Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_009_I13
(867 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q68FX0|IDH3B_RAT Isocitrate dehydrogenase [NAD] subunit ... 259 9e-69
sp|O43837|IDH3B_HUMAN Isocitrate dehydrogenase [NAD] subuni... 258 1e-68
sp|O77784|IDH3B_BOVIN Isocitrate dehydrogenase [NAD] subuni... 258 1e-68
sp|Q28479|IDH3B_MACFA Isocitrate dehydrogenase [NAD] subuni... 254 3e-67
sp|Q5RBT4|IDH3B_PONPY Isocitrate dehydrogenase [NAD] subuni... 253 5e-67
sp|Q93353|IDH3B_CAEEL Probable isocitrate dehydrogenase [NA... 251 1e-66
sp|O94229|IDH1_KLULA Isocitrate dehydrogenase [NAD] subunit... 224 3e-58
sp|P28834|IDH1_YEAST Isocitrate dehydrogenase [NAD] subunit... 223 4e-58
sp|P41565|IDH3G_RAT Isocitrate dehydrogenase [NAD] subunit ... 222 1e-57
sp|P41564|IDH3G_MACFA Isocitrate dehydrogenase [NAD] subuni... 221 2e-57
>sp|Q68FX0|IDH3B_RAT Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
precursor (Isocitric dehydrogenase) (NAD(+)-specific
ICDH)
Length = 385
Score = 259 bits (661), Expect = 9e-69
Identities = 126/240 (52%), Positives = 165/240 (68%)
Frame = +1
Query: 1 NIDFVVIREQTEGEYSALEHESVPGVVESLKIITKSKSERIAKFAFDFAKKHGRKKVTAV 180
N+D V+IREQTEGEYS+LEHES GV+E LKI+T++KS+RIAKFAFD+A K GR KVTAV
Sbjct: 157 NLDLVIIREQTEGEYSSLEHESARGVIECLKIVTRTKSQRIAKFAFDYATKKGRSKVTAV 216
Query: 181 HKANIM*LVMXXXXXXXXXXXXKLGDGLFLESCRNVSKLYPDIKLDDIIVDNCCMQLVSN 360
HKANIM KLGDGLFL+ C V++LYP IK + +I+DNCCMQLV N
Sbjct: 217 HKANIM----------------KLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQN 260
Query: 361 PQQFDVLVTPNLYGAVVDNXXXXXXXXXXXXXXXSFGYDVAIFEPGSRHPFAMAAGKNIA 540
P QFDVLV PNLYG ++DN S+ + A+FE G+RHPFA A G+NIA
Sbjct: 261 PYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAEYAVFETGARHPFAQAVGRNIA 320
Query: 541 NPIGLFLSAVGMLHHINMHSQAIQLESAILNVLKDGEVRTEDIGGTNSTTEIVEAIISQV 720
NP + LSA ML H+N+ + + A+ V+K G+VRT D+GG ++TT+ ++++I +
Sbjct: 321 NPTAMLLSASNMLRHLNLEYHSSMIADAVKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHL 380
>sp|O43837|IDH3B_HUMAN Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
precursor (Isocitric dehydrogenase) (NAD(+)-specific
ICDH)
Length = 385
Score = 258 bits (660), Expect = 1e-68
Identities = 126/241 (52%), Positives = 166/241 (68%)
Frame = +1
Query: 1 NIDFVVIREQTEGEYSALEHESVPGVVESLKIITKSKSERIAKFAFDFAKKHGRKKVTAV 180
N+D V+IREQTEGEYS+LEHES GV+E LKI+T++KS+RIAKFAFD+A K GR KVTAV
Sbjct: 157 NLDLVIIREQTEGEYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKGRGKVTAV 216
Query: 181 HKANIM*LVMXXXXXXXXXXXXKLGDGLFLESCRNVSKLYPDIKLDDIIVDNCCMQLVSN 360
HKANIM KLGDGLFL+ C V++LYP IK + +I+DNCCMQLV N
Sbjct: 217 HKANIM----------------KLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQN 260
Query: 361 PQQFDVLVTPNLYGAVVDNXXXXXXXXXXXXXXXSFGYDVAIFEPGSRHPFAMAAGKNIA 540
P QFDVLV PNLYG ++DN S+ + A+FE G+RHPFA A G+NIA
Sbjct: 261 PYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAEYAVFETGARHPFAQAVGRNIA 320
Query: 541 NPIGLFLSAVGMLHHINMHSQAIQLESAILNVLKDGEVRTEDIGGTNSTTEIVEAIISQV 720
NP + LSA ML H+N+ + + A+ V+K G+VRT D+GG ++TT+ ++++I +
Sbjct: 321 NPTAMLLSASNMLRHLNLEYHSSMIADAVKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHL 380
Query: 721 K 723
+
Sbjct: 381 Q 381
>sp|O77784|IDH3B_BOVIN Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
precursor (Isocitric dehydrogenase) (NAD(+)-specific
ICDH) (NAD(+)-isocitrate dehydrogenase subunit 1) (IDH1)
Length = 385
Score = 258 bits (659), Expect = 1e-68
Identities = 126/240 (52%), Positives = 165/240 (68%)
Frame = +1
Query: 1 NIDFVVIREQTEGEYSALEHESVPGVVESLKIITKSKSERIAKFAFDFAKKHGRKKVTAV 180
N+D V+IREQTEGEYS+LEHES GV+E LKI+T++KS+RIAKFAFD+A K GR KVTAV
Sbjct: 157 NLDLVIIREQTEGEYSSLEHESARGVIECLKIVTRTKSQRIAKFAFDYATKKGRGKVTAV 216
Query: 181 HKANIM*LVMXXXXXXXXXXXXKLGDGLFLESCRNVSKLYPDIKLDDIIVDNCCMQLVSN 360
HKANIM KLGDGLFL+ C V++LYP IK + +I+DNCCMQLV N
Sbjct: 217 HKANIM----------------KLGDGLFLQCCEEVAELYPKIKFEKMIIDNCCMQLVQN 260
Query: 361 PQQFDVLVTPNLYGAVVDNXXXXXXXXXXXXXXXSFGYDVAIFEPGSRHPFAMAAGKNIA 540
P QFDVLV PNLYG ++DN S+ + A+FE G+RHPFA A G+NIA
Sbjct: 261 PYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAEYAVFETGARHPFAQAVGRNIA 320
Query: 541 NPIGLFLSAVGMLHHINMHSQAIQLESAILNVLKDGEVRTEDIGGTNSTTEIVEAIISQV 720
NP + LSA ML H+N+ + + A+ V+K G+VRT D+GG ++TT+ ++++I +
Sbjct: 321 NPTAMLLSASNMLRHLNLEHHSNMIAEAVKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHL 380
>sp|Q28479|IDH3B_MACFA Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
precursor (Isocitric dehydrogenase) (NAD(+)-specific
ICDH)
Length = 381
Score = 254 bits (648), Expect = 3e-67
Identities = 126/238 (52%), Positives = 161/238 (67%)
Frame = +1
Query: 1 NIDFVVIREQTEGEYSALEHESVPGVVESLKIITKSKSERIAKFAFDFAKKHGRKKVTAV 180
N+D V+IREQTEGEYS+LEHES GV+E LKI+T++KS+RIAKFAFD+A K GR KVTAV
Sbjct: 155 NLDLVIIREQTEGEYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKGRSKVTAV 214
Query: 181 HKANIM*LVMXXXXXXXXXXXXKLGDGLFLESCRNVSKLYPDIKLDDIIVDNCCMQLVSN 360
HKANIM KLGDGLFL+ C V++LYP IK + +I+DNCCMQLV N
Sbjct: 215 HKANIM----------------KLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQN 258
Query: 361 PQQFDVLVTPNLYGAVVDNXXXXXXXXXXXXXXXSFGYDVAIFEPGSRHPFAMAAGKNIA 540
P QFDVLV PNLYG ++DN S+ + A+FE G+RHPFA A G+NIA
Sbjct: 259 PYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAEYAVFETGARHPFAQAVGRNIA 318
Query: 541 NPIGLFLSAVGMLHHINMHSQAIQLESAILNVLKDGEVRTEDIGGTNSTTEIVEAIIS 714
NP + LSA ML H+N+ + + A+ V+K G+VRT D+GG + + EA+I+
Sbjct: 319 NPTAMLLSASNMLRHLNLEYHSNMIADAVKKVIKVGKVRTSDMGGYATCHDFTEAVIA 376
>sp|Q5RBT4|IDH3B_PONPY Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
precursor (Isocitric dehydrogenase) (NAD(+)-specific
ICDH)
Length = 385
Score = 253 bits (646), Expect = 5e-67
Identities = 124/240 (51%), Positives = 163/240 (67%)
Frame = +1
Query: 1 NIDFVVIREQTEGEYSALEHESVPGVVESLKIITKSKSERIAKFAFDFAKKHGRKKVTAV 180
N+D V+IREQTEGE S+LEHES GV+E LKI+T++KS+RIAKFAFD+A K GR KV AV
Sbjct: 157 NLDLVIIREQTEGECSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKGRSKVIAV 216
Query: 181 HKANIM*LVMXXXXXXXXXXXXKLGDGLFLESCRNVSKLYPDIKLDDIIVDNCCMQLVSN 360
HKANIM KLGDGLFL+ C V++LYP IK + +I+DNCCMQLV N
Sbjct: 217 HKANIM----------------KLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQN 260
Query: 361 PQQFDVLVTPNLYGAVVDNXXXXXXXXXXXXXXXSFGYDVAIFEPGSRHPFAMAAGKNIA 540
P QFDVLV PNLYG ++DN S+ + A+FE G+RHPFA A G+NIA
Sbjct: 261 PYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAEYAVFETGARHPFAQAVGRNIA 320
Query: 541 NPIGLFLSAVGMLHHINMHSQAIQLESAILNVLKDGEVRTEDIGGTNSTTEIVEAIISQV 720
NP + LSA ML H+N+ + + A+ V+K G+VRT D+GG ++TT+ ++++I +
Sbjct: 321 NPTAMLLSASNMLRHLNLEYHSSMIADAVKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHL 380
>sp|Q93353|IDH3B_CAEEL Probable isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
precursor (Isocitric dehydrogenase) (NAD(+)-specific
ICDH)
Length = 379
Score = 251 bits (642), Expect = 1e-66
Identities = 125/241 (51%), Positives = 164/241 (68%)
Frame = +1
Query: 4 IDFVVIREQTEGEYSALEHESVPGVVESLKIITKSKSERIAKFAFDFAKKHGRKKVTAVH 183
+DFV++REQTEGEYS+LEHE VPGV+E LKI T++K+ERIAKFAFD+A K GRKKVTAVH
Sbjct: 155 LDFVIVREQTEGEYSSLEHELVPGVIECLKISTRTKAERIAKFAFDYATKTGRKKVTAVH 214
Query: 184 KANIM*LVMXXXXXXXXXXXXKLGDGLFLESCRNVSKLYPDIKLDDIIVDNCCMQLVSNP 363
KANIM KLGDGLFL +C V+K YP I+ + +I+DN CMQLVS P
Sbjct: 215 KANIM----------------KLGDGLFLRTCEGVAKQYPKIQFESMIIDNTCMQLVSKP 258
Query: 364 QQFDVLVTPNLYGAVVDNXXXXXXXXXXXXXXXSFGYDVAIFEPGSRHPFAMAAGKNIAN 543
+QFDV+V PNLYG ++DN S G D IFEPGSRH F A G++IAN
Sbjct: 259 EQFDVMVMPNLYGNIIDNLAAGLVGGAGVVPGQSVGRDFVIFEPGSRHSFQEAMGRSIAN 318
Query: 544 PIGLFLSAVGMLHHINMHSQAIQLESAILNVLKDGEVRTEDIGGTNSTTEIVEAIISQVK 723
P + L A ML+H+++ + L A+ +V+K+G+VRT D+GG +T + +A+I + +
Sbjct: 319 PTAMILCAANMLNHLHLDAWGNSLRQAVADVVKEGKVRTRDLGGYATTVDFADAVIDKFR 378
Query: 724 I 726
I
Sbjct: 379 I 379
>sp|O94229|IDH1_KLULA Isocitrate dehydrogenase [NAD] subunit 1, mitochondrial precursor
(Isocitric dehydrogenase) (NAD(+)-specific ICDH)
Length = 361
Score = 224 bits (570), Expect = 3e-58
Identities = 118/241 (48%), Positives = 157/241 (65%), Gaps = 1/241 (0%)
Frame = +1
Query: 1 NIDFVVIREQTEGEYSALEHESVPGVVESLKIITKSKSERIAKFAFDFAKKHGRKKVTAV 180
NID V+IRE TEGEYS LEHESVPGVVESLKI+T++KSERIA+FAFDFA K+ RK V AV
Sbjct: 134 NIDMVIIRENTEGEYSGLEHESVPGVVESLKIMTRAKSERIARFAFDFALKNNRKSVCAV 193
Query: 181 HKANIM*LVMXXXXXXXXXXXXKLGDGLFLESCRNV-SKLYPDIKLDDIIVDNCCMQLVS 357
HKANIM KLGDGLF + + + YP++ + +IIVDN MQ V+
Sbjct: 194 HKANIM----------------KLGDGLFRNTVNEIGANEYPELDVKNIIVDNASMQAVA 237
Query: 358 NPQQFDVLVTPNLYGAVVDNXXXXXXXXXXXXXXXSFGYDVAIFEPGSRHPFAMAAGKNI 537
P QFDVLVTPNLYG+++ N +FG + A+FEPGSRH G+N+
Sbjct: 238 KPHQFDVLVTPNLYGSILGNIGSALIGGPGLVPGANFGREYAVFEPGSRHVGLDIKGQNV 297
Query: 538 ANPIGLFLSAVGMLHHINMHSQAIQLESAILNVLKDGEVRTEDIGGTNSTTEIVEAIISQ 717
ANP + LS+ ML H+ +++ A ++ A +V+ +G+ T DIGG+ ST+E A+I +
Sbjct: 298 ANPTAMILSSTLMLRHLGLNAYADRISKATYDVISEGKSTTRDIGGSASTSEFTNAVIEK 357
Query: 718 V 720
+
Sbjct: 358 L 358
>sp|P28834|IDH1_YEAST Isocitrate dehydrogenase [NAD] subunit 1, mitochondrial precursor
(Isocitric dehydrogenase) (NAD(+)-specific ICDH)
Length = 360
Score = 223 bits (569), Expect = 4e-58
Identities = 117/241 (48%), Positives = 155/241 (64%), Gaps = 1/241 (0%)
Frame = +1
Query: 1 NIDFVVIREQTEGEYSALEHESVPGVVESLKIITKSKSERIAKFAFDFAKKHGRKKVTAV 180
+ID +VIRE TEGE+S LEHESVPGVVESLK++T+ K+ERIA+FAFDFAKK+ RK VTAV
Sbjct: 133 DIDLIVIRENTEGEFSGLEHESVPGVVESLKVMTRPKTERIARFAFDFAKKYNRKSVTAV 192
Query: 181 HKANIM*LVMXXXXXXXXXXXXKLGDGLFLESCRNVS-KLYPDIKLDDIIVDNCCMQLVS 357
HKANIM KLGDGLF + K YPDI + IIVDN MQ V+
Sbjct: 193 HKANIM----------------KLGDGLFRNIITEIGQKEYPDIDVSSIIVDNASMQAVA 236
Query: 358 NPQQFDVLVTPNLYGAVVDNXXXXXXXXXXXXXXXSFGYDVAIFEPGSRHPFAMAAGKNI 537
P QFDVLVTP++YG ++ N +FG D A+FEPGSRH G+N+
Sbjct: 237 KPHQFDVLVTPSMYGTILGNIGAALIGGPGLVAGANFGRDYAVFEPGSRHVGLDIKGQNV 296
Query: 538 ANPIGLFLSAVGMLHHINMHSQAIQLESAILNVLKDGEVRTEDIGGTNSTTEIVEAIISQ 717
ANP + LS+ ML+H+ ++ A ++ A+ + +G+ T DIGG++STT+ II++
Sbjct: 297 ANPTAMILSSTLMLNHLGLNEYATRISKAVHETIAEGKHTTRDIGGSSSTTDFTNEIINK 356
Query: 718 V 720
+
Sbjct: 357 L 357
>sp|P41565|IDH3G_RAT Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial
precursor (Isocitric dehydrogenase) (NAD(+)-specific
ICDH)
Length = 393
Score = 222 bits (565), Expect = 1e-57
Identities = 117/242 (48%), Positives = 155/242 (64%)
Frame = +1
Query: 1 NIDFVVIREQTEGEYSALEHESVPGVVESLKIITKSKSERIAKFAFDFAKKHGRKKVTAV 180
+ID +++RE TEGEYS+LEHESV GVVESLKIITK+KS RIA++AF A++ GRKKVTAV
Sbjct: 160 DIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAV 219
Query: 181 HKANIM*LVMXXXXXXXXXXXXKLGDGLFLESCRNVSKLYPDIKLDDIIVDNCCMQLVSN 360
HKANIM KLGDGLFL+ CR V+ YP I D +IVDN MQLVS
Sbjct: 220 HKANIM----------------KLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSR 263
Query: 361 PQQFDVLVTPNLYGAVVDNXXXXXXXXXXXXXXXSFGYDVAIFEPGSRHPFAMAAGKNIA 540
PQQFDV+V PNLYG +V+N ++G+ A+FE +R+ A KNIA
Sbjct: 264 PQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIA 323
Query: 541 NPIGLFLSAVGMLHHINMHSQAIQLESAILNVLKDGEVRTEDIGGTNSTTEIVEAIISQV 720
NP L++ ML H+ +HS A + A+L + + + T DIGG +T++ ++ II +
Sbjct: 324 NPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSQAIQDIIRHI 383
Query: 721 KI 726
+I
Sbjct: 384 RI 385
>sp|P41564|IDH3G_MACFA Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial
precursor (Isocitric dehydrogenase) (NAD(+)-specific
ICDH)
Length = 355
Score = 221 bits (564), Expect = 2e-57
Identities = 116/242 (47%), Positives = 156/242 (64%)
Frame = +1
Query: 1 NIDFVVIREQTEGEYSALEHESVPGVVESLKIITKSKSERIAKFAFDFAKKHGRKKVTAV 180
+ID +++RE TEGEYS+LEHESV GVVESLKIITK+KS RIA++AF A++ GRKKVTAV
Sbjct: 122 DIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAV 181
Query: 181 HKANIM*LVMXXXXXXXXXXXXKLGDGLFLESCRNVSKLYPDIKLDDIIVDNCCMQLVSN 360
HKANIM KLGDGLFL+ CR V+ YP I +++IVDN MQLVS
Sbjct: 182 HKANIM----------------KLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSR 225
Query: 361 PQQFDVLVTPNLYGAVVDNXXXXXXXXXXXXXXXSFGYDVAIFEPGSRHPFAMAAGKNIA 540
PQQFDV+V PNLYG +V+N ++G+ A+FE +R+ A KNIA
Sbjct: 226 PQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIA 285
Query: 541 NPIGLFLSAVGMLHHINMHSQAIQLESAILNVLKDGEVRTEDIGGTNSTTEIVEAIISQV 720
NP L++ ML H+ +HS A + A+L + + + T DIGG +T+E ++ II +
Sbjct: 286 NPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSEAIQDIIRHI 345
Query: 721 KI 726
++
Sbjct: 346 RV 347
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 103,750,133
Number of Sequences: 369166
Number of extensions: 2211049
Number of successful extensions: 7869
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 7054
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7549
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8550075140
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)