Planarian EST Database


Dr_sW_009_L06

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_009_L06
         (496 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P37167|ACTP_ACACA  Actophorin                                   70   3e-12
sp|Q03048|COFI_YEAST  Cofilin                                      59   6e-09
sp|Q9LQ81|ADFX_ARATH  Actin-depolymerizing factor like At1g0...    59   8e-09
sp|P78929|COFI_SCHPO  Cofilin                                      57   2e-08
sp|Q9FVI1|ADF2_PETHY  Actin-depolymerizing factor 2 (ADF 2)        57   3e-08
sp|Q8SAG3|ADF_VITVI  Actin-depolymerizing factor (ADF)             57   3e-08
sp|P18359|DEST_CHICK  Destrin (Actin-depolymerizing factor) ...    56   4e-08
sp|P54706|COFI_DICDI  Cofilin                                      56   5e-08
sp|Q9ZSK2|ADF6_ARATH  Actin-depolymerizing factor 6 (ADF-6) ...    56   5e-08
sp|Q39250|ADF1_ARATH  Actin-depolymerizing factor 1 (ADF-1) ...    56   5e-08
>sp|P37167|ACTP_ACACA Actophorin
          Length = 138

 Score = 70.1 bits (170), Expect = 3e-12
 Identities = 37/134 (27%), Positives = 67/134 (50%), Gaps = 8/134 (5%)
 Frame = +2

Query: 11  DPSVEEYLNYIKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCY 190
           D  V+++ N +KL H+ +YV + +N     ++    G     YE FK  + E   +   Y
Sbjct: 8   DDCVQKF-NELKLGHQHRYVTFKMNASNTEVVVEHVGGPNATYEDFKSQLPERDCR---Y 63

Query: 191 ALYDYE--------GTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDE 346
           A++DYE          + FI W P++  I S+M+  ++K  I  ++VG++ +++A    E
Sbjct: 64  AIFDYEFQVDGGQRNKITFILWAPDSAPIKSKMMYTSTKDSIKKKLVGIQVEVQATDAAE 123

Query: 347 IDESVIKEKTASKV 388
           I E  + E+    V
Sbjct: 124 ISEDAVSERAKKDV 137
>sp|Q03048|COFI_YEAST Cofilin
          Length = 143

 Score = 58.9 bits (141), Expect = 6e-09
 Identities = 32/125 (25%), Positives = 61/125 (48%), Gaps = 10/125 (8%)
 Frame = +2

Query: 35  NYIKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYALYDYE-- 208
           N +KL  K++++++GLN  K  I+  ET      Y+ F + + E    +  YA+YD+E  
Sbjct: 17  NDLKLGKKYKFILFGLNDAKTEIVVKETST-DPSYDAFLEKLPEN---DCLYAIYDFEYE 72

Query: 209 --------GTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVI 364
                     + F +W P+T  + S+M+ A+SK  +   + GV   ++     E+    +
Sbjct: 73  INGNEGKRSKIVFFTWSPDTAPVRSKMVYASSKDALRRALNGVSTDVQGTDFSEVSYDSV 132

Query: 365 KEKTA 379
            E+ +
Sbjct: 133 LERVS 137
>sp|Q9LQ81|ADFX_ARATH Actin-depolymerizing factor like At1g01750 (ADF-like)
          Length = 140

 Score = 58.5 bits (140), Expect = 8e-09
 Identities = 29/118 (24%), Positives = 65/118 (55%), Gaps = 9/118 (7%)
 Frame = +2

Query: 41  IKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYALYDYEGT-- 214
           +K +  ++++++ ++++  +++  + G+    YE F + I E    E  YA+YDY+ T  
Sbjct: 21  LKAKRNYRFIVFKIDEKAQQVMIDKLGNPEETYEDFTRSIPE---DECRYAVYDYDFTTP 77

Query: 215 -------LAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVIK 367
                  + FI+W P+T ++ S+M+ A+SK      + G++ +++A    E+   +IK
Sbjct: 78  ENCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKRELDGIQVELQATDPSEMSLDIIK 135
>sp|P78929|COFI_SCHPO Cofilin
          Length = 137

 Score = 57.4 bits (137), Expect = 2e-08
 Identities = 37/136 (27%), Positives = 60/136 (44%), Gaps = 8/136 (5%)
 Frame = +2

Query: 2   VKCDPSVEEYLNYIKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQE 181
           VK  P   E    +KL    +YV++ +N  K  I+ +E       ++ F   + E   + 
Sbjct: 6   VKVSPECLEAFQELKLGKSLRYVVFKMNDTKTEIV-VEKKSTDKDFDTFLGDLPEKDCR- 63

Query: 182 GCYALYDYEGTLA--------FISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANS 337
             YA+YD+E  L         FISW P+   I S+M+ ++SK  +     G+   I+A  
Sbjct: 64  --YAIYDFEFNLGEGVRNKIIFISWSPDVAPIKSKMVYSSSKDTLRRAFTGIGTDIQATD 121

Query: 338 EDEIDESVIKEKTASK 385
             E+    + EK   K
Sbjct: 122 FSEVAYETVLEKVTRK 137
>sp|Q9FVI1|ADF2_PETHY Actin-depolymerizing factor 2 (ADF 2)
          Length = 143

 Score = 56.6 bits (135), Expect = 3e-08
 Identities = 28/120 (23%), Positives = 61/120 (50%), Gaps = 9/120 (7%)
 Frame = +2

Query: 41  IKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYALYDYE---- 208
           +K +  ++++IY + +++  ++      LG P E ++       A E  YA+YD++    
Sbjct: 21  LKAKRTYRFIIYKIEEKQKEVV---VEKLGEPTESYEDFTAGLPADECRYAVYDFDFMTK 77

Query: 209 -----GTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVIKEK 373
                  + FI+W P+T ++ S+MI A+SK      + G++ +++A    E+   V + +
Sbjct: 78  ENHQKSRIFFIAWSPDTARVRSKMIYASSKDRFKRELDGIQVELQATDPTEMGLDVFRSR 137
>sp|Q8SAG3|ADF_VITVI Actin-depolymerizing factor (ADF)
          Length = 143

 Score = 56.6 bits (135), Expect = 3e-08
 Identities = 30/120 (25%), Positives = 64/120 (53%), Gaps = 9/120 (7%)
 Frame = +2

Query: 41  IKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYALYDYE---- 208
           +K +   +YVI+ ++++K  ++  +TG     +++F   + E   +   YA+YD++    
Sbjct: 25  LKRKKVHRYVIFKIDEKKKEVVVEKTGGPAESFDEFAAALPENDCR---YAVYDFDFVTS 81

Query: 209 -----GTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVIKEK 373
                  + FI+W P++ +I ++M+ A SK      + GV  +I+A    E+D  V++E+
Sbjct: 82  ENCQKSKIFFIAWSPDSSRIRAKMLYATSKERFRRELDGVHYEIQATDPTEMDLEVLRER 141
>sp|P18359|DEST_CHICK Destrin (Actin-depolymerizing factor) (ADF)
          Length = 165

 Score = 56.2 bits (134), Expect = 4e-08
 Identities = 37/116 (31%), Positives = 58/116 (50%), Gaps = 14/116 (12%)
 Frame = +2

Query: 68  VIYGLNKEKNRIIKLE-----TGDLGTPYEKFKQHIIETYAQEGC-YALYDY-------- 205
           VI+ L+ +K  II  E      GD+G       +H +E   ++ C YALYD         
Sbjct: 36  VIFCLSPDKKCIIVEEGKEILVGDVGVTVTDPFKHFVEMLPEKDCRYALYDASFETKESK 95

Query: 206 EGTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVIKEK 373
           +  L F  W PE   + S+MI A+SK  I  +  G+K + +AN  ++++ + I EK
Sbjct: 96  KEELMFFLWAPEQAPLKSKMIYASSKDAIKKKFQGIKHECQANGPEDLNRACIAEK 151
>sp|P54706|COFI_DICDI Cofilin
          Length = 137

 Score = 55.8 bits (133), Expect = 5e-08
 Identities = 31/128 (24%), Positives = 60/128 (46%), Gaps = 8/128 (6%)
 Frame = +2

Query: 14  PSVEEYLNYIKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYA 193
           P+     N +KL  K+  +IY ++ +   II   T   G  +++F + + E   +   Y 
Sbjct: 9   PNCVSTFNDLKLGRKYGGIIYRISDDSKEIIVDSTLPAGCSFDEFTKCLPENECR---YV 65

Query: 194 LYDYE--------GTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEI 349
           + DY+          + F++W P+T  I  +M+  +SK  +    VG++ +I+     E+
Sbjct: 66  VLDYQYKEEGAQKSKICFVAWCPDTANIKKKMMATSSKDSLRKACVGIQVEIQGTDASEV 125

Query: 350 DESVIKEK 373
            +S   EK
Sbjct: 126 KDSCFYEK 133
>sp|Q9ZSK2|ADF6_ARATH Actin-depolymerizing factor 6 (ADF-6) (AtADF6)
          Length = 146

 Score = 55.8 bits (133), Expect = 5e-08
 Identities = 29/128 (22%), Positives = 67/128 (52%), Gaps = 11/128 (8%)
 Frame = +2

Query: 23  EEYLNYIKLQHK--FQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYAL 196
           E    +++LQ K   +YV++ +++ K  ++  +TG+    Y+ F   + +   +   YA+
Sbjct: 20  ESKTTFLELQRKKTHRYVVFKIDESKKEVVVEKTGNPTESYDDFLASLPDNDCR---YAV 76

Query: 197 YDYE---------GTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEI 349
           YD++           + F +W P T  I ++++ + SK +++  + G+  +I+A    E+
Sbjct: 77  YDFDFVTSENCQKSKIFFFAWSPSTSGIRAKVLYSTSKDQLSRELQGIHYEIQATDPTEV 136

Query: 350 DESVIKEK 373
           D  V++E+
Sbjct: 137 DLEVLRER 144
>sp|Q39250|ADF1_ARATH Actin-depolymerizing factor 1 (ADF-1) (AtADF1)
          Length = 139

 Score = 55.8 bits (133), Expect = 5e-08
 Identities = 29/120 (24%), Positives = 62/120 (51%), Gaps = 9/120 (7%)
 Frame = +2

Query: 41  IKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYALYDYEGTLA 220
           +K +   ++++Y + +++ +++  + G     YE+F   +    A E  YA+YD++   A
Sbjct: 21  LKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACL---PADECRYAIYDFDFVTA 77

Query: 221 ---------FISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVIKEK 373
                    FI+W P+  K+ S+MI A+SK      + G++ +++A    E+D  V + +
Sbjct: 78  ENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVELQATDPTEMDLDVFRSR 137
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 48,926,636
Number of Sequences: 369166
Number of extensions: 834930
Number of successful extensions: 1971
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1924
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1966
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 3069744620
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)