Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_009_C05
(315 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P68586|MTBP_BPPHT Modification methylase Phi3TI (Cytosin... 33 0.20
sp||P09389_1 [Segment 1 of 2] Modification methylase SPBeta... 33 0.20
sp|P53855|ATG2_YEAST Autophagy-related protein 2 (Sporulati... 29 2.9
sp|Q57532|GYRA_MYCLE DNA gyrase subunit A [Contains: Mle gy... 29 2.9
sp|P17632|MBHL_RHOGE Uptake hydrogenase large subunit (Hydr... 29 3.8
sp|P31891|MBHL_RALEU Uptake hydrogenase large subunit (Hydr... 28 5.0
sp|P12636|MBHL_BRAJA Uptake hydrogenase large subunit (Hydr... 28 6.5
sp|P18636|MBHL_RHILV Uptake hydrogenase large subunit (Hydr... 28 6.5
sp|P75318|Y465_MYCPN Hypothetical protein MPN465 (P01_orf199) 28 6.5
sp|Q83B24|SYM_COXBU Methionyl-tRNA synthetase (Methionine--... 28 6.5
>sp|P68586|MTBP_BPPHT Modification methylase Phi3TI (Cytosine-specific methyltransferase
Phi3TI) (M.Phi3TI)
sp|P68585|MTBP_BACSU Phi-3T prophage-derived modification methylase Phi3TI
(Cytosine-specific methyltransferase Phi3TI) (M.Phi3TI)
Length = 443
Score = 33.1 bits (74), Expect = 0.20
Identities = 14/30 (46%), Positives = 20/30 (66%), Gaps = 2/30 (6%)
Frame = -3
Query: 133 CSSCSF--IIRWESYRRFVKCPMCNLLPKA 50
CS+CS +I +E Y++ VKCP C + KA
Sbjct: 94 CSNCSHEHLITYEDYKKGVKCPKCEAVSKA 123
>sp||P09389_1 [Segment 1 of 2] Modification methylase SPBetaI (Cytosine-specific
methyltransferase SPBetaI) (M.SPBetaI)
Length = 143
Score = 33.1 bits (74), Expect = 0.20
Identities = 14/30 (46%), Positives = 20/30 (66%), Gaps = 2/30 (6%)
Frame = -3
Query: 133 CSSCSF--IIRWESYRRFVKCPMCNLLPKA 50
CS+CS +I +E Y++ VKCP C + KA
Sbjct: 94 CSNCSHEHLITYEDYKKGVKCPKCEAVSKA 123
>sp|P53855|ATG2_YEAST Autophagy-related protein 2 (Sporulation-specific protein 72)
Length = 1592
Score = 29.3 bits (64), Expect = 2.9
Identities = 23/65 (35%), Positives = 27/65 (41%), Gaps = 13/65 (20%)
Frame = -3
Query: 160 TLGLPWSLICSSCSFII---RWESYRRFVK----------CPMCNLLPKATRTKLFII*D 20
T G PW L C CS I+ R +S V P LL KA LF+I D
Sbjct: 778 TSGFPWELKCLDCSLILHPFRLKSVMVIVLDNLTTGGSSFIPQAKLLSKA--NTLFLIDD 835
Query: 19 YYRFR 5
Y F+
Sbjct: 836 YQNFK 840
>sp|Q57532|GYRA_MYCLE DNA gyrase subunit A [Contains: Mle gyrA intein]
Length = 1273
Score = 29.3 bits (64), Expect = 2.9
Identities = 18/44 (40%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
Frame = +3
Query: 33 NSLVRVAFGKRLHIGHF-TKRRYDSQRIIKEQLEQINDHGKPSV 161
NSLVR+ FGK + IG T +++S I L+ ++ HG P V
Sbjct: 135 NSLVRLLFGKSIRIGDIVTGAQFNSDNPI--DLKVLDRHGNPVV 176
>sp|P17632|MBHL_RHOGE Uptake hydrogenase large subunit (Hydrogenlyase) (Membrane-bound
hydrogenase large subunit)
Length = 618
Score = 28.9 bits (63), Expect = 3.8
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = +3
Query: 132 QINDHGKPSVHLHALFWVASISGL 203
Q++DH HLHAL WV +S L
Sbjct: 114 QVHDHAVHFYHLHALDWVDVVSAL 137
>sp|P31891|MBHL_RALEU Uptake hydrogenase large subunit (Hydrogenlyase) (Membrane-bound
hydrogenase large subunit)
Length = 618
Score = 28.5 bits (62), Expect = 5.0
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = +3
Query: 108 RIIKEQLEQINDHGKPSVHLHALFWVASISGL 203
R I + Q++DH HLHAL WV +S L
Sbjct: 106 REIMAKTLQVHDHAVHFYHLHALDWVDVMSAL 137
>sp|P12636|MBHL_BRAJA Uptake hydrogenase large subunit (Hydrogenlyase) (Membrane-bound
hydrogenase large subunit)
Length = 596
Score = 28.1 bits (61), Expect = 6.5
Identities = 14/35 (40%), Positives = 20/35 (57%)
Frame = +3
Query: 99 DSQRIIKEQLEQINDHGKPSVHLHALFWVASISGL 203
+S R + + Q++DH HLHAL WV +S L
Sbjct: 103 NSIRNLMQLALQVHDHVVHFYHLHALDWVDVVSAL 137
>sp|P18636|MBHL_RHILV Uptake hydrogenase large subunit (Hydrogenlyase) (Membrane-bound
hydrogenase large subunit)
Length = 596
Score = 28.1 bits (61), Expect = 6.5
Identities = 14/35 (40%), Positives = 20/35 (57%)
Frame = +3
Query: 99 DSQRIIKEQLEQINDHGKPSVHLHALFWVASISGL 203
+S R + + Q++DH HLHAL WV +S L
Sbjct: 103 NSIRNLMQLALQVHDHVVHFYHLHALDWVDVVSAL 137
>sp|P75318|Y465_MYCPN Hypothetical protein MPN465 (P01_orf199)
Length = 199
Score = 28.1 bits (61), Expect = 6.5
Identities = 12/27 (44%), Positives = 16/27 (59%), Gaps = 1/27 (3%)
Frame = -3
Query: 175 SACKCTLGL-PWSLICSSCSFIIRWES 98
SAC+ L + PW+ ICS +F W S
Sbjct: 46 SACRIDLSVFPWAFICSPWNFCSTWSS 72
>sp|Q83B24|SYM_COXBU Methionyl-tRNA synthetase (Methionine--tRNA ligase) (MetRS)
Length = 546
Score = 28.1 bits (61), Expect = 6.5
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = +3
Query: 123 QLEQINDHGKPSVHLHALFWVASISG 200
Q E + GK V+ HALFW A +SG
Sbjct: 286 QTELYHFVGKDIVYFHALFWPAMLSG 311
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 36,492,086
Number of Sequences: 369166
Number of extensions: 694888
Number of successful extensions: 1699
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1678
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1699
length of database: 68,354,980
effective HSP length: 73
effective length of database: 54,869,325
effective search space used: 1700949075
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)