Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_009_C08
(729 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P10961|HSF_YEAST Heat shock factor protein (HSF) (Heat s... 34 0.35
sp|O14248|TEA3_SCHPO Tip elongation aberrant protein 3 (Cel... 34 0.35
sp|Q7VA20|IF2_PROMA Translation initiation factor IF-2 34 0.45
sp|P54677|PI4K_DICDI Phosphatidylinositol 4-kinase (PI4-kin... 33 0.78
sp|P54675|PI3K3_DICDI Phosphatidylinositol 3-kinase 3 (PI3-... 33 0.78
sp|Q46089|FTSK_CAMJE DNA translocase ftsK 33 1.0
sp|Q91YE5|BAZ2A_MOUSE Bromodomain adjacent to zinc finger d... 33 1.0
sp|P10849|NAM1_YEAST NAM1 protein, mitochondrial precursor ... 32 1.3
sp|O14157|MYO3_SCHPO Myosin type II heavy chain 2 32 1.3
sp|Q47502|CEAK_ECOLI Colicin-K 32 1.7
>sp|P10961|HSF_YEAST Heat shock factor protein (HSF) (Heat shock transcription factor)
(HSTF)
Length = 833
Score = 34.3 bits (77), Expect = 0.35
Identities = 47/204 (23%), Positives = 80/204 (39%), Gaps = 24/204 (11%)
Frame = +2
Query: 59 QKISLAIKKSNAFAHINPSDLIRKRYANTNFNSISEIP-------SKKFKAVETSDPKSF 217
Q+ L + +++ + NPS L + N ISEIP F+ + DP +
Sbjct: 535 QRYLLKNRANSSTSSENPS-LTPFDIESNNDRKISEIPFDDEEEEETDFRPFTSRDPNNQ 593
Query: 218 KENTCLIPDLMSILPDPDLLFHTSPNYNKSLVKSECIVKDN------------SIIERKV 361
P+ ++L D DL + N NK + DN +E V
Sbjct: 594 TSENTFDPNRFTMLSDDDLKKDSHTNDNKH--NESDLFWDNVHRNIDEQDARLQNLENMV 651
Query: 362 HGV-----NKTNNNSISSQSIDVDKELTISVNLTAPPVSNISTFDSNSTKLNNCFSSDNK 526
H + NK+ NN SS + + + E ++VN + + T +SNS + S+
Sbjct: 652 HILSPGYPNKSFNNKTSSTNTNSNMESAVNVNSPGFNLQDYLTGESNSPNSVHSVPSNGS 711
Query: 527 DKSPKREVNREKQNKDLSNLATSI 598
+P N + D + +TS+
Sbjct: 712 GSTPLPMPN----DNDTEHASTSV 731
>sp|O14248|TEA3_SCHPO Tip elongation aberrant protein 3 (Cell polarity protein tea3)
Length = 1125
Score = 34.3 bits (77), Expect = 0.35
Identities = 43/211 (20%), Positives = 87/211 (41%), Gaps = 6/211 (2%)
Frame = +2
Query: 50 LLCQKISLAIKKSNAFAHINPSDLIRKRYANTNFNSISEIPSKKFKAVETSDPKSFKENT 229
L C + +K N F H++ + K+ + + I + +E+++ K K+
Sbjct: 557 LYCMQQGYTLKPPNLFQHVDEKLRLEKKEQLSYLEILKVIE----QMLESNEQKFKKQIV 612
Query: 230 CLIPDLMSILPDPDLLFHTSPNYNKSLVKSECIVKDNSIIERKV----HGVNKTNNNSIS 397
L + + D + NY++SL++ + + + KV + V T + S
Sbjct: 613 SLASENAKLAAQRDAAVENA-NYSRSLIQKKTTDETVGSLIEKVGKLEYEVQGTLEEATS 671
Query: 398 SQSIDVDKELTISVNLTAPPV--SNISTFDSNSTKLNNCFSSDNKDKSPKREVNREKQNK 571
+ + + + N +A + S + KL + F D+ + ++ +K+N+
Sbjct: 672 YYQKNTELQQLLKQNESASELLKSRNEKLCVDYDKLRSVFEEDSS-----KILSLQKENE 726
Query: 572 DLSNLATSILPLPTQLFGKHSMLTALEKGNY 664
NL + IL + +L S ALE GNY
Sbjct: 727 ---NLQSQILQISEELVDYRSRCEALEYGNY 754
>sp|Q7VA20|IF2_PROMA Translation initiation factor IF-2
Length = 1134
Score = 33.9 bits (76), Expect = 0.45
Identities = 26/109 (23%), Positives = 51/109 (46%), Gaps = 1/109 (0%)
Frame = +2
Query: 317 SECIVKDNSIIERKVHGVNKTNNNSISSQSIDVDKE-LTISVNLTAPPVSNISTFDSNST 493
S I +++ +K+ K++N+S +KE LT+ +T+PP + + +N++
Sbjct: 38 SSSISDEDATKIKKLLLAQKSSNSSSPPAKQKPNKEILTLKKAITSPPTKSEANAKTNAS 97
Query: 494 KLNNCFSSDNKDKSPKREVNREKQNKDLSNLATSILPLPTQLFGKHSML 640
L+ S NK SPK+E+ + + + PT K++ L
Sbjct: 98 -LDKTSSLKNKPASPKKEIPTSPKPPSAAKQRVDAIKKPTPSISKNNSL 145
>sp|P54677|PI4K_DICDI Phosphatidylinositol 4-kinase (PI4-kinase) (PtdIns-4-kinase)
(PI4K-alpha)
Length = 1093
Score = 33.1 bits (74), Expect = 0.78
Identities = 28/97 (28%), Positives = 40/97 (41%)
Frame = +2
Query: 293 NYNKSLVKSECIVKDNSIIERKVHGVNKTNNNSISSQSIDVDKELTISVNLTAPPVSNIS 472
N N ++ +E DNSI + N NNN+IS + + + I +N SNI
Sbjct: 203 NNNNEILPNEN--SDNSINDENNQYGNSNNNNNISGE----NNNIKIDINSQNKSDSNIE 256
Query: 473 TFDSNSTKLNNCFSSDNKDKSPKREVNREKQNKDLSN 583
T NST +S KD N N + +N
Sbjct: 257 TL--NSTLCEETKTSPIKDDMENNNNNNNNNNNNNNN 291
>sp|P54675|PI3K3_DICDI Phosphatidylinositol 3-kinase 3 (PI3-kinase) (PtdIns-3-kinase)
(PI3K)
Length = 1585
Score = 33.1 bits (74), Expect = 0.78
Identities = 29/129 (22%), Positives = 58/129 (44%), Gaps = 6/129 (4%)
Frame = +2
Query: 293 NYNKSLVKSECIVKDNSIIERKVHGVNKTNNNSISSQSIDVDKELTISVNLTAPPVSNIS 472
N N + + I+ S ++ + N +NNN+ + ++ D +T +N+T ++ I
Sbjct: 75 NNNNNNNNNNVIIPSASTENKEENDNNNSNNNN--NINLSPDSSITKDINITENKITEIK 132
Query: 473 TFDSNSTKLNNCFSSDNKDK----SPKR-EVNREKQNKDLSNLA-TSILPLPTQLFGKHS 634
T ++ T + K SPK+ E E + + ++N+A T+ P+ L
Sbjct: 133 TTETKETSTGTSPLEKSPSKGFIISPKKPEEENEIEGETINNIAITNYTQGPSMLTLMKK 192
Query: 635 MLTALEKGN 661
L ++K N
Sbjct: 193 KLENIKKNN 201
Score = 30.8 bits (68), Expect = 3.9
Identities = 24/67 (35%), Positives = 36/67 (53%), Gaps = 2/67 (2%)
Frame = +2
Query: 380 NNNSISSQS-IDVDKELTISVNLTAPPVSNISTFD-SNSTKLNNCFSSDNKDKSPKREVN 553
N N SSQS +D+ + ++ + S+ S D S S K NN DNK+K ++E
Sbjct: 1503 NKNKPSSQSKLDLSRS---DLSRSDSSRSDSSRLDLSRSDKKNN---KDNKEKEKEKEKE 1556
Query: 554 REKQNKD 574
+EK+N D
Sbjct: 1557 KEKENND 1563
>sp|Q46089|FTSK_CAMJE DNA translocase ftsK
Length = 946
Score = 32.7 bits (73), Expect = 1.0
Identities = 45/194 (23%), Positives = 81/194 (41%), Gaps = 13/194 (6%)
Frame = +2
Query: 44 DELLCQKISLAIKKSNAFAHINPSDLIRKRYANTNFNSISEIPSKKFKAVETSDPKSFKE 223
+E+L K+ IKK + IN SD+I +FNS I ++ E SD + +
Sbjct: 337 NEILENKVKQEIKKLEEYDAINSSDIIEGN--KYSFNSPKTIKTE----TEESDKINENK 390
Query: 224 NTCLIPDLMSILPDPDLLFHTSPNYNKSLVK--SECIVKDNSII------ERKVHGVNKT 379
N ++ P + L H P + +K +E ++++ + + E K+ KT
Sbjct: 391 NLDKADNIFEFAPIVEELNH--PYIEPTPIKNINEIVIEEKNTLDFIQNTETKIDN-EKT 447
Query: 380 NNNSISSQSIDVDKELTISVNLTAPPVSNISTFDSNSTKLNNCFSSDNKD-----KSPKR 544
N+ I Q + KE+ I+ L + + + F+ D K K+
Sbjct: 448 NDQEIKLQKAVLAKEIAINQAL-------LREIEQGEIEKPKDFTLPPLDFLANPKEHKQ 500
Query: 545 EVNREKQNKDLSNL 586
E+N + +K + NL
Sbjct: 501 EINESEIDKKIYNL 514
>sp|Q91YE5|BAZ2A_MOUSE Bromodomain adjacent to zinc finger domain 2A (Transcription
termination factor I-interacting protein 5)
(TTF-I-interacting protein 5) (Tip5)
Length = 1850
Score = 32.7 bits (73), Expect = 1.0
Identities = 17/51 (33%), Positives = 27/51 (52%)
Frame = +2
Query: 377 TNNNSISSQSIDVDKELTISVNLTAPPVSNISTFDSNSTKLNNCFSSDNKD 529
+N N+ S + D +I V+ T+PPV S NS LN+C S+ ++
Sbjct: 307 SNANAFSLLADDSQTSASIFVSPTSPPVLGESVLQDNSFGLNSCSDSEQEE 357
>sp|P10849|NAM1_YEAST NAM1 protein, mitochondrial precursor (Mitochondrial transcription
factor 2)
Length = 440
Score = 32.3 bits (72), Expect = 1.3
Identities = 22/104 (21%), Positives = 49/104 (47%)
Frame = +2
Query: 293 NYNKSLVKSECIVKDNSIIERKVHGVNKTNNNSISSQSIDVDKELTISVNLTAPPVSNIS 472
NY C++ + ++ +RK H V + +N Q+++ ++++ + V N+
Sbjct: 12 NYRYIHCIHRCLLNEANLKDRKTHNVERVSNEKTFEQALEEERKVFGELFEAGARVENMR 71
Query: 473 TFDSNSTKLNNCFSSDNKDKSPKREVNREKQNKDLSNLATSILP 604
+N++K+ + + + +D S V +EK + S A LP
Sbjct: 72 --HTNASKIIDKYYNGLQDNSEGTSVKKEKIVFNHSQRAQRKLP 113
>sp|O14157|MYO3_SCHPO Myosin type II heavy chain 2
Length = 2104
Score = 32.3 bits (72), Expect = 1.3
Identities = 22/122 (18%), Positives = 59/122 (48%), Gaps = 4/122 (3%)
Frame = +2
Query: 296 YNKSLVKSECIVKDNSIIERKVHGVNKTNNNSISSQSIDVDKELTISVNLTAPPVSNIST 475
+++ +++ ++K+NSI++ + +++ NN+S ++Q + L+++ L +N +
Sbjct: 1785 FDELVLEKTNLLKENSILQADLQSLSRVNNSSSTAQQNAQSQLLSLTAQLQEVREANQTL 1844
Query: 476 FDSNSTKLNNCFSSDNKDKSPKREVNREKQNK----DLSNLATSILPLPTQLFGKHSMLT 643
T L + + K ++N++ + D + +L L + L K +L+
Sbjct: 1845 RKDQDTLLRENRNLERKLHEVSEQLNKKFDSSARPFDEIEMEKEVLTLKSNLAQKDDLLS 1904
Query: 644 AL 649
+L
Sbjct: 1905 SL 1906
>sp|Q47502|CEAK_ECOLI Colicin-K
Length = 548
Score = 32.0 bits (71), Expect = 1.7
Identities = 23/86 (26%), Positives = 43/86 (50%), Gaps = 6/86 (6%)
Frame = +2
Query: 332 KDNSIIERKVHGVNKTNNNSIS------SQSIDVDKELTISVNLTAPPVSNISTFDSNST 493
+++++++ GV++ N+ IS ++S + T SVN TAP + +S D +S
Sbjct: 94 ENSTLVDNPFGGVSRVLNSLISDMPSLFAESSGNNNNNTASVN-TAPTNAQVSDMDKSSK 152
Query: 494 KLNNCFSSDNKDKSPKREVNREKQNK 571
++N + K K+ EKQ K
Sbjct: 153 VVSNVINEKQKQKNKIATQISEKQKK 178
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 78,284,140
Number of Sequences: 369166
Number of extensions: 1484254
Number of successful extensions: 4795
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4631
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4788
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6486082400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)