Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_009_J13
(803 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9NLA1|FLR4_CAEEL Serine/threonine-protein kinase flr-4 ... 64 4e-10
sp|Q40531|NTF6_TOBAC Mitogen-activated protein kinase homol... 62 1e-09
sp|P28482|MK01_HUMAN Mitogen-activated protein kinase 1 (Ex... 62 2e-09
sp|P63086|MK01_RAT Mitogen-activated protein kinase 1 (Extr... 62 2e-09
sp|P46196|MK01_BOVIN Mitogen-activated protein kinase 1 (Ex... 62 2e-09
sp|P26696|MK01_XENLA Mitogen-activated protein kinase 1 (My... 62 2e-09
sp|Q9NYV4|CD2L7_HUMAN Cell division cycle 2-related protein... 61 4e-09
sp|Q39027|MPK7_ARATH Mitogen-activated protein kinase homol... 60 5e-09
sp|P50528|PLO1_SCHPO Serine/threonine-protein kinase plo1 60 9e-09
sp|P23293|SGV1_YEAST Serine/threonine-protein kinase SGV1 59 1e-08
>sp|Q9NLA1|FLR4_CAEEL Serine/threonine-protein kinase flr-4 (Fluoride resistant protein
4)
Length = 570
Score = 64.3 bits (155), Expect = 4e-10
Identities = 57/201 (28%), Positives = 93/201 (46%), Gaps = 9/201 (4%)
Frame = +1
Query: 199 HNFENLQLFSTSAFLE--DSKQLLCYPMRN---DCEIFLLDMEKKRSVTISEKIKDGKYA 363
+N ++L +S L+ D + L PMR D ++ D+ K R T+ + +
Sbjct: 5 YNRNAVELKLSSQLLQWLDQRLPLGDPMRIPSIDSYKYIQDLGKGRFGTVCKFSNGNTFE 64
Query: 364 KIHYGDLDTPPETCVIFQYDISRTNVKN-FKMLKYEMVTRFKLPYDTSRFVQLLSMYEEE 540
+ DL + + S T V N YE K+ D +R V L +Y +
Sbjct: 65 TVKKVDLT------IFNHWTQSETKVSNRLDTFLYEFRHLHKVTNDNNRIVNFLGIYADS 118
Query: 541 NSVFIVMERMA-SSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLK--ILHRSLNCG 711
N ++I+ E + S++ LL +T L ED KY E ++ L Y+H L ++HR +
Sbjct: 119 NQMYIMSEYLPRGSVKDLLVKET--LGEDTAIKYLMETVEALDYLHNLSPPVIHRDIKAA 176
Query: 712 SVLIDCNDNAKLCNFHLSKFL 774
++LI ND+ KL NF L + L
Sbjct: 177 NLLITSNDSIKLANFGLVRDL 197
>sp|Q40531|NTF6_TOBAC Mitogen-activated protein kinase homolog NTF6 (P43)
Length = 371
Score = 62.4 bits (150), Expect = 1e-09
Identities = 29/79 (36%), Positives = 51/79 (64%)
Frame = +1
Query: 532 EEENSVFIVMERMASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKILHRSLNCG 711
EE N V+IV E M + L +++ +Q LT+D + + Y++L GL Y+H +LHR L
Sbjct: 110 EEFNDVYIVYELMDTDLHQIIR-SSQALTDDHCQYFLYQLLRGLKYVHSANVLHRDLKPS 168
Query: 712 SVLIDCNDNAKLCNFHLSK 768
++L++ N + K+C+F L++
Sbjct: 169 NLLLNANCDLKICDFGLAR 187
>sp|P28482|MK01_HUMAN Mitogen-activated protein kinase 1 (Extracellular signal-regulated
kinase 2) (ERK-2) (Mitogen-activated protein kinase 2)
(MAP kinase 2) (MAPK 2) (p42-MAPK) (ERT1)
Length = 360
Score = 62.0 bits (149), Expect = 2e-09
Identities = 31/79 (39%), Positives = 49/79 (62%)
Frame = +1
Query: 532 EEENSVFIVMERMASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKILHRSLNCG 711
E+ V+IV + M + L LLK TQHL+ D I + Y+IL GL Y+H +LHR L
Sbjct: 96 EQMKDVYIVQDLMETDLYKLLK--TQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPS 153
Query: 712 SVLIDCNDNAKLCNFHLSK 768
++L++ + K+C+F L++
Sbjct: 154 NLLLNTTCDLKICDFGLAR 172
>sp|P63086|MK01_RAT Mitogen-activated protein kinase 1 (Extracellular signal-regulated
kinase 2) (ERK-2) (Mitogen-activated protein kinase 2)
(MAP kinase 2) (MAPK 2) (p42-MAPK) (ERT1)
sp|P63085|MK01_MOUSE Mitogen-activated protein kinase 1 (Extracellular signal-regulated
kinase 2) (ERK-2) (Mitogen-activated protein kinase 2)
(MAP kinase 2) (MAPK 2) (p42-MAPK) (ERT1)
Length = 358
Score = 62.0 bits (149), Expect = 2e-09
Identities = 31/79 (39%), Positives = 49/79 (62%)
Frame = +1
Query: 532 EEENSVFIVMERMASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKILHRSLNCG 711
E+ V+IV + M + L LLK TQHL+ D I + Y+IL GL Y+H +LHR L
Sbjct: 94 EQMKDVYIVQDLMETDLYKLLK--TQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPS 151
Query: 712 SVLIDCNDNAKLCNFHLSK 768
++L++ + K+C+F L++
Sbjct: 152 NLLLNTTCDLKICDFGLAR 170
>sp|P46196|MK01_BOVIN Mitogen-activated protein kinase 1 (Extracellular signal-regulated
kinase 2) (ERK-2) (Mitogen-activated protein kinase 2)
(MAP kinase 2) (MAPK 2) (p42-MAPK) (ERT1)
Length = 360
Score = 62.0 bits (149), Expect = 2e-09
Identities = 31/79 (39%), Positives = 49/79 (62%)
Frame = +1
Query: 532 EEENSVFIVMERMASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKILHRSLNCG 711
E+ V+IV + M + L LLK TQHL+ D I + Y+IL GL Y+H +LHR L
Sbjct: 96 EQMKDVYIVQDLMETDLYKLLK--TQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPS 153
Query: 712 SVLIDCNDNAKLCNFHLSK 768
++L++ + K+C+F L++
Sbjct: 154 NLLLNTTCDLKICDFGLAR 172
>sp|P26696|MK01_XENLA Mitogen-activated protein kinase 1 (Myelin xP42 protein kinase)
(Myelin basic protein kinase) (MBP kinase) (M phase MAP
kinase)
Length = 361
Score = 62.0 bits (149), Expect = 2e-09
Identities = 31/79 (39%), Positives = 49/79 (62%)
Frame = +1
Query: 532 EEENSVFIVMERMASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKILHRSLNCG 711
E+ V+IV + M + L LLK TQHL+ D I + Y+IL GL Y+H +LHR L
Sbjct: 99 EQMKDVYIVQDLMETDLYKLLK--TQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPS 156
Query: 712 SVLIDCNDNAKLCNFHLSK 768
++L++ + K+C+F L++
Sbjct: 157 NLLLNTTCDLKICDFGLAR 175
>sp|Q9NYV4|CD2L7_HUMAN Cell division cycle 2-related protein kinase 7 (CDC2-related protein
kinase 7) (CrkRS)
Length = 1490
Score = 60.8 bits (146), Expect = 4e-09
Identities = 26/83 (31%), Positives = 48/83 (57%)
Frame = +1
Query: 520 LSMYEEENSVFIVMERMASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKILHRS 699
L +++ + ++V E M L LL+ H +ED IK + ++++GL Y HK LHR
Sbjct: 800 LDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFSEDHIKSFMKQLMEGLEYCHKKNFLHRD 859
Query: 700 LNCGSVLIDCNDNAKLCNFHLSK 768
+ C ++L++ + KL +F L++
Sbjct: 860 IKCSNILLNNSGQIKLADFGLAR 882
>sp|Q39027|MPK7_ARATH Mitogen-activated protein kinase homolog 7 (MAP kinase 7) (AtMPK7)
Length = 368
Score = 60.5 bits (145), Expect = 5e-09
Identities = 35/113 (30%), Positives = 63/113 (55%), Gaps = 3/113 (2%)
Frame = +1
Query: 439 VKNFKMLKYEMVTRFK---LPYDTSRFVQLLSMYEEENSVFIVMERMASSLRSLLKPDTQ 609
+K + +++E V K LP + S F V++V E M + L ++K +Q
Sbjct: 80 LKLLRHVRHENVIALKDVMLPANRSSF----------KDVYLVYELMDTDLHQIIK-SSQ 128
Query: 610 HLTEDKIKKYSYEILDGLSYMHKLKILHRSLNCGSVLIDCNDNAKLCNFHLSK 768
L++D K + +++L GL Y+H ILHR L G++L++ N + K+C+F L++
Sbjct: 129 SLSDDHCKYFLFQLLRGLKYLHSANILHRDLKPGNLLVNANCDLKICDFGLAR 181
>sp|P50528|PLO1_SCHPO Serine/threonine-protein kinase plo1
Length = 683
Score = 59.7 bits (143), Expect = 9e-09
Identities = 35/122 (28%), Positives = 69/122 (56%), Gaps = 3/122 (2%)
Frame = +1
Query: 424 ISRTNVKNFKMLKYEMVTRFKLPYDTSR--FVQLLSMYEEENSVFIVMERMA-SSLRSLL 594
I++ +++N K K ++ K+ S V + +E+ ++++++E SL LL
Sbjct: 71 IAKRSLQNDKT-KLKLFGEIKVHQSMSHPNIVGFIDCFEDSTNIYLILELCEHKSLMELL 129
Query: 595 KPDTQHLTEDKIKKYSYEILDGLSYMHKLKILHRSLNCGSVLIDCNDNAKLCNFHLSKFL 774
+ Q LTE +++ +IL L YMHK +++HR L G++++D ++N K+ +F L+ L
Sbjct: 130 RKRKQ-LTEPEVRYLMMQILGALKYMHKKRVIHRDLKLGNIMLDESNNVKIGDFGLAALL 188
Query: 775 FD 780
D
Sbjct: 189 MD 190
>sp|P23293|SGV1_YEAST Serine/threonine-protein kinase SGV1
Length = 657
Score = 59.3 bits (142), Expect = 1e-08
Identities = 50/217 (23%), Positives = 100/217 (46%), Gaps = 18/217 (8%)
Frame = +1
Query: 187 RTKFHNFENLQLFSTSAFLEDSKQLLCYPMRNDCEIFLLDMEKKRSVTI-------SEKI 345
+T+F+ ++ ++ ST A D+K L Y ++ EK T+ EK+
Sbjct: 12 KTEFNKYKIGKVKSTPAIQRDAKTNLTY-----IKLRKRSSEKVYGCTVFQNHYREDEKL 66
Query: 346 KDGKYAKIHYG-DLDTPPETC-----VIFQYDI----SRTNVKNFKMLKYEMVTRF-KLP 492
G + +++ G L+T + V + D+ ++ + K L ++ + + ++
Sbjct: 67 GQGTFGEVYKGIHLETQRQVAMKKIIVSVEKDLFPITAQREITILKRLNHKNIIKLIEMV 126
Query: 493 YDTSRFVQLLSMYEEENSVFIVMERMASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYM 672
YD S + + S ++++ M + L +L +L IK +IL+GL+Y+
Sbjct: 127 YDHSPDITNAASSNLHKSFYMILPYMVADLSGVLHNPRINLEMCDIKNMMLQILEGLNYI 186
Query: 673 HKLKILHRSLNCGSVLIDCNDNAKLCNFHLSKFLFDC 783
H K +HR + ++LID N KL +F L++ + C
Sbjct: 187 HCAKFMHRDIKTANILIDHNGVLKLADFGLARLYYGC 223
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 93,973,765
Number of Sequences: 369166
Number of extensions: 1914755
Number of successful extensions: 6525
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6259
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6489
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7618580670
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)