Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_009_E10
(710 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P19966|TAGL_CHICK Transgelin (Smooth muscle protein 22-a... 82 1e-15
sp|Q24799|MYPH_ECHGR Myophilin 81 2e-15
sp|Q9UI15|TAGL3_HUMAN Transgelin-3 (Neuronal protein NP25) ... 76 8e-14
sp|Q08290|CNN1_RAT Calponin-1 (Calponin H1, smooth muscle) ... 76 1e-13
sp|Q08092|CNN1_PIG Calponin-1 (Calponin H1, smooth muscle) ... 76 1e-13
sp|Q9R1Q8|TAGL3_MOUSE Transgelin-3 (Neuronal protein NP25) 76 1e-13
sp|P37397|CNN3_RAT Calponin-3 (Calponin, acidic isoform) (C... 75 2e-13
sp|Q08091|CNN1_MOUSE Calponin-1 (Calponin H1, smooth muscle... 75 2e-13
sp|Q9DAW9|CNN3_MOUSE Calponin-3 (Calponin, acidic isoform) 75 2e-13
sp|Q15417|CNN3_HUMAN Calponin-3 (Calponin, acidic isoform) 74 3e-13
>sp|P19966|TAGL_CHICK Transgelin (Smooth muscle protein 22-alpha) (SM22-alpha)
Length = 200
Score = 82.4 bits (202), Expect = 1e-15
Identities = 46/110 (41%), Positives = 69/110 (62%), Gaps = 2/110 (1%)
Frame = +1
Query: 295 RQRERIGLFLGFCSKYGLKSSFEFQTDQLFEQTNLNQVIICLSQIDIEAQTKPN--YHGQ 468
+Q E+I FL YG+ + FQT LFE ++ V L + A TK + YHG
Sbjct: 89 KQMEQIAQFLKAAEDYGVVKTDMFQTVDLFEAKDMAAVQRTLVALGSLAVTKNDGHYHGD 148
Query: 469 KDFWIAKHQQNKRDFTEEQLRSGESIIGLQMGTNKTANASGVSFGAQRHI 618
++++ K Q++KR+F+E QL+ G++IIGLQMGTNK A+ +G+S+G R I
Sbjct: 149 PNWFMKKAQEHKREFSESQLKEGKNIIGLQMGTNKGASQAGMSYGRPRQI 198
>sp|Q24799|MYPH_ECHGR Myophilin
Length = 190
Score = 81.3 bits (199), Expect = 2e-15
Identities = 62/205 (30%), Positives = 94/205 (45%)
Frame = +1
Query: 10 SGFAKDVQEKLAAQFDKSEAAKTLRWIKSLNASVPPHIKAAFDKIPSNVQECAMDEXXXX 189
SG + V++KL + DK + + L WI++L K D + C +
Sbjct: 8 SGLSYQVKKKLEGKRDKDQENEALEWIEALTGLKLDRSKLYEDILKDGTVLCKLMNSIKP 67
Query: 190 XXXXXXXXXXXXALNSEKLNEKAWTIADKAIFETNRQRERIGLFLGFCSKYGLKSSFEFQ 369
+K+NE A T+ K + E I FL YG+ + FQ
Sbjct: 68 GCI-------------KKINENA-TMPFKIM-------ENISAFLEAMKGYGVPVADLFQ 106
Query: 370 TDQLFEQTNLNQVIICLSQIDIEAQTKPNYHGQKDFWIAKHQQNKRDFTEEQLRSGESII 549
T LFE+ ++ QV L + QT P Y G +NKR+FTE+QLR G++++
Sbjct: 107 TVDLFEKKDIAQVTRTLFALGRTCQTHPEYSGPV-LGPKLATENKREFTEQQLREGQNVV 165
Query: 550 GLQMGTNKTANASGVSFGAQRHITD 624
LQ G+NK A+ +G++ G QR I D
Sbjct: 166 SLQYGSNKGASQAGINMGKQRMIMD 190
>sp|Q9UI15|TAGL3_HUMAN Transgelin-3 (Neuronal protein NP25) (Neuronal protein 22) (NP22)
Length = 199
Score = 76.3 bits (186), Expect = 8e-14
Identities = 62/208 (29%), Positives = 101/208 (48%), Gaps = 6/208 (2%)
Frame = +1
Query: 13 GFAKDVQEKLAAQFDKSEAAKTLRWIK---SLNASVPPHIKAAFDKIPSNVQECAMDEXX 183
G +++VQEK+ ++D K + WI + + PP +A F Q+ MD
Sbjct: 9 GLSREVQEKIEQKYDADLENKLVDWIILQCAEDIEHPPPGRAHF-------QKWLMDGTV 61
Query: 184 XXXXXXXXXXXXXXALNSEKLNEKAWTIADKAIFETNRQRERIGLFLGFCSKYGLKSSFE 363
+ K++E +Q E+I FL YG++++
Sbjct: 62 LCKLINSLYPPGQEPI--PKISESKMAF---------KQMEQISQFLKAAETYGVRTTDI 110
Query: 364 FQTDQLFEQTNLNQVIICLSQIDIEAQTKPN--YHGQKDFWIAKHQQNKRDFTEEQLRSG 537
FQT L+E ++ V L + A TK + Y G+ ++ K QQN+R F+EEQLR G
Sbjct: 111 FQTVDLWEGKDMAAVQRTLMALGSVAVTKDDGCYRGEPSWFHRKAQQNRRGFSEEQLRQG 170
Query: 538 ESIIGLQMGTNKTANASGVS-FGAQRHI 618
+++IGLQMG+NK A+ +G++ +G R I
Sbjct: 171 QNVIGLQMGSNKGASQAGMTGYGMPRQI 198
>sp|Q08290|CNN1_RAT Calponin-1 (Calponin H1, smooth muscle) (Basic calponin)
Length = 297
Score = 75.9 bits (185), Expect = 1e-13
Identities = 60/208 (28%), Positives = 92/208 (44%), Gaps = 1/208 (0%)
Frame = +1
Query: 13 GFAKDVQEKLAAQFDKSEAAKTLRWIKSLNASVPPHIKAAFDKIPSNVQECAMDEXXXXX 192
G + +V+ KLA ++D + WI+ + +I SN + D
Sbjct: 13 GLSAEVKNKLAQKYDHQREQELREWIEGVTGR----------RIGSNFMDGLKDGIILCE 62
Query: 193 XXXXXXXXXXXALNSEKLNEKAWTIADKAIFETNRQRERIGLFLGFCSKYGLKSSFEFQT 372
+N N W Q E IG F+ +KYG+K F+
Sbjct: 63 FINKLQPGSVKKVNESTQN---W-----------HQLENIGNFIKAITKYGVKPHDIFEA 108
Query: 373 DQLFEQTNLNQVIICLSQIDIEAQTKPNYHGQKDFWIAKHQQNKRDFTEEQLRSGESIIG 552
+ LFE TN QV L + A+TK N + + + ++ +R F E+LR G +IIG
Sbjct: 109 NDLFENTNHTQVQSTLLALASMAKTKGN---KVNVGVKYAEKQERRFEPEKLREGRNIIG 165
Query: 553 LQMGTNKTANASGV-SFGAQRHITDSYL 633
LQMGTNK A+ G+ ++G +RH+ D L
Sbjct: 166 LQMGTNKFASQQGMTAYGTRRHLYDPKL 193
>sp|Q08092|CNN1_PIG Calponin-1 (Calponin H1, smooth muscle) (Basic calponin)
Length = 297
Score = 75.9 bits (185), Expect = 1e-13
Identities = 59/208 (28%), Positives = 93/208 (44%), Gaps = 1/208 (0%)
Frame = +1
Query: 13 GFAKDVQEKLAAQFDKSEAAKTLRWIKSLNASVPPHIKAAFDKIPSNVQECAMDEXXXXX 192
G + +V+ KLA ++D + + WI+ + +I +N + D
Sbjct: 13 GLSAEVKNKLAQKYDHQQEQELREWIEGVTGR----------RIGNNFMDGLKDGIILCE 62
Query: 193 XXXXXXXXXXXALNSEKLNEKAWTIADKAIFETNRQRERIGLFLGFCSKYGLKSSFEFQT 372
+N N W Q E IG F+ +KYG+K F+
Sbjct: 63 FINKLQPGSVKKVNESTQN---W-----------HQLENIGNFIKAITKYGVKPHDIFEA 108
Query: 373 DQLFEQTNLNQVIICLSQIDIEAQTKPNYHGQKDFWIAKHQQNKRDFTEEQLRSGESIIG 552
+ LFE TN QV L + A+TK N + + + ++ +R F E+LR G +IIG
Sbjct: 109 NDLFENTNHTQVQSTLLALASMAKTKGN---KVNVGVKYAEKQERKFEPEKLREGRNIIG 165
Query: 553 LQMGTNKTANASGV-SFGAQRHITDSYL 633
LQMGTNK A+ G+ ++G +RH+ D L
Sbjct: 166 LQMGTNKFASQQGMTAYGTRRHLYDPKL 193
>sp|Q9R1Q8|TAGL3_MOUSE Transgelin-3 (Neuronal protein NP25)
Length = 199
Score = 75.9 bits (185), Expect = 1e-13
Identities = 62/208 (29%), Positives = 101/208 (48%), Gaps = 6/208 (2%)
Frame = +1
Query: 13 GFAKDVQEKLAAQFDKSEAAKTLRWIK---SLNASVPPHIKAAFDKIPSNVQECAMDEXX 183
G +++VQEK+ ++D K + WI + + PP +A F Q+ MD
Sbjct: 9 GLSREVQEKIEQKYDADLENKLVDWIILQCAEDIEHPPPGRAHF-------QKWLMDGTV 61
Query: 184 XXXXXXXXXXXXXXALNSEKLNEKAWTIADKAIFETNRQRERIGLFLGFCSKYGLKSSFE 363
+ K++E +Q E+I FL YG++++
Sbjct: 62 LCKLINSLYPPGQEPI--PKISESKMAF---------KQMEQISQFLKAAEVYGVRTTDI 110
Query: 364 FQTDQLFEQTNLNQVIICLSQIDIEAQTKPN--YHGQKDFWIAKHQQNKRDFTEEQLRSG 537
FQT L+E ++ V L + A TK + Y G+ ++ K QQN+R F+EEQLR G
Sbjct: 111 FQTVDLWEGKDMAAVQRTLMALGSVAVTKDDGCYRGEPSWFHRKAQQNRRGFSEEQLRQG 170
Query: 538 ESIIGLQMGTNKTANASGVS-FGAQRHI 618
+++IGLQMG+NK A+ +G++ +G R I
Sbjct: 171 QNVIGLQMGSNKGASQAGMTGYGMPRQI 198
>sp|P37397|CNN3_RAT Calponin-3 (Calponin, acidic isoform) (Calponin, non-muscle
isoform)
Length = 330
Score = 75.1 bits (183), Expect = 2e-13
Identities = 57/205 (27%), Positives = 92/205 (44%), Gaps = 1/205 (0%)
Frame = +1
Query: 13 GFAKDVQEKLAAQFDKSEAAKTLRWIKSLNASVPPHIKAAFDKIPSNVQECAMDEXXXXX 192
G + +V+ K+A+++D+ WI+ + I +N Q D
Sbjct: 11 GLSAEVKNKIASKYDQQAEEDLRNWIEEVTGM----------GIGTNFQLGLKDGIILCE 60
Query: 193 XXXXXXXXXXXALNSEKLNEKAWTIADKAIFETNRQRERIGLFLGFCSKYGLKSSFEFQT 372
+N LN W Q E IG F+ YG+K F+
Sbjct: 61 LINKLQPGSVKKVNESSLN---WP-----------QLENIGNFIKAIQAYGMKPHDIFEA 106
Query: 373 DQLFEQTNLNQVIICLSQIDIEAQTKPNYHGQKDFWIAKHQQNKRDFTEEQLRSGESIIG 552
+ LFE N+ QV L + A+TK +H D + ++ R F E +L++G+S+IG
Sbjct: 107 NDLFENGNMTQVQTTLVALAGLAKTK-GFHTTIDIGVKYAEKQTRRFDEGKLKAGQSVIG 165
Query: 553 LQMGTNKTANASGV-SFGAQRHITD 624
LQMGTNK A+ +G+ ++G +RH+ D
Sbjct: 166 LQMGTNKCASQAGMTAYGTRRHLYD 190
Score = 30.0 bits (66), Expect = 6.3
Identities = 18/45 (40%), Positives = 26/45 (57%), Gaps = 4/45 (8%)
Frame = +1
Query: 502 KRDFTEEQLRS---GESIIGLQMGTNKTANASGVS-FGAQRHITD 624
+RD +++L S I LQMGTNK A+ G+S +G R + D
Sbjct: 225 RRDIYDQKLTLQPVDNSTISLQMGTNKVASQKGMSVYGLGRQVYD 269
>sp|Q08091|CNN1_MOUSE Calponin-1 (Calponin H1, smooth muscle) (Basic calponin)
Length = 297
Score = 74.7 bits (182), Expect = 2e-13
Identities = 59/208 (28%), Positives = 92/208 (44%), Gaps = 1/208 (0%)
Frame = +1
Query: 13 GFAKDVQEKLAAQFDKSEAAKTLRWIKSLNASVPPHIKAAFDKIPSNVQECAMDEXXXXX 192
G + +V+ KLA ++D + WI+ + +I +N + D
Sbjct: 13 GLSAEVKNKLAQKYDHQREQELREWIEGVTGR----------RIGNNFMDGLKDGIILCE 62
Query: 193 XXXXXXXXXXXALNSEKLNEKAWTIADKAIFETNRQRERIGLFLGFCSKYGLKSSFEFQT 372
+N N W Q E IG F+ +KYG+K F+
Sbjct: 63 FINKLQPGSVKKVNESTQN---W-----------HQLENIGNFIKAITKYGVKPHDIFEA 108
Query: 373 DQLFEQTNLNQVIICLSQIDIEAQTKPNYHGQKDFWIAKHQQNKRDFTEEQLRSGESIIG 552
+ LFE TN QV L + A+TK N + + + ++ +R F E+LR G +IIG
Sbjct: 109 NDLFENTNHTQVQSTLLALASMAKTKGN---KVNVGVKYAEKQERRFEPEKLREGRNIIG 165
Query: 553 LQMGTNKTANASGV-SFGAQRHITDSYL 633
LQMGTNK A+ G+ ++G +RH+ D L
Sbjct: 166 LQMGTNKFASQQGMTAYGTRRHLYDPKL 193
>sp|Q9DAW9|CNN3_MOUSE Calponin-3 (Calponin, acidic isoform)
Length = 330
Score = 74.7 bits (182), Expect = 2e-13
Identities = 57/205 (27%), Positives = 92/205 (44%), Gaps = 1/205 (0%)
Frame = +1
Query: 13 GFAKDVQEKLAAQFDKSEAAKTLRWIKSLNASVPPHIKAAFDKIPSNVQECAMDEXXXXX 192
G + +V+ K+A+++D+ WI+ + I +N Q D
Sbjct: 11 GLSAEVKNKIASKYDQQAEEDLRNWIEEVTGL----------GIGTNFQLGLKDGIILCE 60
Query: 193 XXXXXXXXXXXALNSEKLNEKAWTIADKAIFETNRQRERIGLFLGFCSKYGLKSSFEFQT 372
+N LN W Q E IG F+ YG+K F+
Sbjct: 61 LINKLQPGSVKKVNESSLN---WP-----------QLENIGNFIKAIQAYGMKPHDIFEA 106
Query: 373 DQLFEQTNLNQVIICLSQIDIEAQTKPNYHGQKDFWIAKHQQNKRDFTEEQLRSGESIIG 552
+ LFE N+ QV L + A+TK +H D + ++ R F E +L++G+S+IG
Sbjct: 107 NDLFENGNMTQVQTTLVALAGLAKTK-GFHTTIDIGVKYAEKQTRRFDEGKLKAGQSVIG 165
Query: 553 LQMGTNKTANASGV-SFGAQRHITD 624
LQMGTNK A+ +G+ ++G +RH+ D
Sbjct: 166 LQMGTNKCASQAGMTAYGTRRHLYD 190
Score = 30.4 bits (67), Expect = 4.9
Identities = 28/92 (30%), Positives = 41/92 (44%), Gaps = 4/92 (4%)
Frame = +1
Query: 361 EFQTDQLFEQTNLNQVIICLSQIDIEAQTKPNYHGQKDFWIAKHQQNKRDFTEEQLRS-- 534
+ QTD+ F+QT I Q N + +A +RD +++L
Sbjct: 192 KMQTDKPFDQTT------------ISLQMGTNKGASQAGMLAPG--TRRDIYDQKLTLQP 237
Query: 535 -GESIIGLQMGTNKTANASGVS-FGAQRHITD 624
S I LQMGTNK A+ G+S +G R + D
Sbjct: 238 VDNSTISLQMGTNKVASQKGMSVYGLGRQVYD 269
>sp|Q15417|CNN3_HUMAN Calponin-3 (Calponin, acidic isoform)
Length = 329
Score = 74.3 bits (181), Expect = 3e-13
Identities = 57/205 (27%), Positives = 90/205 (43%), Gaps = 1/205 (0%)
Frame = +1
Query: 13 GFAKDVQEKLAAQFDKSEAAKTLRWIKSLNASVPPHIKAAFDKIPSNVQECAMDEXXXXX 192
G + +V+ K+A+++D WI+ + I N Q D
Sbjct: 11 GLSAEVKNKIASKYDHQAEEDLRNWIEEVTGM----------SIGPNFQLGLKDGIILCE 60
Query: 193 XXXXXXXXXXXALNSEKLNEKAWTIADKAIFETNRQRERIGLFLGFCSKYGLKSSFEFQT 372
+N LN W Q E IG F+ YG+K F+
Sbjct: 61 LINKLQPGSVKKVNESSLN---WP-----------QLENIGNFIKAIQAYGMKPHDIFEA 106
Query: 373 DQLFEQTNLNQVIICLSQIDIEAQTKPNYHGQKDFWIAKHQQNKRDFTEEQLRSGESIIG 552
+ LFE N+ QV L + A+TK +H D + ++ R F E +L++G+S+IG
Sbjct: 107 NDLFENGNMTQVQTTLVALAGLAKTK-GFHTTIDIGVKYAEKQTRRFDEGKLKAGQSVIG 165
Query: 553 LQMGTNKTANASGV-SFGAQRHITD 624
LQMGTNK A+ +G+ ++G +RH+ D
Sbjct: 166 LQMGTNKCASQAGMTAYGTRRHLYD 190
Score = 30.4 bits (67), Expect = 4.9
Identities = 28/92 (30%), Positives = 41/92 (44%), Gaps = 4/92 (4%)
Frame = +1
Query: 361 EFQTDQLFEQTNLNQVIICLSQIDIEAQTKPNYHGQKDFWIAKHQQNKRDFTEEQLRS-- 534
+ QTD+ F+QT I Q N + +A +RD +++L
Sbjct: 192 KMQTDKPFDQTT------------ISLQMGTNKGASQAGMLAPG--TRRDIYDQKLTLQP 237
Query: 535 -GESIIGLQMGTNKTANASGVS-FGAQRHITD 624
S I LQMGTNK A+ G+S +G R + D
Sbjct: 238 VDNSTISLQMGTNKVASQKGMSVYGLGRQVYD 269
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 68,408,490
Number of Sequences: 369166
Number of extensions: 1187140
Number of successful extensions: 2629
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2529
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2602
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6267895215
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)