Planarian EST Database


Dr_sW_009_N13

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_009_N13
         (833 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9Z2G9|TIP30_MOUSE  Oxidoreductase HTATIP2                     163   6e-40
sp|Q9BUP3|TIP30_HUMAN  Oxidoreductase HTATIP2 (HIV-1 TAT-int...   160   4e-39
sp|P45469|YRAR_ECOLI  Hypothetical protein yraR                    84   5e-16
sp|P40008|YEJ4_YEAST  Hypothetical 25.1 kDa protein in PMI40...    69   2e-11
sp|Q04304|YMY0_YEAST  Hypothetical 24.9 kDa protein in RCA1-...    37   0.088
sp|O61898|SRE12_CAEEL  Serpentine receptor class epsilon-12 ...    32   2.8  
sp|P32582|CBS_YEAST  Cystathionine beta-synthase (Serine sul...    30   6.3  
sp|Q9BMX3|ERF1_OXYTR  Eukaryotic peptide chain release facto...    30   8.2  
>sp|Q9Z2G9|TIP30_MOUSE Oxidoreductase HTATIP2
          Length = 242

 Score =  163 bits (412), Expect = 6e-40
 Identities = 96/227 (42%), Positives = 139/227 (61%), Gaps = 1/227 (0%)
 Frame = +2

Query: 44  VFIVGHTGETGKSLLNLLVNDSRFKEIVLFGRRIIELKFGENDKKCV-QKVVDFDKLDDC 220
           VFI+G +GETGK LL  ++  + F ++ L GRR  +L F E   K V Q+VVDF+KLD  
Sbjct: 21  VFILGASGETGKVLLKEILGQNLFSKVTLIGRR--KLTFEEEAYKNVNQEVVDFEKLDVY 78

Query: 221 ADLFADNFDGGLCCLGTTRAKSGKDGFIKVDHDYILKVAELAKLGDCKQFHLVSSAGANS 400
           A  F  + D G CCLGTTR+K+G +GF++VD DY+LK AELAK G CK F+L+SS GA+ 
Sbjct: 79  ASAFQGH-DVGFCCLGTTRSKAGAEGFVRVDRDYVLKSAELAKAGGCKHFNLLSSRGADK 137

Query: 401 KSYFLYQQVKGQVEEEISNLKYSALSIYRPKILMGHREEFRFGEIFFKSLVKPIACINPT 580
            S FLY QVKG+VE ++  LK+  LS++RP +L+  R+E R GE   +     +     +
Sbjct: 138 SSSFLYLQVKGEVEAKVEELKFDRLSVFRPGVLLCDRQESRPGEWLARKFFGSLPDSWAS 197

Query: 581 LVTTPFEMLSKAMVSSFIKNYIELNSSGDLCTSKIIDNNEIFKLASE 721
               P   + +AM++S +       SSG +   ++++N  I  L  +
Sbjct: 198 GYAVPVVTVVRAMLNSLVS-----PSSGQM---ELLENKAILHLGKD 236
>sp|Q9BUP3|TIP30_HUMAN Oxidoreductase HTATIP2 (HIV-1 TAT-interactive protein 2) (30 kDa
           HIV-1 TAT-interacting protein)
          Length = 242

 Score =  160 bits (405), Expect = 4e-39
 Identities = 85/164 (51%), Positives = 113/164 (68%), Gaps = 1/164 (0%)
 Frame = +2

Query: 44  VFIVGHTGETGKSLLNLLVNDSRFKEIVLFGRRIIELKFGENDKKCV-QKVVDFDKLDDC 220
           VFI+G +GETG+ LL  ++    F ++ L GRR  +L F E   K V Q+VVDF+KLDD 
Sbjct: 21  VFILGASGETGRVLLKEILEQGLFSKVTLIGRR--KLTFDEEAYKNVNQEVVDFEKLDDY 78

Query: 221 ADLFADNFDGGLCCLGTTRAKSGKDGFIKVDHDYILKVAELAKLGDCKQFHLVSSAGANS 400
           A  F  + D G CCLGTTR K+G +GF++VD DY+LK AELAK G CK F+L+SS GA+ 
Sbjct: 79  ASAFQGH-DVGFCCLGTTRGKAGAEGFVRVDRDYVLKSAELAKAGGCKHFNLLSSKGADK 137

Query: 401 KSYFLYQQVKGQVEEEISNLKYSALSIYRPKILMGHREEFRFGE 532
            S FLY QVKG+VE ++  LK+   S++RP +L+  R+E R GE
Sbjct: 138 SSNFLYLQVKGEVEAKVEELKFDRYSVFRPGVLLCDRQESRPGE 181
>sp|P45469|YRAR_ECOLI Hypothetical protein yraR
          Length = 211

 Score = 84.0 bits (206), Expect = 5e-16
 Identities = 57/173 (32%), Positives = 85/173 (49%), Gaps = 1/173 (0%)
 Frame = +2

Query: 41  KVFIVGHTGETGKSLLNLLVNDSRFKEIVLFGRRIIELKFGENDKKCVQKVVDFDKLDDC 220
           +V I G TG  G  LL +L+N+ +   I    RR +       D   V    D  +L D 
Sbjct: 3   QVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPL------GDMPGVFNPHD-PQLSDA 55

Query: 221 ADLFADNFDGGLCCLGTTRAKSG-KDGFIKVDHDYILKVAELAKLGDCKQFHLVSSAGAN 397
                D  D   CCLGTTR ++G K+ FI  D+  ++  A   +    +   +VS+ GAN
Sbjct: 56  LAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLGAQHMLVVSAMGAN 115

Query: 398 SKSYFLYQQVKGQVEEEISNLKYSALSIYRPKILMGHREEFRFGEIFFKSLVK 556
           + S F Y +VKG++EE +    +  L+I RP +L+G R + R  E  F  L +
Sbjct: 116 AHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNETLFAPLFR 168
>sp|P40008|YEJ4_YEAST Hypothetical 25.1 kDa protein in PMI40-YND1 intergenic region
          Length = 231

 Score = 68.9 bits (167), Expect = 2e-11
 Identities = 53/157 (33%), Positives = 80/157 (50%), Gaps = 3/157 (1%)
 Frame = +2

Query: 50  IVGHTGETGKSLLNLLVNDSRFKEIVLFGRRIIELKFGENDKKCVQKVVDFDKLDDCADL 229
           ++G TG  G   L       +F ++    RR  EL F   DK  V  +V+ D     + L
Sbjct: 5   VLGATGLCGGGFLRHAQEAPQFSKVYAILRR--ELPFPATDK--VVAIVERDN-SKWSQL 59

Query: 230 FADNFDGGLC--CLGTTRAKSGK-DGFIKVDHDYILKVAELAKLGDCKQFHLVSSAGANS 400
             +  +  +    L TTRA +G  D   K+DHD  L++A+ AK   C+   LVSSAGA+ 
Sbjct: 60  ITNEMNPQVLFTALATTRAAAGGLDKQYKIDHDLNLQLAQAAKEKGCETIVLVSSAGAHP 119

Query: 401 KSYFLYQQVKGQVEEEISNLKYSALSIYRPKILMGHR 511
            S F Y ++KG++E ++  L +  + I RP  L+G R
Sbjct: 120 DSRFGYMKMKGEIERDVIALDFKHIIILRPGPLLGER 156
>sp|Q04304|YMY0_YEAST Hypothetical 24.9 kDa protein in RCA1-NPL6 intergenic region
          Length = 227

 Score = 36.6 bits (83), Expect = 0.088
 Identities = 36/156 (23%), Positives = 66/156 (42%), Gaps = 11/156 (7%)
 Frame = +2

Query: 41  KVFIVGHTGETGKSLLNLLVNDSRFKEIVLFGRRIIELKFGENDKKCVQKVVDFD--KLD 214
           KV +VG +G+ G+ L+N L  +  F   +   R   ++ + +N+      + D +   + 
Sbjct: 5   KVAVVGASGKVGRLLINQLKANDSFSTPLAIVRTQDQVNYFKNEVGVDASLTDIENASVS 64

Query: 215 DCADLFADNFDGGLCCLGTTRAKSGKDGFIKVDHDYILKVAELAKLGDCKQFHLVSSAGA 394
           +  D     +D  +   G      G +    VD D  +KV E  +    K+F +VS+  A
Sbjct: 65  EITDAI-KAYDAVVFSAGA--GGKGMERIFTVDLDGCIKVVEACEKAGIKRFVVVSALKA 121

Query: 395 NSKSYFL-------YQQVKGQVEEEI--SNLKYSAL 475
             + ++        Y   K   + E+  SNL Y+ L
Sbjct: 122 EDRDFWYNIKGLREYYIAKRSADREVRNSNLDYTIL 157
>sp|O61898|SRE12_CAEEL Serpentine receptor class epsilon-12 (Protein sre-12)
          Length = 341

 Score = 31.6 bits (70), Expect = 2.8
 Identities = 17/65 (26%), Positives = 34/65 (52%), Gaps = 2/65 (3%)
 Frame = +2

Query: 470 ALSIYRPKILMGHREEFRFGE--IFFKSLVKPIACINPTLVTTPFEMLSKAMVSSFIKNY 643
           A+ + R   L+G  ++F  G   +F  ++   + CI   + + PF +L +   + ++K+Y
Sbjct: 74  AIIVMRLHSLLGFTDDFDIGSNVVFNGAMTFSVYCIVAAMCSLPFSILERCFATRYLKDY 133

Query: 644 IELNS 658
            E NS
Sbjct: 134 -EANS 137
>sp|P32582|CBS_YEAST Cystathionine beta-synthase (Serine sulfhydrase) (Beta-thionase)
          Length = 507

 Score = 30.4 bits (67), Expect = 6.3
 Identities = 28/103 (27%), Positives = 41/103 (39%), Gaps = 2/103 (1%)
 Frame = +2

Query: 68  ETGKSLLNLLVNDSRFKEIVLFGR--RIIELKFGENDKKCVQKVVDFDKLDDCADLFADN 241
           + G   L +L  D +   +V      R + +    ND     K +DF KL++  D+ + N
Sbjct: 395 DNGFDQLPVLTEDGKLSGLVTLSELLRKLSINNSNNDNTIKGKYLDFKKLNNFNDVSSYN 454

Query: 242 FDGGLCCLGTTRAKSGKDGFIKVDHDYILKVAELAKLGDCKQF 370
                        KSGK  FIK D        E +KL D  +F
Sbjct: 455 -----------ENKSGKKKFIKFD--------ENSKLSDLNRF 478
>sp|Q9BMX3|ERF1_OXYTR Eukaryotic peptide chain release factor subunit 1 (eRF1)
           (Eukaryotic release factor 1)
          Length = 445

 Score = 30.0 bits (66), Expect = 8.2
 Identities = 24/88 (27%), Positives = 40/88 (45%)
 Frame = +2

Query: 83  LLNLLVNDSRFKEIVLFGRRIIELKFGENDKKCVQKVVDFDKLDDCADLFADNFDGGLCC 262
           LL+LL +D +F  I++ G   +      N ++ +QK+          +L   +  GG   
Sbjct: 141 LLSLLADDDKFGFIIVDGNGALYATLQGNSREILQKIT--------VELPKKHRKGGQSS 192

Query: 263 LGTTRAKSGKDGFIKVDHDYILKVAELA 346
           +   R +  K       H+Y+ KVAELA
Sbjct: 193 VRFARLREEKR------HNYLRKVAELA 214
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,015,785
Number of Sequences: 369166
Number of extensions: 1568767
Number of successful extensions: 3702
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 3582
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3695
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8100769320
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)