Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_009_E03
(418 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q99KI0|ACON_MOUSE Aconitate hydratase, mitochondrial pre... 146 1e-35
sp|P16276|ACON_PIG Aconitate hydratase, mitochondrial precu... 145 2e-35
sp|Q9ER34|ACON_RAT Aconitate hydratase, mitochondrial precu... 145 2e-35
sp|P20004|ACON_BOVIN Aconitate hydratase, mitochondrial pre... 145 4e-35
sp|Q99798|ACON_HUMAN Aconitate hydratase, mitochondrial pre... 144 7e-35
sp|P34455|ACON_CAEEL Probable aconitate hydratase, mitochon... 140 1e-33
sp|P49609|ACON_GRAVE Aconitate hydratase, mitochondrial pre... 122 4e-28
sp|O13966|ACON_SCHPO Aconitate hydratase, mitochondrial pre... 115 4e-26
sp|P19414|ACON_YEAST Aconitate hydratase, mitochondrial pre... 115 4e-26
sp|P39533|ACON2_YEAST Putative aconitase in PRP21-UBP12 int... 92 4e-19
>sp|Q99KI0|ACON_MOUSE Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 780
Score = 146 bits (369), Expect = 1e-35
Identities = 67/97 (69%), Positives = 81/97 (83%)
Frame = +1
Query: 1 GRVIIVKSFARIHETNLKKQGMLPLTFSKPEDYNKIQPSDKISLLNLAKFAPEKPVECEI 180
GR II KSFARIHETNLKKQG+LPLTF+ P DYNKI P DK+++ L FAP KP++C I
Sbjct: 683 GRAIITKSFARIHETNLKKQGLLPLTFADPSDYNKIHPVDKLTIQGLKDFAPGKPLKCVI 742
Query: 181 KHKDGTKEVITLNHTFNKNQIDWFKAGSALNRMRELK 291
KH +GT+E I LNHTFN+ QI+WF+AGSALNRM+EL+
Sbjct: 743 KHPNGTQETILLNHTFNETQIEWFRAGSALNRMKELQ 779
>sp|P16276|ACON_PIG Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 781
Score = 145 bits (367), Expect = 2e-35
Identities = 67/97 (69%), Positives = 81/97 (83%)
Frame = +1
Query: 1 GRVIIVKSFARIHETNLKKQGMLPLTFSKPEDYNKIQPSDKISLLNLAKFAPEKPVECEI 180
GR II KSFARIHETNLKKQG+LPLTF+ P DYNKI P DK+++ L FAP KP++C I
Sbjct: 683 GRAIITKSFARIHETNLKKQGLLPLTFADPADYNKIHPVDKLTIQGLKDFAPGKPLKCII 742
Query: 181 KHKDGTKEVITLNHTFNKNQIDWFKAGSALNRMRELK 291
KH +GT+E I LNHTFN+ QI+WF+AGSALNRM+EL+
Sbjct: 743 KHPNGTQETILLNHTFNETQIEWFRAGSALNRMKELQ 779
>sp|Q9ER34|ACON_RAT Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 780
Score = 145 bits (367), Expect = 2e-35
Identities = 67/97 (69%), Positives = 80/97 (82%)
Frame = +1
Query: 1 GRVIIVKSFARIHETNLKKQGMLPLTFSKPEDYNKIQPSDKISLLNLAKFAPEKPVECEI 180
GR II KSFARIHETNLKKQG+LPLTF+ P DYNKI P DK+++ L FAP KP+ C I
Sbjct: 683 GRAIITKSFARIHETNLKKQGLLPLTFADPSDYNKIHPVDKLTIQGLKDFAPGKPLNCII 742
Query: 181 KHKDGTKEVITLNHTFNKNQIDWFKAGSALNRMRELK 291
KH +GT+E I LNHTFN+ QI+WF+AGSALNRM+EL+
Sbjct: 743 KHPNGTQETILLNHTFNETQIEWFRAGSALNRMKELQ 779
>sp|P20004|ACON_BOVIN Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 780
Score = 145 bits (365), Expect = 4e-35
Identities = 67/97 (69%), Positives = 80/97 (82%)
Frame = +1
Query: 1 GRVIIVKSFARIHETNLKKQGMLPLTFSKPEDYNKIQPSDKISLLNLAKFAPEKPVECEI 180
GR II KSFARIHETNLKKQG+LPLTF+ P DYNKI P DK+++ L FAP KP+ C I
Sbjct: 683 GRAIITKSFARIHETNLKKQGLLPLTFADPADYNKIHPVDKLTIKGLKDFAPGKPLTCII 742
Query: 181 KHKDGTKEVITLNHTFNKNQIDWFKAGSALNRMRELK 291
KH +GT+E I LNHTFN+ QI+WF+AGSALNRM+EL+
Sbjct: 743 KHPNGTQETILLNHTFNETQIEWFRAGSALNRMKELQ 779
>sp|Q99798|ACON_HUMAN Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 780
Score = 144 bits (363), Expect = 7e-35
Identities = 66/97 (68%), Positives = 80/97 (82%)
Frame = +1
Query: 1 GRVIIVKSFARIHETNLKKQGMLPLTFSKPEDYNKIQPSDKISLLNLAKFAPEKPVECEI 180
GR II KSFARIHETNLKKQG+LPLTF+ P DYNKI P DK+++ L F P KP++C I
Sbjct: 683 GRAIITKSFARIHETNLKKQGLLPLTFADPADYNKIHPVDKLTIQGLKDFTPGKPLKCII 742
Query: 181 KHKDGTKEVITLNHTFNKNQIDWFKAGSALNRMRELK 291
KH +GT+E I LNHTFN+ QI+WF+AGSALNRM+EL+
Sbjct: 743 KHPNGTQETILLNHTFNETQIEWFRAGSALNRMKELQ 779
>sp|P34455|ACON_CAEEL Probable aconitate hydratase, mitochondrial precursor (Citrate
hydro-lyase) (Aconitase)
Length = 777
Score = 140 bits (352), Expect = 1e-33
Identities = 65/96 (67%), Positives = 79/96 (82%)
Frame = +1
Query: 1 GRVIIVKSFARIHETNLKKQGMLPLTFSKPEDYNKIQPSDKISLLNLAKFAPEKPVECEI 180
GR IIVKSFARIHETNLKKQGMLPLTF+ P DY+KI PSD +S++ L+ FAP KP+
Sbjct: 677 GRAIIVKSFARIHETNLKKQGMLPLTFANPADYDKIDPSDNVSIVGLSSFAPGKPLTAIF 736
Query: 181 KHKDGTKEVITLNHTFNKNQIDWFKAGSALNRMREL 288
K +G+K +TLNHTFN+ QI+WFKAGSALNRM+E+
Sbjct: 737 KKTNGSKVEVTLNHTFNEQQIEWFKAGSALNRMKEV 772
>sp|P49609|ACON_GRAVE Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 779
Score = 122 bits (305), Expect = 4e-28
Identities = 57/94 (60%), Positives = 70/94 (74%)
Frame = +1
Query: 1 GRVIIVKSFARIHETNLKKQGMLPLTFSKPEDYNKIQPSDKISLLNLAKFAPEKPVECEI 180
G +IVKSFARIHETNLKKQGMLPLTF+ P DY+KI SDK+SL+ L P +PV +
Sbjct: 681 GVAVIVKSFARIHETNLKKQGMLPLTFNNPADYDKIDSSDKVSLVGLKNLTPGEPVTMTV 740
Query: 181 KHKDGTKEVITLNHTFNKNQIDWFKAGSALNRMR 282
DGT I LNHTFN Q++WF+AGSALN+++
Sbjct: 741 TKADGTSMDILLNHTFNDEQLEWFRAGSALNKIK 774
>sp|O13966|ACON_SCHPO Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 778
Score = 115 bits (288), Expect = 4e-26
Identities = 57/97 (58%), Positives = 69/97 (71%), Gaps = 1/97 (1%)
Frame = +1
Query: 1 GRVIIVKSFARIHETNLKKQGMLPLTFSKPEDYNKIQPSDKISLLNLAKFAPEKPVECEI 180
G +I KSFARIHETNLKKQG+LPLTF+ P Y+KI P D + + L FAP KP+ +
Sbjct: 678 GAAVITKSFARIHETNLKKQGLLPLTFADPAAYDKISPFDTVDIDGLTTFAPGKPLTLVV 737
Query: 181 KHKDGTKEVIT-LNHTFNKNQIDWFKAGSALNRMREL 288
DG+ E T LNHTFNK+QI+WFKAGSALN M +
Sbjct: 738 HPADGSAEWSTKLNHTFNKDQIEWFKAGSALNHMANM 774
>sp|P19414|ACON_YEAST Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 778
Score = 115 bits (288), Expect = 4e-26
Identities = 55/94 (58%), Positives = 66/94 (70%)
Frame = +1
Query: 1 GRVIIVKSFARIHETNLKKQGMLPLTFSKPEDYNKIQPSDKISLLNLAKFAPEKPVECEI 180
G II KSFARIHETNLKKQG+LPL F P DY+KI P D+I +L LA+ AP KPV +
Sbjct: 680 GFAIITKSFARIHETNLKKQGLLPLNFKNPADYDKINPDDRIDILGLAELAPGKPVTMRV 739
Query: 181 KHKDGTKEVITLNHTFNKNQIDWFKAGSALNRMR 282
K+G L HTFN QI+WFK GSALN+++
Sbjct: 740 HPKNGKPWDAVLTHTFNDEQIEWFKYGSALNKIK 773
>sp|P39533|ACON2_YEAST Putative aconitase in PRP21-UBP12 intergenic region
Length = 789
Score = 92.0 bits (227), Expect = 4e-19
Identities = 46/102 (45%), Positives = 65/102 (63%), Gaps = 5/102 (4%)
Frame = +1
Query: 1 GRVIIVKSFARIHETNLKKQGMLPLTFSKPEDYNKIQPSDKISLLNLAKFAPEK-----P 165
G +++VKSFARIHETNLKKQG+LPLTF+ DY+KI D + LNL +
Sbjct: 685 GEILLVKSFARIHETNLKKQGVLPLTFANESDYDKISSGDVLETLNLVDMIAKDGNNGGE 744
Query: 166 VECEIKHKDGTKEVITLNHTFNKNQIDWFKAGSALNRMRELK 291
++ +I +G I HT +K+QID+FKAGSA+N + ++
Sbjct: 745 IDVKITKPNGESFTIKAKHTMSKDQIDFFKAGSAINYIGNIR 786
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 40,866,853
Number of Sequences: 369166
Number of extensions: 710735
Number of successful extensions: 2403
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2354
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2399
length of database: 68,354,980
effective HSP length: 99
effective length of database: 50,066,215
effective search space used: 1952582385
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)