Planarian EST Database


Dr_sW_010_C18-2

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_010_C18-2
         (494 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P60686|MNHD_STAAU  Na(+)/H(+) antiporter subunit D (Mnh c...    30   2.4  
sp|Q4H174|MATK_WATAN  Maturase K (Intron maturase)                 30   3.1  
sp|P57601|TSGA_BUCAI  TsgA protein homolog                         30   3.1  
sp|Q8CIF6|SIDT2_MOUSE  SID1 transmembrane family member 2 pr...    30   4.1  
sp|Q4H186|MATK_PILTE  Maturase K (Intron maturase)                 30   4.1  
sp|P53223|CAX4_YEAST  Dolichyldiphosphatase (Dolichyl pyroph...    30   4.1  
sp|P40144|ADCY5_RABIT  Adenylate cyclase type V (ATP pyropho...    29   5.3  
sp|Q9EQQ3|GPR63_MOUSE  Probable G-protein coupled receptor 6...    29   5.3  
sp|P30082|GLR_RAT  Glucagon receptor precursor (GL-R)              29   6.9  
sp|Q4H192|MATK_MICJU  Maturase K (Intron maturase)                 29   6.9  
>sp|P60686|MNHD_STAAU Na(+)/H(+) antiporter subunit D (Mnh complex subunit D)
 sp|P60687|MNHD_STAAW Na(+)/H(+) antiporter subunit D (Mnh complex subunit D)
 sp|P60685|MNHD_STAAN Na(+)/H(+) antiporter subunit D (Mnh complex subunit D)
 sp|P60684|MNHD_STAAM Na(+)/H(+) antiporter subunit D (Mnh complex subunit D)
          Length = 498

 Score = 30.4 bits (67), Expect = 2.4
 Identities = 19/47 (40%), Positives = 27/47 (57%)
 Frame = +3

Query: 219 LFSMLFIIR*SNAIVAVVIYYLYISFQLILAVYFYYYTRLFVNENII 359
           +FS L II  S  I A+VI Y Y +  +    Y+YY++ LF+   II
Sbjct: 78  IFSALLIIT-SIIITAIVILYSYQTIGIERERYYYYFSVLFMLIGII 123
>sp|Q4H174|MATK_WATAN Maturase K (Intron maturase)
          Length = 522

 Score = 30.0 bits (66), Expect = 3.1
 Identities = 32/125 (25%), Positives = 54/125 (43%), Gaps = 4/125 (3%)
 Frame = +1

Query: 82  EIVQFFLIGIPETSYLRADLTIRINLNKKFI--NKTVCAICGNTPYINYSRCYLLSDSPM 255
           +I+Q ++  +P   +LR  L    N N  FI  NK++               YL S    
Sbjct: 172 QILQCWIQDVPLLHFLRFFLHEYNNWNSFFITQNKSI---------------YLFSKETK 216

Query: 256 QSWLWLFIICILAFNLFWLFIFITTLACL*MKISFRKFIQRNYKYEQYKEKLT--ILIKC 429
           +  L+ F+     +   +LF+F+   +      SFR F++R Y Y + +   T  ++I C
Sbjct: 217 R--LFRFLYNSYVYECEFLFVFLRKHSSYLRFTSFRTFLERRYFYGKMEHLQTEHLIIVC 274

Query: 430 MK*FN 444
              FN
Sbjct: 275 CDXFN 279
>sp|P57601|TSGA_BUCAI TsgA protein homolog
          Length = 388

 Score = 30.0 bits (66), Expect = 3.1
 Identities = 14/42 (33%), Positives = 24/42 (57%), Gaps = 2/42 (4%)
 Frame = +1

Query: 265 LWLFIICILAFNLFWLFIFITTLACL*MK--ISFRKFIQRNY 384
           +W F   I  FNL+ +FIF+T+++ + M   I    F+ + Y
Sbjct: 257 MWFFSFIIKFFNLYRMFIFLTSMSTILMYCFIKSENFLNQQY 298
>sp|Q8CIF6|SIDT2_MOUSE SID1 transmembrane family member 2 precursor
          Length = 832

 Score = 29.6 bits (65), Expect = 4.1
 Identities = 13/53 (24%), Positives = 25/53 (47%)
 Frame = +1

Query: 25  KYLNNEIIKCYITPALENNEIVQFFLIGIPETSYLRADLTIRINLNKKFINKT 183
           KYL  ++ +    P  +N   +QFF + +   S +     +R+N    F+ +T
Sbjct: 106 KYLYQKVERTLCQPPTKNESEIQFFYVDVSTLSPVNTTYQLRVNRVDNFVLRT 158
>sp|Q4H186|MATK_PILTE Maturase K (Intron maturase)
          Length = 522

 Score = 29.6 bits (65), Expect = 4.1
 Identities = 31/125 (24%), Positives = 54/125 (43%), Gaps = 4/125 (3%)
 Frame = +1

Query: 82  EIVQFFLIGIPETSYLRADLTIRINLNKKFI--NKTVCAICGNTPYINYSRCYLLSDSPM 255
           +I+Q ++  +P   +LR  L    N N  FI  NK++               YL S    
Sbjct: 172 QILQCWIQDVPLLHFLRFFLHEYHNWNSFFITQNKSI---------------YLFSKETK 216

Query: 256 QSWLWLFIICILAFNLFWLFIFITTLACL*MKISFRKFIQRNYKYEQYKEKLT--ILIKC 429
           +  L+ F+     +   ++F+F+   +      SFR F++R Y Y + +   T  ++I C
Sbjct: 217 R--LFRFLYNSYVYECEFVFVFLRKYSSYLRFTSFRTFLERRYFYGKMEHLQTEHLIIVC 274

Query: 430 MK*FN 444
              FN
Sbjct: 275 CDYFN 279
>sp|P53223|CAX4_YEAST Dolichyldiphosphatase (Dolichyl pyrophosphate phosphatase)
          Length = 239

 Score = 29.6 bits (65), Expect = 4.1
 Identities = 20/62 (32%), Positives = 28/62 (45%), Gaps = 5/62 (8%)
 Frame = +1

Query: 172 INKTVCAICGNTPYINYSRCYLLSDSP-----MQSWLWLFIICILAFNLFWLFIFITTLA 336
           +N T  AI  N   I +   Y+L DS      + ++  L  I +LAF L W  I     A
Sbjct: 1   MNSTAAAINPNPNVIPFDDTYILYDSHDFLSFLSAYFSLMPILVLAFYLSWFIITRELEA 60

Query: 337 CL 342
           C+
Sbjct: 61  CI 62
>sp|P40144|ADCY5_RABIT Adenylate cyclase type V (ATP pyrophosphate-lyase 5) (Adenylyl
           cyclase 5)
          Length = 1264

 Score = 29.3 bits (64), Expect = 5.3
 Identities = 14/36 (38%), Positives = 21/36 (58%)
 Frame = -2

Query: 133 LLNTKFLVYRLRKIVQFHYFPKQVLCSILLFRCLNI 26
           LL+T  L   LR   Q  +  KQ++ ++L+F C NI
Sbjct: 359 LLSTLHLAIALRTNAQDRFLLKQLVSNVLIFSCTNI 394
>sp|Q9EQQ3|GPR63_MOUSE Probable G-protein coupled receptor 63 (PSP24-beta) (PSP24-2)
          Length = 425

 Score = 29.3 bits (64), Expect = 5.3
 Identities = 17/63 (26%), Positives = 28/63 (44%), Gaps = 1/63 (1%)
 Frame = +1

Query: 262 WLWLFIICILAFNLFWLFIFITTLACL*MKIS-FRKFIQRNYKYEQYKEKLTILIKCMK* 438
           W++    C L+   FWLF+       L + I  F   +QR  K   Y+ K+ I +     
Sbjct: 153 WIFGKFFCRLSAMFFWLFVIEGVAILLIISIDRFLIIVQRQDKLNPYRAKVLIAVSWATA 212

Query: 439 FNI 447
           F++
Sbjct: 213 FSV 215
>sp|P30082|GLR_RAT Glucagon receptor precursor (GL-R)
          Length = 485

 Score = 28.9 bits (63), Expect = 6.9
 Identities = 31/147 (21%), Positives = 61/147 (41%), Gaps = 11/147 (7%)
 Frame = +1

Query: 13  SKSLKYLNNEIIKCYITPALENNEIVQFFLIGIPETSYLRADLTIRINLNKKFINKTVCA 192
           S S+   +  +  C +   +    I+  +   + E  YL + L+I     K F +  +C 
Sbjct: 212 SVSVWLSDGAVAGCRVATVIMQYGIIANYCWLLVEGVYLYSLLSITTFSEKSFFSLYLCI 271

Query: 193 ICGNT-----PYINYS------RCYLLSDSPMQSWLWLFIICILAFNLFWLFIFITTLAC 339
             G+      P++         +C+  +D+ M  W W+  I +L   L   FIF+  +  
Sbjct: 272 GWGSPLLFVIPWVVVKCLFENVQCWTSNDN-MGFW-WILRIPVLLAILINFFIFVRIIHL 329

Query: 340 L*MKISFRKFIQRNYKYEQYKEKLTIL 420
           L  K+   +    +YK+   +  LT++
Sbjct: 330 LVAKLRAHQMHYADYKFRLARSTLTLI 356
>sp|Q4H192|MATK_MICJU Maturase K (Intron maturase)
          Length = 522

 Score = 28.9 bits (63), Expect = 6.9
 Identities = 29/123 (23%), Positives = 52/123 (42%), Gaps = 2/123 (1%)
 Frame = +1

Query: 82  EIVQFFLIGIPETSYLRADLTIRINLNKKFINKTVCAICGNTPYINYSRCYLLSDSPMQS 261
           +I+Q ++  +P   +LR  L    N N  FI +              +  YL S    + 
Sbjct: 172 QILQCWIQDVPLLHFLRFFLHEYHNWNSFFITQN-------------NSIYLFSKETKR- 217

Query: 262 WLWLFIICILAFNLFWLFIFITTLACL*MKISFRKFIQRNYKYEQYKEKLT--ILIKCMK 435
            L+ F+     +   ++F+F+   +      SFR F++R Y Y + +   T  ++I C  
Sbjct: 218 -LFRFLYNSYVYECEFVFVFLRKHSSYLRFTSFRTFLERRYFYVKMEHLQTEHLIIVCCD 276

Query: 436 *FN 444
            FN
Sbjct: 277 YFN 279
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 48,715,100
Number of Sequences: 369166
Number of extensions: 944458
Number of successful extensions: 2546
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2464
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2543
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 3069744620
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)