKEAETERKKALIEAEKVAQVAEITYGQKVMEKETEKKISEIEDAAFLARE 254

Query: 182 KSESDAEFYRAKQEAEANKVKLTQEYLEMIRLLSIANNTKIYFGNSIPQMFLDSSNS 352
           K+++DAE Y A + AEANK+KLT EYL++++  +IA+N+KIYFG  IP MF+DS+ S
Sbjct: 255 KAKADAECYTAMKIAEANKLKLTPEYLQLMKYKAIASNSKIYFGKDIPNMFMDSAGS 311
>sp|Q8BFZ9|SPFH2_MOUSE SPFH domain protein 2 precursor
          Length = 340

 Score =  134 bits (336), Expect = 2e-31
 Identities = 72/138 (52%), Positives = 101/138 (73%), Gaps = 1/138 (0%)
 Frame = +2

Query: 2   IASERQRVVEKEAETERLRAIIEAEKLAQVSRIHWQMKITEKESELNISRIEDAARTSRI 181
           IA+++Q+VVEKEAETER +A+IEAEK+AQV+ I +  K+ EKE+E  IS IEDAA  +R 
Sbjct: 195 IAAQKQKVVEKEAETERKKALIEAEKVAQVAEITYGQKVMEKETEKKISEIEDAAFLARE 254

Query: 182 KSESDAEFYRAKQEAEANKVKLTQEYLEMIRLLSIANNTKIYFGNSIPQMFLDSSNS-GQ 358
           K+++DAE Y A + AEANK+KLT EYL++++  +IA+N+KIYFG  IP MF+DS+   G+
Sbjct: 255 KAKADAECYTALKIAEANKLKLTPEYLQLMKYKAIASNSKIYFGKDIPNMFMDSAGGLGK 314

Query: 359 TNQDYSTSTPGIKLPNLP 412
             +  S    G  L + P
Sbjct: 315 QFEGLSDDKLGFGLEDEP 332
>sp|P08799|MYS2_DICDI Myosin II heavy chain, non muscle
          Length = 2116

 Score = 39.3 bits (90), Expect = 0.007
 Identities = 41/122 (33%), Positives = 53/122 (43%)
 Frame = +2

Query: 35   EAETERLRAIIEAEKLAQVSRIHWQMKITEKESELNISRIEDAARTSRIKSESDAEFYRA 214
            E E  RL+  +EAE+LA+ S +  Q K  E +       +ED       KS   AE   A
Sbjct: 1177 EGEVARLQGELEAEQLAK-SNVEKQKKKVELD-------LED-------KSAQLAEETAA 1221

Query: 215  KQEAEANKVKLTQEYLEMIRLLSIANNTKIYFGNSIPQMFLDSSNSGQTNQDYSTSTPGI 394
            KQ  +  K KL QE  E+   LS ANN  +              NS  TN+   TS   +
Sbjct: 1222 KQALDKLKKKLEQELSEVQTQLSEANNKNV--------------NSDSTNKHLETSFNNL 1267

Query: 395  KL 400
            KL
Sbjct: 1268 KL 1269
>sp|O13127|FLOT1_CARAU Flotillin-1 (Reggie-2) (REG-2)
          Length = 423

 Score = 35.4 bits (80), Expect = 0.11
 Identities = 21/82 (25%), Positives = 43/82 (52%), Gaps = 4/82 (4%)
 Frame = +2

Query: 23  VVEKEAETERLRAIIEAEKLAQVSRIHWQMKITEKESELNISRIEDAARTSRIKSE---- 190
           V  K+AE+E    +  A+   Q+     Q+ + E+  ++ +   E A +   ++++    
Sbjct: 227 VCTKKAESEMAYQLQVAKTKQQIEEEKMQVMVVERSQQIMLQEQEIARKEKELEAQVMKP 286

Query: 191 SDAEFYRAKQEAEANKVKLTQE 256
           +DAE YR ++ AEA +++L  E
Sbjct: 287 ADAERYRLEKLAEAERLQLIME 308
>sp|P23607|ZFA_MOUSE Zinc finger autosomal protein
          Length = 742

 Score = 34.3 bits (77), Expect = 0.23
 Identities = 22/77 (28%), Positives = 37/77 (48%), Gaps = 3/77 (3%)
 Frame = +2

Query: 23  VVEKEAETERLRAIIEAEKLAQVSR---IHWQMKITEKESELNISRIEDAARTSRIKSES 193
           +VE E E E    +++      V R   ++  +  +++E ELN++ I D      I  E 
Sbjct: 215 IVESEPENELGVELLDPNNSICVpreKMVYMAVNNSQQEEELNVTEIADEIYMEVIVGEE 274

Query: 194 DAEFYRAKQEAEANKVK 244
           DA     +Q+ E NK+K
Sbjct: 275 DATASEHEQQVEDNKMK 291
>sp|O61491|FLOT1_DROME Flotillin-1
          Length = 426

 Score = 33.9 bits (76), Expect = 0.31
 Identities = 26/93 (27%), Positives = 46/93 (49%), Gaps = 3/93 (3%)
 Frame = +2

Query: 2   IASERQRVVEKEAETER-LRAIIEAEK--LAQVSRIHWQMKITEKESELNISRIEDAART 172
           IA + Q ++ +E E E  +R   EAEK  + +++  + Q  + E E+E    RI   A  
Sbjct: 268 IAVQEQEIMRRERELEATIRRPAEAEKFRMEKLAEANKQRVVMEAEAEAESIRIRGEAEA 327

Query: 173 SRIKSESDAEFYRAKQEAEANKVKLTQEYLEMI 271
             I +++ AE  +   +AEA +       +EM+
Sbjct: 328 FAIAAKAKAEAEQMAMKAEAYREYREAAMVEML 360
>sp|P26038|MOES_HUMAN Moesin (Membrane-organizing extension spike protein)
          Length = 577

 Score = 33.5 bits (75), Expect = 0.40
 Identities = 36/94 (38%), Positives = 44/94 (46%), Gaps = 14/94 (14%)
 Frame = +2

Query: 35  EAETERLRAIIEAEKLAQVSRIHWQMKITEKESELNISR----------IEDAARTSRIK 184
           E E ER RA  EAEKLA+      Q     KE+ L  SR          +E A  T+RI 
Sbjct: 374 ELEQERKRAQSEAEKLAK----ERQEAEEAKEALLQASRDQKKTQEQLALEMAELTARI- 428

Query: 185 SESDAEFYRAKQEAEA----NKVKLTQEYLEMIR 274
             S  E  R K+E+EA     K ++ QE LE  R
Sbjct: 429 --SQLEMARQKKESEAVEWQQKAQMVQEDLEKTR 460
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57,599,430
Number of Sequences: 369166
Number of extensions: 1068549
Number of successful extensions: 3073
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2954
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3060
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4357244645
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)