Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_009_M16
(395 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q95UN8|M3KSL_DROME Mitogen-activated protein kinase kina... 68 8e-12
sp|Q12469|SKM1_YEAST Serine/threonine-protein kinase SKM1 (... 67 1e-11
sp|P35465|PAK1_RAT Serine/threonine-protein kinase PAK 1 (p... 64 1e-10
sp|Q13153|PAK1_HUMAN Serine/threonine-protein kinase PAK 1 ... 63 2e-10
sp|Q64303|PAK2_RAT Serine/threonine-protein kinase PAK 2 (p... 62 3e-10
sp|O88643|PAK1_MOUSE Serine/threonine-protein kinase PAK 1 ... 62 3e-10
sp|Q8CIN4|PAK2_MOUSE Serine/threonine-protein kinase PAK 2 ... 62 3e-10
sp|Q13177|PAK2_HUMAN Serine/threonine-protein kinase PAK 2 ... 62 3e-10
sp|Q29502|PAK2_RABIT Serine/threonine-protein kinase PAK 2 ... 62 3e-10
sp|Q9ESL4|MLTK_MOUSE Mitogen-activated protein kinase kinas... 62 4e-10
>sp|Q95UN8|M3KSL_DROME Mitogen-activated protein kinase kinase kinase (Mixed lineage
kinase) (dMLK) (Slipper protein)
Length = 1161
Score = 67.8 bits (164), Expect = 8e-12
Identities = 37/98 (37%), Positives = 54/98 (55%), Gaps = 3/98 (3%)
Frame = +2
Query: 23 SKVGVYSYMAPEVLNSEPYDNAADVWSFAAVLVNLITGKNPCPDKDPIDAAFHVA---RK 193
S G Y++M PEV++ Y +DVWS+ +L LITG+ P DP+ A+ VA
Sbjct: 304 SAAGTYAWMPPEVISVSTYSKFSDVWSYGVLLWELITGETPYKGFDPLSVAYGVAVNTLT 363
Query: 194 LEMEYTLPEDTNQVLKDIITNCAQGDPKKRPKIKELIE 307
L + T PE ++K +C Q DP KRP KE+++
Sbjct: 364 LPIPKTCPETWGALMK----SCWQTDPHKRPGFKEILK 397
>sp|Q12469|SKM1_YEAST Serine/threonine-protein kinase SKM1 (Protein kinase 75490 D)
Length = 655
Score = 67.4 bits (163), Expect = 1e-11
Identities = 31/103 (30%), Positives = 56/103 (54%)
Frame = +2
Query: 5 KRSSSQSKVGVYSYMAPEVLNSEPYDNAADVWSFAAVLVNLITGKNPCPDKDPIDAAFHV 184
KRS + VG +MAPE++N + YD DVWS +L+ +I G+ P ++DP+ A + +
Sbjct: 535 KRSKRATMVGTPYWMAPEIVNQKGYDEKVDVWSLGIMLIEMIEGEPPYLNEDPLKALYLI 594
Query: 185 ARKLEMEYTLPEDTNQVLKDIITNCAQGDPKKRPKIKELIEID 313
A + PE ++ K + C Q + + R +++L+ +
Sbjct: 595 ANNGSPKLRHPESVSKQTKQFLDACLQVNVESRASVRKLLTFE 637
>sp|P35465|PAK1_RAT Serine/threonine-protein kinase PAK 1 (p21-activated kinase 1)
(PAK-1) (P68-PAK) (Alpha-PAK) (Protein kinase MUK2)
Length = 544
Score = 63.9 bits (154), Expect = 1e-10
Identities = 31/107 (28%), Positives = 56/107 (52%)
Frame = +2
Query: 5 KRSSSQSKVGVYSYMAPEVLNSEPYDNAADVWSFAAVLVNLITGKNPCPDKDPIDAAFHV 184
++S + VG +MAPEV+ + Y D+WS + + +I G+ P +++P+ A + +
Sbjct: 416 EQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLI 475
Query: 185 ARKLEMEYTLPEDTNQVLKDIITNCAQGDPKKRPKIKELIEIDLQKI 325
A E PE + + +D + C + D +KR KEL++ KI
Sbjct: 476 ATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFLKI 522
>sp|Q13153|PAK1_HUMAN Serine/threonine-protein kinase PAK 1 (p21-activated kinase 1)
(PAK-1) (P65-PAK) (Alpha-PAK)
Length = 545
Score = 63.2 bits (152), Expect = 2e-10
Identities = 31/107 (28%), Positives = 55/107 (51%)
Frame = +2
Query: 5 KRSSSQSKVGVYSYMAPEVLNSEPYDNAADVWSFAAVLVNLITGKNPCPDKDPIDAAFHV 184
++S + VG +MAPEV+ + Y D+WS + + +I G+ P +++P+ A + +
Sbjct: 417 EQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLI 476
Query: 185 ARKLEMEYTLPEDTNQVLKDIITNCAQGDPKKRPKIKELIEIDLQKI 325
A E PE + + +D + C D +KR KEL++ KI
Sbjct: 477 ATNGTPELQNPEKLSAIFRDFLNRCLDMDVEKRGSAKELLQHQFLKI 523
>sp|Q64303|PAK2_RAT Serine/threonine-protein kinase PAK 2 (p21-activated kinase 2)
(PAK-2) (Gamma-PAK) (p62-PAK)
Length = 524
Score = 62.4 bits (150), Expect = 3e-10
Identities = 29/107 (27%), Positives = 56/107 (52%)
Frame = +2
Query: 5 KRSSSQSKVGVYSYMAPEVLNSEPYDNAADVWSFAAVLVNLITGKNPCPDKDPIDAAFHV 184
++S + VG +MAPEV+ + Y D+WS + + ++ G+ P +++P+ A + +
Sbjct: 396 EQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLI 455
Query: 185 ARKLEMEYTLPEDTNQVLKDIITNCAQGDPKKRPKIKELIEIDLQKI 325
A E PE + + +D + C + D +KR KEL++ K+
Sbjct: 456 ATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGSAKELLQHPFLKL 502
>sp|O88643|PAK1_MOUSE Serine/threonine-protein kinase PAK 1 (p21-activated kinase 1)
(PAK-1) (P65-PAK) (Alpha-PAK) (CDC42/RAC effector kinase
PAK-A)
Length = 545
Score = 62.4 bits (150), Expect = 3e-10
Identities = 31/107 (28%), Positives = 56/107 (52%)
Frame = +2
Query: 5 KRSSSQSKVGVYSYMAPEVLNSEPYDNAADVWSFAAVLVNLITGKNPCPDKDPIDAAFHV 184
++S + VG +MAPEV+ + Y D+WS + + +I G+ P +++P+ A + +
Sbjct: 417 EQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLI 476
Query: 185 ARKLEMEYTLPEDTNQVLKDIITNCAQGDPKKRPKIKELIEIDLQKI 325
A E PE + + +D + C + D +KR KEL++ KI
Sbjct: 477 ATNGTPELQNPEKLSAIFRDFLQCCLEMDVEKRGSAKELLQHQFLKI 523
>sp|Q8CIN4|PAK2_MOUSE Serine/threonine-protein kinase PAK 2 (p21-activated kinase 2)
(PAK-2)
Length = 524
Score = 62.4 bits (150), Expect = 3e-10
Identities = 29/107 (27%), Positives = 56/107 (52%)
Frame = +2
Query: 5 KRSSSQSKVGVYSYMAPEVLNSEPYDNAADVWSFAAVLVNLITGKNPCPDKDPIDAAFHV 184
++S + VG +MAPEV+ + Y D+WS + + ++ G+ P +++P+ A + +
Sbjct: 396 EQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLI 455
Query: 185 ARKLEMEYTLPEDTNQVLKDIITNCAQGDPKKRPKIKELIEIDLQKI 325
A E PE + + +D + C + D +KR KEL++ K+
Sbjct: 456 ATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGSAKELLQHPFLKL 502
>sp|Q13177|PAK2_HUMAN Serine/threonine-protein kinase PAK 2 (p21-activated kinase 2)
(PAK-2) (PAK65) (Gamma-PAK) (S6/H4 kinase)
Length = 524
Score = 62.4 bits (150), Expect = 3e-10
Identities = 29/107 (27%), Positives = 56/107 (52%)
Frame = +2
Query: 5 KRSSSQSKVGVYSYMAPEVLNSEPYDNAADVWSFAAVLVNLITGKNPCPDKDPIDAAFHV 184
++S + VG +MAPEV+ + Y D+WS + + ++ G+ P +++P+ A + +
Sbjct: 396 EQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLI 455
Query: 185 ARKLEMEYTLPEDTNQVLKDIITNCAQGDPKKRPKIKELIEIDLQKI 325
A E PE + + +D + C + D +KR KEL++ K+
Sbjct: 456 ATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGSAKELLQHPFLKL 502
>sp|Q29502|PAK2_RABIT Serine/threonine-protein kinase PAK 2 (p21-activated kinase 2)
(PAK-2) (Gamma-PAK) (p21-activated protein kinase I)
(PAKI)
Length = 524
Score = 62.4 bits (150), Expect = 3e-10
Identities = 29/107 (27%), Positives = 56/107 (52%)
Frame = +2
Query: 5 KRSSSQSKVGVYSYMAPEVLNSEPYDNAADVWSFAAVLVNLITGKNPCPDKDPIDAAFHV 184
++S + VG +MAPEV+ + Y D+WS + + ++ G+ P +++P+ A + +
Sbjct: 396 EQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLI 455
Query: 185 ARKLEMEYTLPEDTNQVLKDIITNCAQGDPKKRPKIKELIEIDLQKI 325
A E PE + + +D + C + D +KR KEL++ K+
Sbjct: 456 ATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGSAKELLQHPFLKL 502
>sp|Q9ESL4|MLTK_MOUSE Mitogen-activated protein kinase kinase kinase MLT (MLK-like
mitogen-activated protein triple kinase) (Leucine zipper
and sterile alpha motif kinase ZAK) (Sterile-alpha motif
and leucine zipper containing kinase AZK) (Mixed lineage
kinase-related kinase MRK)
Length = 802
Score = 62.0 bits (149), Expect = 4e-10
Identities = 36/126 (28%), Positives = 61/126 (48%), Gaps = 5/126 (3%)
Frame = +2
Query: 11 SSSQSKVGVYSYMAPEVLNSEPYDNAADVWSFAAVLVNLITGKNPCPDKDPIDAAFHVAR 190
++ S VG + +MAPEV+ S P D +S+ VL ++T + P + + A+ V
Sbjct: 161 TTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVE 220
Query: 191 KLEMEYTLPEDTNQVLKDIITNCAQGDPKKRPKIKELIEI-----DLQKIPIQL*KFIHF 355
K E T+P + +++ C + D KKRP K++I I + +P Q F+H
Sbjct: 221 KNE-RLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTNLPDQCNSFLHN 279
Query: 356 LIRFSC 373
+ C
Sbjct: 280 KAEWRC 285
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,239,830
Number of Sequences: 369166
Number of extensions: 753156
Number of successful extensions: 3636
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3315
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3395
length of database: 68,354,980
effective HSP length: 97
effective length of database: 50,435,685
effective search space used: 1714813290
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)