Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_009_J22
(512 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9BS26|TXND4_HUMAN Thioredoxin domain-containing protein... 115 5e-26
sp|Q9D1Q6|TXND4_MOUSE Thioredoxin domain-containing protein... 113 2e-25
sp|P38658|ERP60_SCHMA Probable protein disulfide-isomerase ... 67 3e-11
sp|Q43116|PDI_RICCO Protein disulfide-isomerase precursor (... 66 6e-11
sp|Q9XI01|PDI1_ARATH Probable protein disulfide-isomerase 1... 65 1e-10
sp|P29828|PDI_MEDSA Protein disulfide-isomerase precursor (... 64 2e-10
sp|P80284|PDI_HORVU Protein disulfide-isomerase precursor (... 64 2e-10
sp|P32474|EUG1_YEAST Protein disulfide-isomerase EUG1 precu... 64 2e-10
sp|P52589|PDI_WHEAT Protein disulfide-isomerase precursor (... 64 2e-10
sp|Q17967|PDI1_CAEEL Protein disulfide-isomerase 1 precurso... 62 8e-10
>sp|Q9BS26|TXND4_HUMAN Thioredoxin domain-containing protein 4 precursor (Endoplasmic
reticulum resident protein ERp44)
Length = 406
Score = 115 bits (289), Expect = 5e-26
Identities = 61/152 (40%), Positives = 88/152 (57%), Gaps = 1/152 (0%)
Frame = +1
Query: 58 SFIYILTLVLSVCNCTVIDLTSTNIKTVLDSNDLVLIDFYASWCYFSNILDPIFTEASDL 237
S + ++T V + + L + NI +L++ D+ L++FYA WC FS +L PIF EASD+
Sbjct: 15 SLLLLVTWVFTPVTTEITSLDTENIDEILNNADVALVNFYADWCRFSQMLHPIFEEASDV 74
Query: 238 IAAEFXXXXXXXXXXXXCERQSDLSKQYGIRKYPTIKLFRYGEVDKHEYRGARSGPAFVE 417
I EF C++ SD++++Y I KYPT+KLFR G + K EYRG RS A +
Sbjct: 75 IKEEFPNENQVVFARVDCDQHSDIAQRYRISKYPTLKLFRNGMMMKREYRGQRSVKALAD 134
Query: 418 YIRSQLIDPIIYAKP-NEVIVRELGKSYIIGH 510
YIR Q DPI + E+ + K IIG+
Sbjct: 135 YIRQQKSDPIQEIRDLAEITTLDRSKRNIIGY 166
>sp|Q9D1Q6|TXND4_MOUSE Thioredoxin domain-containing protein 4 precursor (Endoplasmic
reticulum resident protein ERp44)
Length = 406
Score = 113 bits (283), Expect = 2e-25
Identities = 59/152 (38%), Positives = 89/152 (58%), Gaps = 1/152 (0%)
Frame = +1
Query: 58 SFIYILTLVLSVCNCTVIDLTSTNIKTVLDSNDLVLIDFYASWCYFSNILDPIFTEASDL 237
S + ++T + + + L S NI +L++ D+ L++FYA WC FS +L PIF EASD+
Sbjct: 15 SLLLLVTSIFTPITAEIASLDSENIDEILNNADVALVNFYADWCRFSQMLHPIFEEASDV 74
Query: 238 IAAEFXXXXXXXXXXXXCERQSDLSKQYGIRKYPTIKLFRYGEVDKHEYRGARSGPAFVE 417
I E+ C++ SD++++Y I KYPT+KLFR G + K EYRG RS A +
Sbjct: 75 IKEEYPDKNQVVFARVDCDQHSDIAQRYRISKYPTLKLFRNGMMMKREYRGQRSVKALAD 134
Query: 418 YIRSQLIDPIIYAKP-NEVIVRELGKSYIIGH 510
YIR Q +P+ + +EV + K IIG+
Sbjct: 135 YIRQQKSNPVHEIQSLDEVTNLDRSKRNIIGY 166
>sp|P38658|ERP60_SCHMA Probable protein disulfide-isomerase ER-60 precursor (ERP60)
Length = 484
Score = 66.6 bits (161), Expect = 3e-11
Identities = 39/127 (30%), Positives = 61/127 (48%)
Frame = +1
Query: 52 MKSFIYILTLVLSVCNCTVIDLTSTNIKTVLDSNDLVLIDFYASWCYFSNILDPIFTEAS 231
M+ + L LV V++LT N + L S + L+ FYA WC L P FT A+
Sbjct: 1 MRWLLSCLFLVAFASCSKVLELTKDNFHSELKSIPVALVKFYAPWCGHCKKLAPEFTSAA 60
Query: 232 DLIAAEFXXXXXXXXXXXXCERQSDLSKQYGIRKYPTIKLFRYGEVDKHEYRGARSGPAF 411
+I+ + C Q + ++G+ YPT+K+FR G++D EY G R+
Sbjct: 61 QIISGK---TNDVKLVKVDCTTQESICSEFGVSGYPTLKIFRNGDLD-GEYNGPRNANGI 116
Query: 412 VEYIRSQ 432
Y+ S+
Sbjct: 117 ANYMISR 123
Score = 32.3 bits (72), Expect = 0.67
Identities = 24/114 (21%), Positives = 47/114 (41%), Gaps = 2/114 (1%)
Frame = +1
Query: 115 LTSTNIKTVLDSNDL-VLIDFYASWCYFSNILDPIFTEASDLIAAEFXXXXXXXXXXXXC 291
L + N ++++ + V++ F+A WC L P + EA+ + E
Sbjct: 363 LVALNFDEIVNNEEKDVMVVFHAGWCGHCKNLMPKYEEAASKVKNE-----PNLVLAAMD 417
Query: 292 ERQSDLSKQYGIRKYPTIKLFRYGEVDKH-EYRGARSGPAFVEYIRSQLIDPII 450
+D+ Y +R +PTI G+ Y G R ++Y+ + + +I
Sbjct: 418 ATANDVPSPYQVRGFPTIYFVPKGKKSSPVSYEGGRDTNDIIKYLAREATEELI 471
>sp|Q43116|PDI_RICCO Protein disulfide-isomerase precursor (PDI)
Length = 498
Score = 65.9 bits (159), Expect = 6e-11
Identities = 37/137 (27%), Positives = 64/137 (46%), Gaps = 2/137 (1%)
Frame = +1
Query: 103 TVIDLTSTNIKTVLDSNDLVLIDFYASWCYFSNILDPIFTEASDLIAAEFXXXXXXXXXX 282
+V+ L STN + +D ++++FYA WC L P + +A+ ++ +
Sbjct: 33 SVLTLDSTNFTDTISKHDFIVVEFYAPWCGHCKKLRPEYEKAASILKSHDIPVVLAKVDA 92
Query: 283 XXCERQSDLSKQYGIRKYPTIKLFRYGEVDKHEYRGARSGPAFVEYIRSQLIDPIIYAKP 462
E +L+ QY I+ +PT+K+ R G EY+G R EY++ Q + K
Sbjct: 93 NE-EANKELATQYDIKGFPTLKILRNGGKSIQEYKGpreADGIAEYLKKQSGPASVEIKS 151
Query: 463 NEVIVRELG--KSYIIG 507
E +G K +I+G
Sbjct: 152 TEAANTFIGDKKIFIVG 168
Score = 35.8 bits (81), Expect = 0.061
Identities = 25/95 (26%), Positives = 37/95 (38%)
Frame = +1
Query: 139 VLDSNDLVLIDFYASWCYFSNILDPIFTEASDLIAAEFXXXXXXXXXXXXCERQSDLSKQ 318
V +S VL++FYA WC L PI E +A + S
Sbjct: 390 VFNSGKNVLLEFYAPWCGHCKQLAPILDE----VAVSYKSDADIVIAKLDATANDIPSDT 445
Query: 319 YGIRKYPTIKLFRYGEVDKHEYRGARSGPAFVEYI 423
+ +R YPT+ FR +Y G R+ + +I
Sbjct: 446 FDVRGYPTV-YFRSASGKVEQYDGDRTKDDIISFI 479
>sp|Q9XI01|PDI1_ARATH Probable protein disulfide-isomerase 1 precursor (PDI 1)
Length = 501
Score = 64.7 bits (156), Expect = 1e-10
Identities = 37/140 (26%), Positives = 63/140 (45%), Gaps = 11/140 (7%)
Frame = +1
Query: 46 LLMKSFIYILTLVLSVCNCT-----------VIDLTSTNIKTVLDSNDLVLIDFYASWCY 192
+ M+ F LVLS+C + V+ L TN ++ +D ++++FYA WC
Sbjct: 1 MAMRGFTLFSILVLSLCASSIRSEETETKEFVLTLDHTNFTDTINKHDFIVVEFYAPWCG 60
Query: 193 FSNILDPIFTEASDLIAAEFXXXXXXXXXXXXCERQSDLSKQYGIRKYPTIKLFRYGEVD 372
L P + +A+ +++ E + + QY ++ +PTIK+FR G
Sbjct: 61 HCKQLAPEYEKAASALSSNVPPVVLAKIDASE-ETNREFATQYEVQGFPTIKIFRNGGKA 119
Query: 373 KHEYRGARSGPAFVEYIRSQ 432
EY G R V Y++ Q
Sbjct: 120 VQEYNGpreAEGIVTYLKKQ 139
Score = 33.1 bits (74), Expect = 0.40
Identities = 23/95 (24%), Positives = 38/95 (40%)
Frame = +1
Query: 139 VLDSNDLVLIDFYASWCYFSNILDPIFTEASDLIAAEFXXXXXXXXXXXXCERQSDLSKQ 318
VL+S VL++FYA WC L PI E +A +
Sbjct: 388 VLNSGKNVLLEFYAPWCGHCQKLAPILDE----VAVSYQSDSSVVIAKLDATANDFPKDT 443
Query: 319 YGIRKYPTIKLFRYGEVDKHEYRGARSGPAFVEYI 423
+ ++ +PTI F+ + Y G R+ F+ ++
Sbjct: 444 FDVKGFPTI-YFKSASGNVVVYEGDRTKEDFISFV 477
>sp|P29828|PDI_MEDSA Protein disulfide-isomerase precursor (PDI)
Length = 512
Score = 64.3 bits (155), Expect = 2e-10
Identities = 31/109 (28%), Positives = 55/109 (50%)
Frame = +1
Query: 106 VIDLTSTNIKTVLDSNDLVLIDFYASWCYFSNILDPIFTEASDLIAAEFXXXXXXXXXXX 285
V+ L +TN + +D ++++FYA WC L P + +A+ +++
Sbjct: 35 VLTLDNTNFHDTVKKHDFIVVEFYAPWCGHCKKLAPEYEKAASILSTHEPPVVLAKVDAN 94
Query: 286 XCERQSDLSKQYGIRKYPTIKLFRYGEVDKHEYRGARSGPAFVEYIRSQ 432
E DL+ + ++ +PTIK+FR G + EY+G R VEY++ Q
Sbjct: 95 E-EHNKDLASENDVKGFPTIKIFRNGGKNIQEYKGpreAEGIVEYLKKQ 142
Score = 36.2 bits (82), Expect = 0.047
Identities = 26/95 (27%), Positives = 37/95 (38%)
Frame = +1
Query: 139 VLDSNDLVLIDFYASWCYFSNILDPIFTEASDLIAAEFXXXXXXXXXXXXCERQSDLSKQ 318
V S VLI+FYA WC L PI E +A F +
Sbjct: 391 VFKSGKNVLIEFYAPWCGHCKQLAPILDE----VAVSFQSDADVVIAKLDATANDIPTDT 446
Query: 319 YGIRKYPTIKLFRYGEVDKHEYRGARSGPAFVEYI 423
+ ++ YPT+ FR +Y G R+ +E+I
Sbjct: 447 FDVQGYPTL-YFRSASGKLSQYDGGRTKEDIIEFI 480
>sp|P80284|PDI_HORVU Protein disulfide-isomerase precursor (PDI) (Endosperm protein E-1)
Length = 513
Score = 63.9 bits (154), Expect = 2e-10
Identities = 37/135 (27%), Positives = 65/135 (48%), Gaps = 1/135 (0%)
Frame = +1
Query: 106 VIDLTSTNIKTVLDSNDLVLIDFYASWCYFSNILDPIFTEASDLIAAEFXXXXXXXXXXX 285
V+ L + N + + +L++FYA WC L P + +A+ L++ +
Sbjct: 40 VLTLHADNFDDAIGQHPFILVEFYAPWCGHCKSLAPEYEKAAQLLS-KHDPAIVLAKVDA 98
Query: 286 XCERQSDLSKQYGIRKYPTIKLFRYGEVDKHEYRGARSGPAFVEYIRSQLIDPIIYAK-P 462
E+ L+ +Y ++ +PT+K+FR G EY+G R VEY++ Q+ K P
Sbjct: 99 NDEKNKPLAGKYEVQGFPTLKIFRNGGKSIQEYKGpreAEGIVEYLKKQVGPASKEIKAP 158
Query: 463 NEVIVRELGKSYIIG 507
+ E GK +I+G
Sbjct: 159 EDATYLEDGKIHIVG 173
Score = 42.7 bits (99), Expect = 5e-04
Identities = 29/96 (30%), Positives = 43/96 (44%)
Frame = +1
Query: 139 VLDSNDLVLIDFYASWCYFSNILDPIFTEASDLIAAEFXXXXXXXXXXXXCERQSDLSKQ 318
V S VLI+FYA WC L PI EA+ + +E ++D+ +
Sbjct: 395 VFKSGKNVLIEFYAPWCGHCKKLAPILDEAAATLQSE-----EDVVIAKMDATENDVPGE 449
Query: 319 YGIRKYPTIKLFRYGEVDKHEYRGARSGPAFVEYIR 426
+ ++ YPT+ F K Y G R+ V+YIR
Sbjct: 450 FDVQGYPTL-YFVTPSGKKVSYEGGRTADEIVDYIR 484
>sp|P32474|EUG1_YEAST Protein disulfide-isomerase EUG1 precursor (PDI) (Endoplasmic
reticulum protein EUG1)
Length = 517
Score = 63.9 bits (154), Expect = 2e-10
Identities = 42/155 (27%), Positives = 66/155 (42%), Gaps = 1/155 (0%)
Frame = +1
Query: 13 TSALIGTIFENLLMKSFIYILTLVLSVCNCTVIDLTSTNIKTVLDSNDLVLIDFYASWCY 192
T+ I I L SF + ++ LT K+ ++S+ LVL++F+A WC
Sbjct: 4 TTRFISAIVSFCLFASFTLAENSARATPGSDLLVLTEKKFKSFIESHPLVLVEFFAPWCL 63
Query: 193 FSNILDPIFTEASDLIAAEFXXXXXXXXXXXXCERQSDLSKQYGIRKYPTIKLFRYGEV- 369
S IL P EA+ ++ CE S + Q I YPT+K+F+ G +
Sbjct: 64 HSQILRPHLEEAASILKEH-----NVPVVQIDCEANSMVCLQQTINTYPTLKIFKNGRIF 118
Query: 370 DKHEYRGARSGPAFVEYIRSQLIDPIIYAKPNEVI 474
D YRG + +Y+ +IY + I
Sbjct: 119 DGQVYRGVKITDEITQYMIQLYEASVIYLNSEDEI 153
Score = 36.2 bits (82), Expect = 0.047
Identities = 23/84 (27%), Positives = 38/84 (45%)
Frame = +1
Query: 124 TNIKTVLDSNDLVLIDFYASWCYFSNILDPIFTEASDLIAAEFXXXXXXXXXXXXCERQS 303
T+ V D + VL+ +YA+WC S PI+ E ++++A++
Sbjct: 384 THDDIVHDDDKDVLVKYYATWCIHSKRFAPIYEEIANVLASDESVRDKILIAEVDSGAND 443
Query: 304 DLSKQYGIRKYPTIKLFRYGEVDK 375
LS + + YPTI L+ G K
Sbjct: 444 ILS--FPVTGYPTIALYPAGNNSK 465
>sp|P52589|PDI_WHEAT Protein disulfide-isomerase precursor (PDI)
Length = 515
Score = 63.9 bits (154), Expect = 2e-10
Identities = 37/135 (27%), Positives = 66/135 (48%), Gaps = 1/135 (0%)
Frame = +1
Query: 106 VIDLTSTNIKTVLDSNDLVLIDFYASWCYFSNILDPIFTEASDLIAAEFXXXXXXXXXXX 285
V+ L + N + + +L++FYA WC L P + +A+ L++ +
Sbjct: 41 VLTLHADNFDDAIAKHPFILVEFYAPWCGHCKSLAPEYEKAAQLLS-KHDPAIVLAKVDA 99
Query: 286 XCERQSDLSKQYGIRKYPTIKLFRYGEVDKHEYRGARSGPAFVEYIRSQLIDPIIYAK-P 462
E+ L+ +Y ++ +PT+K+FR G + EY+G R VEY++ Q+ K P
Sbjct: 100 NDEKNKPLAGKYEVQGFPTLKIFRSGGKNIQEYKGpreAEGIVEYLKKQVGPASKEIKAP 159
Query: 463 NEVIVRELGKSYIIG 507
+ E GK +I+G
Sbjct: 160 EDATYLEDGKIHIVG 174
Score = 39.7 bits (91), Expect = 0.004
Identities = 28/96 (29%), Positives = 42/96 (43%)
Frame = +1
Query: 139 VLDSNDLVLIDFYASWCYFSNILDPIFTEASDLIAAEFXXXXXXXXXXXXCERQSDLSKQ 318
V S VLI+FYA WC L PI EA+ + +E +D+ +
Sbjct: 396 VFKSAKNVLIEFYAPWCGHCKKLAPILDEAAATLQSE-----EDVVIAKIDATANDVPGE 450
Query: 319 YGIRKYPTIKLFRYGEVDKHEYRGARSGPAFVEYIR 426
+ ++ YPT+ F K Y G R+ V+YI+
Sbjct: 451 FDVQGYPTL-YFVTPSGKKVSYEGGRTADEIVDYIK 485
>sp|Q17967|PDI1_CAEEL Protein disulfide-isomerase 1 precursor (PDI 1) (Prolyl
4-hydroxylase beta 1 subunit)
Length = 485
Score = 62.0 bits (149), Expect = 8e-10
Identities = 42/155 (27%), Positives = 74/155 (47%), Gaps = 3/155 (1%)
Frame = +1
Query: 49 LMKSFIYILTLVLSVCNC---TVIDLTSTNIKTVLDSNDLVLIDFYASWCYFSNILDPIF 219
L SFI++L + V+ LT +N + ++ N+ VL+ FYA WC L P +
Sbjct: 3 LSVSFIFLLVASIGAVVADSENVLVLTESNFEETINGNEFVLVKFYAPWCVHCKSLAPKY 62
Query: 220 TEASDLIAAEFXXXXXXXXXXXXCERQSDLSKQYGIRKYPTIKLFRYGEVDKHEYRGARS 399
EA+DL+ E E Q+ L+ ++ +R YPTI F+ G+ K Y G R+
Sbjct: 63 DEAADLLKEE--GSDIKLAKVDATENQA-LASKFEVRGYPTILYFKSGKPTK--YTGGRA 117
Query: 400 GPAFVEYIRSQLIDPIIYAKPNEVIVRELGKSYII 504
V++++ + + + E + GK+ ++
Sbjct: 118 TAQIVDWVKKKSGPTVTTVESVEQLEELKGKTRVV 152
Score = 34.7 bits (78), Expect = 0.14
Identities = 25/104 (24%), Positives = 43/104 (41%), Gaps = 1/104 (0%)
Frame = +1
Query: 115 LTSTNIKTV-LDSNDLVLIDFYASWCYFSNILDPIFTEASDLIAAEFXXXXXXXXXXXXC 291
L ++N + LD V + FYA WC L P++ E ++ E
Sbjct: 368 LVASNFNEIALDETKTVFVKFYAPWCGHCKQLVPVWDELAE--KYESNPNVVIAKLDATL 425
Query: 292 ERQSDLSKQYGIRKYPTIKLFRYGEVDKHEYRGARSGPAFVEYI 423
+D+ + +PT+KL+ G +Y G R+ F E++
Sbjct: 426 NELADVK----VNSFPTLKLWPAGSSTPVDYDGDRNLEKFEEFV 465
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 51,473,604
Number of Sequences: 369166
Number of extensions: 919493
Number of successful extensions: 2360
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2143
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2234
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3304927425
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)