Planarian EST Database


Dr_sW_009_C09

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_009_C09
         (808 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O08730|GLYG_RAT  Glycogenin-1                                   84   6e-16
sp|P13280|GLYG_RABIT  Glycogenin-1                                 81   4e-15
sp|Q9R062|GLYG_MOUSE  Glycogenin-1                                 80   5e-15
sp|P46976|GLYG_HUMAN  Glycogenin-1                                 76   1e-13
sp|O15488|GLYG2_HUMAN  Glycogenin-2 (GN-2) (GN2)                   57   6e-08
sp|P36143|GLG1_YEAST  Glycogen synthesis initiator protein GLG1    39   0.022
sp|P47011|GLG2_YEAST  Glycogen synthesis initiator protein GLG2    33   1.2  
sp|Q88YK3|HPRK_LACPL  HPr kinase/phosphorylase (HPrK/P) (HPr...    32   2.7  
sp|P16534|HLYD_PASHA  Leukotoxin secretion protein D               30   7.8  
sp|P55125|HLYD_PASSP  Leukotoxin secretion protein D               30   7.8  
>sp|O08730|GLYG_RAT Glycogenin-1
          Length = 333

 Score = 83.6 bits (205), Expect = 6e-16
 Identities = 50/142 (35%), Positives = 70/142 (49%), Gaps = 14/142 (9%)
 Frame = +3

Query: 6   SQAFYSYLPAYTHFRADIKVLHFIGPHKPWHHTFDSDTGTV--IFQEGCGQNYESLQLWW 179
           S + YSYLPA+  F  + KV+HF+G  KPW++T++  T +V    Q+    + E L LWW
Sbjct: 191 SLSIYSYLPAFKAFGKNAKVVHFLGRTKPWNYTYNPQTKSVKCESQDPIVSHPEFLNLWW 250

Query: 180 TTFIAYTKPLLHDE-----------MGGVCGRMASLDVSSFVP-HEGKFSPKSHQQAWEH 323
            TF     PLL              M  V G ++ L      P  +   S +  ++ WE 
Sbjct: 251 DTFTTNVLPLLQHHGLVKDAGSYLMMEHVTGALSDLSFGEAPPASQPSLSSEERKERWEQ 310

Query: 324 GIIDYRGLDRFENIKMHLDSKL 389
           G  DY G D F+NIK  LD+ L
Sbjct: 311 GQADYMGADSFDNIKRKLDTYL 332
>sp|P13280|GLYG_RABIT Glycogenin-1
          Length = 333

 Score = 80.9 bits (198), Expect = 4e-15
 Identities = 49/142 (34%), Positives = 71/142 (50%), Gaps = 14/142 (9%)
 Frame = +3

Query: 6   SQAFYSYLPAYTHFRADIKVLHFIGPHKPWHHTFDSDTGTVIFQ--EGCGQNYESLQLWW 179
           S + YSYLPA+  F A+ KV+HF+G  KPW++T+D+ T +V  +  +    + + L +WW
Sbjct: 191 SISIYSYLPAFKAFGANAKVVHFLGQTKPWNYTYDTKTKSVRSEGHDPTMTHPQFLNVWW 250

Query: 180 TTFIAYTKPLLHD--EMGGVCGRMASLDVSSFVPH----------EGKFSPKSHQQAWEH 323
             F     PLL     +   C      DVS  V H          +   S +  ++ WE 
Sbjct: 251 DIFTTSVVPLLQQFGLVQDTCSYQHVEDVSGAVSHLSLGETPATTQPFVSSEERKERWEQ 310

Query: 324 GIIDYRGLDRFENIKMHLDSKL 389
           G  DY G D F+NIK  LD+ L
Sbjct: 311 GQADYMGADSFDNIKKKLDTYL 332
>sp|Q9R062|GLYG_MOUSE Glycogenin-1
          Length = 333

 Score = 80.5 bits (197), Expect = 5e-15
 Identities = 49/142 (34%), Positives = 69/142 (48%), Gaps = 14/142 (9%)
 Frame = +3

Query: 6   SQAFYSYLPAYTHFRADIKVLHFIGPHKPWHHTFDSDTGTV--IFQEGCGQNYESLQLWW 179
           S + YSYLPA+  F  + KV+HF+G  KPW++T++  T +V    Q+    + E L LWW
Sbjct: 191 SISIYSYLPAFKAFGKNAKVVHFLGRTKPWNYTYNPQTKSVNCDSQDPTVSHPEFLNLWW 250

Query: 180 TTFIAYTKPLLHDE-----------MGGVCGRMASLDVSSF-VPHEGKFSPKSHQQAWEH 323
            TF     PLL              M  V G ++ L         +   S +  ++ WE 
Sbjct: 251 DTFTTNVLPLLQHHGLVKDASSYLMMEHVSGALSDLSFGEAPAAPQPSMSSEERKERWEQ 310

Query: 324 GIIDYRGLDRFENIKMHLDSKL 389
           G  DY G D F+NIK  LD+ L
Sbjct: 311 GQADYMGADSFDNIKRKLDTYL 332
>sp|P46976|GLYG_HUMAN Glycogenin-1
          Length = 350

 Score = 75.9 bits (185), Expect = 1e-13
 Identities = 53/160 (33%), Positives = 69/160 (43%), Gaps = 32/160 (20%)
 Frame = +3

Query: 6   SQAFYSYLPAYTHFRADIKVLHFIGPHKPWHHTFDSDTGTVIFQEGCGQNY---ESLQLW 176
           S + YSYLPA+  F A  KV+HF+G  KPW++T+D  T +V   E    N    E L LW
Sbjct: 191 SISIYSYLPAFKVFGASAKVVHFLGRVKPWNYTYDPKTKSV-KSEAHDPNMTHPEFLILW 249

Query: 177 WTTFIAYTKPLLHD-------------------EMGGVCGRMASLDVSSFVPH------- 278
           W  F     PLL                      +   CG     DVS  + H       
Sbjct: 250 WNIFTTNVLPLLQQFGLVKDTCSYVNVLSDLVYTLAFSCGFCRKEDVSGAISHLSLGEIP 309

Query: 279 ---EGKFSPKSHQQAWEHGIIDYRGLDRFENIKMHLDSKL 389
              +   S +  ++ WE G  DY G D F+NIK  LD+ L
Sbjct: 310 AMAQPFVSSEERKERWEQGQADYMGADSFDNIKRKLDTYL 349
>sp|O15488|GLYG2_HUMAN Glycogenin-2 (GN-2) (GN2)
          Length = 501

 Score = 57.0 bits (136), Expect = 6e-08
 Identities = 30/102 (29%), Positives = 46/102 (45%), Gaps = 20/102 (19%)
 Frame = +3

Query: 6   SQAFYSYLPAYTHFRADIKVLHFIGPHKPWHHTFDSDTGTVIFQEGCGQNYES---LQLW 176
           S   Y+Y PA+  F +  KV+HF+G  KPW++ ++  +G+V+ Q     +      L LW
Sbjct: 224 SNTMYTYSPAFKQFGSSAKVVHFLGSMKPWNYKYNPQSGSVLEQGSVSSSQHQAAFLHLW 283

Query: 177 WTTFIAYTKP-----------------LLHDEMGGVCGRMAS 251
           WT +     P                 L H ++GG C   AS
Sbjct: 284 WTVYQNNVLPLYKSVQAGEARASPGHTLCHSDVGGPCADSAS 325

 Score = 33.5 bits (75), Expect = 0.70
 Identities = 14/32 (43%), Positives = 19/32 (59%)
 Frame = +3

Query: 285 KFSPKSHQQAWEHGIIDYRGLDRFENIKMHLD 380
           + SP+  ++ WE G IDY G D F  I+  LD
Sbjct: 466 ELSPEEERRKWEEGRIDYMGKDAFARIQEKLD 497
>sp|P36143|GLG1_YEAST Glycogen synthesis initiator protein GLG1
          Length = 480

 Score = 38.5 bits (88), Expect = 0.022
 Identities = 14/26 (53%), Positives = 18/26 (69%)
 Frame = +3

Query: 18  YSYLPAYTHFRADIKVLHFIGPHKPW 95
           Y   PA  +F+  IK++HFIG HKPW
Sbjct: 94  YQSSPAMNYFKPSIKLIHFIGKHKPW 119
>sp|P47011|GLG2_YEAST Glycogen synthesis initiator protein GLG2
          Length = 380

 Score = 32.7 bits (73), Expect = 1.2
 Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
 Frame = +3

Query: 18  YSYLPAYTHFRADIKVLHFIGPHKPW-HHTFDSD 116
           Y   PA   F+  I+++HFIG  KPW  +T D D
Sbjct: 232 YQSSPAMNFFQQHIRLIHFIGTFKPWSRNTTDYD 265
>sp|Q88YK3|HPRK_LACPL HPr kinase/phosphorylase (HPrK/P) (HPr(Ser) kinase/phosphorylase)
          Length = 315

 Score = 31.6 bits (70), Expect = 2.7
 Identities = 25/83 (30%), Positives = 36/83 (43%), Gaps = 5/83 (6%)
 Frame = +3

Query: 159 ESLQLWWTTFIAYTKPLLHDEMGGVCGRMASLDVSSFVPHEGKFSPKSHQQAWEHGIIDY 338
           E +QL   T  AY+K + HDE   +  +M  L   +FV   G   P+   QA E   +  
Sbjct: 52  ERVQLLGKTETAYSKNMSHDERLMIFRKMCQLTTPAFVISTGLPVPEELVQAGEENGVPI 111

Query: 339 RGLDR-----FENIKMHLDSKLA 392
            G          N+  +L+ KLA
Sbjct: 112 LGTKMTSSRILSNMTNYLEGKLA 134
>sp|P16534|HLYD_PASHA Leukotoxin secretion protein D
          Length = 478

 Score = 30.0 bits (66), Expect = 7.8
 Identities = 14/34 (41%), Positives = 24/34 (70%)
 Frame = -1

Query: 808 RFGKSIITIKIKRNIE*SRQLAIDVINNNTQKQA 707
           +F KS +  K+K++IE  RQL +++  NN ++QA
Sbjct: 293 QFFKSDVLEKLKQHIENERQLRLELEKNNQRRQA 326
>sp|P55125|HLYD_PASSP Leukotoxin secretion protein D
          Length = 478

 Score = 30.0 bits (66), Expect = 7.8
 Identities = 14/34 (41%), Positives = 24/34 (70%)
 Frame = -1

Query: 808 RFGKSIITIKIKRNIE*SRQLAIDVINNNTQKQA 707
           +F KS +  K+K++IE  RQL +++  NN ++QA
Sbjct: 293 QFFKSDVLEKLKQHIENERQLQLELEKNNQRRQA 326
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85,153,770
Number of Sequences: 369166
Number of extensions: 1695168
Number of successful extensions: 5131
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4082
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4862
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7666799535
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)