Planarian EST Database


Dr_sW_009_J18-1

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_009_J18-1
         (476 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8KAH7|RL22_CHLTE  50S ribosomal protein L22                    32   0.58 
sp|Q89A36|PTM3C_BUCBP  PTS system mannitol-specific EIICBA c...    32   0.75 
sp|P49655|IRK10_RAT  ATP-sensitive inward rectifier potassiu...    31   1.3  
sp|Q19981|YC81_CAEEL  Hypothetical protein F33C8.1 in chromo...    31   1.3  
sp|P41695|BUB1_YEAST  Checkpoint serine/threonine-protein ki...    30   2.9  
sp|P78508|IRK10_HUMAN  ATP-sensitive inward rectifier potass...    30   3.7  
sp|Q9JM63|IRK10_MOUSE  ATP-sensitive inward rectifier potass...    30   3.7  
sp|Q9NY15|STAB1_HUMAN  Stabilin-1 precursor (FEEL-1 protein)...    29   6.4  
>sp|Q8KAH7|RL22_CHLTE 50S ribosomal protein L22
          Length = 119

 Score = 32.3 bits (72), Expect = 0.58
 Identities = 24/70 (34%), Positives = 37/70 (52%), Gaps = 3/70 (4%)
 Frame = +3

Query: 165 AILANLRRNLSFITEVLGKTVVANVTESNKTNRINKANIAY*DIFADRGPS-KRVHLSPY 341
           AIL N  ++ S    V  K+ VAN ++ N   R+N   +    IF D GPS KR+  +P 
Sbjct: 34  AILHNSTKSASRNVMVTLKSAVANWSQLNPDERLNDNELFVKAIFVDEGPSLKRLLPAPM 93

Query: 342 --QYKLHKQA 365
              Y++ K++
Sbjct: 94  GRAYRIRKRS 103
>sp|Q89A36|PTM3C_BUCBP PTS system mannitol-specific EIICBA component (EIICBA-Mtl)
           (EII-Mtl) [Includes: Mannitol permease IIC component
           (PTS system mannitol-specific EIIC component);
           Mannitol-specific phosphotransferase enzyme IIB
           component (PTS system mannitol-specific EIIB component);
           Mannitol-specific phosphotransferase enzyme IIA
           component (PTS system mannitol-specific EIIA component)]
          Length = 640

 Score = 32.0 bits (71), Expect = 0.75
 Identities = 31/95 (32%), Positives = 43/95 (45%), Gaps = 2/95 (2%)
 Frame = +3

Query: 87  KNN*LI-SKFQKTSSCIGSVLQLGLCLAILANLRRNLSFITEV-LGKTVVANVTESNKTN 260
           K+N LI S FQK  +CI S L    C+       RN+ F  +  +G + VA     NK +
Sbjct: 352 KDNFLINSSFQKNRTCIKSSLDSHKCI-------RNIIFACDAGMGSSAVAAGILRNKIH 404

Query: 261 RINKANIAY*DIFADRGPSKRVHLSPYQYKLHKQA 365
            +N  NI   +   D  P+  V L    Y L  +A
Sbjct: 405 DLNIFNITVSNAAIDSIPNFGVDLIITHYSLTDRA 439
>sp|P49655|IRK10_RAT ATP-sensitive inward rectifier potassium channel 10 (Potassium
           channel, inwardly rectifying subfamily J member 10)
           (ATP-sensitive inward rectifier potassium channel KAB-2)
           (Kir4.1) (BIR10) (Brain-specific inwardly rectifying
           K(+) channel 1) (BIRK1)
          Length = 379

 Score = 31.2 bits (69), Expect = 1.3
 Identities = 15/45 (33%), Positives = 28/45 (62%), Gaps = 1/45 (2%)
 Frame = +3

Query: 156 LCLAI-LANLRRNLSFITEVLGKTVVANVTESNKTNRINKANIAY 287
           LCL I +AN+R++L    +V GK +  + T+  +  R+N+ N+ +
Sbjct: 194 LCLMIRVANMRKSLLIGCQVTGKLLQTHQTKEGENIRLNQVNVTF 238
>sp|Q19981|YC81_CAEEL Hypothetical protein F33C8.1 in chromosome X precursor
          Length = 1271

 Score = 31.2 bits (69), Expect = 1.3
 Identities = 11/32 (34%), Positives = 18/32 (56%)
 Frame = -2

Query: 421 CHHGICIWTEATPFIPSKKACLCNLYWYGDRC 326
           C++G+C+           KAC+C+  WYG +C
Sbjct: 70  CYNGVCL----------NKACVCSKGWYGSQC 91
>sp|P41695|BUB1_YEAST Checkpoint serine/threonine-protein kinase BUB1
          Length = 1021

 Score = 30.0 bits (66), Expect = 2.9
 Identities = 11/35 (31%), Positives = 26/35 (74%)
 Frame = -3

Query: 168 LLDKGLIVKLTLYMKKFSEIWKSINYFLEITLVVE 64
           + +KG+  KL+L+ ++FS++ ++  +FLE  +++E
Sbjct: 133 MFNKGIGTKLSLFYEEFSKLLENAQFFLEAKVLLE 167
>sp|P78508|IRK10_HUMAN ATP-sensitive inward rectifier potassium channel 10 (Potassium
           channel, inwardly rectifying subfamily J member 10)
           (Inward rectifier K(+) channel Kir1.2) (ATP-dependent
           inwardly rectifying potassium channel Kir4.1)
          Length = 379

 Score = 29.6 bits (65), Expect = 3.7
 Identities = 14/44 (31%), Positives = 27/44 (61%), Gaps = 1/44 (2%)
 Frame = +3

Query: 159 CLAI-LANLRRNLSFITEVLGKTVVANVTESNKTNRINKANIAY 287
           CL I +AN+R++L    +V GK +  + T+  +  R+N+ N+ +
Sbjct: 195 CLMIRVANMRKSLLIGCQVTGKLLQTHQTKEGENIRLNQVNVTF 238
>sp|Q9JM63|IRK10_MOUSE ATP-sensitive inward rectifier potassium channel 10 (Potassium
           channel, inwardly rectifying subfamily J member 10)
           (Inward rectifier K(+) channel Kir4.1)
          Length = 379

 Score = 29.6 bits (65), Expect = 3.7
 Identities = 14/44 (31%), Positives = 27/44 (61%), Gaps = 1/44 (2%)
 Frame = +3

Query: 159 CLAI-LANLRRNLSFITEVLGKTVVANVTESNKTNRINKANIAY 287
           CL I +AN+R++L    +V GK +  + T+  +  R+N+ N+ +
Sbjct: 195 CLMIRVANMRKSLLIGCQVTGKLLQTHQTKEGENIRLNQVNVTF 238
>sp|Q9NY15|STAB1_HUMAN Stabilin-1 precursor (FEEL-1 protein) (MS-1 antigen)
          Length = 2570

 Score = 28.9 bits (63), Expect = 6.4
 Identities = 19/52 (36%), Positives = 24/52 (46%), Gaps = 4/52 (7%)
 Frame = -2

Query: 439  DLGEILCHHGIC-IWTEATPFIPSKKACLCNLYWYGD---RCTLFDGPLSAN 296
            ++   L HHG C I  E  P  P + +C C   + GD    C L D P S N
Sbjct: 1497 EINSCLIHHGGCHIHAECIPTGPQQVSCSCREGYSGDGIRTCELLD-PCSKN 1547
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 51,581,030
Number of Sequences: 369166
Number of extensions: 934688
Number of successful extensions: 2312
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 2248
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2312
length of database: 68,354,980
effective HSP length: 101
effective length of database: 49,696,745
effective search space used: 2832714465
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)