Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_009_M23
(385 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q61581|IBP7_MOUSE Insulin-like growth factor binding pro... 65 5e-11
sp|Q16270|IBP7_HUMAN Insulin-like growth factor binding pro... 64 8e-11
sp|Q8BJ66|KAZD1_MOUSE Kazal-type serine protease inhibitor ... 63 2e-10
sp|Q96I82|KAZD1_HUMAN Kazal-type serine protease inhibitor ... 62 5e-10
sp|Q13449|LSAMP_HUMAN Limbic system-associated membrane pro... 40 0.001
sp|Q98919|LSAMP_CHICK Limbic system-associated membrane pro... 40 0.002
sp|Q98892|OBCAM_CHICK Opioid-binding protein/cell adhesion ... 39 0.003
sp|P01005|IOVO_CHICK Ovomucoid precursor (Allergen Gal d 1)... 39 0.004
sp|P10184|IOV7_CHICK Ovoinhibitor precursor 39 0.005
sp|P25304|AGRN_RAT Agrin precursor 39 0.005
>sp|Q61581|IBP7_MOUSE Insulin-like growth factor binding protein 7 precursor (IGFBP-7)
(IBP-7) (IGF-binding protein 7) (MAC25 protein)
Length = 281
Score = 65.1 bits (157), Expect = 5e-11
Identities = 38/115 (33%), Positives = 57/115 (49%), Gaps = 14/115 (12%)
Frame = +1
Query: 43 GFCGNNMECVKRNDIDSLKYESQS-------VCVCQIKGLICGSDGITY-TPCQFAAAEM 198
G C MECVK K + + VCVC+ + +CGS+GITY + CQ AA +
Sbjct: 78 GHCAPGMECVKSRKRRKGKAGAAAGGPATLAVCVCKSRYPVCGSNGITYPSGCQLRAASL 137
Query: 199 KLK------ISQKHDGPCQTRPKVISASNDVNVAQGSKHTIMCEVQGFPLPTVQW 345
+ + I+Q G C+ P +++ D+ G+K + CEV G P P + W
Sbjct: 138 RAESRGEKAITQVSKGTCEQGPSIVTPPKDIWNVTGAKVFLSCEVIGIPTPVLIW 192
>sp|Q16270|IBP7_HUMAN Insulin-like growth factor binding protein 7 precursor (IGFBP-7)
(IBP-7) (IGF-binding protein 7) (MAC25 protein)
(Prostacyclin-stimulating factor) (PGI2-stimulating
factor) (IGFBP-rP1)
Length = 282
Score = 64.3 bits (155), Expect = 8e-11
Identities = 37/115 (32%), Positives = 56/115 (48%), Gaps = 14/115 (12%)
Frame = +1
Query: 43 GFCGNNMECVKRNDIDSLKYESQS-------VCVCQIKGLICGSDGITY-TPCQFAAAEM 198
G+C MECVK K + + VCVC+ + +CGSDG TY + CQ AA
Sbjct: 79 GYCAPGMECVKSRKRRKGKAGAAAGGPGVSGVCVCKSRYPVCGSDGTTYPSGCQLRAASQ 138
Query: 199 KLK------ISQKHDGPCQTRPKVISASNDVNVAQGSKHTIMCEVQGFPLPTVQW 345
+ + I+Q G C+ P +++ D+ G++ + CEV G P P + W
Sbjct: 139 RAESRGEKAITQVSKGTCEQGPSIVTPPKDIWNVTGAQVYLSCEVIGIPTPVLIW 193
>sp|Q8BJ66|KAZD1_MOUSE Kazal-type serine protease inhibitor domain 1 precursor
(IGFBP-related protein 10) (IGFBP-rP10) (Bone and
odontoblast-expressed protein 1)
Length = 313
Score = 62.8 bits (151), Expect = 2e-10
Identities = 31/108 (28%), Positives = 57/108 (52%), Gaps = 5/108 (4%)
Frame = +1
Query: 37 WHGFCGNNMECVKRNDIDSLKYE-SQSVCVCQIKGLICGSDGITYTPC----QFAAAEMK 201
++G CG +EC D + E + +CVC+ + +CGSDG TY + A A +
Sbjct: 105 FYGRCGEQLECRLDAGGDLSRGEVPEPLCVCRSQRPLCGSDGRTYAQICRLQEAARARLD 164
Query: 202 LKISQKHDGPCQTRPKVISASNDVNVAQGSKHTIMCEVQGFPLPTVQW 345
++ H GPC++ P+++S +++ G CEV +P+ +++W
Sbjct: 165 ANLTVVHPGPCESEPQILSQPHNIWNVTGQDVIFGCEVFAYPMASIEW 212
>sp|Q96I82|KAZD1_HUMAN Kazal-type serine protease inhibitor domain 1 precursor
Length = 304
Score = 61.6 bits (148), Expect = 5e-10
Identities = 31/108 (28%), Positives = 54/108 (50%), Gaps = 5/108 (4%)
Frame = +1
Query: 37 WHGFCGNNMECVKRNDIDSLKYE-SQSVCVCQIKGLICGSDGITYTPC----QFAAAEMK 201
++G CG +EC D + E + +C C+ + +CGSDG TY+ + A A
Sbjct: 98 FYGHCGEQLECRLDTGGDLSRGEVPEPLCACRSQSPLCGSDGHTYSQICRLQEAARARPD 157
Query: 202 LKISQKHDGPCQTRPKVISASNDVNVAQGSKHTIMCEVQGFPLPTVQW 345
++ H GPC++ P+++S D G CEV +P+ +++W
Sbjct: 158 ANLTVAHPGPCESGPQIVSHPYDTWNVTGQDVIFGCEVFAYPMASIEW 205
>sp|Q13449|LSAMP_HUMAN Limbic system-associated membrane protein precursor (LSAMP)
Length = 338
Score = 40.4 bits (93), Expect = 0.001
Identities = 18/46 (39%), Positives = 25/46 (54%)
Frame = +1
Query: 235 QTRPKVISASNDVNVAQGSKHTIMCEVQGFPLPTVQWFFTAPTTTE 372
Q PK+ + S+DV V +GS T++C G P P + W PT E
Sbjct: 129 QVPPKISNISSDVTVNEGSNVTLVCMANGRPEPVITWRHLTPTGRE 174
>sp|Q98919|LSAMP_CHICK Limbic system-associated membrane protein precursor (E19S) (CHLAMP,
G19-isoform)
Length = 338
Score = 40.0 bits (92), Expect = 0.002
Identities = 17/46 (36%), Positives = 25/46 (54%)
Frame = +1
Query: 235 QTRPKVISASNDVNVAQGSKHTIMCEVQGFPLPTVQWFFTAPTTTE 372
Q PK+ + S+D+ V +GS T++C G P P + W PT E
Sbjct: 129 QVPPKISNISSDITVNEGSNVTLVCMANGRPEPVITWRHLTPTGKE 174
>sp|Q98892|OBCAM_CHICK Opioid-binding protein/cell adhesion molecule homolog precursor
(Neurite inhibitor GP55-A) (OBCAM protein gamma isoform)
Length = 337
Score = 39.3 bits (90), Expect = 0.003
Identities = 16/37 (43%), Positives = 24/37 (64%)
Frame = +1
Query: 235 QTRPKVISASNDVNVAQGSKHTIMCEVQGFPLPTVQW 345
Q P++++ S+D+ V +GS T+MC G P PTV W
Sbjct: 126 QVPPQIVNISSDITVNEGSSVTLMCLAFGRPEPTVTW 162
Score = 28.5 bits (62), Expect = 5.0
Identities = 11/35 (31%), Positives = 16/35 (45%)
Frame = +1
Query: 244 PKVISASNDVNVAQGSKHTIMCEVQGFPLPTVQWF 348
P IS + + + G K + CE P+ QWF
Sbjct: 215 PPYISNAKNTGASVGQKGILQCEASAVPVAEFQWF 249
>sp|P01005|IOVO_CHICK Ovomucoid precursor (Allergen Gal d 1) (Gal d I)
Length = 210
Score = 38.9 bits (89), Expect = 0.004
Identities = 26/74 (35%), Positives = 38/74 (51%), Gaps = 5/74 (6%)
Frame = +1
Query: 103 ESQSVCVCQIKGL--ICGSDGITYT-PCQFAA--AEMKLKISQKHDGPCQTRPKVISASN 267
E + V VC K L ICG+DG+TYT C A E IS++HDG C+ + +S
Sbjct: 39 EGKDVLVCN-KDLRPICGTDGVTYTNDCLLCAYSIEFGTNISKEHDGECKETVPMNCSSY 97
Query: 268 DVNVAQGSKHTIMC 309
++ K ++C
Sbjct: 98 ANTTSEDGKVMVLC 111
Score = 31.2 bits (69), Expect = 0.76
Identities = 14/42 (33%), Positives = 22/42 (52%), Gaps = 3/42 (7%)
Frame = +1
Query: 142 ICGSDGITY-TPCQFAA--AEMKLKISQKHDGPCQTRPKVIS 258
+CG+DG+TY C A E + ++HDG C+ +S
Sbjct: 118 VCGTDGVTYDNECLLCAHKVEQGASVDKRHDGGCRKELAAVS 159
>sp|P10184|IOV7_CHICK Ovoinhibitor precursor
Length = 472
Score = 38.5 bits (88), Expect = 0.005
Identities = 29/94 (30%), Positives = 44/94 (46%), Gaps = 12/94 (12%)
Frame = +1
Query: 64 EC-VKRNDIDSLKYES------QSVCVC-QIKGLICGSDGITY-TPCQFAA--AEMKLKI 210
EC ++ +D KY S +++ C +I +CG+DG TY C A AE + +
Sbjct: 149 ECKLEIGSVDCSKYPSTVSKDGRTLVACPRILSPVCGTDGFTYDNECGICAHNAEQRTHV 208
Query: 211 SQKHDGPC-QTRPKVISASNDVNVAQGSKHTIMC 309
S+KHDG C Q P++ G K + C
Sbjct: 209 SKKHDGKCRQEIPEIDCDQYPTRKTTGGKLLVRC 242
Score = 35.0 bits (79), Expect = 0.053
Identities = 16/60 (26%), Positives = 33/60 (55%), Gaps = 4/60 (6%)
Frame = +1
Query: 142 ICGSDGITYT-PCQFAAAEMKL--KISQKHDGPCQTR-PKVISASNDVNVAQGSKHTIMC 309
+CG+DG+TY+ C A ++L +++KHDG C+ P++ + + + + + C
Sbjct: 314 VCGTDGVTYSNDCSLCAHNIELGTSVAKKHDGRCREEVPELDCSKYKTSTLKDGRQVVAC 373
Score = 32.0 bits (71), Expect = 0.45
Identities = 16/59 (27%), Positives = 28/59 (47%), Gaps = 3/59 (5%)
Frame = +1
Query: 142 ICGSDGITY-TPCQFAA--AEMKLKISQKHDGPCQTRPKVISASNDVNVAQGSKHTIMC 309
+CG+DG TY C A A+ ++ + HDG C+ R + + ++ Q + C
Sbjct: 249 VCGTDGFTYDNECGICAHNAQHGTEVKKSHDGRCKERSTPLDCTQYLSNTQNGEAITAC 307
Score = 30.4 bits (67), Expect = 1.3
Identities = 23/73 (31%), Positives = 29/73 (39%), Gaps = 12/73 (16%)
Frame = +1
Query: 142 ICGSDGITY-TPCQFAA--AEMKLKISQKHDGPCQTR---------PKVISASNDVNVAQ 285
+CGSD TY C A AE IS+ HDG C+ P +S VA
Sbjct: 117 VCGSDSFTYDNECGICAYNAEHHTNISKLHDGECKLEIGSVDCSKYPSTVSKDGRTLVAC 176
Query: 286 GSKHTIMCEVQGF 324
+ +C GF
Sbjct: 177 PRILSPVCGTDGF 189
Score = 28.9 bits (63), Expect = 3.8
Identities = 15/59 (25%), Positives = 28/59 (47%), Gaps = 3/59 (5%)
Frame = +1
Query: 142 ICGSDGITYT-PCQFAA--AEMKLKISQKHDGPCQTRPKVISASNDVNVAQGSKHTIMC 309
+CG+DG TY+ C E + +++DG C+ + +I S + V + + C
Sbjct: 52 VCGTDGSTYSNECGICLYNREHGANVEKEYDGECRPKHVMIDCSPYLQVVRDGNTMVAC 110
>sp|P25304|AGRN_RAT Agrin precursor
Length = 1959
Score = 38.5 bits (88), Expect = 0.005
Identities = 20/52 (38%), Positives = 30/52 (57%), Gaps = 8/52 (15%)
Frame = +1
Query: 106 SQSVCVCQI-----KGLICGSDGITY-TPCQF--AAAEMKLKISQKHDGPCQ 237
S CVC G +CGSDG+TY + C+ AA + +++I + H GPC+
Sbjct: 509 SAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEAHAGPCE 560
Score = 34.3 bits (77), Expect = 0.090
Identities = 21/65 (32%), Positives = 32/65 (49%), Gaps = 3/65 (4%)
Frame = +1
Query: 142 ICGSDGITY-TPCQF--AAAEMKLKISQKHDGPCQTRPKVISASNDVNVAQGSKHTIMCE 312
+CGSDG+TY CQ A +L IS + GPCQ V ++ + + + I+ +
Sbjct: 831 VCGSDGVTYGNECQLKAIACRQRLDISTQSLGPCQ--ESVTPGASPTSASMTTPRHILSK 888
Query: 313 VQGFP 327
FP
Sbjct: 889 TLPFP 893
Score = 33.1 bits (74), Expect = 0.20
Identities = 20/65 (30%), Positives = 28/65 (43%), Gaps = 3/65 (4%)
Frame = +1
Query: 49 CGNNMECVKRNDIDSLKYESQSVCVCQIKGLICGSDGITY-TPCQFA--AAEMKLKISQK 219
C CV D + + C G +CGSDG+ Y + CQ A + I +K
Sbjct: 148 CSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLSHACASQEHIFKK 207
Query: 220 HDGPC 234
+GPC
Sbjct: 208 FNGPC 212
Score = 29.3 bits (64), Expect = 2.9
Identities = 23/67 (34%), Positives = 28/67 (41%), Gaps = 8/67 (11%)
Frame = +1
Query: 142 ICGSDGITYT-PC--QFAAAEMKLKISQKHDGPCQTRPKVISASNDVNVAQGSKHTIM-- 306
+C DG TY C Q A + I KH GPC P S + V A G+ T+
Sbjct: 323 VCAQDGHTYNNDCWRQQAECRQQRAIPPKHQGPCDQTP---SPCHGVQCAFGAVCTVKNG 379
Query: 307 ---CEVQ 318
CE Q
Sbjct: 380 KAECECQ 386
Score = 28.9 bits (63), Expect = 3.8
Identities = 23/69 (33%), Positives = 31/69 (44%), Gaps = 4/69 (5%)
Frame = +1
Query: 40 HGF-CGNNMECVKRNDIDSLKYESQSVCVCQIKGLICGSDGITY-TPCQF--AAAEMKLK 207
HG C C +N + E Q VC I +CGSDG+TY + C+ A + +
Sbjct: 364 HGVQCAFGAVCTVKNG--KAECECQRVC-SGIYDPVCGSDGVTYGSVCELESMACTLGRE 420
Query: 208 ISQKHDGPC 234
I GPC
Sbjct: 421 IQVARRGPC 429
Score = 27.7 bits (60), Expect = 8.5
Identities = 15/37 (40%), Positives = 19/37 (51%), Gaps = 3/37 (8%)
Frame = +1
Query: 142 ICGSDGITY-TPCQFA--AAEMKLKISQKHDGPCQTR 243
ICG DG+TY C + A L + + G CQTR
Sbjct: 251 ICGDDGVTYENDCVMSRIGATRGLLLQKVRSGQCQTR 287
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 47,580,588
Number of Sequences: 369166
Number of extensions: 928698
Number of successful extensions: 2623
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2267
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2608
length of database: 68,354,980
effective HSP length: 94
effective length of database: 50,989,890
effective search space used: 1682666370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)