Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Dr_sW_009_L06 (496 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|P37167|ACTP_ACACA Actophorin 70 3e-12 sp|Q03048|COFI_YEAST Cofilin 59 6e-09 sp|Q9LQ81|ADFX_ARATH Actin-depolymerizing factor like At1g0... 59 8e-09 sp|P78929|COFI_SCHPO Cofilin 57 2e-08 sp|Q9FVI1|ADF2_PETHY Actin-depolymerizing factor 2 (ADF 2) 57 3e-08 sp|Q8SAG3|ADF_VITVI Actin-depolymerizing factor (ADF) 57 3e-08 sp|P18359|DEST_CHICK Destrin (Actin-depolymerizing factor) ... 56 4e-08 sp|P54706|COFI_DICDI Cofilin 56 5e-08 sp|Q9ZSK2|ADF6_ARATH Actin-depolymerizing factor 6 (ADF-6) ... 56 5e-08 sp|Q39250|ADF1_ARATH Actin-depolymerizing factor 1 (ADF-1) ... 56 5e-08
>sp|P37167|ACTP_ACACA Actophorin Length = 138 Score = 70.1 bits (170), Expect = 3e-12 Identities = 37/134 (27%), Positives = 67/134 (50%), Gaps = 8/134 (5%) Frame = +2 Query: 11 DPSVEEYLNYIKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCY 190 D V+++ N +KL H+ +YV + +N ++ G YE FK + E + Y Sbjct: 8 DDCVQKF-NELKLGHQHRYVTFKMNASNTEVVVEHVGGPNATYEDFKSQLPERDCR---Y 63 Query: 191 ALYDYE--------GTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDE 346 A++DYE + FI W P++ I S+M+ ++K I ++VG++ +++A E Sbjct: 64 AIFDYEFQVDGGQRNKITFILWAPDSAPIKSKMMYTSTKDSIKKKLVGIQVEVQATDAAE 123 Query: 347 IDESVIKEKTASKV 388 I E + E+ V Sbjct: 124 ISEDAVSERAKKDV 137
>sp|Q03048|COFI_YEAST Cofilin Length = 143 Score = 58.9 bits (141), Expect = 6e-09 Identities = 32/125 (25%), Positives = 61/125 (48%), Gaps = 10/125 (8%) Frame = +2 Query: 35 NYIKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYALYDYE-- 208 N +KL K++++++GLN K I+ ET Y+ F + + E + YA+YD+E Sbjct: 17 NDLKLGKKYKFILFGLNDAKTEIVVKETST-DPSYDAFLEKLPEN---DCLYAIYDFEYE 72 Query: 209 --------GTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVI 364 + F +W P+T + S+M+ A+SK + + GV ++ E+ + Sbjct: 73 INGNEGKRSKIVFFTWSPDTAPVRSKMVYASSKDALRRALNGVSTDVQGTDFSEVSYDSV 132 Query: 365 KEKTA 379 E+ + Sbjct: 133 LERVS 137
>sp|Q9LQ81|ADFX_ARATH Actin-depolymerizing factor like At1g01750 (ADF-like) Length = 140 Score = 58.5 bits (140), Expect = 8e-09 Identities = 29/118 (24%), Positives = 65/118 (55%), Gaps = 9/118 (7%) Frame = +2 Query: 41 IKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYALYDYEGT-- 214 +K + ++++++ ++++ +++ + G+ YE F + I E E YA+YDY+ T Sbjct: 21 LKAKRNYRFIVFKIDEKAQQVMIDKLGNPEETYEDFTRSIPE---DECRYAVYDYDFTTP 77 Query: 215 -------LAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVIK 367 + FI+W P+T ++ S+M+ A+SK + G++ +++A E+ +IK Sbjct: 78 ENCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKRELDGIQVELQATDPSEMSLDIIK 135
>sp|P78929|COFI_SCHPO Cofilin Length = 137 Score = 57.4 bits (137), Expect = 2e-08 Identities = 37/136 (27%), Positives = 60/136 (44%), Gaps = 8/136 (5%) Frame = +2 Query: 2 VKCDPSVEEYLNYIKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQE 181 VK P E +KL +YV++ +N K I+ +E ++ F + E + Sbjct: 6 VKVSPECLEAFQELKLGKSLRYVVFKMNDTKTEIV-VEKKSTDKDFDTFLGDLPEKDCR- 63 Query: 182 GCYALYDYEGTLA--------FISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANS 337 YA+YD+E L FISW P+ I S+M+ ++SK + G+ I+A Sbjct: 64 --YAIYDFEFNLGEGVRNKIIFISWSPDVAPIKSKMVYSSSKDTLRRAFTGIGTDIQATD 121 Query: 338 EDEIDESVIKEKTASK 385 E+ + EK K Sbjct: 122 FSEVAYETVLEKVTRK 137
>sp|Q9FVI1|ADF2_PETHY Actin-depolymerizing factor 2 (ADF 2) Length = 143 Score = 56.6 bits (135), Expect = 3e-08 Identities = 28/120 (23%), Positives = 61/120 (50%), Gaps = 9/120 (7%) Frame = +2 Query: 41 IKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYALYDYE---- 208 +K + ++++IY + +++ ++ LG P E ++ A E YA+YD++ Sbjct: 21 LKAKRTYRFIIYKIEEKQKEVV---VEKLGEPTESYEDFTAGLPADECRYAVYDFDFMTK 77 Query: 209 -----GTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVIKEK 373 + FI+W P+T ++ S+MI A+SK + G++ +++A E+ V + + Sbjct: 78 ENHQKSRIFFIAWSPDTARVRSKMIYASSKDRFKRELDGIQVELQATDPTEMGLDVFRSR 137
>sp|Q8SAG3|ADF_VITVI Actin-depolymerizing factor (ADF) Length = 143 Score = 56.6 bits (135), Expect = 3e-08 Identities = 30/120 (25%), Positives = 64/120 (53%), Gaps = 9/120 (7%) Frame = +2 Query: 41 IKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYALYDYE---- 208 +K + +YVI+ ++++K ++ +TG +++F + E + YA+YD++ Sbjct: 25 LKRKKVHRYVIFKIDEKKKEVVVEKTGGPAESFDEFAAALPENDCR---YAVYDFDFVTS 81 Query: 209 -----GTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVIKEK 373 + FI+W P++ +I ++M+ A SK + GV +I+A E+D V++E+ Sbjct: 82 ENCQKSKIFFIAWSPDSSRIRAKMLYATSKERFRRELDGVHYEIQATDPTEMDLEVLRER 141
>sp|P18359|DEST_CHICK Destrin (Actin-depolymerizing factor) (ADF) Length = 165 Score = 56.2 bits (134), Expect = 4e-08 Identities = 37/116 (31%), Positives = 58/116 (50%), Gaps = 14/116 (12%) Frame = +2 Query: 68 VIYGLNKEKNRIIKLE-----TGDLGTPYEKFKQHIIETYAQEGC-YALYDY-------- 205 VI+ L+ +K II E GD+G +H +E ++ C YALYD Sbjct: 36 VIFCLSPDKKCIIVEEGKEILVGDVGVTVTDPFKHFVEMLPEKDCRYALYDASFETKESK 95 Query: 206 EGTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVIKEK 373 + L F W PE + S+MI A+SK I + G+K + +AN ++++ + I EK Sbjct: 96 KEELMFFLWAPEQAPLKSKMIYASSKDAIKKKFQGIKHECQANGPEDLNRACIAEK 151
>sp|P54706|COFI_DICDI Cofilin Length = 137 Score = 55.8 bits (133), Expect = 5e-08 Identities = 31/128 (24%), Positives = 60/128 (46%), Gaps = 8/128 (6%) Frame = +2 Query: 14 PSVEEYLNYIKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYA 193 P+ N +KL K+ +IY ++ + II T G +++F + + E + Y Sbjct: 9 PNCVSTFNDLKLGRKYGGIIYRISDDSKEIIVDSTLPAGCSFDEFTKCLPENECR---YV 65 Query: 194 LYDYE--------GTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEI 349 + DY+ + F++W P+T I +M+ +SK + VG++ +I+ E+ Sbjct: 66 VLDYQYKEEGAQKSKICFVAWCPDTANIKKKMMATSSKDSLRKACVGIQVEIQGTDASEV 125 Query: 350 DESVIKEK 373 +S EK Sbjct: 126 KDSCFYEK 133
>sp|Q9ZSK2|ADF6_ARATH Actin-depolymerizing factor 6 (ADF-6) (AtADF6) Length = 146 Score = 55.8 bits (133), Expect = 5e-08 Identities = 29/128 (22%), Positives = 67/128 (52%), Gaps = 11/128 (8%) Frame = +2 Query: 23 EEYLNYIKLQHK--FQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYAL 196 E +++LQ K +YV++ +++ K ++ +TG+ Y+ F + + + YA+ Sbjct: 20 ESKTTFLELQRKKTHRYVVFKIDESKKEVVVEKTGNPTESYDDFLASLPDNDCR---YAV 76 Query: 197 YDYE---------GTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEI 349 YD++ + F +W P T I ++++ + SK +++ + G+ +I+A E+ Sbjct: 77 YDFDFVTSENCQKSKIFFFAWSPSTSGIRAKVLYSTSKDQLSRELQGIHYEIQATDPTEV 136 Query: 350 DESVIKEK 373 D V++E+ Sbjct: 137 DLEVLRER 144
>sp|Q39250|ADF1_ARATH Actin-depolymerizing factor 1 (ADF-1) (AtADF1) Length = 139 Score = 55.8 bits (133), Expect = 5e-08 Identities = 29/120 (24%), Positives = 62/120 (51%), Gaps = 9/120 (7%) Frame = +2 Query: 41 IKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYALYDYEGTLA 220 +K + ++++Y + +++ +++ + G YE+F + A E YA+YD++ A Sbjct: 21 LKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACL---PADECRYAIYDFDFVTA 77 Query: 221 ---------FISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVIKEK 373 FI+W P+ K+ S+MI A+SK + G++ +++A E+D V + + Sbjct: 78 ENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVELQATDPTEMDLDVFRSR 137
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 48,926,636 Number of Sequences: 369166 Number of extensions: 834930 Number of successful extensions: 1971 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 1924 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1966 length of database: 68,354,980 effective HSP length: 102 effective length of database: 49,512,010 effective search space used: 3069744620 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)