Planarian EST Database


Dr_sW_009_K13

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_009_K13
         (447 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q05052|OST48_CANFA  Dolichyl-diphosphooligosaccharide--pr...   171   7e-43
sp|O54734|OST48_MOUSE  Dolichyl-diphosphooligosaccharide--pr...   170   1e-42
sp|P48440|OST48_CHICK  Dolichyl-diphosphooligosaccharide--pr...   169   2e-42
sp|P39656|OST48_HUMAN  Dolichyl-diphosphooligosaccharide--pr...   167   1e-41
sp|P45971|OST48_CAEEL  Probable dolichyl-diphosphooligosacch...   159   3e-39
sp|Q24319|OST48_DROME  Dolichyl-diphosphooligosaccharide--pr...   154   9e-38
sp|P33767|OSTB_YEAST  Dolichyl-diphosphooligosaccharide--pro...    61   1e-09
sp|O78432|YCF30_GUITH  Putative HTH-type transcriptional reg...    33   0.29 
sp|Q4IR18|GRC3_GIBZE  Protein GRC3                                 30   2.4  
sp|Q15911|ATBF1_HUMAN  Alpha-fetoprotein enhancer binding pr...    30   3.2  
>sp|Q05052|OST48_CANFA Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
           kDa subunit precursor (Oligosaccharyl transferase 48 kDa
           subunit)
          Length = 445

 Score =  171 bits (433), Expect = 7e-43
 Identities = 82/124 (66%), Positives = 104/124 (83%)
 Frame = +1

Query: 10  IVIDELRADGTWTPFIADDVQLEFYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFLVD 189
           IVI++L ++G W PF  DD+QLEF RIDPFVR  + +K GK+ + FKLPDVYGVF+F VD
Sbjct: 324 IVIEQL-SNGRWVPFDGDDIQLEFVRIDPFVRTFLKKKGGKYSVQFKLPDVYGVFQFKVD 382

Query: 190 YHRVGYTHVHSSTQVSVRPLMHTQYERFIQAAYPYYASAISMMVGVILFSFLFLYFKDDK 369
           Y+R+GYTH+HSSTQVSVRPL HTQYERFI +AYPYYASA SMM+G+ +FS +FL+ K +K
Sbjct: 383 YNRLGYTHLHSSTQVSVRPLQHTQYERFIPSAYPYYASAFSMMLGLFIFSTVFLHMK-EK 441

Query: 370 EKNE 381
           EK++
Sbjct: 442 EKSD 445
>sp|O54734|OST48_MOUSE Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
           kDa subunit precursor (Oligosaccharyl transferase 48 kDa
           subunit) (DDOST 48 kDa subunit)
          Length = 441

 Score =  170 bits (431), Expect = 1e-42
 Identities = 82/124 (66%), Positives = 103/124 (83%)
 Frame = +1

Query: 10  IVIDELRADGTWTPFIADDVQLEFYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFLVD 189
           IVI++L ++G W PF  DD+QLEF RIDPFVR  + RK GK+ + FKLPDVYGVF+F VD
Sbjct: 320 IVIEQL-SNGKWVPFDGDDIQLEFVRIDPFVRTFLKRKGGKYSVQFKLPDVYGVFQFKVD 378

Query: 190 YHRVGYTHVHSSTQVSVRPLMHTQYERFIQAAYPYYASAISMMVGVILFSFLFLYFKDDK 369
           Y+R+GYTH++SSTQVSVRPL HTQYERFI +AYPYYASA SMM G+ +FS +FL+ K +K
Sbjct: 379 YNRLGYTHLYSSTQVSVRPLQHTQYERFIPSAYPYYASAFSMMAGLFIFSIVFLHMK-EK 437

Query: 370 EKNE 381
           EK++
Sbjct: 438 EKSD 441
>sp|P48440|OST48_CHICK Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
           kDa subunit (Oligosaccharyl transferase 48 kDa subunit)
           (OST 50 kDa subunit) (DDOST 48 kDa subunit)
          Length = 413

 Score =  169 bits (429), Expect = 2e-42
 Identities = 82/124 (66%), Positives = 102/124 (82%)
 Frame = +1

Query: 10  IVIDELRADGTWTPFIADDVQLEFYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFLVD 189
           IVI++L +DG W PF  DD+QLEF RIDPFVR  + R  GK+ + FKLPDVYGVF+F VD
Sbjct: 292 IVIEKL-SDGKWIPFDGDDIQLEFVRIDPFVRTFLKRNGGKYSVQFKLPDVYGVFQFKVD 350

Query: 190 YHRVGYTHVHSSTQVSVRPLMHTQYERFIQAAYPYYASAISMMVGVILFSFLFLYFKDDK 369
           Y+R+GYTH++SSTQVSVRPL HTQYERFI +AYPYYA A SMMVG+ +FS +FL+ K +K
Sbjct: 351 YNRLGYTHLYSSTQVSVRPLQHTQYERFIPSAYPYYAGAFSMMVGLFMFSIVFLHMK-EK 409

Query: 370 EKNE 381
           EK++
Sbjct: 410 EKSD 413
>sp|P39656|OST48_HUMAN Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
           kDa subunit precursor (Oligosaccharyl transferase 48 kDa
           subunit) (DDOST 48 kDa subunit)
          Length = 439

 Score =  167 bits (422), Expect = 1e-41
 Identities = 80/124 (64%), Positives = 102/124 (82%)
 Frame = +1

Query: 10  IVIDELRADGTWTPFIADDVQLEFYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFLVD 189
           IVI +L ++G W PF  DD+QLEF RIDPFVR  + +K GK+ + FKLPDVYGVF+F VD
Sbjct: 318 IVIQQL-SNGKWVPFDGDDIQLEFVRIDPFVRTFLKKKGGKYSVQFKLPDVYGVFQFKVD 376

Query: 190 YHRVGYTHVHSSTQVSVRPLMHTQYERFIQAAYPYYASAISMMVGVILFSFLFLYFKDDK 369
           Y+R+GYTH++SSTQVSVRPL HTQYERFI +AYPYYASA  MM+G+ +FS +FL+ K +K
Sbjct: 377 YNRLGYTHLYSSTQVSVRPLQHTQYERFIPSAYPYYASAFPMMLGLFIFSIVFLHMK-EK 435

Query: 370 EKNE 381
           EK++
Sbjct: 436 EKSD 439
>sp|P45971|OST48_CAEEL Probable dolichyl-diphosphooligosaccharide--protein
           glycosyltransferase 48 kDa subunit precursor
           (Oligosaccharyl transferase 48 kDa subunit)
          Length = 445

 Score =  159 bits (401), Expect = 3e-39
 Identities = 77/122 (63%), Positives = 92/122 (75%)
 Frame = +1

Query: 10  IVIDELRADGTWTPFIADDVQLEFYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFLVD 189
           I I+EL+ +G W PF   DVQLEF RIDPFVR  +    G+  + FKLPDV GVFKFLVD
Sbjct: 313 IEIEELK-NGKWVPFQGKDVQLEFVRIDPFVRATLKNSNGRLSVAFKLPDVLGVFKFLVD 371

Query: 190 YHRVGYTHVHSSTQVSVRPLMHTQYERFIQAAYPYYASAISMMVGVILFSFLFLYFKDDK 369
           Y RVGYTH++   QVSVRPL HTQYERFI++AYPYYAS+ SMM G++LFS ++LY KD  
Sbjct: 372 YRRVGYTHLYDVQQVSVRPLWHTQYERFIRSAYPYYASSFSMMAGLVLFSIVYLYHKDTP 431

Query: 370 EK 375
            K
Sbjct: 432 VK 433
>sp|Q24319|OST48_DROME Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
           kDa subunit precursor (Oligosaccharyl transferase 48 kDa
           subunit) (DDOST 48 kDa subunit) (OST5OP) (DrOST)
           (DmOST50)
          Length = 449

 Score =  154 bits (389), Expect = 9e-38
 Identities = 75/120 (62%), Positives = 94/120 (78%), Gaps = 1/120 (0%)
 Frame = +1

Query: 10  IVIDELRADGTWTPFIADDVQLEFYRIDPFVRQNMLRKE-GKFYLDFKLPDVYGVFKFLV 186
           I I+EL   G W  F A D+QLEF RIDPFVR  + +   G +   FK+PDVYGV++F V
Sbjct: 318 IGIEEL-VQGEWRAFKASDIQLEFVRIDPFVRTYLKQTNTGAYQAKFKIPDVYGVYQFKV 376

Query: 187 DYHRVGYTHVHSSTQVSVRPLMHTQYERFIQAAYPYYASAISMMVGVILFSFLFLYFKDD 366
           DY+RVGYTH++S+TQVSVRPL HTQYERFI +A+PYY SA SMM+GV +FSF+FL+FKD+
Sbjct: 377 DYNRVGYTHLYSTTQVSVRPLEHTQYERFIPSAFPYYTSAFSMMIGVFVFSFVFLHFKDE 436
>sp|P33767|OSTB_YEAST Dolichyl-diphosphooligosaccharide--protein glycosyltransferase beta
           subunit precursor (Oligosaccharyl transferase beta
           subunit)
          Length = 430

 Score = 60.8 bits (146), Expect = 1e-09
 Identities = 35/111 (31%), Positives = 58/111 (52%), Gaps = 8/111 (7%)
 Frame = +1

Query: 43  WTPFIADDVQLEFYRIDPFVRQNMLRK----EGKFYL--DFKLPDVYGVFKFLVDYHRVG 204
           W P IADD+Q E  ++DP+ R  +       E ++Y   +F LPD +GVF FL DY ++G
Sbjct: 304 WLPHIADDIQFELRQVDPYYRLTLSPSGNDSETQYYTTGEFILPDRHGVFTFLTDYRKIG 363

Query: 205 YTHVHSSTQVSVRPLMHTQYERF--IQAAYPYYASAISMMVGVILFSFLFL 351
            +        ++R L + +Y R   I  ++ Y ++   ++V  I F   F+
Sbjct: 364 LSFTTDKDVKAIRHLANDEYPRSWEISNSWVYISAICGVIVAWIFFVVSFV 414
>sp|O78432|YCF30_GUITH Putative HTH-type transcriptional regulator ycf30
          Length = 310

 Score = 33.1 bits (74), Expect = 0.29
 Identities = 17/59 (28%), Positives = 32/59 (54%)
 Frame = +1

Query: 13  VIDELRADGTWTPFIADDVQLEFYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFLVD 189
           V DELR+    TP++ D++ L   ++ PF +  +++KE  + L F   D     + ++D
Sbjct: 155 VPDELRSKLQITPYVDDELALILPKLHPFSKLEVIQKEDLYRLRFITLDKQSTIRRVID 213
>sp|Q4IR18|GRC3_GIBZE Protein GRC3
          Length = 796

 Score = 30.0 bits (66), Expect = 2.4
 Identities = 21/72 (29%), Positives = 33/72 (45%)
 Frame = -1

Query: 366 VILEVEEQETEQNDSDHHGDGARVVGISSLDEALVLRVHQGPHRHLGGXXXXXXXXXXXV 187
           ++LEV E++TE + S    +GA   G+S L E   + V +G  R   G            
Sbjct: 658 MVLEVTEEDTEDDQSGVEPEGAD--GVSDLTEVPWVEVLKGSQRRPVGSQVWRPLRHLGR 715

Query: 186 NEELEHAVHVGQ 151
           N   ++ V VG+
Sbjct: 716 NNTGDYEVKVGE 727
>sp|Q15911|ATBF1_HUMAN Alpha-fetoprotein enhancer binding protein (AT motif-binding
           factor) (AT-binding transcription factor 1)
          Length = 3703

 Score = 29.6 bits (65), Expect = 3.2
 Identities = 14/39 (35%), Positives = 22/39 (56%)
 Frame = -1

Query: 357 EVEEQETEQNDSDHHGDGARVVGISSLDEALVLRVHQGP 241
           E EE+E E+ + +   +G + +  S LDE L  R H+ P
Sbjct: 473 EEEEEEEEEEEEEEEDEGCKGLFPSELDEELEDRPHEEP 511
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 47,692,952
Number of Sequences: 369166
Number of extensions: 881793
Number of successful extensions: 2715
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2654
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2708
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2394311040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)