Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_009_N24
(485 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9Y7J6|KOIA_SCHPO Putative serine/threonine-protein kina... 78 9e-15
sp|Q9DB30|PHKG2_MOUSE Phosphorylase b kinase gamma catalyti... 73 4e-13
sp|P31325|PHKG2_RAT Phosphorylase b kinase gamma catalytic ... 72 9e-13
sp|P15735|PHKG2_HUMAN Phosphorylase b kinase gamma catalyti... 72 9e-13
sp|P42681|TXK_HUMAN Tyrosine-protein kinase TXK 70 3e-12
sp|P42687|SPK1_DUGTI Tyrosine-protein kinase SPK-1 69 4e-12
sp|P57059|SN1L1_HUMAN Serine/threonine-protein kinase SNF1-... 69 4e-12
sp|P16591|FER_HUMAN Proto-oncogene tyrosine-protein kinase ... 69 4e-12
sp|Q12236|KOK0_YEAST Probable serine/threonine-protein kina... 69 6e-12
sp|Q03147|CDK7_MOUSE Cell division protein kinase 7 (CDK-ac... 69 6e-12
>sp|Q9Y7J6|KOIA_SCHPO Putative serine/threonine-protein kinase C1778.10c
Length = 550
Score = 78.2 bits (191), Expect = 9e-15
Identities = 52/170 (30%), Positives = 90/170 (52%), Gaps = 12/170 (7%)
Frame = +3
Query: 3 DYITKGNFSELRRGTFRNTNTE----ILIRKYEVKSSKTWKDEEFMLKELITFQMTKSHR 170
D I G+FS++RR T + + E +L +KY VK +K +++ E +
Sbjct: 58 DIIGDGSFSKVRRATDKKSWKEYAIKVLDKKYIVKENKV----KYVNIERDSMMRLNGFP 113
Query: 171 RFIQLLGVYIDDAILYVVMENMDRGTLKTFLQENYKNLDKNKIYKWSAQILEGLKFLHEN 350
+L + DD LY V+E G L ++++ Y+ LD+N + ++A+IL ++++H
Sbjct: 114 GISRLFHTFQDDLKLYYVLELAPNGELLQYIKK-YRFLDENCVRFYAAEILSSIEYMHSC 172
Query: 351 KVIHRDLNPSSILVDGNEDLKLSSFNLSKYL--------DISKAPASIMG 476
+IHRDL P +IL DGN +K++ F +K L D + P+S +G
Sbjct: 173 GIIHRDLKPENILFDGNMHVKITDFGTAKILPPKYVNSPDYTTFPSSFVG 222
>sp|Q9DB30|PHKG2_MOUSE Phosphorylase b kinase gamma catalytic chain, testis/liver isoform
(PHK-gamma-T) (Phosphorylase kinase gamma subunit 2)
Length = 406
Score = 72.8 bits (177), Expect = 4e-13
Identities = 44/163 (26%), Positives = 78/163 (47%), Gaps = 5/163 (3%)
Frame = +3
Query: 3 DYITKGNFSELRRGTFRNTNTEILIRKYEVKSSKTWKDE-----EFMLKELITFQMTKSH 167
D I +G S +RR R T E ++ EV + + ++ + +E+ + H
Sbjct: 28 DIIGRGVSSVVRRCVHRATGDEFAVKIMEVSAERLSLEQLEEVRDATRREMHILRQVAGH 87
Query: 168 RRFIQLLGVYIDDAILYVVMENMDRGTLKTFLQENYKNLDKNKIYKWSAQILEGLKFLHE 347
I L+ Y + +++V + M +G L +L E L + + +LE + FLH
Sbjct: 88 PHIITLIDSYESSSFMFLVFDLMRKGELFDYLTEKVA-LSEKETRSIMRSLLEAVSFLHA 146
Query: 348 NKVIHRDLNPSSILVDGNEDLKLSSFNLSKYLDISKAPASIMG 476
N ++HRDL P +IL+D N ++LS F S +L+ + + G
Sbjct: 147 NNIVHRDLKPENILLDDNMQIRLSDFGFSCHLEAGEKLRELCG 189
>sp|P31325|PHKG2_RAT Phosphorylase b kinase gamma catalytic chain, testis/liver isoform
(PHK-gamma-T) (Phosphorylase kinase gamma subunit 2)
Length = 406
Score = 71.6 bits (174), Expect = 9e-13
Identities = 43/153 (28%), Positives = 75/153 (49%), Gaps = 5/153 (3%)
Frame = +3
Query: 3 DYITKGNFSELRRGTFRNTNTEILIRKYEVKSSKTWKDE-----EFMLKELITFQMTKSH 167
D I +G S +RR R T E ++ EV + + ++ + +E+ + H
Sbjct: 28 DIIGRGVSSVVRRCVHRATGDEFAVKIMEVSAERLSLEQLEEVRDATRREMHILRQVAGH 87
Query: 168 RRFIQLLGVYIDDAILYVVMENMDRGTLKTFLQENYKNLDKNKIYKWSAQILEGLKFLHE 347
I L+ Y + +++V + M +G L +L E L + + +LE + FLH
Sbjct: 88 PHIITLIDSYESSSFMFLVFDLMRKGELFDYLTEKVA-LSEKETRSIMRSLLEAVNFLHV 146
Query: 348 NKVIHRDLNPSSILVDGNEDLKLSSFNLSKYLD 446
N ++HRDL P +IL+D N ++LS F S +L+
Sbjct: 147 NNIVHRDLKPENILLDDNMQIRLSDFGFSCHLE 179
>sp|P15735|PHKG2_HUMAN Phosphorylase b kinase gamma catalytic chain, testis/liver isoform
(PHK-gamma-T) (Phosphorylase kinase gamma subunit 2)
(PSK-C3)
Length = 406
Score = 71.6 bits (174), Expect = 9e-13
Identities = 44/153 (28%), Positives = 74/153 (48%), Gaps = 5/153 (3%)
Frame = +3
Query: 3 DYITKGNFSELRRGTFRNTNTEILIRKYEVKSSKTWKDE-----EFMLKELITFQMTKSH 167
D I +G S +RR R T E ++ EV + + ++ E +E + H
Sbjct: 28 DVIGRGVSSVVRRCVHRATGHEFAVKIMEVTAERLSPEQLEEVREATRRETHILRQVAGH 87
Query: 168 RRFIQLLGVYIDDAILYVVMENMDRGTLKTFLQENYKNLDKNKIYKWSAQILEGLKFLHE 347
I L+ Y + +++V + M +G L +L E L + + +LE + FLH
Sbjct: 88 PHIITLIDSYESSSFMFLVFDLMRKGELFDYLTEKVA-LSEKETRSIMRSLLEAVSFLHA 146
Query: 348 NKVIHRDLNPSSILVDGNEDLKLSSFNLSKYLD 446
N ++HRDL P +IL+D N ++LS F S +L+
Sbjct: 147 NNIVHRDLKPENILLDDNMQIRLSDFGFSCHLE 179
>sp|P42681|TXK_HUMAN Tyrosine-protein kinase TXK
Length = 527
Score = 70.1 bits (170), Expect = 3e-12
Identities = 46/145 (31%), Positives = 73/145 (50%)
Frame = +3
Query: 9 ITKGNFSELRRGTFRNTNTEILIRKYEVKSSKTWKDEEFMLKELITFQMTKSHRRFIQLL 188
I G F + G +R+ + ++ I+ S +E+F+ E M SH + +QL
Sbjct: 277 IGSGQFGVVHLGEWRS-HIQVAIKAINEGSMS---EEDFI--EEAKVMMKLSHSKLVQLY 330
Query: 189 GVYIDDAILYVVMENMDRGTLKTFLQENYKNLDKNKIYKWSAQILEGLKFLHENKVIHRD 368
GV I LY+V E M+ G L +L+EN L K + I EG+++L N IHRD
Sbjct: 331 GVCIQRKPLYIVTEFMENGCLLNYLRENKGKLRKEMLLSVCQDICEGMEYLERNGYIHRD 390
Query: 369 LNPSSILVDGNEDLKLSSFNLSKYL 443
L + LV +K+S F +++Y+
Sbjct: 391 LAARNCLVSSTCIVKISDFGMTRYV 415
>sp|P42687|SPK1_DUGTI Tyrosine-protein kinase SPK-1
Length = 497
Score = 69.3 bits (168), Expect = 4e-12
Identities = 48/148 (32%), Positives = 84/148 (56%), Gaps = 8/148 (5%)
Frame = +3
Query: 27 SELRRGTFRNTNTEILIRKYEV-----KSSKTWKDEEFMLKELITFQMTKSHRRFIQLLG 191
+E+ RG F + + R YEV +SSK + E+F+L+ I ++ HR+ ++LLG
Sbjct: 224 NEIGRGFFGSVHRAKWGRSYEVAAKMLQSSKAER-EKFVLEAKIMHKLR--HRKIVELLG 280
Query: 192 VYID--DAILYVVMENMDRGTLKTFLQE-NYKNLDKNKIYKWSAQILEGLKFLHENKVIH 362
V + D + +++E M G+LK +L+ + K + N++ A+I EG+ +L KV+H
Sbjct: 281 VCTEPQDMPMLIIVEYMKNGSLKEYLKTPDGKKTNLNQMVHMMAEISEGMAYLESEKVVH 340
Query: 363 RDLNPSSILVDGNEDLKLSSFNLSKYLD 446
RDL +ILV + K++ F L++ D
Sbjct: 341 RDLRADNILVANDLTRKVADFGLTELTD 368
>sp|P57059|SN1L1_HUMAN Serine/threonine-protein kinase SNF1-like kinase 1
(Serine/threonine-protein kinase SNF1LK)
Length = 783
Score = 69.3 bits (168), Expect = 4e-12
Identities = 44/144 (30%), Positives = 75/144 (52%), Gaps = 2/144 (1%)
Frame = +3
Query: 15 KGNFSELRRGTFRNTNTEILIRKYEVKSSKTWKDEEFMLKELITFQMTK--SHRRFIQLL 188
KGNF+ ++ R T T++ I+ + KT D + K Q+ K +H I+L
Sbjct: 35 KGNFAVVKLARHRVTKTQVAIKIID----KTRLDSSNLEKIYREVQLMKLLNHPHIIKLY 90
Query: 189 GVYIDDAILYVVMENMDRGTLKTFLQENYKNLDKNKIYKWSAQILEGLKFLHENKVIHRD 368
V +LY+V E G + +L N +L +N+ K QIL +++ H++ ++HRD
Sbjct: 91 QVMETKDMLYIVTEFAKNGEMFDYLTSN-GHLSENEARKKFWQILSAVEYCHDHHIVHRD 149
Query: 369 LNPSSILVDGNEDLKLSSFNLSKY 440
L ++L+DGN D+KL+ F +
Sbjct: 150 LKTENLLLDGNMDIKLADFGFGNF 173
>sp|P16591|FER_HUMAN Proto-oncogene tyrosine-protein kinase FER (p94-FER) (c-FER)
Length = 822
Score = 69.3 bits (168), Expect = 4e-12
Identities = 44/150 (29%), Positives = 73/150 (48%), Gaps = 2/150 (1%)
Frame = +3
Query: 3 DYITKGNFSELRRGTFRNTNTEILIRKYEVKSSKTWKDEEFMLKELITFQMTKS--HRRF 176
+ + KGNF E+ +GT ++ + VK+ K +E +K L ++ K H
Sbjct: 567 ELLGKGNFGEVYKGTLKDKTS------VAVKTCKEDLPQELKIKFLQEAKILKQYDHPNI 620
Query: 177 IQLLGVYIDDAILYVVMENMDRGTLKTFLQENYKNLDKNKIYKWSAQILEGLKFLHENKV 356
++L+GV +Y++ME + G TFL+ L ++ K+S G+ +L
Sbjct: 621 VKLIGVCTQRQPVYIIMELVSGGDFLTFLRRKKDELKLKQLVKFSLDAAAGMLYLESKNC 680
Query: 357 IHRDLNPSSILVDGNEDLKLSSFNLSKYLD 446
IHRDL + LV N LK+S F +S+ D
Sbjct: 681 IHRDLAARNCLVGENNVLKISDFGMSRQED 710
>sp|Q12236|KOK0_YEAST Probable serine/threonine-protein kinase YOL100W
Length = 1081
Score = 68.9 bits (167), Expect = 6e-12
Identities = 39/129 (30%), Positives = 69/129 (53%)
Frame = +3
Query: 60 NTEILIRKYEVKSSKTWKDEEFMLKELITFQMTKSHRRFIQLLGVYIDDAILYVVMENMD 239
N E LIR+ +VK ++ E Q + ++L + D++ LY ++E
Sbjct: 211 NKEYLIRQKKVK---------YVSIEKTALQKLNNSPSVVRLFSTFQDESSLYFLLEYAP 261
Query: 240 RGTLKTFLQENYKNLDKNKIYKWSAQILEGLKFLHENKVIHRDLNPSSILVDGNEDLKLS 419
G + L + Y +LD+ ++AQI++ + +LH N +IHRD+ P +IL+DG +KL+
Sbjct: 262 NGDFLS-LMKKYGSLDETCARYYAAQIIDAIDYLHSNGIIHRDIKPENILLDGEMKIKLT 320
Query: 420 SFNLSKYLD 446
F +K L+
Sbjct: 321 DFGTAKLLN 329
>sp|Q03147|CDK7_MOUSE Cell division protein kinase 7 (CDK-activating kinase) (CAK) (TFIIH
basal transcription factor complex kinase subunit) (39
kDa protein kinase) (P39 Mo15) (Protein-tyrosine kinase
MPK-7) (CR4 protein kinase) (CRK4)
Length = 346
Score = 68.9 bits (167), Expect = 6e-12
Identities = 43/147 (29%), Positives = 81/147 (55%), Gaps = 2/147 (1%)
Frame = +3
Query: 3 DYITKGNFSELRRGTFRNTNTEILIRKYEVKSSKTWKD--EEFMLKELITFQMTKSHRRF 176
D++ +G F+ + + +NTN + I+K ++ KD L+E+ Q SH
Sbjct: 16 DFLGEGQFATVYKARDKNTNQIVAIKKIKLGHRSEAKDGINRTALREIKLLQEL-SHPNI 74
Query: 177 IQLLGVYIDDAILYVVMENMDRGTLKTFLQENYKNLDKNKIYKWSAQILEGLKFLHENKV 356
I LL + + + +V + M+ L+ +++N L + I + L+GL++LH++ +
Sbjct: 75 IGLLDAFGHKSNISLVFDFMETD-LEVIIKDNSLVLTPSHIKAYMLMTLQGLEYLHQHWI 133
Query: 357 IHRDLNPSSILVDGNEDLKLSSFNLSK 437
+HRDL P+++L+D N LKL+ F L+K
Sbjct: 134 LHRDLKPNNLLLDENGVLKLADFGLAK 160
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 56,678,198
Number of Sequences: 369166
Number of extensions: 1098127
Number of successful extensions: 4642
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4306
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4451
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 2921208590
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)