Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_009_L03
(560 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9LM02|SMT1_ARATH Cycloartenol-C-24-methyltransferase (2... 40 0.003
sp|Q96WX4|ERG6_PNECA Sterol 24-C-methyltransferase (Delta(2... 39 0.009
sp|Q6ZIX2|SMT1_ORYSA Cycloartenol-C-24-methyltransferase 1 ... 38 0.020
sp|O14321|ERG6_SCHPO Sterol 24-C-methyltransferase (Delta(2... 36 0.057
sp|Q71Y84|UBIE_LISMF Menaquinone biosynthesis methyltransfe... 36 0.074
sp|Q8CSH9|UBIE_STAES Menaquinone biosynthesis methyltransfe... 35 0.13
sp|Q9RRT0|UBIE_DEIRA Menaquinone biosynthesis methyltransfe... 34 0.22
sp|P46326|YXBB_BACSU Hypothetical protein yxbB 33 0.48
sp|O74198|ERG6_CANAL Sterol 24-C-methyltransferase (Delta(2... 33 0.63
sp|Q6FRZ7|ERG6_CANGA Sterol 24-C-methyltransferase (Delta(2... 33 0.63
>sp|Q9LM02|SMT1_ARATH Cycloartenol-C-24-methyltransferase (24-sterol C-methyltransferase
1) (Sterol C-methyltransferase 1) (STEROL
METHYLTRANSFERASE 1) (CEPHALOPOD protein)
Length = 336
Score = 40.4 bits (93), Expect = 0.003
Identities = 32/99 (32%), Positives = 48/99 (48%), Gaps = 1/99 (1%)
Frame = +1
Query: 10 GFGVGYGLSKVVKIINEGKGKIYGTEMSDYCIQKFYKYLNKMDVNHKTIKLYKADVSDLP 189
G G+G L ++ + N + G ++Y I + K LN++ KT KAD +P
Sbjct: 102 GCGIGGPLREIARFSNS---VVTGLNNNEYQITRG-KELNRLAGVDKTCNFVKADFMKMP 157
Query: 190 IMSNSISVVYHVNCFYSWNNPIQSARGCL-EMYRVLRPG 303
NS VY + + + P A GC E+YRVL+PG
Sbjct: 158 FPENSFDAVYAIEA--TCHAP--DAYGCYKEIYRVLKPG 192
>sp|Q96WX4|ERG6_PNECA Sterol 24-C-methyltransferase (Delta(24)-sterol
C-methyltransferase)
Length = 377
Score = 38.9 bits (89), Expect = 0.009
Identities = 30/99 (30%), Positives = 47/99 (47%), Gaps = 1/99 (1%)
Frame = +1
Query: 73 IYGTEMSDYCIQKFYKYLNKMDVNHKTIKLYKADVSDLPIMSNSISVVYHVNCFYSWNNP 252
I G +DY IQ+ Y K ++ K +K K D +P NS +Y +
Sbjct: 152 IVGLNNNDYQIQRAKYYSEKKGLSDK-LKFIKGDFMQMPFPENSFDKIYSIEATIH---- 206
Query: 253 IQSARGCL-EMYRVLRPGGQVVSTFNKSMFKKIFNQNIP 366
S G E+YRVL+PGG + +++ M K +++N P
Sbjct: 207 APSLEGVYSEIYRVLKPGG-LYASYEWVMLNK-YDENDP 243
>sp|Q6ZIX2|SMT1_ORYSA Cycloartenol-C-24-methyltransferase 1 (24-sterol
C-methyltransferase 1) (Sterol C-methyltransferase 1)
Length = 344
Score = 37.7 bits (86), Expect = 0.020
Identities = 29/99 (29%), Positives = 45/99 (45%), Gaps = 1/99 (1%)
Frame = +1
Query: 10 GFGVGYGLSKVVKIINEGKGKIYGTEMSDYCIQKFYKYLNKMDVNHKTIKLYKADVSDLP 189
G G+G L ++ K + G ++Y I + K LN++ T KAD +P
Sbjct: 108 GCGIGGPLREIAKF---SLASVTGLNNNEYQITRG-KELNRVAGVSGTCDFVKADFMKMP 163
Query: 190 IMSNSISVVYHVNCFYSWNNPIQSARGCL-EMYRVLRPG 303
N+ VY + +P+ GC E+YRVL+PG
Sbjct: 164 FSDNTFDAVYAIEATCHAPDPV----GCYKEIYRVLKPG 198
>sp|O14321|ERG6_SCHPO Sterol 24-C-methyltransferase (Delta(24)-sterol
C-methyltransferase)
Length = 378
Score = 36.2 bits (82), Expect = 0.057
Identities = 28/102 (27%), Positives = 47/102 (46%), Gaps = 1/102 (0%)
Frame = +1
Query: 4 ARGFGVGYGLSKVVKIINEGKG-KIYGTEMSDYCIQKFYKYLNKMDVNHKTIKLYKADVS 180
+R VG G+ + I E G + G +DY I + Y K +++ K + K D
Sbjct: 126 SRVLDVGCGVGGPAREITEFTGCNLVGLNNNDYQISRCNNYAVKRNLDKKQV-FVKGDFM 184
Query: 181 DLPIMSNSISVVYHVNCFYSWNNPIQSARGCLEMYRVLRPGG 306
+P N+ VY + + ++ G E++RVL+PGG
Sbjct: 185 HMPFEDNTFDYVYAIEATVHAPS-LEGVYG--EIFRVLKPGG 223
>sp|Q71Y84|UBIE_LISMF Menaquinone biosynthesis methyltransferase ubiE
sp|P67056|UBIE_LISIN Menaquinone biosynthesis methyltransferase ubiE
sp|P67055|UBIE_LISMO Menaquinone biosynthesis methyltransferase ubiE
Length = 237
Score = 35.8 bits (81), Expect = 0.074
Identities = 27/110 (24%), Positives = 53/110 (48%), Gaps = 4/110 (3%)
Frame = +1
Query: 64 KGKIYGTEMSDYCIQKFYKYLNKMDVNHKTIKLYKADVSDLPIMSNSISVVYHVNCFYSW 243
+G + G + S+ ++ + + + D+++ ++L + +LP NS +V +
Sbjct: 72 EGHVTGLDFSENMLKVGREKVKEADLHN--VELIHGNAMELPFPDNSFD---YVTIGFGL 126
Query: 244 NNPIQSARGCLEMYRVLRPGGQV----VSTFNKSMFKKIFNQNIPILNPL 381
N + EMYRVL+PGGQ+ S N +K++FN + P+
Sbjct: 127 RNVPDYMQVLREMYRVLKPGGQLACIDTSQPNIPGWKQVFNAYFRYVMPV 176
>sp|Q8CSH9|UBIE_STAES Menaquinone biosynthesis methyltransferase ubiE
sp|Q5HP74|UBIE_STAEQ Menaquinone biosynthesis methyltransferase ubiE
Length = 241
Score = 35.0 bits (79), Expect = 0.13
Identities = 28/110 (25%), Positives = 49/110 (44%), Gaps = 4/110 (3%)
Frame = +1
Query: 64 KGKIYGTEMSDYCIQKFYKYLNKMDVNHKTIKLYKADVSDLPIMSNSISVVYHVNCFYSW 243
KG++ G + S+ ++ + ++ I+L D +LP NS +V +
Sbjct: 74 KGQVTGLDFSENMLEVGKQKTASLE----NIQLVHGDAMNLPFDDNSFD---YVTIGFGL 126
Query: 244 NNPIQSARGCLEMYRVLRPGGQVV----STFNKSMFKKIFNQNIPILNPL 381
N EM+RVL+PGG VV S +FK+I++ + P+
Sbjct: 127 RNVPDYLSALKEMHRVLKPGGMVVCLETSQPTLPLFKQIYSLYFKFVMPI 176
>sp|Q9RRT0|UBIE_DEIRA Menaquinone biosynthesis methyltransferase ubiE
Length = 241
Score = 34.3 bits (77), Expect = 0.22
Identities = 22/75 (29%), Positives = 32/75 (42%)
Frame = +1
Query: 91 SDYCIQKFYKYLNKMDVNHKTIKLYKADVSDLPIMSNSISVVYHVNCFYSWNNPIQSARG 270
SD+ Q K +I+ + D LP S V C + + N +G
Sbjct: 87 SDFVPQMLDLARQKAGAKQLSIRFEEGDALRLPYPDASFDAV---TCAFGFRNFADYTQG 143
Query: 271 CLEMYRVLRPGGQVV 315
EM+RVL PGG++V
Sbjct: 144 LAEMWRVLTPGGRLV 158
>sp|P46326|YXBB_BACSU Hypothetical protein yxbB
Length = 244
Score = 33.1 bits (74), Expect = 0.48
Identities = 17/54 (31%), Positives = 26/54 (48%), Gaps = 2/54 (3%)
Frame = +1
Query: 172 DVSDLPIMSNSISVVYHVNCFYSWNNPIQSARGCLEMYRVLRPGGQVV--STFN 327
DV L + +C + W + ++ + C YRVL PGG++V TFN
Sbjct: 94 DVHHLSYADQYADFIVSYSCLHHWEDVVKGLKEC---YRVLAPGGKIVILDTFN 144
>sp|O74198|ERG6_CANAL Sterol 24-C-methyltransferase (Delta(24)-sterol
C-methyltransferase)
Length = 376
Score = 32.7 bits (73), Expect = 0.63
Identities = 22/79 (27%), Positives = 37/79 (46%)
Frame = +1
Query: 70 KIYGTEMSDYCIQKFYKYLNKMDVNHKTIKLYKADVSDLPIMSNSISVVYHVNCFYSWNN 249
+I G +DY I++ Y K ++HK + K D + S VY + + +
Sbjct: 147 EIVGLNNNDYQIERANHYAKKYHLDHK-LSYVKGDFMQMDFEPESFDAVYAIEA--TVHA 203
Query: 250 PIQSARGCLEMYRVLRPGG 306
P+ E+Y+VL+PGG
Sbjct: 204 PVLEG-VYSEIYKVLKPGG 221
>sp|Q6FRZ7|ERG6_CANGA Sterol 24-C-methyltransferase (Delta(24)-sterol
C-methyltransferase)
Length = 372
Score = 32.7 bits (73), Expect = 0.63
Identities = 29/98 (29%), Positives = 45/98 (45%), Gaps = 2/98 (2%)
Frame = +1
Query: 19 VGYGLSKVVKIINEGKG-KIYGTEMSDYCIQKFYKYLNKMDVNHKTIKLYKADVSDLPIM 195
VG G+ + I G I G +DY IQK Y K ++ + K D ++
Sbjct: 126 VGCGVGGPARTIARFTGCNIIGLNNNDYQIQKAKYYAKKYNLQDH-MDFVKGDFMNMEFE 184
Query: 196 SNSISVVYHVNCFYSWNNP-IQSARGCLEMYRVLRPGG 306
NS VY + + + P ++ G E+Y+VL+PGG
Sbjct: 185 PNSFDKVYAIEA--TCHAPKLEGVYG--EIYKVLKPGG 218
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 60,586,636
Number of Sequences: 369166
Number of extensions: 1180303
Number of successful extensions: 3125
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3040
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3121
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4029688280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)