Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_010_E20-2
(441 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P00558|PGK1_HUMAN Phosphoglycerate kinase 1 (Primer reco... 163 1e-40
sp|Q60HD8|PGK1_MACFA Phosphoglycerate kinase 1 163 1e-40
sp|Q7SIB7|PGK1_PIG Phosphoglycerate kinase 1 162 4e-40
sp|P00559|PGK1_HORSE Phosphoglycerate kinase 1 162 4e-40
sp|P50310|PGK1_CRIGR Phosphoglycerate kinase 1 160 9e-40
sp|P16617|PGK1_RAT Phosphoglycerate kinase 1 160 9e-40
sp|P09411|PGK1_MOUSE Phosphoglycerate kinase 1 160 1e-39
sp|Q8MIF7|PGK2_HORSE Phosphoglycerate kinase, testis specific 159 2e-39
sp|Q6RI85|PGK2_PIG Phosphoglycerate kinase, testis specific 159 2e-39
sp|P09041|PGK2_MOUSE Phosphoglycerate kinase, testis specific 157 1e-38
>sp|P00558|PGK1_HUMAN Phosphoglycerate kinase 1 (Primer recognition protein 2) (PRP 2)
Length = 417
Score = 163 bits (413), Expect = 1e-40
Identities = 80/122 (65%), Positives = 91/122 (74%)
Frame = +2
Query: 2 AKVGHATLATGVPAGSMGLDCGSETMKDFCAAITRAKTIVWNGPMGVFEFENFAHGTKAA 181
AK G AT+A+G+PAG MGLDCG E+ K + A+TRAK IVWNGP+GVFE+E FA GTKA
Sbjct: 296 AKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKAL 355
Query: 182 MDAVVAATKSXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALS 361
MD VV AT CCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS
Sbjct: 356 MDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALS 415
Query: 362 DL 367
++
Sbjct: 416 NI 417
>sp|Q60HD8|PGK1_MACFA Phosphoglycerate kinase 1
Length = 417
Score = 163 bits (413), Expect = 1e-40
Identities = 80/122 (65%), Positives = 91/122 (74%)
Frame = +2
Query: 2 AKVGHATLATGVPAGSMGLDCGSETMKDFCAAITRAKTIVWNGPMGVFEFENFAHGTKAA 181
AK G AT+A+G+PAG MGLDCG E+ K + A+TRAK IVWNGP+GVFE+E FA GTKA
Sbjct: 296 AKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFAQGTKAL 355
Query: 182 MDAVVAATKSXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALS 361
MD VV AT CCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS
Sbjct: 356 MDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALS 415
Query: 362 DL 367
++
Sbjct: 416 NI 417
>sp|Q7SIB7|PGK1_PIG Phosphoglycerate kinase 1
Length = 417
Score = 162 bits (409), Expect = 4e-40
Identities = 79/122 (64%), Positives = 91/122 (74%)
Frame = +2
Query: 2 AKVGHATLATGVPAGSMGLDCGSETMKDFCAAITRAKTIVWNGPMGVFEFENFAHGTKAA 181
AK+G AT+A+G+PAG MGLDCG E+ K + A+ RAK IVWNGP+GVFE+E FA GTKA
Sbjct: 296 AKIGQATVASGIPAGWMGLDCGPESSKKYSEAVARAKQIVWNGPVGVFEWEAFAQGTKAL 355
Query: 182 MDAVVAATKSXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALS 361
MD VV AT CCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS
Sbjct: 356 MDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALS 415
Query: 362 DL 367
++
Sbjct: 416 NV 417
>sp|P00559|PGK1_HORSE Phosphoglycerate kinase 1
Length = 417
Score = 162 bits (409), Expect = 4e-40
Identities = 79/122 (64%), Positives = 91/122 (74%)
Frame = +2
Query: 2 AKVGHATLATGVPAGSMGLDCGSETMKDFCAAITRAKTIVWNGPMGVFEFENFAHGTKAA 181
AK G AT+A+G+PAG MGLDCG+E+ K + A+ RAK IVWNGP+GVFE+E FA GTKA
Sbjct: 296 AKTGQATVASGIPAGWMGLDCGTESSKKYAEAVARAKQIVWNGPVGVFEWEAFARGTKAL 355
Query: 182 MDAVVAATKSXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALS 361
MD VV AT CCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS
Sbjct: 356 MDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALS 415
Query: 362 DL 367
++
Sbjct: 416 NV 417
>sp|P50310|PGK1_CRIGR Phosphoglycerate kinase 1
Length = 417
Score = 160 bits (406), Expect = 9e-40
Identities = 78/122 (63%), Positives = 91/122 (74%)
Frame = +2
Query: 2 AKVGHATLATGVPAGSMGLDCGSETMKDFCAAITRAKTIVWNGPMGVFEFENFAHGTKAA 181
AK G AT+A+G+PAG MGLDCG+E+ K + A+ RAK IVWNGP+GVFE+E FA GTK+
Sbjct: 296 AKTGQATVASGIPAGWMGLDCGTESSKKYAEAVARAKQIVWNGPVGVFEWEAFARGTKSL 355
Query: 182 MDAVVAATKSXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALS 361
MD VV AT CCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS
Sbjct: 356 MDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALS 415
Query: 362 DL 367
++
Sbjct: 416 NV 417
>sp|P16617|PGK1_RAT Phosphoglycerate kinase 1
Length = 417
Score = 160 bits (406), Expect = 9e-40
Identities = 78/122 (63%), Positives = 91/122 (74%)
Frame = +2
Query: 2 AKVGHATLATGVPAGSMGLDCGSETMKDFCAAITRAKTIVWNGPMGVFEFENFAHGTKAA 181
AK G AT+A+G+PAG MGLDCG+E+ K + A+ RAK IVWNGP+GVFE+E FA GTK+
Sbjct: 296 AKTGQATVASGIPAGWMGLDCGTESSKKYAEAVARAKQIVWNGPVGVFEWEAFARGTKSL 355
Query: 182 MDAVVAATKSXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALS 361
MD VV AT CCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS
Sbjct: 356 MDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALS 415
Query: 362 DL 367
++
Sbjct: 416 NV 417
>sp|P09411|PGK1_MOUSE Phosphoglycerate kinase 1
Length = 417
Score = 160 bits (404), Expect = 1e-39
Identities = 78/122 (63%), Positives = 91/122 (74%)
Frame = +2
Query: 2 AKVGHATLATGVPAGSMGLDCGSETMKDFCAAITRAKTIVWNGPMGVFEFENFAHGTKAA 181
AK G AT+A+G+PAG MGLDCG+E+ K + A+ RAK IVWNGP+GVFE+E FA GTK+
Sbjct: 296 AKTGQATVASGIPAGWMGLDCGTESSKKYAEAVGRAKQIVWNGPVGVFEWEAFARGTKSL 355
Query: 182 MDAVVAATKSXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALS 361
MD VV AT CCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS
Sbjct: 356 MDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALS 415
Query: 362 DL 367
++
Sbjct: 416 NV 417
>sp|Q8MIF7|PGK2_HORSE Phosphoglycerate kinase, testis specific
Length = 417
Score = 159 bits (403), Expect = 2e-39
Identities = 79/122 (64%), Positives = 90/122 (73%)
Frame = +2
Query: 2 AKVGHATLATGVPAGSMGLDCGSETMKDFCAAITRAKTIVWNGPMGVFEFENFAHGTKAA 181
AKVG AT+A+G+PAG MGLDCG ET K + + +AK IVWNGP+GVFE++ FA GTKA
Sbjct: 296 AKVGQATVASGIPAGWMGLDCGPETNKKYAQVMAQAKLIVWNGPVGVFEWDAFAKGTKAL 355
Query: 182 MDAVVAATKSXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALS 361
MD +V AT CCAKW TEDKVSHVSTGGGASLELLEGKILPGV ALS
Sbjct: 356 MDEIVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKILPGVDALS 415
Query: 362 DL 367
+L
Sbjct: 416 NL 417
>sp|Q6RI85|PGK2_PIG Phosphoglycerate kinase, testis specific
Length = 417
Score = 159 bits (403), Expect = 2e-39
Identities = 77/122 (63%), Positives = 90/122 (73%)
Frame = +2
Query: 2 AKVGHATLATGVPAGSMGLDCGSETMKDFCAAITRAKTIVWNGPMGVFEFENFAHGTKAA 181
AKVG AT+A+G+PAG + LDCG ET K + + RAK IVWNGP+GVFE++ FA+GTKA
Sbjct: 296 AKVGQATVASGIPAGWVALDCGPETNKKYAQVVARAKLIVWNGPLGVFEWDAFANGTKAL 355
Query: 182 MDAVVAATKSXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALS 361
MD +V AT CCAKW TEDKVSHVSTGGGASLELLEGK+LPGV ALS
Sbjct: 356 MDEIVKATSKGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVEALS 415
Query: 362 DL 367
+L
Sbjct: 416 NL 417
>sp|P09041|PGK2_MOUSE Phosphoglycerate kinase, testis specific
Length = 417
Score = 157 bits (396), Expect = 1e-38
Identities = 77/122 (63%), Positives = 91/122 (74%)
Frame = +2
Query: 2 AKVGHATLATGVPAGSMGLDCGSETMKDFCAAITRAKTIVWNGPMGVFEFENFAHGTKAA 181
AKVG AT+ +G+P+G MGLDCG E++K + +AK IVWNGP+GVFE++ FA GTKA
Sbjct: 296 AKVGQATIESGIPSGWMGLDCGPESIKINAQIVAQAKLIVWNGPIGVFEWDAFAKGTKAL 355
Query: 182 MDAVVAATKSXXXXXXXXXXXXXCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVAALS 361
MD VV AT + CCAKWGTEDKVSHVSTGGGASLELLEGKILPGV ALS
Sbjct: 356 MDEVVKATSNGCVTIIGGGDTATCCAKWGTEDKVSHVSTGGGASLELLEGKILPGVEALS 415
Query: 362 DL 367
++
Sbjct: 416 NM 417
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57,664,200
Number of Sequences: 369166
Number of extensions: 1210241
Number of successful extensions: 3810
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3524
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3626
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2294548080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)