Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_009_J15
(676 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8CIF6|SIDT2_MOUSE SID1 transmembrane family member 2 pr... 77 3e-14
sp|Q8NBJ9|SIDT2_HUMAN SID1 transmembrane family member 2 pr... 77 3e-14
sp|Q6AXF6|SIDT1_MOUSE SID1 transmembrane family member 1 pr... 71 3e-12
sp|Q9NXL6|SIDT1_HUMAN SID1 transmembrane family member 1 pr... 71 3e-12
sp|Q6Q3F5|SIDT1_RAT SID1 transmembrane family member 1 prec... 71 3e-12
sp|Q9GZC8|SID1_CAEEL Systemic RNA interference defective pr... 57 6e-08
sp|P43548|AGP3_YEAST General amino acid permease AGP3 33 0.68
sp|P14151|LYAM1_HUMAN L-selectin precursor (Lymph node homi... 32 1.2
sp|Q95237|LYAM1_PANTR L-selectin precursor (Lymph node homi... 32 1.2
sp|Q95235|LYAM1_PONPY L-selectin precursor (Lymph node homi... 32 1.2
>sp|Q8CIF6|SIDT2_MOUSE SID1 transmembrane family member 2 precursor
Length = 832
Score = 77.4 bits (189), Expect = 3e-14
Identities = 38/112 (33%), Positives = 64/112 (57%)
Frame = +2
Query: 155 FFILHNLFIKNNCRVKFIEKFCLIWKKYKFISINLIASVIIWGLAYSAFRNAPTDWNSSA 334
F+I+ L ++ R+K I C++ + ++WG A F + W +
Sbjct: 732 FYIIMKL--RSGERIKLIPLLCIV------------CTSVVWGFALFFFFQGLSTWQKTP 777
Query: 335 SESRESNQDCIWWNYYDIHDIWHILSSIALFLSFMWIFMIDEEIEKNQREPI 490
+ESRE N+DCI +++D HDIWH LSSIA+F SF+ + +D++++ QR+ I
Sbjct: 778 AESREHNRDCILLDFFDDHDIWHFLSSIAMFGSFLVLLTLDDDLDTVQRDKI 829
>sp|Q8NBJ9|SIDT2_HUMAN SID1 transmembrane family member 2 precursor
Length = 832
Score = 77.4 bits (189), Expect = 3e-14
Identities = 38/112 (33%), Positives = 64/112 (57%)
Frame = +2
Query: 155 FFILHNLFIKNNCRVKFIEKFCLIWKKYKFISINLIASVIIWGLAYSAFRNAPTDWNSSA 334
F+I+ L ++ R+K I C++ + ++WG A F + W +
Sbjct: 732 FYIIMKL--RSGERIKLIPLLCIV------------CTSVVWGFALFFFFQGLSTWQKTP 777
Query: 335 SESRESNQDCIWWNYYDIHDIWHILSSIALFLSFMWIFMIDEEIEKNQREPI 490
+ESRE N+DCI +++D HDIWH LSSIA+F SF+ + +D++++ QR+ I
Sbjct: 778 AESREHNRDCILLDFFDDHDIWHFLSSIAMFGSFLVLLTLDDDLDTVQRDKI 829
>sp|Q6AXF6|SIDT1_MOUSE SID1 transmembrane family member 1 precursor
Length = 827
Score = 70.9 bits (172), Expect = 3e-12
Identities = 39/152 (25%), Positives = 78/152 (51%), Gaps = 2/152 (1%)
Frame = +2
Query: 41 LVAIVVNFVVLLLEYYFTLSENIEFWKYEILFFGFNHIFFILHNLFIK--NNCRVKFIEK 214
++ IV N V ++ + +F Y + F N + ++ + +K ++ +V +
Sbjct: 685 VLLIVGNLVNWSFAFFGLIYRPRDFASYMLGIFICNLLLYLAFYIIMKLRSSEKVLPLPV 744
Query: 215 FCLIWKKYKFISINLIASVIIWGLAYSAFRNAPTDWNSSASESRESNQDCIWWNYYDIHD 394
FC+ A+ ++W A F + W + +ESRE N++C+ +++D HD
Sbjct: 745 FCIA------------ATAVVWAAALYFFFQNLSSWEGTPAESREKNRECVLLDFFDDHD 792
Query: 395 IWHILSSIALFLSFMWIFMIDEEIEKNQREPI 490
IWH LS+ ALF SF+ + +D++++ +R+ I
Sbjct: 793 IWHFLSATALFFSFLVLLTLDDDLDVVRRDQI 824
>sp|Q9NXL6|SIDT1_HUMAN SID1 transmembrane family member 1 precursor
Length = 827
Score = 70.9 bits (172), Expect = 3e-12
Identities = 28/78 (35%), Positives = 51/78 (65%)
Frame = +2
Query: 257 LIASVIIWGLAYSAFRNAPTDWNSSASESRESNQDCIWWNYYDIHDIWHILSSIALFLSF 436
++A+ ++W A F + W + +ESRE N++CI +++D HDIWH LS+ ALF SF
Sbjct: 747 IVATAVMWAAALYFFFQNLSSWEGTPAESREKNRECILLDFFDDHDIWHFLSATALFFSF 806
Query: 437 MWIFMIDEEIEKNQREPI 490
+ + +D++++ +R+ I
Sbjct: 807 LVLLTLDDDLDVVRRDQI 824
>sp|Q6Q3F5|SIDT1_RAT SID1 transmembrane family member 1 precursor
Length = 831
Score = 70.9 bits (172), Expect = 3e-12
Identities = 27/78 (34%), Positives = 50/78 (64%)
Frame = +2
Query: 257 LIASVIIWGLAYSAFRNAPTDWNSSASESRESNQDCIWWNYYDIHDIWHILSSIALFLSF 436
++A+ ++W A F + W + +ESRE N++C+ ++D HDIWH LS+ ALF SF
Sbjct: 751 IVATAVVWAAALYFFFQNLSSWEGTPAESREKNRECVLLGFFDDHDIWHFLSATALFFSF 810
Query: 437 MWIFMIDEEIEKNQREPI 490
+ + +D++++ +R+ I
Sbjct: 811 LVLLTLDDDLDVVRRDQI 828
>sp|Q9GZC8|SID1_CAEEL Systemic RNA interference defective protein 1 precursor (Systemic
RNAi enabling protein)
Length = 776
Score = 56.6 bits (135), Expect = 6e-08
Identities = 22/75 (29%), Positives = 43/75 (57%)
Frame = +2
Query: 266 SVIIWGLAYSAFRNAPTDWNSSASESRESNQDCIWWNYYDIHDIWHILSSIALFLSFMWI 445
S++ W +A F TDW SA+ SR N+ C+ ++ HD+WHI ++A +F+++
Sbjct: 699 SLLAWAVAGFFFFQDDTDWTRSAAASRALNKPCLLLGFFGSHDLWHIFGALAGLFTFIFV 758
Query: 446 FMIDEEIEKNQREPI 490
+D+++ ++ I
Sbjct: 759 SFVDDDLINTRKTSI 773
>sp|P43548|AGP3_YEAST General amino acid permease AGP3
Length = 558
Score = 33.1 bits (74), Expect = 0.68
Identities = 29/105 (27%), Positives = 47/105 (44%), Gaps = 6/105 (5%)
Frame = +2
Query: 47 AIVVNFVVLLLEYYFTLSENIEFWKYEILFFGFNHIFFILHNLFIKNNCRVKFIEKFCLI 226
A VV F +L Y TLS ++FW ++ +G+ IF+ +F + ++ L
Sbjct: 137 AYVVVFFAVLANEYNTLSSILQFWGPQVPLYGYILIFWFAFEIFQLVGVGLFGETEYWLA 196
Query: 227 WKKYKFISINLIASVIIWGLAYSA--FRNAPT----DWNSSASES 343
W K + L+A I+ + Y + RN P WNS + S
Sbjct: 197 WLKI----VGLVA-YYIFSIVYISGDIRNRPAFGFHYWNSPGALS 236
>sp|P14151|LYAM1_HUMAN L-selectin precursor (Lymph node homing receptor) (Leukocyte
adhesion molecule 1) (LAM-1) (Leukocyte surface antigen
Leu-8) (TQ1) (gp90-MEL) (Leukocyte-endothelial cell
adhesion molecule 1) (LECAM1) (CD62L antigen)
Length = 372
Score = 32.3 bits (72), Expect = 1.2
Identities = 12/63 (19%), Positives = 28/63 (44%)
Frame = +2
Query: 179 IKNNCRVKFIEKFCLIWKKYKFISINLIASVIIWGLAYSAFRNAPTDWNSSASESRESNQ 358
I+N ++++EK + Y +I I I + W + +W ++++ +
Sbjct: 67 IQNKAEIEYLEKTLPFSRSYYWIGIRKIGGIWTWVGTNKSLTEEAENWGDGEPNNKKNKE 126
Query: 359 DCI 367
DC+
Sbjct: 127 DCV 129
>sp|Q95237|LYAM1_PANTR L-selectin precursor (Lymph node homing receptor) (Leukocyte
adhesion molecule 1) (LAM-1) (Leukocyte-endothelial cell
adhesion molecule 1) (LECAM1) (CD62L antigen)
Length = 372
Score = 32.3 bits (72), Expect = 1.2
Identities = 12/63 (19%), Positives = 28/63 (44%)
Frame = +2
Query: 179 IKNNCRVKFIEKFCLIWKKYKFISINLIASVIIWGLAYSAFRNAPTDWNSSASESRESNQ 358
I+N ++++EK + Y +I I I + W + +W ++++ +
Sbjct: 67 IQNKAEIEYLEKTLPFSRSYYWIGIRKIGGIWTWVGTNKSLTEEAENWGDGEPNNKKNKE 126
Query: 359 DCI 367
DC+
Sbjct: 127 DCV 129
>sp|Q95235|LYAM1_PONPY L-selectin precursor (Lymph node homing receptor) (Leukocyte
adhesion molecule 1) (LAM-1) (Leukocyte-endothelial cell
adhesion molecule 1) (LECAM1) (CD62L antigen)
Length = 372
Score = 32.3 bits (72), Expect = 1.2
Identities = 12/63 (19%), Positives = 28/63 (44%)
Frame = +2
Query: 179 IKNNCRVKFIEKFCLIWKKYKFISINLIASVIIWGLAYSAFRNAPTDWNSSASESRESNQ 358
I+N ++++EK + Y +I I I + W + +W ++++ +
Sbjct: 67 IQNKAEIEYLEKTLPFSRSYYWIGIRKIGGIWTWVGTNKSLTEEAENWGDGEPNNKKNKE 126
Query: 359 DCI 367
DC+
Sbjct: 127 DCV 129
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 67,913,328
Number of Sequences: 369166
Number of extensions: 1277702
Number of successful extensions: 3303
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3204
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3301
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5684835195
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)